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The map label for this gene is fdoI [H]

Identifier: 85860213

GI number: 85860213

Start: 2413113

End: 2413781

Strand: Direct

Name: fdoI [H]

Synonym: SYN_00605

Alternate gene names: 85860213

Gene position: 2413113-2413781 (Clockwise)

Preceding gene: 85860212

Following gene: 85860216

Centisome position: 75.9

GC content: 50.97

Gene sequence:

>669_bases
ATGAGAAAAGGATTGATTCAAGCGACGGACTCCTTTGAGAGGATCGTGCACTGGTGTCTGGCGATCTCCTGCCTGATCCT
GTGCATTACGGGCCTGGGAATGATGTTCCATTCCCTTAACTTTATCGGTACTCCCTTTGGCGGTTTGAAGTCTTTGAAGT
ACCTTCACAATTTCACGGCGCTCTTTTTCATCCTGTCCCTCTATTTCACCATCCGCATGTGGTGGAAGGAAGCGGGAATT
TTCGTTTTTCCTGAAGATCTGGACTGGATCAAGAGTGCGGGCGGATATCTGTGGCATGTGGACAAGGTTCCAGAGGTGGG
CAAGTACAATCCGGGACAGAAGATGTTCTTCCTGGTTGTAGCAGGCGGCGGAGCGGCCATGGTCCTGTCAGGATTGATTA
TGCTGTTCCCGTTGAATATCCCGGCCGCCCTCGTGAGACTGATGTATCTGATCCACGCCGCTGGATTTGTGGCGATTTTC
GCCTTCTTCTTCATCCACCTTTATCTGGGAACGATCGGTTCTCCCGGTTCATTGCCGGCTATGCTGAACGGCTGGGTAAC
ACGAGCCTGGCTGAAGAAACAGCATCCCAAGTGGCTCAAGGAAATGGAAGAGGAAGGAAAACTGGTGGTGTTTGGAGAAG
AAAAGACAAACGCTGGTCATGGCCATTAA

Upstream 100 bases:

>100_bases
CCGGCGGCGCCCTTGCCGGTTCATTCTTGCATTACATTATCCACGGGCCCAAACTTCCAGATGAAGAACAGGGTCAAGAA
CCGAAGGGAGGGGAATAACC

Downstream 100 bases:

>100_bases
TCAGTCATAGATCCTGATTTTCCCATTGAATGAAAAAGGCCCTCCTTTGGAGGGTCTTTTTTTTTGCGTAATTGCATGTG
GATTTAAATAAAAACCCTTC

Product: formate dehydrogenase cytochromesubunit B

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MRKGLIQATDSFERIVHWCLAISCLILCITGLGMMFHSLNFIGTPFGGLKSLKYLHNFTALFFILSLYFTIRMWWKEAGI
FVFPEDLDWIKSAGGYLWHVDKVPEVGKYNPGQKMFFLVVAGGGAAMVLSGLIMLFPLNIPAALVRLMYLIHAAGFVAIF
AFFFIHLYLGTIGSPGSLPAMLNGWVTRAWLKKQHPKWLKEMEEEGKLVVFGEEKTNAGHGH

Sequences:

>Translated_222_residues
MRKGLIQATDSFERIVHWCLAISCLILCITGLGMMFHSLNFIGTPFGGLKSLKYLHNFTALFFILSLYFTIRMWWKEAGI
FVFPEDLDWIKSAGGYLWHVDKVPEVGKYNPGQKMFFLVVAGGGAAMVLSGLIMLFPLNIPAALVRLMYLIHAAGFVAIF
AFFFIHLYLGTIGSPGSLPAMLNGWVTRAWLKKQHPKWLKEMEEEGKLVVFGEEKTNAGHGH
>Mature_222_residues
MRKGLIQATDSFERIVHWCLAISCLILCITGLGMMFHSLNFIGTPFGGLKSLKYLHNFTALFFILSLYFTIRMWWKEAGI
FVFPEDLDWIKSAGGYLWHVDKVPEVGKYNPGQKMFFLVVAGGGAAMVLSGLIMLFPLNIPAALVRLMYLIHAAGFVAIF
AFFFIHLYLGTIGSPGSLPAMLNGWVTRAWLKKQHPKWLKEMEEEGKLVVFGEEKTNAGHGH

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=209, Percent_Identity=27.7511961722488, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1787750, Length=209, Percent_Identity=28.7081339712919, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24960; Mature: 24960

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGLIQATDSFERIVHWCLAISCLILCITGLGMMFHSLNFIGTPFGGLKSLKYLHNFTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LFFILSLYFTIRMWWKEAGIFVFPEDLDWIKSAGGYLWHVDKVPEVGKYNPGQKMFFLVV
HHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEE
AGGGAAMVLSGLIMLFPLNIPAALVRLMYLIHAAGFVAIFAFFFIHLYLGTIGSPGSLPA
ECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MLNGWVTRAWLKKQHPKWLKEMEEEGKLVVFGEEKTNAGHGH
HHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MRKGLIQATDSFERIVHWCLAISCLILCITGLGMMFHSLNFIGTPFGGLKSLKYLHNFTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LFFILSLYFTIRMWWKEAGIFVFPEDLDWIKSAGGYLWHVDKVPEVGKYNPGQKMFFLVV
HHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEE
AGGGAAMVLSGLIMLFPLNIPAALVRLMYLIHAAGFVAIFAFFFIHLYLGTIGSPGSLPA
ECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MLNGWVTRAWLKKQHPKWLKEMEEEGKLVVFGEEKTNAGHGH
HHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]