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The map label for this gene is rpfG [H]

Identifier: 85860311

GI number: 85860311

Start: 2491484

End: 2493274

Strand: Direct

Name: rpfG [H]

Synonym: SYN_01234

Alternate gene names: 85860311

Gene position: 2491484-2493274 (Clockwise)

Preceding gene: 85860309

Following gene: 85860318

Centisome position: 78.37

GC content: 52.48

Gene sequence:

>1791_bases
TTGAAAGAGATCATTCATGAATTCAAGTGGATTTGCCATATGGATCTAACCAGCCGGATACTGATTGTCGATGATCTGCC
CCAGGAGCGCTGGATGCTCGGCGCCCTGCTGAAATCTTATGATTATGAATTGAGCTACGCGACGAACGGCCATGAAGCGC
TGGAATTAGCAAAGAAAATCAATCCGGATTTGATCCTGCTCGATGTGATGATGCCGGGGATGGACGGGTTCGAAGTTTGC
CGTCGTATCCGCAAGACCCCCCTGATCGCTGAGGTTCCGATTATGATGGTAACGGCCCTGAATGAGGCCGAAGTTCGCCG
GAAGGGCGTTGAAATCGGCGCTGATGATTTCATCTCCAAACCTTTCGACCGCGCCGAACTTCAGGCACGCGTCCGCAACA
TCACACGGCTCAATCGCCACCGCCGACATCGTCTGCATGAAGAACGAGTCATCCAGGAAAAACTGTTCAACGCCCTCCCT
GACGGCTTGATGGTTCTTGCTGAAAACGGGACCATTCAGATCGTCAATCCCGCTCTGCGCCGGATGCTCTTGTATCGGGA
TTCCACAGAACTTATGGGCAGGACACTCAGTGAACTGCTCGCGCCTGATTATCGGGAAACCTTCGAGGGGTCATTCCACC
GATGCCTCAGCGATCCTTCCTGCATGGAAAACAATCAACTCGATTTTCTGCGCCGCGATGGATCCAGAATGACCGCCGAG
CTCACTGTCACCTCTCTGGCAGGAGAAGGGATGCGTGCATTGCAGGTGATCCTGAGGGACATAACAGACCGCATCAAAAC
GGAGAGCAGGCTTCGGGAGTCGGAAGAGCAGTACCGGGTGCTGTTCATGGGCAGCACGCAGGGCATCCTGGCCACCGATA
TGGAAACGAAGCGTTTTACGTTTGCCAATCCGGCGGTCTGCCGGATGCTCGGATATTCCGAGGAAGAATTGCTGCGGTTG
GGTTTAGCGGACATCCACCCGAGAAACTTTCTTGACTGCGCTGTAATCGAATTCGAGCTCCAGGTACGGGGCGAAAGAGA
GATTTCCTCCGGAATTCCCTATTTACGGAAGGACGGCACCGTTTTTTACGCTGATACAAGCGGTTCTAAAACCCTGTTCA
AGGGACGGGAATGCCACGTAAGCTTTCTGACGGAAGTGACTCAGCGCAAACTGGCAGAGGAGACCCTTGAACTTACCCTC
AATAATCTGAAGAAGGCTTTTGCGGGTGCCATTCAGGTCATGGTGTCGGCAGTAGAGGCGAGAGATTCCTATACGGCCGG
TCATCAACGCCGGACCGCGGACCTTGCCTCTACAATAGCAGGGGAGATGGGATTGTCCCAGGAAAAAATCGACGCCATCC
GCATGGCCGGTTCCATTCATGACATCGGAAAACTGTTCGTCCCGTCGGAGATTTTGTGCAAGCCGGCAAAACTGTTCGAT
ATCGAGTTTACATTGATTAAAGAACACCCCAGACAAGGATTCGAGATTCTGAAGGATGTGGATTTTCCCTGGCCGCTGGC
GGATATTATTTACCAGCACCATGAACGCATAGATGGCTCCGGCTATCCAAGAAATCTGAAAGGAGATGACATTCTCGTCG
AGGCCCGCATTCTTTCTGTTGCCGACACGGTGGAAGCCATGGCCTCCCACAGACCTTATCGTCCCGGTCTGGGCCTCGAT
CTGGCTCTGGAAGAAATCGAGAAGAACAGGGGGATTTTTTACGACTGTGTTGCCGTAGATGCCTGTTTAAGATTATTTCG
GGAAAAAGGCTATAACTTTTCCCTGACCTGA

Upstream 100 bases:

>100_bases
TTTCTTTCAGGCTCTCTCCAGGAAAGGACTTGTTGCGAGAAAAAAGGCTGGATTTTGAATCACGGAAAAAAGAGAGTTTG
TCTTCATCCCCTCACACAGT

Downstream 100 bases:

>100_bases
TGCATACATAAAGAAATTGCCAAGGTTTTATGCTTATTCGACATCCGGAAGCCTCGGGATTGTTCAATTGGATTTAAAGC
GCCATGCAGCAAAATTAATA

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKEIIHEFKWICHMDLTSRILIVDDLPQERWMLGALLKSYDYELSYATNGHEALELAKKINPDLILLDVMMPGMDGFEVC
RRIRKTPLIAEVPIMMVTALNEAEVRRKGVEIGADDFISKPFDRAELQARVRNITRLNRHRRHRLHEERVIQEKLFNALP
DGLMVLAENGTIQIVNPALRRMLLYRDSTELMGRTLSELLAPDYRETFEGSFHRCLSDPSCMENNQLDFLRRDGSRMTAE
LTVTSLAGEGMRALQVILRDITDRIKTESRLRESEEQYRVLFMGSTQGILATDMETKRFTFANPAVCRMLGYSEEELLRL
GLADIHPRNFLDCAVIEFELQVRGEREISSGIPYLRKDGTVFYADTSGSKTLFKGRECHVSFLTEVTQRKLAEETLELTL
NNLKKAFAGAIQVMVSAVEARDSYTAGHQRRTADLASTIAGEMGLSQEKIDAIRMAGSIHDIGKLFVPSEILCKPAKLFD
IEFTLIKEHPRQGFEILKDVDFPWPLADIIYQHHERIDGSGYPRNLKGDDILVEARILSVADTVEAMASHRPYRPGLGLD
LALEEIEKNRGIFYDCVAVDACLRLFREKGYNFSLT

Sequences:

>Translated_596_residues
MKEIIHEFKWICHMDLTSRILIVDDLPQERWMLGALLKSYDYELSYATNGHEALELAKKINPDLILLDVMMPGMDGFEVC
RRIRKTPLIAEVPIMMVTALNEAEVRRKGVEIGADDFISKPFDRAELQARVRNITRLNRHRRHRLHEERVIQEKLFNALP
DGLMVLAENGTIQIVNPALRRMLLYRDSTELMGRTLSELLAPDYRETFEGSFHRCLSDPSCMENNQLDFLRRDGSRMTAE
LTVTSLAGEGMRALQVILRDITDRIKTESRLRESEEQYRVLFMGSTQGILATDMETKRFTFANPAVCRMLGYSEEELLRL
GLADIHPRNFLDCAVIEFELQVRGEREISSGIPYLRKDGTVFYADTSGSKTLFKGRECHVSFLTEVTQRKLAEETLELTL
NNLKKAFAGAIQVMVSAVEARDSYTAGHQRRTADLASTIAGEMGLSQEKIDAIRMAGSIHDIGKLFVPSEILCKPAKLFD
IEFTLIKEHPRQGFEILKDVDFPWPLADIIYQHHERIDGSGYPRNLKGDDILVEARILSVADTVEAMASHRPYRPGLGLD
LALEEIEKNRGIFYDCVAVDACLRLFREKGYNFSLT
>Mature_596_residues
MKEIIHEFKWICHMDLTSRILIVDDLPQERWMLGALLKSYDYELSYATNGHEALELAKKINPDLILLDVMMPGMDGFEVC
RRIRKTPLIAEVPIMMVTALNEAEVRRKGVEIGADDFISKPFDRAELQARVRNITRLNRHRRHRLHEERVIQEKLFNALP
DGLMVLAENGTIQIVNPALRRMLLYRDSTELMGRTLSELLAPDYRETFEGSFHRCLSDPSCMENNQLDFLRRDGSRMTAE
LTVTSLAGEGMRALQVILRDITDRIKTESRLRESEEQYRVLFMGSTQGILATDMETKRFTFANPAVCRMLGYSEEELLRL
GLADIHPRNFLDCAVIEFELQVRGEREISSGIPYLRKDGTVFYADTSGSKTLFKGRECHVSFLTEVTQRKLAEETLELTL
NNLKKAFAGAIQVMVSAVEARDSYTAGHQRRTADLASTIAGEMGLSQEKIDAIRMAGSIHDIGKLFVPSEILCKPAKLFD
IEFTLIKEHPRQGFEILKDVDFPWPLADIIYQHHERIDGSGYPRNLKGDDILVEARILSVADTVEAMASHRPYRPGLGLD
LALEEIEKNRGIFYDCVAVDACLRLFREKGYNFSLT

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=153, Percent_Identity=39.2156862745098, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=35.5371900826446, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=39.0243902439024, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=38.9830508474576, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788550, Length=133, Percent_Identity=34.5864661654135, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1789149, Length=113, Percent_Identity=34.5132743362832, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI87082012, Length=121, Percent_Identity=33.8842975206612, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790863, Length=142, Percent_Identity=30.9859154929577, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790860, Length=101, Percent_Identity=37.6237623762376, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI145693157, Length=102, Percent_Identity=40.1960784313725, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790437, Length=110, Percent_Identity=35.4545454545455, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787229, Length=115, Percent_Identity=33.0434782608696, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=34.9593495934959, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=103, Percent_Identity=37.8640776699029, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 67981; Mature: 67981

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIIHEFKWICHMDLTSRILIVDDLPQERWMLGALLKSYDYELSYATNGHEALELAKKI
CHHHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHC
NPDLILLDVMMPGMDGFEVCRRIRKTPLIAEVPIMMVTALNEAEVRRKGVEIGADDFISK
CCCEEEEEECCCCCCHHHHHHHHHCCCCCHHCCHHHHHHCCHHHHHHCCCCCCCHHHHCC
PFDRAELQARVRNITRLNRHRRHRLHEERVIQEKLFNALPDGLMVLAENGTIQIVNPALR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECHHHH
RMLLYRDSTELMGRTLSELLAPDYRETFEGSFHRCLSDPSCMENNQLDFLRRDGSRMTAE
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEE
LTVTSLAGEGMRALQVILRDITDRIKTESRLRESEEQYRVLFMGSTQGILATDMETKRFT
EEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCEEE
FANPAVCRMLGYSEEELLRLGLADIHPRNFLDCAVIEFELQVRGEREISSGIPYLRKDGT
CCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHCCCCCEECCCE
VFYADTSGSKTLFKGRECHVSFLTEVTQRKLAEETLELTLNNLKKAFAGAIQVMVSAVEA
EEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDSYTAGHQRRTADLASTIAGEMGLSQEKIDAIRMAGSIHDIGKLFVPSEILCKPAKLFD
HCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCCEEE
IEFTLIKEHPRQGFEILKDVDFPWPLADIIYQHHERIDGSGYPRNLKGDDILVEARILSV
EEEEEEHHCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHH
ADTVEAMASHRPYRPGLGLDLALEEIEKNRGIFYDCVAVDACLRLFREKGYNFSLT
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKEIIHEFKWICHMDLTSRILIVDDLPQERWMLGALLKSYDYELSYATNGHEALELAKKI
CHHHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHC
NPDLILLDVMMPGMDGFEVCRRIRKTPLIAEVPIMMVTALNEAEVRRKGVEIGADDFISK
CCCEEEEEECCCCCCHHHHHHHHHCCCCCHHCCHHHHHHCCHHHHHHCCCCCCCHHHHCC
PFDRAELQARVRNITRLNRHRRHRLHEERVIQEKLFNALPDGLMVLAENGTIQIVNPALR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECHHHH
RMLLYRDSTELMGRTLSELLAPDYRETFEGSFHRCLSDPSCMENNQLDFLRRDGSRMTAE
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEE
LTVTSLAGEGMRALQVILRDITDRIKTESRLRESEEQYRVLFMGSTQGILATDMETKRFT
EEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCEEE
FANPAVCRMLGYSEEELLRLGLADIHPRNFLDCAVIEFELQVRGEREISSGIPYLRKDGT
CCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHCCCCCEECCCE
VFYADTSGSKTLFKGRECHVSFLTEVTQRKLAEETLELTLNNLKKAFAGAIQVMVSAVEA
EEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDSYTAGHQRRTADLASTIAGEMGLSQEKIDAIRMAGSIHDIGKLFVPSEILCKPAKLFD
HCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCCEEE
IEFTLIKEHPRQGFEILKDVDFPWPLADIIYQHHERIDGSGYPRNLKGDDILVEARILSV
EEEEEEHHCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHH
ADTVEAMASHRPYRPGLGLDLALEEIEKNRGIFYDCVAVDACLRLFREKGYNFSLT
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]