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The map label for this gene is fdoH [H]

Identifier: 85860212

GI number: 85860212

Start: 2412319

End: 2413110

Strand: Direct

Name: fdoH [H]

Synonym: SYN_00604

Alternate gene names: 85860212

Gene position: 2412319-2413110 (Clockwise)

Preceding gene: 85860211

Following gene: 85860213

Centisome position: 75.88

GC content: 52.15

Gene sequence:

>792_bases
ATGGCAGAATATATAAAACTTATTGATGTTTCCAAATGTACCGGCTGCCGGGGTTGTCAGCTGGCCTGCAAACAGTGGAA
TCAGCAGAAGGCGAAACAGACCGTGAATTATGGAACCTACCAGAATCCTCCAGACCTTCAGGCCAACACATGGACTCTGA
TCCGGTTTCAGGAAGTCGAGGAAAAGGACACGGTCAAGTGGCTCTTTCGGAAAGACGGATGCATGCATTGCACGAACGCA
GCCTGTGTCAAGGTTTGCCCCAGCGGCGCGCTTCACTATACAGAGGTGGGAACGGTTGGTTTGAACCATGAGCTTTGCAT
CGGCTGCAAGGAATGTGTCTCGGCCTGTCCCTTCAACATTCCCCGGTATGACCGGGAGACAGACAAGGTTTACAAATGCG
ACCTCTGCCTGAGCCGCATCAGGGCCGATCTTATTCCCGCCTGCGCAAAGGCATGCCCAACGGGAGCCATTACCTTCGGT
GAGAAGTCCGCTATGATCAAGAAGGCTCATGAGCGGGTCAAGGAATTGGGCGGGGATGCCAATGTCTATGGGGACAAGTT
TGTCGGCGGCACCCATGTCATGTATGTTCTGGATGAAAAACCTTCTGTTTATGAGAAATTGCCGAAAAATCCTTCAGTGC
CAATCACCGTCACGCTTTGGAAAGACATTATGAAGCCTCTCAGCCTTCTGGCGGCCGGCGGCGCCCTTGCCGGTTCATTC
TTGCATTACATTATCCACGGGCCCAAACTTCCAGATGAAGAACAGGGTCAAGAACCGAAGGGAGGGGAATAA

Upstream 100 bases:

>100_bases
CCAATCTCCTGGTGCCGACGATCGGGGACGCCAACACGACGATCCCGGAAAGCAAGGCCTTCATGGTTAATGTGGTCAAG
AAGTAAGGGGGTAAACAAAA

Downstream 100 bases:

>100_bases
CCATGAGAAAAGGATTGATTCAAGCGACGGACTCCTTTGAGAGGATCGTGCACTGGTGTCTGGCGATCTCCTGCCTGATC
CTGTGCATTACGGGCCTGGG

Product: formate dehydrogenase iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MAEYIKLIDVSKCTGCRGCQLACKQWNQQKAKQTVNYGTYQNPPDLQANTWTLIRFQEVEEKDTVKWLFRKDGCMHCTNA
ACVKVCPSGALHYTEVGTVGLNHELCIGCKECVSACPFNIPRYDRETDKVYKCDLCLSRIRADLIPACAKACPTGAITFG
EKSAMIKKAHERVKELGGDANVYGDKFVGGTHVMYVLDEKPSVYEKLPKNPSVPITVTLWKDIMKPLSLLAAGGALAGSF
LHYIIHGPKLPDEEQGQEPKGGE

Sequences:

>Translated_263_residues
MAEYIKLIDVSKCTGCRGCQLACKQWNQQKAKQTVNYGTYQNPPDLQANTWTLIRFQEVEEKDTVKWLFRKDGCMHCTNA
ACVKVCPSGALHYTEVGTVGLNHELCIGCKECVSACPFNIPRYDRETDKVYKCDLCLSRIRADLIPACAKACPTGAITFG
EKSAMIKKAHERVKELGGDANVYGDKFVGGTHVMYVLDEKPSVYEKLPKNPSVPITVTLWKDIMKPLSLLAAGGALAGSF
LHYIIHGPKLPDEEQGQEPKGGE
>Mature_262_residues
AEYIKLIDVSKCTGCRGCQLACKQWNQQKAKQTVNYGTYQNPPDLQANTWTLIRFQEVEEKDTVKWLFRKDGCMHCTNAA
CVKVCPSGALHYTEVGTVGLNHELCIGCKECVSACPFNIPRYDRETDKVYKCDLCLSRIRADLIPACAKACPTGAITFGE
KSAMIKKAHERVKELGGDANVYGDKFVGGTHVMYVLDEKPSVYEKLPKNPSVPITVTLWKDIMKPLSLLAAGGALAGSFL
HYIIHGPKLPDEEQGQEPKGGE

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=262, Percent_Identity=47.7099236641221, Blast_Score=257, Evalue=6e-70,
Organism=Escherichia coli, GI1787749, Length=263, Percent_Identity=46.7680608365019, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1787872, Length=166, Percent_Identity=34.3373493975904, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1789370, Length=268, Percent_Identity=28.7313432835821, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787122, Length=172, Percent_Identity=33.1395348837209, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI2367345, Length=205, Percent_Identity=32.1951219512195, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI226510944, Length=163, Percent_Identity=30.0613496932515, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87082179, Length=177, Percent_Identity=29.9435028248588, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787963, Length=152, Percent_Identity=28.9473684210526, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788811, Length=179, Percent_Identity=28.4916201117318, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082180, Length=171, Percent_Identity=29.2397660818713, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI2367245, Length=159, Percent_Identity=31.4465408805031, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789067, Length=190, Percent_Identity=30, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787478, Length=145, Percent_Identity=28.2758620689655, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 29063; Mature: 28932

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
6.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEYIKLIDVSKCTGCRGCQLACKQWNQQKAKQTVNYGTYQNPPDLQANTWTLIRFQEVE
CCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCC
EKDTVKWLFRKDGCMHCTNAACVKVCPSGALHYTEVGTVGLNHELCIGCKECVSACPFNI
CHHHHHHHHHCCCCCCCCCCHHEEECCCCCEEEEEECCCCCCCHHHHCHHHHHHHCCCCC
PRYDRETDKVYKCDLCLSRIRADLIPACAKACPTGAITFGEKSAMIKKAHERVKELGGDA
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCCCC
NVYGDKFVGGTHVMYVLDEKPSVYEKLPKNPSVPITVTLWKDIMKPLSLLAAGGALAGSF
CCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHH
LHYIIHGPKLPDEEQGQEPKGGE
HHHHHCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure 
AEYIKLIDVSKCTGCRGCQLACKQWNQQKAKQTVNYGTYQNPPDLQANTWTLIRFQEVE
CCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCC
EKDTVKWLFRKDGCMHCTNAACVKVCPSGALHYTEVGTVGLNHELCIGCKECVSACPFNI
CHHHHHHHHHCCCCCCCCCCHHEEECCCCCEEEEEECCCCCCCHHHHCHHHHHHHCCCCC
PRYDRETDKVYKCDLCLSRIRADLIPACAKACPTGAITFGEKSAMIKKAHERVKELGGDA
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCCCC
NVYGDKFVGGTHVMYVLDEKPSVYEKLPKNPSVPITVTLWKDIMKPLSLLAAGGALAGSF
CCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHH
LHYIIHGPKLPDEEQGQEPKGGE
HHHHHCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]