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The map label for this gene is hupE [H]

Identifier: 85860209

GI number: 85860209

Start: 2407735

End: 2408745

Strand: Direct

Name: hupE [H]

Synonym: SYN_00601

Alternate gene names: 85860209

Gene position: 2407735-2408745 (Clockwise)

Preceding gene: 85860208

Following gene: 85860210

Centisome position: 75.73

GC content: 58.36

Gene sequence:

>1011_bases
ATGGATCATGATCGAATTCTTCTGGAACATGGTGGGGGAGGATTGCTCAGCCACGAACTCGTGACTGGGATTTTTCTTCC
CCTTTTCAGGAATGCCTGTCTCGAACGACTGGAAGACAGCGCCGTTATCACCCTGGGCGACAGGAAGCTCTGCTTCACGA
CGGATTCCTACGTCATCGATCCGCTCTTCTTCCCCGGCGGCGATATCGGCTCCCTGGCGGTCCATGGAACCATTAACGAT
CTGTCCGTCTGCGGCGGAAAACCGTTGATGATGAGCGCCGGCTTCATTCTCGAAGAAGGGTTTCCCATGGAGGATCTCCG
CCGTATTTCCCGCTCCATGGCGGAAGCGGCTCAAAAGGCCGGTGTTTCCATCGTGACTGGCGATACAAAGGTTGTGGCCC
GCGGGGCCGCCGACGGTCTCTTCATCAACACCTCCGGCGTCGGCATCATCGAGTACCCTTCGCCCCTTTCTGTGGCAAGC
ATCGAACCCGGCGATGCGGTTATCGTGAGCGGGACGATCGGCGACCACGGAGCCACGGTCCTCAGCAAACGCCGGGAACT
GGGCTTGATATCAGAAATCCGCTCCGATTCGGCGCCGCTTAACGGCCTCATCGGGTCGATCCTGGAAGCCTCTCCGAATG
TCCACTGCATGCGGGATCCCACCCGTGGGGGACTCGGGGCGATCCTTGCCGAAATTGCCGCACAATCCGGTTTCCGCATC
ACGATTGAGGAAACGAAACTGCCTGTCCGGGAAGAAGTCAGGGGGATCTGCGAAATATTGGGCTTCGACCCTCTTTTTCT
AGCCAATGAAGGCAAGGTGGCCGTTTTCTGTTCCCCTGCCGATGCCTGCCTGATCCTTGACAAAATGAAAAACCATGAGT
ACGGCCGCGACGCCGCCCTCATCGGCTTTGTCGGGAAGAGGAACGGCGAAGGGCTCATTCTGAACACATCCGTCGGTGGC
TCCCGCGTTGTGGACCTGCCATTGGGAGAGCTGGTCCCGAGGATCTGCTGA

Upstream 100 bases:

>100_bases
TGCGGCCGCCGCACGCATGGAAAGGCAAGAGGCAGGACGGTAAAAACAGGAAACGGCCATTGAATACAAAAACGCAATAA
AGGAAAAAGGTTATAGCCCG

Downstream 100 bases:

>100_bases
TTCTCCCTTTTGACAACAAAATCGGTACTATCCTTTCATTCAGAGAAGCGTCTGCATGGGACGGCGTCTGGCCGCCCCAT
GATCGCCCTCTTCAGCACCC

Product: hypE-like hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MDHDRILLEHGGGGLLSHELVTGIFLPLFRNACLERLEDSAVITLGDRKLCFTTDSYVIDPLFFPGGDIGSLAVHGTIND
LSVCGGKPLMMSAGFILEEGFPMEDLRRISRSMAEAAQKAGVSIVTGDTKVVARGAADGLFINTSGVGIIEYPSPLSVAS
IEPGDAVIVSGTIGDHGATVLSKRRELGLISEIRSDSAPLNGLIGSILEASPNVHCMRDPTRGGLGAILAEIAAQSGFRI
TIEETKLPVREEVRGICEILGFDPLFLANEGKVAVFCSPADACLILDKMKNHEYGRDAALIGFVGKRNGEGLILNTSVGG
SRVVDLPLGELVPRIC

Sequences:

>Translated_336_residues
MDHDRILLEHGGGGLLSHELVTGIFLPLFRNACLERLEDSAVITLGDRKLCFTTDSYVIDPLFFPGGDIGSLAVHGTIND
LSVCGGKPLMMSAGFILEEGFPMEDLRRISRSMAEAAQKAGVSIVTGDTKVVARGAADGLFINTSGVGIIEYPSPLSVAS
IEPGDAVIVSGTIGDHGATVLSKRRELGLISEIRSDSAPLNGLIGSILEASPNVHCMRDPTRGGLGAILAEIAAQSGFRI
TIEETKLPVREEVRGICEILGFDPLFLANEGKVAVFCSPADACLILDKMKNHEYGRDAALIGFVGKRNGEGLILNTSVGG
SRVVDLPLGELVPRIC
>Mature_336_residues
MDHDRILLEHGGGGLLSHELVTGIFLPLFRNACLERLEDSAVITLGDRKLCFTTDSYVIDPLFFPGGDIGSLAVHGTIND
LSVCGGKPLMMSAGFILEEGFPMEDLRRISRSMAEAAQKAGVSIVTGDTKVVARGAADGLFINTSGVGIIEYPSPLSVAS
IEPGDAVIVSGTIGDHGATVLSKRRELGLISEIRSDSAPLNGLIGSILEASPNVHCMRDPTRGGLGAILAEIAAQSGFRI
TIEETKLPVREEVRGICEILGFDPLFLANEGKVAVFCSPADACLILDKMKNHEYGRDAALIGFVGKRNGEGLILNTSVGG
SRVVDLPLGELVPRIC

Specific function: May be involved in the maturation of the nifE hydrogenase [H]

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=339, Percent_Identity=48.9675516224189, Blast_Score=293, Evalue=9e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 35462; Mature: 35462

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHDRILLEHGGGGLLSHELVTGIFLPLFRNACLERLEDSAVITLGDRKLCFTTDSYVID
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCEEEC
PLFFPGGDIGSLAVHGTINDLSVCGGKPLMMSAGFILEEGFPMEDLRRISRSMAEAAQKA
EEECCCCCCCCEEEEECCCCHHHCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHC
GVSIVTGDTKVVARGAADGLFINTSGVGIIEYPSPLSVASIEPGDAVIVSGTIGDHGATV
CCEEEECCCEEEEECCCCCEEEECCCEEEEECCCCCCEEEECCCCEEEEECCCCCCCHHH
LSKRRELGLISEIRSDSAPLNGLIGSILEASPNVHCMRDPTRGGLGAILAEIAAQSGFRI
HHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEE
TIEETKLPVREEVRGICEILGFDPLFLANEGKVAVFCSPADACLILDKMKNHEYGRDAAL
EEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEEEEHHHCCCCCCCEEE
IGFVGKRNGEGLILNTSVGGSRVVDLPLGELVPRIC
EEEEECCCCCEEEEEECCCCCEEEECCHHHHHHCCC
>Mature Secondary Structure
MDHDRILLEHGGGGLLSHELVTGIFLPLFRNACLERLEDSAVITLGDRKLCFTTDSYVID
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCEEEC
PLFFPGGDIGSLAVHGTINDLSVCGGKPLMMSAGFILEEGFPMEDLRRISRSMAEAAQKA
EEECCCCCCCCEEEEECCCCHHHCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHC
GVSIVTGDTKVVARGAADGLFINTSGVGIIEYPSPLSVASIEPGDAVIVSGTIGDHGATV
CCEEEECCCEEEEECCCCCEEEECCCEEEEECCCCCCEEEECCCCEEEEECCCCCCCHHH
LSKRRELGLISEIRSDSAPLNGLIGSILEASPNVHCMRDPTRGGLGAILAEIAAQSGFRI
HHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEE
TIEETKLPVREEVRGICEILGFDPLFLANEGKVAVFCSPADACLILDKMKNHEYGRDAAL
EEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEEEEHHHCCCCCCCEEE
IGFVGKRNGEGLILNTSVGGSRVVDLPLGELVPRIC
EEEEECCCCCEEEEEECCCCCEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1526470 [H]