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The map label for this gene is rfbB [H]

Identifier: 85859148

GI number: 85859148

Start: 1324108

End: 1325199

Strand: Direct

Name: rfbB [H]

Synonym: SYN_02866

Alternate gene names: 85859148

Gene position: 1324108-1325199 (Clockwise)

Preceding gene: 85859147

Following gene: 85859149

Centisome position: 41.65

GC content: 56.14

Gene sequence:

>1092_bases
ATGAAAATAGAGAATCTGATGGTGACAGGGGGCTGCGGGTTTATCGGCAGCAACTTTATTCGATATCTGCTGGAAGAGGC
GAATTTTGCGGGGCGGATTGTCAATGTGGACAAGTTGACGTACGCGGGCAATCCGGAAAATCTGGCGGGGCTGGCAGAAA
AATTCGGGGAGCGATACTGCTTTGAAAAGGCGGATATCTGCGATGCCGACCGGATGAAGGCAATTTTTGCGGAATACGAA
ATCGACGCCGTCTGCCATTTTGCCGCCGAGTCTCATGTGGACCGGTCGATCCGCAGGCCGGAGAGCTTCATCCAGACCAA
CATCGTGGGGACATTCAATCTGCTCGAACTGGCCAGGGCGCGGGGGGATCGCTTCCAGATTTTCCATCACGTCAGCACGG
ACGAGGTTTATGGCAGCCTGGGGCCGGAAGGCTACTTCACGGAGGAGACTCCGTATCGGCCTAACAGTCCCTATTCCGCT
TCCAAGGCGTCGTCGGATCACTTGGTACGGGCCTATCACGTGACTTATGGCCTTCCCATTACCCTTTCCAACTGCTCCAA
CAATTACGGACCCTATCAGTTTCCCGAGAAGCTCATCCCCCTGATCGTCATCAACGCCCTGGAAGGCAAGACCCTCCCGG
TTTACGGCGACGGGAAGAACGTCCGGGACTGGCTGTATGTCCGCGATCATTGCGAGGCGATCTGGACGATCATGAATTCC
GGCCGCCGGGGGGAAACCTACAACGTGGGCGGCCAGTCTGAGATGGAGAACATCGCCATTGTCCGGCTGATCTGTGATAT
CCTCGATGAAGTGCGTCCGGCGGGTGATGGGAAATCCCGGCAGGAGCTGATCACCTTCGTGGCGGACCGTCCCGGGCATG
ATCACCGCTACGCCATCGATTTTACGAAACTGAATGCGGAATTGAAGTGGAGTCCCCGGGAGTCCTTTACCACGGGGTTG
AGACGGACGATCAACTGGTATCTCGACAATCCCGGATGGATCGACCGGATACGCAGCGGGGCATATGCCGCATGGATCGA
TGAACAATACGGGGATTCATCCGTCAGTCAGACAGCAGCCGTGGAAAAATAG

Upstream 100 bases:

>100_bases
GAAGCTCATGAGCCTCGGCCTTTCCTACGACGACGCCGTCAACCGCATCATCCAGGGCTTCCTGAAGTAGAAAGCGAGGG
GTGAGGAGAGATAAATCGCA

Downstream 100 bases:

>100_bases
AGCGGTTGAAACAGGCCGGAAAAAACAAGGAGAAATCGGAGGACAGGAAAAGAATGGATGCCTTTCTCAATGAACCGATG
GCCTTCCGGGGGATTCTCTG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKIENLMVTGGCGFIGSNFIRYLLEEANFAGRIVNVDKLTYAGNPENLAGLAEKFGERYCFEKADICDADRMKAIFAEYE
IDAVCHFAAESHVDRSIRRPESFIQTNIVGTFNLLELARARGDRFQIFHHVSTDEVYGSLGPEGYFTEETPYRPNSPYSA
SKASSDHLVRAYHVTYGLPITLSNCSNNYGPYQFPEKLIPLIVINALEGKTLPVYGDGKNVRDWLYVRDHCEAIWTIMNS
GRRGETYNVGGQSEMENIAIVRLICDILDEVRPAGDGKSRQELITFVADRPGHDHRYAIDFTKLNAELKWSPRESFTTGL
RRTINWYLDNPGWIDRIRSGAYAAWIDEQYGDSSVSQTAAVEK

Sequences:

>Translated_363_residues
MKIENLMVTGGCGFIGSNFIRYLLEEANFAGRIVNVDKLTYAGNPENLAGLAEKFGERYCFEKADICDADRMKAIFAEYE
IDAVCHFAAESHVDRSIRRPESFIQTNIVGTFNLLELARARGDRFQIFHHVSTDEVYGSLGPEGYFTEETPYRPNSPYSA
SKASSDHLVRAYHVTYGLPITLSNCSNNYGPYQFPEKLIPLIVINALEGKTLPVYGDGKNVRDWLYVRDHCEAIWTIMNS
GRRGETYNVGGQSEMENIAIVRLICDILDEVRPAGDGKSRQELITFVADRPGHDHRYAIDFTKLNAELKWSPRESFTTGL
RRTINWYLDNPGWIDRIRSGAYAAWIDEQYGDSSVSQTAAVEK
>Mature_363_residues
MKIENLMVTGGCGFIGSNFIRYLLEEANFAGRIVNVDKLTYAGNPENLAGLAEKFGERYCFEKADICDADRMKAIFAEYE
IDAVCHFAAESHVDRSIRRPESFIQTNIVGTFNLLELARARGDRFQIFHHVSTDEVYGSLGPEGYFTEETPYRPNSPYSA
SKASSDHLVRAYHVTYGLPITLSNCSNNYGPYQFPEKLIPLIVINALEGKTLPVYGDGKNVRDWLYVRDHCEAIWTIMNS
GRRGETYNVGGQSEMENIAIVRLICDILDEVRPAGDGKSRQELITFVADRPGHDHRYAIDFTKLNAELKWSPRESFTTGL
RRTINWYLDNPGWIDRIRSGAYAAWIDEQYGDSSVSQTAAVEK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=36.697247706422, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=24.773413897281, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56237023, Length=251, Percent_Identity=30.6772908366534, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI56118217, Length=251, Percent_Identity=30.6772908366534, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI189083684, Length=251, Percent_Identity=30.6772908366534, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4504031, Length=338, Percent_Identity=24.8520710059172, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=56.6298342541437, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=54.8295454545455, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1786974, Length=352, Percent_Identity=25.5681818181818, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788366, Length=242, Percent_Identity=25.6198347107438, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=326, Percent_Identity=35.8895705521472, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=326, Percent_Identity=34.3558282208589, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17539532, Length=350, Percent_Identity=24, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=360, Percent_Identity=26.3888888888889, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=358, Percent_Identity=25.9776536312849, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI133901786, Length=240, Percent_Identity=25.8333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=186, Percent_Identity=27.9569892473118, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=186, Percent_Identity=27.9569892473118, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=240, Percent_Identity=25.8333333333333, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=245, Percent_Identity=26.1224489795918, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=186, Percent_Identity=27.9569892473118, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=249, Percent_Identity=27.710843373494, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356223, Length=345, Percent_Identity=25.7971014492754, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=28.4530386740331, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24158427, Length=326, Percent_Identity=24.8466257668712, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41031; Mature: 41031

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIENLMVTGGCGFIGSNFIRYLLEEANFAGRIVNVDKLTYAGNPENLAGLAEKFGERYC
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHC
FEKADICDADRMKAIFAEYEIDAVCHFAAESHVDRSIRRPESFIQTNIVGTFNLLELARA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHH
RGDRFQIFHHVSTDEVYGSLGPEGYFTEETPYRPNSPYSASKASSDHLVRAYHVTYGLPI
CCCEEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCEE
TLSNCSNNYGPYQFPEKLIPLIVINALEGKTLPVYGDGKNVRDWLYVRDHCEAIWTIMNS
EEECCCCCCCCCCCHHHHHHEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
GRRGETYNVGGQSEMENIAIVRLICDILDEVRPAGDGKSRQELITFVADRPGHDHRYAID
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEE
FTKLNAELKWSPRESFTTGLRRTINWYLDNPGWIDRIRSGAYAAWIDEQYGDSSVSQTAA
EEEECCEEEECCHHHHHHHHHHEEEEEECCCHHHHHHHCCCEEEEECHHCCCCHHHHHHC
VEK
CCC
>Mature Secondary Structure
MKIENLMVTGGCGFIGSNFIRYLLEEANFAGRIVNVDKLTYAGNPENLAGLAEKFGERYC
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHC
FEKADICDADRMKAIFAEYEIDAVCHFAAESHVDRSIRRPESFIQTNIVGTFNLLELARA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHH
RGDRFQIFHHVSTDEVYGSLGPEGYFTEETPYRPNSPYSASKASSDHLVRAYHVTYGLPI
CCCEEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCEE
TLSNCSNNYGPYQFPEKLIPLIVINALEGKTLPVYGDGKNVRDWLYVRDHCEAIWTIMNS
EEECCCCCCCCCCCHHHHHHEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
GRRGETYNVGGQSEMENIAIVRLICDILDEVRPAGDGKSRQELITFVADRPGHDHRYAID
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEE
FTKLNAELKWSPRESFTTGLRRTINWYLDNPGWIDRIRSGAYAAWIDEQYGDSSVSQTAA
EEEECCEEEECCHHHHHHHHHHEEEEEECCCHHHHHHHCCCEEEEECHHCCCCHHHHHHC
VEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]