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The map label for this gene is cca [H]

Identifier: 85859113

GI number: 85859113

Start: 1290237

End: 1291724

Strand: Direct

Name: cca [H]

Synonym: SYN_01518

Alternate gene names: 85859113

Gene position: 1290237-1291724 (Clockwise)

Preceding gene: 85859112

Following gene: 85859114

Centisome position: 40.58

GC content: 61.56

Gene sequence:

>1488_bases
ATGGGCAGATCGACCTGTCCGTCGATCCGGACCGGAGCCGGGCACGGCTGGAGCGCAGCGAAACGGCCAAAGAGGAAGGC
TGTACCATGTGCGGCGATCTGTGCGCCATCAAGCTCGGGAAAAAGAAAGCCCCCTGTTAGACGGATGGCCTGCCCCATGA
CGATCCGCGATCCGGGAGAACGTACCGAATTGGTCTCGCGCCGGGATATCGCCGCCGGGATCGTCCGCCGTTTGAAGGAA
GCGGGGCATGAAGCCTGGTTTGTCGGCGGCTGCGTCCGGGATTATCTGCGGGGCGTGGAAGCCGGTGATTACGACATCGT
CACCTCGGCCTCTCCCGAAGCGATTCAGGCGCTTTTTCCCCGAACGGTCGGAGTGGGCATCCGCTTCGGGATTCTCATCG
TGATCGAGAACGGCTATTCCCATGAAGTAGCCACCTACCGCTCCCCCGGCGGTTATCTGGAAGACGTTTTGCGGCGGGAT
TTCACCATCAACGCCATGCTCCAGGACCCCGATACCGGCCGGATTATAGATGAAGTCGGCGGACGGGCCGATCTGGAAGG
CCGCGTCATCCGCGCCGTGGGCCGCCCCGATGAACGATTCGCCGAGGACCGTCTGCGCATGCTCAGGGCCGTCCGTTTTG
CCGCAAACCTGGCTTTTTCGGTCGAGCAGGAGACGCTTGAAGCCATTCAGGCCCATGCCCCGGAGATCCAGGAAGTCAGT
GTCGAACGCGTGGCCGATGAACTGACCCGCCTGCTGACCCTCGGCGGGGCGGGGGAGGGGATGCGACTTTTGATGAAGAC
AGGCCTGCTGGAGGAACTCCTCCCCGAGGTGGCGGCCATGCGGGGCGTGAAGCAGCCCCCGAAGTTCCATCCCGAGGGAG
ATGTCTGGACCCACACCGTCAAGATGCTCGATCTGATGAGCATCCCCGCCGATTCCCGACTGGCCTGGGGCGTTCTCCTG
CACGACGTGGGCAAGCCGGATGTGCAGAGCAGGGATCATCGGGGGATTCATTTCTACGGGCACGTTCAGCAGGGTGAGAT
CCTGGCGGAAGGCATCCTCCGCCGCCTGCGTTTTCCCCGTGCCGATATGGAGTGCATCCTGGGGTTGATCCGCTGTCACA
TGAGCTTCATCAACGTCCGGGAGATGAGGACGAGCCGTCTGAAACGCCTGCTCCGGAGTCCCGATTTCGATCTGTTACTG
GAGCTGCACCGCCTCGATTGCCTGGGCAGCAACGGGGTGCTGGATTCCTATGAGTATTGCCTGCAAAAACAGCGGGAACT
GACCGACGAGCAGTTGAGGCCGCCTCCTCTGCTGAGGGGAAAGGACCTGCTGGAGATGGGATATTTACCAGGCGCCCTTT
TCCGGGAGATCCTGAACGCCGTGGAGGATGCCCAGCTCAACGGATCGATTGCCACACCCGAAGAGGCCCGCCAACTGGTG
AAAAACCGCTGGCCGCACAGGAGAATTCAAATTTCAGATCAGAGTTGA

Upstream 100 bases:

>100_bases
TCCCGGATCGCCGCCCACATTGCGGACATCGCCAAGGGATATCCCGGGGCGATGGAAAAGGATATCCTGATGGCGAAATA
CCGCAGTGAATTCAACTGGA

Downstream 100 bases:

>100_bases
ATTCAAAGCGGCAAGGCAGAGAGGCGCAGGCCGCGCAGCAGAATGTTGAGGATTCGATAACCAGCCAGGCGAAGAGCGCG
CCTTGAAGTGAAATAGAAAT

Product: poly A polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MGRSTCPSIRTGAGHGWSAAKRPKRKAVPCAAICAPSSSGKRKPPVRRMACPMTIRDPGERTELVSRRDIAAGIVRRLKE
AGHEAWFVGGCVRDYLRGVEAGDYDIVTSASPEAIQALFPRTVGVGIRFGILIVIENGYSHEVATYRSPGGYLEDVLRRD
FTINAMLQDPDTGRIIDEVGGRADLEGRVIRAVGRPDERFAEDRLRMLRAVRFAANLAFSVEQETLEAIQAHAPEIQEVS
VERVADELTRLLTLGGAGEGMRLLMKTGLLEELLPEVAAMRGVKQPPKFHPEGDVWTHTVKMLDLMSIPADSRLAWGVLL
HDVGKPDVQSRDHRGIHFYGHVQQGEILAEGILRRLRFPRADMECILGLIRCHMSFINVREMRTSRLKRLLRSPDFDLLL
ELHRLDCLGSNGVLDSYEYCLQKQRELTDEQLRPPPLLRGKDLLEMGYLPGALFREILNAVEDAQLNGSIATPEEARQLV
KNRWPHRRIQISDQS

Sequences:

>Translated_495_residues
MGRSTCPSIRTGAGHGWSAAKRPKRKAVPCAAICAPSSSGKRKPPVRRMACPMTIRDPGERTELVSRRDIAAGIVRRLKE
AGHEAWFVGGCVRDYLRGVEAGDYDIVTSASPEAIQALFPRTVGVGIRFGILIVIENGYSHEVATYRSPGGYLEDVLRRD
FTINAMLQDPDTGRIIDEVGGRADLEGRVIRAVGRPDERFAEDRLRMLRAVRFAANLAFSVEQETLEAIQAHAPEIQEVS
VERVADELTRLLTLGGAGEGMRLLMKTGLLEELLPEVAAMRGVKQPPKFHPEGDVWTHTVKMLDLMSIPADSRLAWGVLL
HDVGKPDVQSRDHRGIHFYGHVQQGEILAEGILRRLRFPRADMECILGLIRCHMSFINVREMRTSRLKRLLRSPDFDLLL
ELHRLDCLGSNGVLDSYEYCLQKQRELTDEQLRPPPLLRGKDLLEMGYLPGALFREILNAVEDAQLNGSIATPEEARQLV
KNRWPHRRIQISDQS
>Mature_494_residues
GRSTCPSIRTGAGHGWSAAKRPKRKAVPCAAICAPSSSGKRKPPVRRMACPMTIRDPGERTELVSRRDIAAGIVRRLKEA
GHEAWFVGGCVRDYLRGVEAGDYDIVTSASPEAIQALFPRTVGVGIRFGILIVIENGYSHEVATYRSPGGYLEDVLRRDF
TINAMLQDPDTGRIIDEVGGRADLEGRVIRAVGRPDERFAEDRLRMLRAVRFAANLAFSVEQETLEAIQAHAPEIQEVSV
ERVADELTRLLTLGGAGEGMRLLMKTGLLEELLPEVAAMRGVKQPPKFHPEGDVWTHTVKMLDLMSIPADSRLAWGVLLH
DVGKPDVQSRDHRGIHFYGHVQQGEILAEGILRRLRFPRADMECILGLIRCHMSFINVREMRTSRLKRLLRSPDFDLLLE
LHRLDCLGSNGVLDSYEYCLQKQRELTDEQLRPPPLLRGKDLLEMGYLPGALFREILNAVEDAQLNGSIATPEEARQLVK
NRWPHRRIQISDQS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=193, Percent_Identity=32.6424870466321, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI87081691, Length=277, Percent_Identity=34.6570397111913, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789436, Length=303, Percent_Identity=35.6435643564356, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281360112, Length=274, Percent_Identity=29.5620437956204, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21357337, Length=274, Percent_Identity=29.5620437956204, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 55416; Mature: 55285

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRSTCPSIRTGAGHGWSAAKRPKRKAVPCAAICAPSSSGKRKPPVRRMACPMTIRDPGE
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCH
RTELVSRRDIAAGIVRRLKEAGHEAWFVGGCVRDYLRGVEAGDYDIVTSASPEAIQALFP
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
RTVGVGIRFGILIVIENGYSHEVATYRSPGGYLEDVLRRDFTINAMLQDPDTGRIIDEVG
HHHCCCEEEEEEEEEECCCCCCCCEECCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHCC
GRADLEGRVIRAVGRPDERFAEDRLRMLRAVRFAANLAFSVEQETLEAIQAHAPEIQEVS
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VERVADELTRLLTLGGAGEGMRLLMKTGLLEELLPEVAAMRGVKQPPKFHPEGDVWTHTV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
KMLDLMSIPADSRLAWGVLLHDVGKPDVQSRDHRGIHFYGHVQQGEILAEGILRRLRFPR
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCC
ADMECILGLIRCHMSFINVREMRTSRLKRLLRSPDFDLLLELHRLDCLGSNGVLDSYEYC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHH
LQKQRELTDEQLRPPPLLRGKDLLEMGYLPGALFREILNAVEDAQLNGSIATPEEARQLV
HHHHHHCCHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KNRWPHRRIQISDQS
HCCCCCCEEEECCCC
>Mature Secondary Structure 
GRSTCPSIRTGAGHGWSAAKRPKRKAVPCAAICAPSSSGKRKPPVRRMACPMTIRDPGE
CCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCH
RTELVSRRDIAAGIVRRLKEAGHEAWFVGGCVRDYLRGVEAGDYDIVTSASPEAIQALFP
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
RTVGVGIRFGILIVIENGYSHEVATYRSPGGYLEDVLRRDFTINAMLQDPDTGRIIDEVG
HHHCCCEEEEEEEEEECCCCCCCCEECCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHCC
GRADLEGRVIRAVGRPDERFAEDRLRMLRAVRFAANLAFSVEQETLEAIQAHAPEIQEVS
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VERVADELTRLLTLGGAGEGMRLLMKTGLLEELLPEVAAMRGVKQPPKFHPEGDVWTHTV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
KMLDLMSIPADSRLAWGVLLHDVGKPDVQSRDHRGIHFYGHVQQGEILAEGILRRLRFPR
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCC
ADMECILGLIRCHMSFINVREMRTSRLKRLLRSPDFDLLLELHRLDCLGSNGVLDSYEYC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHH
LQKQRELTDEQLRPPPLLRGKDLLEMGYLPGALFREILNAVEDAQLNGSIATPEEARQLV
HHHHHHCCHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KNRWPHRRIQISDQS
HCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA