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The map label for this gene is nifA [H]

Identifier: 85860380

GI number: 85860380

Start: 2553567

End: 2554934

Strand: Direct

Name: nifA [H]

Synonym: SYN_01181

Alternate gene names: 85860380

Gene position: 2553567-2554934 (Clockwise)

Preceding gene: 85860379

Following gene: 85860381

Centisome position: 80.32

GC content: 48.9

Gene sequence:

>1368_bases
ATGAGTAAAGATATTGAAGATCAGACGCAGATCATTCTTGACAGCATTGCAGATGGCGTTTTTACCGTTGATTCTGAATG
GAAAATCACATCTTTCAACCGCGCAGCGGAGAAGATAACCGGCATAGCCAGGGAAGAGGCCATAGGGAAGCAATGCCGGG
AGGTTTTCAGGGCCGGCATCTGTGAAAACCATTGCTTTCTAAGGCACACCGTCGAGAGCGGACAGCCCGTCGTCAACCAA
TCCATATTTATCCTGAATGCGAGGGGAGAGCGCATCCCCGTAAGCATCTCCACGGCCCTCCTTAAAAACAGGGAAAAACA
GGTGATCGGCGGGGTCGAGACATTCAGGGATCTCAGTCTGGTGGAAGATCTGCGCAAGGAACTGTACAACCGCCGCTCCT
TTCATGACATCATCAGCAAAAACAAAGAGATGCATCGTCTTTTCCATATTGTGGAGCAATTGTCCGAAAGCGATGCCACC
GTCATTCTTGTCGGCGAAAGCGGCACAGGAAAGGAACTGTTTGCAAAGGCCATTCATTACGAGAGTTCAAGAAGGGATGG
CCCCATCATAACAATCAACTGCGGCGCTCTGCCGGACAATCTCCTTGAGTCAGAGCTTTTCGGCTATAAAGCAGGAGCCT
TTACAGACGCCAAAAGGGATAAGCCCGGACGTATAACCCTGGCTCATGGCGGAACGCTTTTTCTCGATGAGATCGGTGAT
ATCTCCCAGGCCATGCAGGTCAGACTCCTGCGCGTCCTCCAGGAGAAGGTTTTTGAACCTCTTGGAGGGACGAAATCGGA
AAAAGTCGACGTCCGGATCATTGCGGCAACCAATAAGGATCTGGAGAAGCTTGTAAAAGAAGGGATTTTTCGTGAAGATT
TCTACTACAGAATCAATGTGGTCAAACTTCTTCTTCCTCCCTTGAGGGATCGTAAAGAGGATATTCCTCTCTTATCGGAA
CACTTCATCCGAAAATTAAACCGGCGCAAGGGGACGGACATTCTGGGACTCCAACCCGAGGCCCTGGCAATCCTTATGGC
ATATGACTATCCCGGCAATATCCGGGAACTGGAAAATATGATCGAATACGCGATGGTCGTCTGTAAAAACAGTCTCATCG
GCATCGAGCATCTCCCCGATTACCTGATCCAGAGTTTAGACCATGGCAGTCAAAAATCATTTCTCCCGAAACAAAAGCAG
ATTTCATTGAAGGGTCATGAAAGAGAATTCCTTTATAACGCACTGGCCGAAAACAATTGGAACAGAAAACGCACCGCAGC
CCAATTGGGCATACATCCCACGACCCTCTGGCGAAAGCTCAGGAGACTGAATATTGAGCTTCCAGAACAGGATGGACGCC
ATAAGTAA

Upstream 100 bases:

>100_bases
ATCCGGACAATCAGCAGAATACAGGATAAACCAGAAACCGGAAACTGGGAGAAATCAGGGAATCAATTCCCGAGCGGACA
GGACAAAATCAACCCCTGAG

Downstream 100 bases:

>100_bases
TTTATGTCCTATGTCCTTCGAAAAAGATCGTTGAAATAATATTGCAACATTGCATTATTGCCCACACACAATTGCATTAT
TGCAAACACCCTAAAATCCG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSKDIEDQTQIILDSIADGVFTVDSEWKITSFNRAAEKITGIAREEAIGKQCREVFRAGICENHCFLRHTVESGQPVVNQ
SIFILNARGERIPVSISTALLKNREKQVIGGVETFRDLSLVEDLRKELYNRRSFHDIISKNKEMHRLFHIVEQLSESDAT
VILVGESGTGKELFAKAIHYESSRRDGPIITINCGALPDNLLESELFGYKAGAFTDAKRDKPGRITLAHGGTLFLDEIGD
ISQAMQVRLLRVLQEKVFEPLGGTKSEKVDVRIIAATNKDLEKLVKEGIFREDFYYRINVVKLLLPPLRDRKEDIPLLSE
HFIRKLNRRKGTDILGLQPEALAILMAYDYPGNIRELENMIEYAMVVCKNSLIGIEHLPDYLIQSLDHGSQKSFLPKQKQ
ISLKGHEREFLYNALAENNWNRKRTAAQLGIHPTTLWRKLRRLNIELPEQDGRHK

Sequences:

>Translated_455_residues
MSKDIEDQTQIILDSIADGVFTVDSEWKITSFNRAAEKITGIAREEAIGKQCREVFRAGICENHCFLRHTVESGQPVVNQ
SIFILNARGERIPVSISTALLKNREKQVIGGVETFRDLSLVEDLRKELYNRRSFHDIISKNKEMHRLFHIVEQLSESDAT
VILVGESGTGKELFAKAIHYESSRRDGPIITINCGALPDNLLESELFGYKAGAFTDAKRDKPGRITLAHGGTLFLDEIGD
ISQAMQVRLLRVLQEKVFEPLGGTKSEKVDVRIIAATNKDLEKLVKEGIFREDFYYRINVVKLLLPPLRDRKEDIPLLSE
HFIRKLNRRKGTDILGLQPEALAILMAYDYPGNIRELENMIEYAMVVCKNSLIGIEHLPDYLIQSLDHGSQKSFLPKQKQ
ISLKGHEREFLYNALAENNWNRKRTAAQLGIHPTTLWRKLRRLNIELPEQDGRHK
>Mature_454_residues
SKDIEDQTQIILDSIADGVFTVDSEWKITSFNRAAEKITGIAREEAIGKQCREVFRAGICENHCFLRHTVESGQPVVNQS
IFILNARGERIPVSISTALLKNREKQVIGGVETFRDLSLVEDLRKELYNRRSFHDIISKNKEMHRLFHIVEQLSESDATV
ILVGESGTGKELFAKAIHYESSRRDGPIITINCGALPDNLLESELFGYKAGAFTDAKRDKPGRITLAHGGTLFLDEIGDI
SQAMQVRLLRVLQEKVFEPLGGTKSEKVDVRIIAATNKDLEKLVKEGIFREDFYYRINVVKLLLPPLRDRKEDIPLLSEH
FIRKLNRRKGTDILGLQPEALAILMAYDYPGNIRELENMIEYAMVVCKNSLIGIEHLPDYLIQSLDHGSQKSFLPKQKQI
SLKGHEREFLYNALAENNWNRKRTAAQLGIHPTTLWRKLRRLNIELPEQDGRHK

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=329, Percent_Identity=43.7689969604863, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI87082117, Length=367, Percent_Identity=41.6893732970027, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1790437, Length=307, Percent_Identity=45.2768729641694, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1789233, Length=470, Percent_Identity=34.8936170212766, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1789087, Length=328, Percent_Identity=42.6829268292683, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=42.2960725075529, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1788905, Length=309, Percent_Identity=41.1003236245955, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1786524, Length=333, Percent_Identity=38.7387387387387, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI87082152, Length=321, Percent_Identity=38.006230529595, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=291, Percent_Identity=38.8316151202749, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1787583, Length=435, Percent_Identity=31.7241379310345, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI87081858, Length=454, Percent_Identity=30.1762114537445, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789828, Length=267, Percent_Identity=32.9588014981273, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51890; Mature: 51759

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDIEDQTQIILDSIADGVFTVDSEWKITSFNRAAEKITGIAREEAIGKQCREVFRAGI
CCCCCHHHHHHHHHHHCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CENHCFLRHTVESGQPVVNQSIFILNARGERIPVSISTALLKNREKQVIGGVETFRDLSL
CCCCHHEEEEHHCCCCEECCEEEEEECCCCEECEEHHHHHHHCCHHHHHHHHHHHHHHHH
VEDLRKELYNRRSFHDIISKNKEMHRLFHIVEQLSESDATVILVGESGTGKELFAKAIHY
HHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
ESSRRDGPIITINCGALPDNLLESELFGYKAGAFTDAKRDKPGRITLAHGGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHCC
ISQAMQVRLLRVLQEKVFEPLGGTKSEKVDVRIIAATNKDLEKLVKEGIFREDFYYRINV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHCCCCCCCHHHHHHH
VKLLLPPLRDRKEDIPLLSEHFIRKLNRRKGTDILGLQPEALAILMAYDYPGNIRELENM
HHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCHHHEEEEEEECCCCCHHHHHHH
IEYAMVVCKNSLIGIEHLPDYLIQSLDHGSQKSFLPKQKQISLKGHEREFLYNALAENNW
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCC
NRKRTAAQLGIHPTTLWRKLRRLNIELPEQDGRHK
CHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SKDIEDQTQIILDSIADGVFTVDSEWKITSFNRAAEKITGIAREEAIGKQCREVFRAGI
CCCCHHHHHHHHHHHCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CENHCFLRHTVESGQPVVNQSIFILNARGERIPVSISTALLKNREKQVIGGVETFRDLSL
CCCCHHEEEEHHCCCCEECCEEEEEECCCCEECEEHHHHHHHCCHHHHHHHHHHHHHHHH
VEDLRKELYNRRSFHDIISKNKEMHRLFHIVEQLSESDATVILVGESGTGKELFAKAIHY
HHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
ESSRRDGPIITINCGALPDNLLESELFGYKAGAFTDAKRDKPGRITLAHGGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHCC
ISQAMQVRLLRVLQEKVFEPLGGTKSEKVDVRIIAATNKDLEKLVKEGIFREDFYYRINV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHCCCCCCCHHHHHHH
VKLLLPPLRDRKEDIPLLSEHFIRKLNRRKGTDILGLQPEALAILMAYDYPGNIRELENM
HHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCHHHEEEEEEECCCCCHHHHHHH
IEYAMVVCKNSLIGIEHLPDYLIQSLDHGSQKSFLPKQKQISLKGHEREFLYNALAENNW
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCC
NRKRTAAQLGIHPTTLWRKLRRLNIELPEQDGRHK
CHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]