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The map label for this gene is ispH [H]

Identifier: 85859633

GI number: 85859633

Start: 1862575

End: 1864308

Strand: Direct

Name: ispH [H]

Synonym: SYN_02454

Alternate gene names: 85859633

Gene position: 1862575-1864308 (Clockwise)

Preceding gene: 85859632

Following gene: 85859634

Centisome position: 58.58

GC content: 52.6

Gene sequence:

>1734_bases
ATGAGTGTCTTTCTCGCAAAGACGGCAGGGTTCTGCATGGGCGTCAAACGTGCCGTGGATACGGTTCTGGATATCGCCCG
CCACAAGGGATCGGAAAAAATCTATACCTACGGTCCTCTGATTCACAATCCTCAAACTGTCGAATTCCTGAGGGAAAGAG
GAATTATTCCCATTAAGGATATCGAGGCCCTTTCTGCATCGGATCAAAATGCGGCCATCGTCATCCGAGCTCATGGCATC
TCGCCGAGCGAACGAAAGAAGATCCGGAATAAAGGGCTGCGGATCATCGACGCAACCTGTCCGAAAGTCACCCATGTCCA
GGCGATTATCCGGCAGCATGCCGCAAAAGATTACAACATCCTGATCATCGGTGACGGCGACCACCCTGAAGTCAACGGCC
TTCTCGGACATGCCGGCGGTCATGGCCGGGTCATCGGCAGCCTCCAGAACGTTGAAGAACTTCCGATTATGGAAAAGGTC
TGCGTCGTTGCCCAGACCACTCAAAACGTTGAAGAATATGCTGCAATCGTCCGCCGGATCCGCGATCGATTTCCGGAAAC
CGTCGTGTTTAATACCATCTGTGATTCCACGGAACAGCGGCAGGCTGAAGTTCGCGAACTTGCCGCCAGAATGGAGGCCA
TGGTGATCGTGGGCGGCAGGAACAGCGCCAATACACGGCGTCTGGCCGCCCTGTCCTCTCAGCAGGGCACTCCGACCTTT
CATATCGAGACAGCCGATGAACTTAACGACGCGAAACTGGCTCCCTATGACCGGATCGGACTTTCTGCCGGAGCCTCCAC
GCCCAACTGGATCATTGACCGGGTTGTCGACCGCATTGCGGAACAGCAGAGGAAAAAGAAAAATAAGGGGAAGGGACTGC
TGAAGCTCTGGACCCTTTCAGTGAGGACCGATCTCTATTCCGCCCTTGGGGCGGCTTGTCTGTCCCTGACCGCCATGCTT
CTGGAACGGGTCCCGGTGAATATCCTTTTCCTTCTGATCACCGCCCTATATGTCCATGCGATGCATACCTTCAACAGGTT
TTCGAACCGAAAGACGTGCAGCATCGGTTCCTTCCGCGAAGAATCCTACCTGAAGCACGAGAATTTTTATCTTTCCGGAG
CGCTTGCCTCCATGCTGCTCTGTCTTGGTCTTGCCCTGAGTCATGGGATGCGGACCTTTCTTCTGCTTTTTTTCATATCG
GGGTTGGGCATTCTCTACAATCTGCCCATCTTTCCCGCAAAATGGAGATATCGCAGCTTAAAGGATCTGCCCGGATCAAA
AAACATCTCCATGGCCATGGCGTGGGCCACTGTTGTTGCCATCCTGCCCGGCATGGAGCGGGGGTTGTTTGTTTCGCCGG
GAATTGCCATCGCCTTTCTTTTCACCATGGGCATCGTGTTTATCCGTTCCTCTCTTTCGGATATTCTGGATATTCAGCGC
GACAGGCTGATTGGCAAGGAAACGATTCCCGTGCTCATCGGCAGAGAACCAACACAGGTTCTCCTGACAATACTGTCCAT
TTTTATTTTTTTTCTACTGATAGCGGGTGCGTCCGCGAAATGGACTCCATCGCTTTCACTGGTCCTTCCGGCCAACTTAT
TTTATGTATGGATTTGTTTTAAACTTTGTGATAGAAGGGCGTCGTTTTCCGGGGTTGTTCTGGAGGGTCTTCTGGAAACC
AGCTATATTATCGCCGGGTTGAGTTCACTGCTGTGGTGCGCCCTATCCCGTTAA

Upstream 100 bases:

>100_bases
CTGCCCGGCTGCCGGAAGAAAAACTTTCATTTCCCGGTCGGCTGGAGTCTTCTCCTGCAAGTCAGGAAACCGCTCAGGAC
AAGGAGCTGTTGTCAAAACA

Downstream 100 bases:

>100_bases
ACAAAAGAAGTGAGCGACCCTTGTGTTTGCAGGAGTGTCCCGTGAAGAGAAGATACTGGAACGCTTTCATTGCCCTTTTC
CTGATTCTGATGATTTCCGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MSVFLAKTAGFCMGVKRAVDTVLDIARHKGSEKIYTYGPLIHNPQTVEFLRERGIIPIKDIEALSASDQNAAIVIRAHGI
SPSERKKIRNKGLRIIDATCPKVTHVQAIIRQHAAKDYNILIIGDGDHPEVNGLLGHAGGHGRVIGSLQNVEELPIMEKV
CVVAQTTQNVEEYAAIVRRIRDRFPETVVFNTICDSTEQRQAEVRELAARMEAMVIVGGRNSANTRRLAALSSQQGTPTF
HIETADELNDAKLAPYDRIGLSAGASTPNWIIDRVVDRIAEQQRKKKNKGKGLLKLWTLSVRTDLYSALGAACLSLTAML
LERVPVNILFLLITALYVHAMHTFNRFSNRKTCSIGSFREESYLKHENFYLSGALASMLLCLGLALSHGMRTFLLLFFIS
GLGILYNLPIFPAKWRYRSLKDLPGSKNISMAMAWATVVAILPGMERGLFVSPGIAIAFLFTMGIVFIRSSLSDILDIQR
DRLIGKETIPVLIGREPTQVLLTILSIFIFFLLIAGASAKWTPSLSLVLPANLFYVWICFKLCDRRASFSGVVLEGLLET
SYIIAGLSSLLWCALSR

Sequences:

>Translated_577_residues
MSVFLAKTAGFCMGVKRAVDTVLDIARHKGSEKIYTYGPLIHNPQTVEFLRERGIIPIKDIEALSASDQNAAIVIRAHGI
SPSERKKIRNKGLRIIDATCPKVTHVQAIIRQHAAKDYNILIIGDGDHPEVNGLLGHAGGHGRVIGSLQNVEELPIMEKV
CVVAQTTQNVEEYAAIVRRIRDRFPETVVFNTICDSTEQRQAEVRELAARMEAMVIVGGRNSANTRRLAALSSQQGTPTF
HIETADELNDAKLAPYDRIGLSAGASTPNWIIDRVVDRIAEQQRKKKNKGKGLLKLWTLSVRTDLYSALGAACLSLTAML
LERVPVNILFLLITALYVHAMHTFNRFSNRKTCSIGSFREESYLKHENFYLSGALASMLLCLGLALSHGMRTFLLLFFIS
GLGILYNLPIFPAKWRYRSLKDLPGSKNISMAMAWATVVAILPGMERGLFVSPGIAIAFLFTMGIVFIRSSLSDILDIQR
DRLIGKETIPVLIGREPTQVLLTILSIFIFFLLIAGASAKWTPSLSLVLPANLFYVWICFKLCDRRASFSGVVLEGLLET
SYIIAGLSSLLWCALSR
>Mature_576_residues
SVFLAKTAGFCMGVKRAVDTVLDIARHKGSEKIYTYGPLIHNPQTVEFLRERGIIPIKDIEALSASDQNAAIVIRAHGIS
PSERKKIRNKGLRIIDATCPKVTHVQAIIRQHAAKDYNILIIGDGDHPEVNGLLGHAGGHGRVIGSLQNVEELPIMEKVC
VVAQTTQNVEEYAAIVRRIRDRFPETVVFNTICDSTEQRQAEVRELAARMEAMVIVGGRNSANTRRLAALSSQQGTPTFH
IETADELNDAKLAPYDRIGLSAGASTPNWIIDRVVDRIAEQQRKKKNKGKGLLKLWTLSVRTDLYSALGAACLSLTAMLL
ERVPVNILFLLITALYVHAMHTFNRFSNRKTCSIGSFREESYLKHENFYLSGALASMLLCLGLALSHGMRTFLLLFFISG
LGILYNLPIFPAKWRYRSLKDLPGSKNISMAMAWATVVAILPGMERGLFVSPGIAIAFLFTMGIVFIRSSLSDILDIQRD
RLIGKETIPVLIGREPTQVLLTILSIFIFFLLIAGASAKWTPSLSLVLPANLFYVWICFKLCDRRASFSGVVLEGLLETS
YIIAGLSSLLWCALSR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=288, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 63851; Mature: 63720

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFLAKTAGFCMGVKRAVDTVLDIARHKGSEKIYTYGPLIHNPQTVEFLRERGIIPIKD
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCHH
IEALSASDQNAAIVIRAHGISPSERKKIRNKGLRIIDATCPKVTHVQAIIRQHAAKDYNI
HHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCEE
LIIGDGDHPEVNGLLGHAGGHGRVIGSLQNVEELPIMEKVCVVAQTTQNVEEYAAIVRRI
EEEECCCCCCCCCEEECCCCCCCEECCHHCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHH
RDRFPETVVFNTICDSTEQRQAEVRELAARMEAMVIVGGRNSANTRRLAALSSQQGTPTF
HHHCCCCEEHHHHHCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHCCCCCCCEE
HIETADELNDAKLAPYDRIGLSAGASTPNWIIDRVVDRIAEQQRKKKNKGKGLLKLWTLS
EEECCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHH
VRTDLYSALGAACLSLTAMLLERVPVNILFLLITALYVHAMHTFNRFSNRKTCSIGSFRE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
ESYLKHENFYLSGALASMLLCLGLALSHGMRTFLLLFFISGLGILYNLPIFPAKWRYRSL
HHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KDLPGSKNISMAMAWATVVAILPGMERGLFVSPGIAIAFLFTMGIVFIRSSLSDILDIQR
HCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRLIGKETIPVLIGREPTQVLLTILSIFIFFLLIAGASAKWTPSLSLVLPANLFYVWICF
HHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHH
KLCDRRASFSGVVLEGLLETSYIIAGLSSLLWCALSR
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVFLAKTAGFCMGVKRAVDTVLDIARHKGSEKIYTYGPLIHNPQTVEFLRERGIIPIKD
CEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCHH
IEALSASDQNAAIVIRAHGISPSERKKIRNKGLRIIDATCPKVTHVQAIIRQHAAKDYNI
HHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCEE
LIIGDGDHPEVNGLLGHAGGHGRVIGSLQNVEELPIMEKVCVVAQTTQNVEEYAAIVRRI
EEEECCCCCCCCCEEECCCCCCCEECCHHCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHH
RDRFPETVVFNTICDSTEQRQAEVRELAARMEAMVIVGGRNSANTRRLAALSSQQGTPTF
HHHCCCCEEHHHHHCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHCCCCCCCEE
HIETADELNDAKLAPYDRIGLSAGASTPNWIIDRVVDRIAEQQRKKKNKGKGLLKLWTLS
EEECCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHH
VRTDLYSALGAACLSLTAMLLERVPVNILFLLITALYVHAMHTFNRFSNRKTCSIGSFRE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
ESYLKHENFYLSGALASMLLCLGLALSHGMRTFLLLFFISGLGILYNLPIFPAKWRYRSL
HHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KDLPGSKNISMAMAWATVVAILPGMERGLFVSPGIAIAFLFTMGIVFIRSSLSDILDIQR
HCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRLIGKETIPVLIGREPTQVLLTILSIFIFFLLIAGASAKWTPSLSLVLPANLFYVWICF
HHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHH
KLCDRRASFSGVVLEGLLETSYIIAGLSSLLWCALSR
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA