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The map label for this gene is gdhA [H]

Identifier: 85860318

GI number: 85860318

Start: 2498413

End: 2499699

Strand: Direct

Name: gdhA [H]

Synonym: SYN_01242

Alternate gene names: 85860318

Gene position: 2498413-2499699 (Clockwise)

Preceding gene: 85860311

Following gene: 85860319

Centisome position: 78.58

GC content: 52.21

Gene sequence:

>1287_bases
ATGGAAGACAAATCTTATAACCCGTTTGTACATGCCCAGGCGCAATTTGACAAGGTAGCTGCGATTATCGGTCTCGATGA
GGCAACCCGCGATTTGCTGAGACAGCCCATGAGGGAACACCATGTACTCATTCCCGTGCGCATGGACGATGGGACTACAA
AGATATTCAAGGGATTCCGTGTCATGCACAACGACGCGAGAGGACCGGCGAAAGGAGGCATTCGTTTTCACCCTCAGGAA
ACTGCAGATACCGTCCGCGCCCTTGCAATGTGGATGACCTGGAAGTGCGCGGTGGTTAATATCCCTCTCGGCGGAGGCAA
GGGAGGCGTTATATGCGACCCCCATAACCTGACCGAAACCGAGCAGGAACGGTTGTGCAGGGGATGGATAAGGAATCTCG
CGAAAGAAATGGGACCATGCATTGACGTGCCGGCCCCCGATGTCATGACGAACGCAAAGCATATGTTATGGATGCTCGAT
GAGTTTGAAGCCATTCACGGGGGTAAATATCCCGGGTTTATAACCGGAAAGCCGATAGAAATGGGTGGTTCGCTGGGAAG
GACGGAAGCCACCGGATTTGGAACCATCTATGTATTGCGGGAAGCGCTGAAAGACCTCGGAATCAAACCGGAAAACACGA
GCATCAGCATCCAGGGCTTCGGTAACGTATCGCAGTTCGCAGCCAGAAAGTTCAGTGAGCTCGGAGGAAAAATCGCCGCA
GTTTCCTGCTGGGACAATGCGGATAAAACCTCTCACACCTATTTCAAAAAAGACGGTCTGGATGTGGATATGCTTGCGTC
TATTACCAATACCTTCGGATGCATCAATAAGGAAAAGGCTGTTGCCGCAGGTTATGAAATTCTACCCGGTGATGCATGGG
TTGAACAGGATGTCGACATTCTCATGCCTGCCGCACTGGAAAACCAGATTACGTCGGCGAACGTGAACCGGATAAGCGAA
AAGGTCAAAGTCCTCCTTGAAGGCGCCAACGGGCCAACCGCTCCTGAGGCGGACGAAGTGATAAGGGCGCGCGGCATTTT
TCTTCTCCCCGATTTTCTTGCAAATGCCGGCGGTGTCACATGCAGCTATTTCGAGCAAGTGCAGTCAAACATGAACTACT
ACTGGGAAAAAGATGAAGTACTGGAGAGGCTCGATGCAAAAATGACATCGGCTTACCGGGCTGTTCAGGATCTTGCGCGG
AGCAAAGGCCTGTATATGCGGGACGCCGCCTATGTCATAGCTATCAACCGGGTGGCCCAGGCTGTCAAGATGAGGGGCTG
GGTATAG

Upstream 100 bases:

>100_bases
TTGATACCAGATTTTGCGTGCAGAGCAGTGCTTCAAACATCAGGAAACGGTGCGTTGATATTTTCGTTATTTAAATCTGA
AGACCAAGGAGAAGGAGGAT

Downstream 100 bases:

>100_bases
TAAGGAAAAATACTCGACACGAAGGTGGTTAATTGATGAAGCACGGTGAAGATCCAGGAGAAAGCTCGCTGGAACATTTG
AAAGAAAGAGCCAAAGAACT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MEDKSYNPFVHAQAQFDKVAAIIGLDEATRDLLRQPMREHHVLIPVRMDDGTTKIFKGFRVMHNDARGPAKGGIRFHPQE
TADTVRALAMWMTWKCAVVNIPLGGGKGGVICDPHNLTETEQERLCRGWIRNLAKEMGPCIDVPAPDVMTNAKHMLWMLD
EFEAIHGGKYPGFITGKPIEMGGSLGRTEATGFGTIYVLREALKDLGIKPENTSISIQGFGNVSQFAARKFSELGGKIAA
VSCWDNADKTSHTYFKKDGLDVDMLASITNTFGCINKEKAVAAGYEILPGDAWVEQDVDILMPAALENQITSANVNRISE
KVKVLLEGANGPTAPEADEVIRARGIFLLPDFLANAGGVTCSYFEQVQSNMNYYWEKDEVLERLDAKMTSAYRAVQDLAR
SKGLYMRDAAYVIAINRVAQAVKMRGWV

Sequences:

>Translated_428_residues
MEDKSYNPFVHAQAQFDKVAAIIGLDEATRDLLRQPMREHHVLIPVRMDDGTTKIFKGFRVMHNDARGPAKGGIRFHPQE
TADTVRALAMWMTWKCAVVNIPLGGGKGGVICDPHNLTETEQERLCRGWIRNLAKEMGPCIDVPAPDVMTNAKHMLWMLD
EFEAIHGGKYPGFITGKPIEMGGSLGRTEATGFGTIYVLREALKDLGIKPENTSISIQGFGNVSQFAARKFSELGGKIAA
VSCWDNADKTSHTYFKKDGLDVDMLASITNTFGCINKEKAVAAGYEILPGDAWVEQDVDILMPAALENQITSANVNRISE
KVKVLLEGANGPTAPEADEVIRARGIFLLPDFLANAGGVTCSYFEQVQSNMNYYWEKDEVLERLDAKMTSAYRAVQDLAR
SKGLYMRDAAYVIAINRVAQAVKMRGWV
>Mature_428_residues
MEDKSYNPFVHAQAQFDKVAAIIGLDEATRDLLRQPMREHHVLIPVRMDDGTTKIFKGFRVMHNDARGPAKGGIRFHPQE
TADTVRALAMWMTWKCAVVNIPLGGGKGGVICDPHNLTETEQERLCRGWIRNLAKEMGPCIDVPAPDVMTNAKHMLWMLD
EFEAIHGGKYPGFITGKPIEMGGSLGRTEATGFGTIYVLREALKDLGIKPENTSISIQGFGNVSQFAARKFSELGGKIAA
VSCWDNADKTSHTYFKKDGLDVDMLASITNTFGCINKEKAVAAGYEILPGDAWVEQDVDILMPAALENQITSANVNRISE
KVKVLLEGANGPTAPEADEVIRARGIFLLPDFLANAGGVTCSYFEQVQSNMNYYWEKDEVLERLDAKMTSAYRAVQDLAR
SKGLYMRDAAYVIAINRVAQAVKMRGWV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=399, Percent_Identity=37.34335839599, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI4885281, Length=360, Percent_Identity=39.1666666666667, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1788059, Length=350, Percent_Identity=32.5714285714286, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17544676, Length=347, Percent_Identity=35.7348703170029, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324951, Length=399, Percent_Identity=28.0701754385965, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319256, Length=416, Percent_Identity=27.4038461538462, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24649283, Length=384, Percent_Identity=36.4583333333333, Blast_Score=212, Evalue=4e-55,
Organism=Drosophila melanogaster, GI45549226, Length=398, Percent_Identity=33.6683417085427, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI45553475, Length=357, Percent_Identity=36.1344537815126, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24649460, Length=336, Percent_Identity=33.3333333333333, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 47266; Mature: 47266

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKSYNPFVHAQAQFDKVAAIIGLDEATRDLLRQPMREHHVLIPVRMDDGTTKIFKGFR
CCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHH
VMHNDARGPAKGGIRFHPQETADTVRALAMWMTWKCAVVNIPLGGGKGGVICDPHNLTET
HHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCEEECCCCCCHH
EQERLCRGWIRNLAKEMGPCIDVPAPDVMTNAKHMLWMLDEFEAIHGGKYPGFITGKPIE
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCEECCCCHH
MGGSLGRTEATGFGTIYVLREALKDLGIKPENTSISIQGFGNVSQFAARKFSELGGKIAA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEE
VSCWDNADKTSHTYFKKDGLDVDMLASITNTFGCINKEKAVAAGYEILPGDAWVEQDVDI
EEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEECCCCCHHHCCHHH
LMPAALENQITSANVNRISEKVKVLLEGANGPTAPEADEVIRARGIFLLPDFLANAGGVT
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCEEEHHHHCCCCCCH
CSYFEQVQSNMNYYWEKDEVLERLDAKMTSAYRAVQDLARSKGLYMRDAAYVIAINRVAQ
HHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHEEHHHHHHH
AVKMRGWV
HHHHCCCC
>Mature Secondary Structure
MEDKSYNPFVHAQAQFDKVAAIIGLDEATRDLLRQPMREHHVLIPVRMDDGTTKIFKGFR
CCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHH
VMHNDARGPAKGGIRFHPQETADTVRALAMWMTWKCAVVNIPLGGGKGGVICDPHNLTET
HHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCEEECCCCCCHH
EQERLCRGWIRNLAKEMGPCIDVPAPDVMTNAKHMLWMLDEFEAIHGGKYPGFITGKPIE
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCEECCCCHH
MGGSLGRTEATGFGTIYVLREALKDLGIKPENTSISIQGFGNVSQFAARKFSELGGKIAA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEE
VSCWDNADKTSHTYFKKDGLDVDMLASITNTFGCINKEKAVAAGYEILPGDAWVEQDVDI
EEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHCCCEECCCCCHHHCCHHH
LMPAALENQITSANVNRISEKVKVLLEGANGPTAPEADEVIRARGIFLLPDFLANAGGVT
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCEEEHHHHCCCCCCH
CSYFEQVQSNMNYYWEKDEVLERLDAKMTSAYRAVQDLARSKGLYMRDAAYVIAINRVAQ
HHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHEEHHHHHHH
AVKMRGWV
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA