Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 85859641

GI number: 85859641

Start: 1868414

End: 1870348

Strand: Direct

Name: metG [H]

Synonym: SYN_00558

Alternate gene names: 85859641

Gene position: 1868414-1870348 (Clockwise)

Preceding gene: 85859640

Following gene: 85859645

Centisome position: 58.77

GC content: 52.2

Gene sequence:

>1935_bases
TTGAGTGTCTTCTGTGTAGAAAGGATTTTTATGGAAAAAACGTTTTACATCACAACGCCAATCTATTATGTCAATGCTTC
TCCGCATATCGGTCATGCCTATACCACCATCGTTGCCGATGTTCTGGCCCGTTATCACCGTCTTTCGGGACACCGGACGT
TTTTCCTGACAGGCACGGATGAACACGGGGACAAAATCGCCGAGGCAGCGGAAAAAAACGGCATTTCTCCCAAAGCCTAC
GCGGACCGGATCAGCAGTCAGTTCAGAAATCTCTGGCCTGAACTTTCAGTCAGCAACGATTATTTTATTCGGACAACAGA
CGCCAATCACATTGCCACGGTTCAGGCGATTCTCTCCAAGGTTTACGAAGCCGGCGATATTTATTTCGGTCATTACGAAG
GCTTTTACTGTGTCGGTTGTGAACGCTTCTATACCGAAAAGGAGCTCATTGACGGAAAATGTCCGGATCATCAGATGGAA
CCGGAATTTCGCAAGGAAAGCAATTATTTCTTCAAAATGAGCAAGTACCAGGACTGGCTGATTTCGCATATCAAAGCGAA
TCCCGATTTCATCCGGCCTGAACGTTACCGCAATGAAGTTCTCGCCTTCCTCAGGGAGCCTCTGGAAGATCTCTGTATTT
CCCGGCCAAAATCCCGGCTCGCCTGGGGGATTTCCCTTCCCTTCGATGCAAATTATGTCACCTACGTCTGGTTTGACGCC
CTGATCAACTATATCACCGGGCTCGGCTACCCTGACGGAGAGAATTTCCACACGTTCTGGCCGGTCGCACAACACCTGAT
CGCCAAGGACATCCTTAAGCCGCACGGCATTTACTGGCCCACGATGCTGAAGGCCGCCGGAATCGAGCCTTACAGACATC
TCAATGTCCACGGCTACTGGAATGTCGACCAGAGCAAGATGTCCAAGAGCCTCGGAAATGTCATCCGGCCTCTTGATCTG
AAGGACAAGTACGGACTGGATGCCTTCCGTTATTACCTCCTGCGGGAGATGGTCTTCGGCCTGGATTCCAGCTTCAGTGA
AGAAGGATTTGTCCAGAGGGTCAATGCGGATCTGGCCAACGATCTGGGAAATCTTGTCAGCCGGGTAACGGCCATGGCTG
TTAAATACTGCAAAGGAAGCGTTCCGGCGGCATCATCAGAGGCAGACACGATTCTTTCCAGTACGGCAGTCAGGGTGATT
CAGGAAGTGGAGGCGTCGTTCCGGGAGCTGACTTTCCACAAGGCCCTGATGTCCATCTGGGATCTGATCAATACGGCAAA
CCGCTACATTGTGGAAAAGGAACCATGGAATCTGGCCAAGGATCCGGCAAATCAGGGCACACTCGATGACGTGATCTATT
CACTGCTGGAGACATTGCGGATTGTTTCGGTTCTGATCGCCCCGTTCATGCCGGGATCTTCCGTGAAGATCATGACCTCA
CTGGGCATCGCCAATCCGGAGCTTCAAAATTTCGACATGATTCGCACCTGGGGAGGTCTTGCCGCCGGTCAAACCCTGGA
ACGCGGTGAAGCACTTTTCCCCCGGATTGATTACAAACCGGACGAGGGTGCCGCCGAGATCAAAGCCACTCCCCCTCCCC
CGCCGATCAAGCCGGAAGTTTCCTATGAAGACTTTGAAAAGATTGATCTGCGGGTCGCCCGGATTCTCGAAGCCGAAGCC
GTTCCGAAATCAGCCAAACTTCTGAAGCTCAAGATCGACATCGGAGAAGAAAGGACGATTATCGCCGGTGTCGCCAAAGA
CTATACGCCGGACCAGCTGGTGGGAAAATCCATTGTCATCGTGGCCAATCTCAAACCAGCCAAGCTGATGGGAATCGAAT
CCCGGGGCATGCTTCTCGCCACGGATGCAACAGCAGGCGGAATTACCGTGCTCGCCTTCGACAGGGAGCCGATAACGGGC
TCGAAAGTCCGGTAA

Upstream 100 bases:

>100_bases
AGAAAAAAGAAAAAAGCGTATCGGGCCGGAAAGATAAAGACGGCCCCCCCAAACCGGGAGATGGGGAAAAGACGCCTTTG
GAACCGGCTGCTGTTGAACA

Downstream 100 bases:

>100_bases
TTTCAATTAGACATCAATGTTAAATCGGCGTTGGCTCCTTTATCGTCTGCTCCACATACTTGCCATGTGAGCCCGAGTCA
CCACCTGAACATAGTCGGCC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MSVFCVERIFMEKTFYITTPIYYVNASPHIGHAYTTIVADVLARYHRLSGHRTFFLTGTDEHGDKIAEAAEKNGISPKAY
ADRISSQFRNLWPELSVSNDYFIRTTDANHIATVQAILSKVYEAGDIYFGHYEGFYCVGCERFYTEKELIDGKCPDHQME
PEFRKESNYFFKMSKYQDWLISHIKANPDFIRPERYRNEVLAFLREPLEDLCISRPKSRLAWGISLPFDANYVTYVWFDA
LINYITGLGYPDGENFHTFWPVAQHLIAKDILKPHGIYWPTMLKAAGIEPYRHLNVHGYWNVDQSKMSKSLGNVIRPLDL
KDKYGLDAFRYYLLREMVFGLDSSFSEEGFVQRVNADLANDLGNLVSRVTAMAVKYCKGSVPAASSEADTILSSTAVRVI
QEVEASFRELTFHKALMSIWDLINTANRYIVEKEPWNLAKDPANQGTLDDVIYSLLETLRIVSVLIAPFMPGSSVKIMTS
LGIANPELQNFDMIRTWGGLAAGQTLERGEALFPRIDYKPDEGAAEIKATPPPPPIKPEVSYEDFEKIDLRVARILEAEA
VPKSAKLLKLKIDIGEERTIIAGVAKDYTPDQLVGKSIVIVANLKPAKLMGIESRGMLLATDATAGGITVLAFDREPITG
SKVR

Sequences:

>Translated_644_residues
MSVFCVERIFMEKTFYITTPIYYVNASPHIGHAYTTIVADVLARYHRLSGHRTFFLTGTDEHGDKIAEAAEKNGISPKAY
ADRISSQFRNLWPELSVSNDYFIRTTDANHIATVQAILSKVYEAGDIYFGHYEGFYCVGCERFYTEKELIDGKCPDHQME
PEFRKESNYFFKMSKYQDWLISHIKANPDFIRPERYRNEVLAFLREPLEDLCISRPKSRLAWGISLPFDANYVTYVWFDA
LINYITGLGYPDGENFHTFWPVAQHLIAKDILKPHGIYWPTMLKAAGIEPYRHLNVHGYWNVDQSKMSKSLGNVIRPLDL
KDKYGLDAFRYYLLREMVFGLDSSFSEEGFVQRVNADLANDLGNLVSRVTAMAVKYCKGSVPAASSEADTILSSTAVRVI
QEVEASFRELTFHKALMSIWDLINTANRYIVEKEPWNLAKDPANQGTLDDVIYSLLETLRIVSVLIAPFMPGSSVKIMTS
LGIANPELQNFDMIRTWGGLAAGQTLERGEALFPRIDYKPDEGAAEIKATPPPPPIKPEVSYEDFEKIDLRVARILEAEA
VPKSAKLLKLKIDIGEERTIIAGVAKDYTPDQLVGKSIVIVANLKPAKLMGIESRGMLLATDATAGGITVLAFDREPITG
SKVR
>Mature_643_residues
SVFCVERIFMEKTFYITTPIYYVNASPHIGHAYTTIVADVLARYHRLSGHRTFFLTGTDEHGDKIAEAAEKNGISPKAYA
DRISSQFRNLWPELSVSNDYFIRTTDANHIATVQAILSKVYEAGDIYFGHYEGFYCVGCERFYTEKELIDGKCPDHQMEP
EFRKESNYFFKMSKYQDWLISHIKANPDFIRPERYRNEVLAFLREPLEDLCISRPKSRLAWGISLPFDANYVTYVWFDAL
INYITGLGYPDGENFHTFWPVAQHLIAKDILKPHGIYWPTMLKAAGIEPYRHLNVHGYWNVDQSKMSKSLGNVIRPLDLK
DKYGLDAFRYYLLREMVFGLDSSFSEEGFVQRVNADLANDLGNLVSRVTAMAVKYCKGSVPAASSEADTILSSTAVRVIQ
EVEASFRELTFHKALMSIWDLINTANRYIVEKEPWNLAKDPANQGTLDDVIYSLLETLRIVSVLIAPFMPGSSVKIMTSL
GIANPELQNFDMIRTWGGLAAGQTLERGEALFPRIDYKPDEGAAEIKATPPPPPIKPEVSYEDFEKIDLRVARILEAEAV
PKSAKLLKLKIDIGEERTIIAGVAKDYTPDQLVGKSIVIVANLKPAKLMGIESRGMLLATDATAGGITVLAFDREPITGS
KVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=537, Percent_Identity=36.6852886405959, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI14043022, Length=643, Percent_Identity=26.1275272161742, Blast_Score=162, Evalue=8e-40,
Organism=Escherichia coli, GI1788432, Length=689, Percent_Identity=28.3018867924528, Blast_Score=249, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI212645096, Length=523, Percent_Identity=34.4168260038241, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI71992781, Length=408, Percent_Identity=31.3725490196078, Blast_Score=188, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17540794, Length=553, Percent_Identity=25.6781193490054, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321610, Length=530, Percent_Identity=33.0188679245283, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6321704, Length=558, Percent_Identity=25.4480286738351, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI21358483, Length=509, Percent_Identity=36.3457760314342, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24655311, Length=554, Percent_Identity=25.4512635379061, Blast_Score=163, Evalue=4e-40,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 72676; Mature: 72545

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFCVERIFMEKTFYITTPIYYVNASPHIGHAYTTIVADVLARYHRLSGHRTFFLTGTD
CCCHHHHHHHHCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
EHGDKIAEAAEKNGISPKAYADRISSQFRNLWPELSVSNDYFIRTTDANHIATVQAILSK
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VYEAGDIYFGHYEGFYCVGCERFYTEKELIDGKCPDHQMEPEFRKESNYFFKMSKYQDWL
HHHCCCEEEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEHHHHHHH
ISHIKANPDFIRPERYRNEVLAFLREPLEDLCISRPKSRLAWGISLPFDANYVTYVWFDA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCCHHHHHHHH
LINYITGLGYPDGENFHTFWPVAQHLIAKDILKPHGIYWPTMLKAAGIEPYRHLNVHGYW
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEE
NVDQSKMSKSLGNVIRPLDLKDKYGLDAFRYYLLREMVFGLDSSFSEEGFVQRVNADLAN
CCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
DLGNLVSRVTAMAVKYCKGSVPAASSEADTILSSTAVRVIQEVEASFRELTFHKALMSIW
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLINTANRYIVEKEPWNLAKDPANQGTLDDVIYSLLETLRIVSVLIAPFMPGSSVKIMTS
HHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
LGIANPELQNFDMIRTWGGLAAGQTLERGEALFPRIDYKPDEGAAEIKATPPPPPIKPEV
CCCCCCCCCCCHHHHHHCCHHCCCHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
SYEDFEKIDLRVARILEAEAVPKSAKLLKLKIDIGEERTIIAGVAKDYTPDQLVGKSIVI
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCHHHCCCEEEE
VANLKPAKLMGIESRGMLLATDATAGGITVLAFDREPITGSKVR
EECCCCHHEEEECCCCEEEEECCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SVFCVERIFMEKTFYITTPIYYVNASPHIGHAYTTIVADVLARYHRLSGHRTFFLTGTD
CCHHHHHHHHCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
EHGDKIAEAAEKNGISPKAYADRISSQFRNLWPELSVSNDYFIRTTDANHIATVQAILSK
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VYEAGDIYFGHYEGFYCVGCERFYTEKELIDGKCPDHQMEPEFRKESNYFFKMSKYQDWL
HHHCCCEEEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEHHHHHHH
ISHIKANPDFIRPERYRNEVLAFLREPLEDLCISRPKSRLAWGISLPFDANYVTYVWFDA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCCHHHHHHHH
LINYITGLGYPDGENFHTFWPVAQHLIAKDILKPHGIYWPTMLKAAGIEPYRHLNVHGYW
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEE
NVDQSKMSKSLGNVIRPLDLKDKYGLDAFRYYLLREMVFGLDSSFSEEGFVQRVNADLAN
CCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
DLGNLVSRVTAMAVKYCKGSVPAASSEADTILSSTAVRVIQEVEASFRELTFHKALMSIW
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLINTANRYIVEKEPWNLAKDPANQGTLDDVIYSLLETLRIVSVLIAPFMPGSSVKIMTS
HHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
LGIANPELQNFDMIRTWGGLAAGQTLERGEALFPRIDYKPDEGAAEIKATPPPPPIKPEV
CCCCCCCCCCCHHHHHHCCHHCCCHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
SYEDFEKIDLRVARILEAEAVPKSAKLLKLKIDIGEERTIIAGVAKDYTPDQLVGKSIVI
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCHHHCCCEEEE
VANLKPAKLMGIESRGMLLATDATAGGITVLAFDREPITGSKVR
EECCCCHHEEEECCCCEEEEECCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1993699; 10673435 [H]