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The map label for this gene is pyrB [H]

Identifier: 85860056

GI number: 85860056

Start: 2267425

End: 2268387

Strand: Direct

Name: pyrB [H]

Synonym: SYN_01532

Alternate gene names: 85860056

Gene position: 2267425-2268387 (Clockwise)

Preceding gene: 85860055

Following gene: 85860057

Centisome position: 71.32

GC content: 55.56

Gene sequence:

>963_bases
ATGCATCTGCCGCCAGGTCTCTGTTCCAGGGGTTTTGCAATGAAATGGGAACGTAAGGACATTCTGGGCATGAAAGATCT
TTCCGTAGAGGAAATTCACATGATCCTCGACACGGCGGAGTCGTTTCTCGAAGTATCCGCGCGGGATATCAAAAAAGTGC
CAACGCTCCGGGGAAAGACGATCATCAATTTCTTTGTTGAAGCCAGTACGCGGACCCGAACCTCCTTCGAAATTGCCGGC
AAGCGGCTGAGCGCCGACACCATCAATATCTCCGCATCAGCAAGCAGCCTGGTCAAGGGGGAAACCCTGGCGGACACGGC
GAGAAATCTGGAAGCCATGAATCCCGACGTCATCGTGATCCGTCACAGTTGCGCCGGAGCGCCTCATATGCTGGCCGGAC
TTGTCCGGCAGTCCATCATCAACGCCGGCGACGGCGCTCATGAGCATCCGAGCCAGGCTTTGCTGGACATGATGACAATC
CGAAACCGCAAGGGGGGGCTTTCCGGATTGAACGTGACCATCGTGGGCGATATCGCCCACAGCCGCGTCGCCCGTTCCAA
CATTTACGGGTTGAACAAGATGGGAGTCTGCGTCACCGTGGCCGGCCCCGCCACGATGATTCCCCGGGATATCCAGCAGC
TTGGCGTCAAGGTCTGCTTCAGTCTGGAAGAGGCCATTCTTGATGCGGATGTCATCATGATGCTCAGGATTCAGACGGAG
CGCGAACAGCAGAACATCTTCCCTTCCCTTCGGGAATATTCCACTTTTTATTGCCTGAACAGGGAGAATATCAAAAAAGC
GAAAAAGGATGTCCTGGTGATGCATCCCGGTCCGGTCAACCGCGGCGTGGAAATTTCTCCGGAGATCGCCGACGGCGTTC
ATTCCGTCATTCTGGAACAAGTCACCAACGGGGTCGCGGTCCGGATGGCCCTTCTTTATCTGCTGACAGGAGCCTCTTCA
TGA

Upstream 100 bases:

>100_bases
CCTCTCTTCTCTGGGAGGACATCAGTGTCAACCTGATTGAAAAAAACGGCTGCGATGAAGTGGTCATTGAGGACAGCGGC
CATTAGAATGCATCACTTGC

Downstream 100 bases:

>100_bases
AAATACTGCTGAAGGGGGGACGCGTAATCGACCCCGCGCAAAATCTCGACGGGCAGATGGATCTCCTGCTGGAAAACGGG
AAGATTGCCGCCATAGCCGA

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MHLPPGLCSRGFAMKWERKDILGMKDLSVEEIHMILDTAESFLEVSARDIKKVPTLRGKTIINFFVEASTRTRTSFEIAG
KRLSADTINISASASSLVKGETLADTARNLEAMNPDVIVIRHSCAGAPHMLAGLVRQSIINAGDGAHEHPSQALLDMMTI
RNRKGGLSGLNVTIVGDIAHSRVARSNIYGLNKMGVCVTVAGPATMIPRDIQQLGVKVCFSLEEAILDADVIMMLRIQTE
REQQNIFPSLREYSTFYCLNRENIKKAKKDVLVMHPGPVNRGVEISPEIADGVHSVILEQVTNGVAVRMALLYLLTGASS

Sequences:

>Translated_320_residues
MHLPPGLCSRGFAMKWERKDILGMKDLSVEEIHMILDTAESFLEVSARDIKKVPTLRGKTIINFFVEASTRTRTSFEIAG
KRLSADTINISASASSLVKGETLADTARNLEAMNPDVIVIRHSCAGAPHMLAGLVRQSIINAGDGAHEHPSQALLDMMTI
RNRKGGLSGLNVTIVGDIAHSRVARSNIYGLNKMGVCVTVAGPATMIPRDIQQLGVKVCFSLEEAILDADVIMMLRIQTE
REQQNIFPSLREYSTFYCLNRENIKKAKKDVLVMHPGPVNRGVEISPEIADGVHSVILEQVTNGVAVRMALLYLLTGASS
>Mature_320_residues
MHLPPGLCSRGFAMKWERKDILGMKDLSVEEIHMILDTAESFLEVSARDIKKVPTLRGKTIINFFVEASTRTRTSFEIAG
KRLSADTINISASASSLVKGETLADTARNLEAMNPDVIVIRHSCAGAPHMLAGLVRQSIINAGDGAHEHPSQALLDMMTI
RNRKGGLSGLNVTIVGDIAHSRVARSNIYGLNKMGVCVTVAGPATMIPRDIQQLGVKVCFSLEEAILDADVIMMLRIQTE
REQQNIFPSLREYSTFYCLNRENIKKAKKDVLVMHPGPVNRGVEISPEIADGVHSVILEQVTNGVAVRMALLYLLTGASS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=35.5987055016181, Blast_Score=166, Evalue=2e-41,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=32.8025477707006, Blast_Score=143, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI193204318, Length=346, Percent_Identity=32.9479768786127, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=33.2258064516129, Blast_Score=145, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24642586, Length=310, Percent_Identity=34.5161290322581, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34930; Mature: 34930

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLPPGLCSRGFAMKWERKDILGMKDLSVEEIHMILDTAESFLEVSARDIKKVPTLRGKT
CCCCCCCCCCCCCEEECCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IINFFVEASTRTRTSFEIAGKRLSADTINISASASSLVKGETLADTARNLEAMNPDVIVI
EEEEEEECCCCCCCHHHHHCCEECCCEEEEECCHHHHHCCCHHHHHHHHHHHCCCCEEEE
RHSCAGAPHMLAGLVRQSIINAGDGAHEHPSQALLDMMTIRNRKGGLSGLNVTIVGDIAH
ECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECHHH
SRVARSNIYGLNKMGVCVTVAGPATMIPRDIQQLGVKVCFSLEEAILDADVIMMLRIQTE
HHHHHHHCCCCCCCCEEEEECCCHHHCHHHHHHHHHHHHHHHHHHHHCHHEEEEEEEHHH
REQQNIFPSLREYSTFYCLNRENIKKAKKDVLVMHPGPVNRGVEISPEIADGVHSVILEQ
HHHHHHCHHHHHCCEEEEECHHHHHHHHCCEEEECCCCCCCCCEECCHHHHHHHHHHHHH
VTNGVAVRMALLYLLTGASS
HHCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHLPPGLCSRGFAMKWERKDILGMKDLSVEEIHMILDTAESFLEVSARDIKKVPTLRGKT
CCCCCCCCCCCCCEEECCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IINFFVEASTRTRTSFEIAGKRLSADTINISASASSLVKGETLADTARNLEAMNPDVIVI
EEEEEEECCCCCCCHHHHHCCEECCCEEEEECCHHHHHCCCHHHHHHHHHHHCCCCEEEE
RHSCAGAPHMLAGLVRQSIINAGDGAHEHPSQALLDMMTIRNRKGGLSGLNVTIVGDIAH
ECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECHHH
SRVARSNIYGLNKMGVCVTVAGPATMIPRDIQQLGVKVCFSLEEAILDADVIMMLRIQTE
HHHHHHHCCCCCCCCEEEEECCCHHHCHHHHHHHHHHHHHHHHHHHHCHHEEEEEEEHHH
REQQNIFPSLREYSTFYCLNRENIKKAKKDVLVMHPGPVNRGVEISPEIADGVHSVILEQ
HHHHHHCHHHHHCCEEEEECHHHHHHHHCCEEEECCCCCCCCCEECCHHHHHHHHHHHHH
VTNGVAVRMALLYLLTGASS
HHCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA