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The map label for this gene is appA [H]

Identifier: 85860216

GI number: 85860216

Start: 2416303

End: 2418081

Strand: Direct

Name: appA [H]

Synonym: SYN_00608

Alternate gene names: 85860216

Gene position: 2416303-2418081 (Clockwise)

Preceding gene: 85860213

Following gene: 85860219

Centisome position: 76.0

GC content: 57.28

Gene sequence:

>1779_bases
ATGAGACACACCGTCAATACGGCAACCTGTTCTTTCGGATCGCTTCCGGATGAGGATAGCCGCAGGGCAAAGGAAATGAC
GGAGTCTGTTGAATTCCATTGCAAAAAAAACGGCTCTGAGATACTTTCCCCATCCATGAAATCCTCTTTTTTCCGCTTCC
TTTTGATCGGCATTCTTCTCGTCGGCGGGTTTACCGGATGCGGCGAGACGGACAGCAGTCCCCAGCAGGCCCGACAGCAG
GCGCCAGGCACCCCGGCTTATGGCGACATCATCGTGGAAGGTTCCATCGGGGACGCCAGCAACCTGATCCCCCTGCTCTC
CTCCGATTCAACTTCGCATGAAATCGCCGGTCTGGTTTACAACGGCCTTGTCAAATACGACAAAAATCTGAAAATTACCG
GTGACCTTGCCGAATCCTGGGGAATTTCCCCCGATGGGCTGATCATCACCTTCCATCTCCGTCACAATGTCCGCTGGCAC
GACGGTCATCCCTTCACCGCCGAGGATGTCCTTTTCACCTACCAGCTTACCATCGATCCCAAAACGCCCACGGCCTACGC
CGGAGACTTTCTCAAGGTAAAGAAGGCGGAAGTGCTCGACGCCCACACCTTCCGGGTCACCTACGACAAGCCCTTCGCCC
CCGCCCTGATGAGCTGGGGCAGCGCCATTATGCCCAAGCATCTCCTGGCCGGTCAGGACATCACAAGGACGCCCCTCGCC
CGGAAACCCGTCGGAACGGGCCCTTACAAATTCAAGGAATGGGTGGCCGGACAGAAGATCGTGCTGGTCTCCAATCCGGA
CTACTATGAAGGCAGACCCTACATCGACGGCTACATCCTGCGGATTATCCCCGACATGGCCACCATGTTCCTGGAACTGC
GCGCCGGCGGCATCGACCGGATGGGCCTGACGCCGCTCCAGTATACGCGGCAGACGGAAAACCCCCTGTTCCGCAAGAAT
TACAACAAGTTCCGTTATCTCTCCTTTGCCTACACCTTTGTCGGCTACAACCTCGGAAATCCCCTTTTCGCTGACCGCCG
GGTTCGTCAGGCCCTGGCTTATGCCATCGACAAGGAGGAAATCATCCAGGGTGTTCTGCTGGGCCTGGGGAAACCTGCCA
CCGGCCCTTTTAAACCGGGGACCTGGGCCTATAATCCGAATGTCCCGACCTTCCCCCACGATCCCGGAAAGGCCCGCGCC
CTGCTGGCCGAAGCAGGCTGGCTGGATCGCAACGGCGACGGCATCCTGGACAGAAACGGCCAACCCTTCGCCTTTGAACT
GCTGGTCAACCAGGGCAATGAGGTCCGGGCCAAGTGCGCTGAAATCATCCAGCGCCGTCTGGCCGAGATCGGCATTTCCG
TCAAGATCCGGGTGGTGGAATGGGCCGCCTTTGTCAACGACTTCATCAACAAACGGCGCTTTGACGCCACCATTCTGGGA
TGGACGATTCCTCTGGACCCCGATCTCTATGACGTCTGGCATTCCAGCAAGACCGGTCCCCAGGAACTGAATTTCACGTC
ATTCAAGAATGAGGAAGTGGACCGCCTCCTGGAAAAGGGACGCGGCACCTTCGATCTGAACGAACGCAAAAGGTGCTACG
ACCGGATACAGGAAATCCTGGCGGAAGAACAGCCTTACACCTTTCTTTATGTTCCTGATGCCCTGCCCATCGTTCAGGCC
CGTTTCCGGGGCATTGAACTGGCACCCCTGGGCATCGGCCACAATTTCATCAAGTGGTACGTCCCTGCTCCGGAACAGCG
ATTCATCATGACCCGGTAA

Upstream 100 bases:

>100_bases
CTCGAAGTGCTGTGGCGATAGCATGATTAATACATAACTGTCAAGACAAATAAGGATAAATTCCGCCTGTCACAGGTTCG
GATTCAGCTCATCCAGAGGG

Downstream 100 bases:

>100_bases
GGCCGGTCAAGATCACACCGCAGGGTTTCCCATCTCCATTTTCAGATCGGTCAGGGCCTTGATCTGATCCCTCAAGCGCG
CGGCTTTTTCGAACTCCAGT

Product: oligopeptide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MRHTVNTATCSFGSLPDEDSRRAKEMTESVEFHCKKNGSEILSPSMKSSFFRFLLIGILLVGGFTGCGETDSSPQQARQQ
APGTPAYGDIIVEGSIGDASNLIPLLSSDSTSHEIAGLVYNGLVKYDKNLKITGDLAESWGISPDGLIITFHLRHNVRWH
DGHPFTAEDVLFTYQLTIDPKTPTAYAGDFLKVKKAEVLDAHTFRVTYDKPFAPALMSWGSAIMPKHLLAGQDITRTPLA
RKPVGTGPYKFKEWVAGQKIVLVSNPDYYEGRPYIDGYILRIIPDMATMFLELRAGGIDRMGLTPLQYTRQTENPLFRKN
YNKFRYLSFAYTFVGYNLGNPLFADRRVRQALAYAIDKEEIIQGVLLGLGKPATGPFKPGTWAYNPNVPTFPHDPGKARA
LLAEAGWLDRNGDGILDRNGQPFAFELLVNQGNEVRAKCAEIIQRRLAEIGISVKIRVVEWAAFVNDFINKRRFDATILG
WTIPLDPDLYDVWHSSKTGPQELNFTSFKNEEVDRLLEKGRGTFDLNERKRCYDRIQEILAEEQPYTFLYVPDALPIVQA
RFRGIELAPLGIGHNFIKWYVPAPEQRFIMTR

Sequences:

>Translated_592_residues
MRHTVNTATCSFGSLPDEDSRRAKEMTESVEFHCKKNGSEILSPSMKSSFFRFLLIGILLVGGFTGCGETDSSPQQARQQ
APGTPAYGDIIVEGSIGDASNLIPLLSSDSTSHEIAGLVYNGLVKYDKNLKITGDLAESWGISPDGLIITFHLRHNVRWH
DGHPFTAEDVLFTYQLTIDPKTPTAYAGDFLKVKKAEVLDAHTFRVTYDKPFAPALMSWGSAIMPKHLLAGQDITRTPLA
RKPVGTGPYKFKEWVAGQKIVLVSNPDYYEGRPYIDGYILRIIPDMATMFLELRAGGIDRMGLTPLQYTRQTENPLFRKN
YNKFRYLSFAYTFVGYNLGNPLFADRRVRQALAYAIDKEEIIQGVLLGLGKPATGPFKPGTWAYNPNVPTFPHDPGKARA
LLAEAGWLDRNGDGILDRNGQPFAFELLVNQGNEVRAKCAEIIQRRLAEIGISVKIRVVEWAAFVNDFINKRRFDATILG
WTIPLDPDLYDVWHSSKTGPQELNFTSFKNEEVDRLLEKGRGTFDLNERKRCYDRIQEILAEEQPYTFLYVPDALPIVQA
RFRGIELAPLGIGHNFIKWYVPAPEQRFIMTR
>Mature_592_residues
MRHTVNTATCSFGSLPDEDSRRAKEMTESVEFHCKKNGSEILSPSMKSSFFRFLLIGILLVGGFTGCGETDSSPQQARQQ
APGTPAYGDIIVEGSIGDASNLIPLLSSDSTSHEIAGLVYNGLVKYDKNLKITGDLAESWGISPDGLIITFHLRHNVRWH
DGHPFTAEDVLFTYQLTIDPKTPTAYAGDFLKVKKAEVLDAHTFRVTYDKPFAPALMSWGSAIMPKHLLAGQDITRTPLA
RKPVGTGPYKFKEWVAGQKIVLVSNPDYYEGRPYIDGYILRIIPDMATMFLELRAGGIDRMGLTPLQYTRQTENPLFRKN
YNKFRYLSFAYTFVGYNLGNPLFADRRVRQALAYAIDKEEIIQGVLLGLGKPATGPFKPGTWAYNPNVPTFPHDPGKARA
LLAEAGWLDRNGDGILDRNGQPFAFELLVNQGNEVRAKCAEIIQRRLAEIGISVKIRVVEWAAFVNDFINKRRFDATILG
WTIPLDPDLYDVWHSSKTGPQELNFTSFKNEEVDRLLEKGRGTFDLNERKRCYDRIQEILAEEQPYTFLYVPDALPIVQA
RFRGIELAPLGIGHNFIKWYVPAPEQRFIMTR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=495, Percent_Identity=28.4848484848485, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI1789966, Length=516, Percent_Identity=25.968992248062, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1787052, Length=503, Percent_Identity=26.441351888668, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1789887, Length=472, Percent_Identity=26.4830508474576, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1789397, Length=487, Percent_Identity=26.2833675564682, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787551, Length=557, Percent_Identity=24.9551166965889, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081878, Length=460, Percent_Identity=24.1304347826087, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787495, Length=475, Percent_Identity=24, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082063, Length=394, Percent_Identity=25.3807106598985, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66641; Mature: 66641

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHTVNTATCSFGSLPDEDSRRAKEMTESVEFHCKKNGSEILSPSMKSSFFRFLLIGILL
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHH
VGGFTGCGETDSSPQQARQQAPGTPAYGDIIVEGSIGDASNLIPLLSSDSTSHEIAGLVY
HCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHH
NGLVKYDKNLKITGDLAESWGISPDGLIITFHLRHNVRWHDGHPFTAEDVLFTYQLTIDP
HHHHEECCCEEEEECHHHHCCCCCCCEEEEEEEECCCEECCCCCCCCCCEEEEEEEEECC
KTPTAYAGDFLKVKKAEVLDAHTFRVTYDKPFAPALMSWGSAIMPKHLLAGQDITRTPLA
CCCCCCCCCCEEEEHHHHCCCEEEEEEECCCCHHHHHHCCCHHCCHHHHCCCCCCCCCCC
RKPVGTGPYKFKEWVAGQKIVLVSNPDYYEGRPYIDGYILRIIPDMATMFLELRAGGIDR
CCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCC
MGLTPLQYTRQTENPLFRKNYNKFRYLSFAYTFVGYNLGNPLFADRRVRQALAYAIDKEE
CCCCHHHHHCCCCCCHHHCCCCCEEEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHCCHHH
IIQGVLLGLGKPATGPFKPGTWAYNPNVPTFPHDPGKARALLAEAGWLDRNGDGILDRNG
HHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHEEEHHCCCCCCCCCEEECCCC
QPFAFELLVNQGNEVRAKCAEIIQRRLAEIGISVKIRVVEWAAFVNDFINKRRFDATILG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCCCEEEEE
WTIPLDPDLYDVWHSSKTGPQELNFTSFKNEEVDRLLEKGRGTFDLNERKRCYDRIQEIL
EEECCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AEEQPYTFLYVPDALPIVQARFRGIELAPLGIGHNFIKWYVPAPEQRFIMTR
HCCCCEEEEECCCCCHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCEECCC
>Mature Secondary Structure
MRHTVNTATCSFGSLPDEDSRRAKEMTESVEFHCKKNGSEILSPSMKSSFFRFLLIGILL
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHH
VGGFTGCGETDSSPQQARQQAPGTPAYGDIIVEGSIGDASNLIPLLSSDSTSHEIAGLVY
HCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHH
NGLVKYDKNLKITGDLAESWGISPDGLIITFHLRHNVRWHDGHPFTAEDVLFTYQLTIDP
HHHHEECCCEEEEECHHHHCCCCCCCEEEEEEEECCCEECCCCCCCCCCEEEEEEEEECC
KTPTAYAGDFLKVKKAEVLDAHTFRVTYDKPFAPALMSWGSAIMPKHLLAGQDITRTPLA
CCCCCCCCCCEEEEHHHHCCCEEEEEEECCCCHHHHHHCCCHHCCHHHHCCCCCCCCCCC
RKPVGTGPYKFKEWVAGQKIVLVSNPDYYEGRPYIDGYILRIIPDMATMFLELRAGGIDR
CCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCC
MGLTPLQYTRQTENPLFRKNYNKFRYLSFAYTFVGYNLGNPLFADRRVRQALAYAIDKEE
CCCCHHHHHCCCCCCHHHCCCCCEEEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHCCHHH
IIQGVLLGLGKPATGPFKPGTWAYNPNVPTFPHDPGKARALLAEAGWLDRNGDGILDRNG
HHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHEEEHHCCCCCCCCCEEECCCC
QPFAFELLVNQGNEVRAKCAEIIQRRLAEIGISVKIRVVEWAAFVNDFINKRRFDATILG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCCCEEEEE
WTIPLDPDLYDVWHSSKTGPQELNFTSFKNEEVDRLLEKGRGTFDLNERKRCYDRIQEIL
EEECCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AEEQPYTFLYVPDALPIVQARFRGIELAPLGIGHNFIKWYVPAPEQRFIMTR
HCCCCEEEEECCCCCHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]