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The map label for this gene is kinA [H]

Identifier: 85858812

GI number: 85858812

Start: 1002795

End: 1004042

Strand: Direct

Name: kinA [H]

Synonym: SYN_01465

Alternate gene names: 85858812

Gene position: 1002795-1004042 (Clockwise)

Preceding gene: 85858811

Following gene: 85858813

Centisome position: 31.54

GC content: 43.43

Gene sequence:

>1248_bases
ATGAATGACATTGCATCGTTACAGAAATCTTTCGAAGGGTTCAATCAGGCGACTTTGCATATTCAGCAGGCTTTTGCGAG
TCTGGAGAAGAAATTTGACAGCCTGAATCTTGAACTGGAAGAAAAGAACCGGGAGTTGTTGAGAACGCTTTCCGAGAAGG
AGGAAATGAAGAGTTACCTTCAGAATATTCTCGAAAGTTTGACGAACGGAGTCATTGTGACCAATCCGGAGGATGAAATT
CAGGTGTTCAATTCCGCCTCGGAATTATTTTCAGGCAAGCCACAGGATGAAGCTGTCGGGAGGCACATCCGGATTTTATT
TGAAGATATGCCTTCAAATGACTGGATGGACATTTTTTTGAACAAAAATTTCCGGAAGGGGAGGGTTTCCCGCCTGATTT
ATAAGAATCGGGTTCTTGAAATTACGTCATCGTTTATTTCGTCGGGAGGTAAAATTCTCGGAACGGTTTTCGTTCTCCGG
GACGTCACCCGCATTGAAAAACTGGAAAGCATGGCAAAAAGATCCGAAAAATTTACCGCTTTGGGGGAAATGGCGGCGAA
TATCGCTCATGAAATTAGAAATCCCCTGGGAAGCATTGAACTGTACGCATCCCTTTTGATGAAGGAATCGAAGAACGATA
GAGATCAGAACAGAATTTCCCGGATTATCTCATCCGTCAAGAAAATGGATAATAAAATATCCAACCTTCTTCTCTTCACG
AAAAACCGAAAACTGATCTTAAAACGGATTTCATTACACCATTTTTTGAACGAGATCCTCGGGTCATGCGAACCGCTGAT
TGACCAGGAGAAGATATTCATTAATACAACTTACGCAGATTATGAACCGTGGATCGAGGGTGATGCGGAAATGCTGAAGC
AAGTCTTTTTCAACCTGATCCTCAACGCGTTGCAGTCTCTGGATCCTGAGGGAGGGACTATCGATTTTGAGACAATCTGT
CCTCACTCAGGGCAGGATATGGAAACGGATGAATCTTATATTGAGGTTAAAATCAGAGATTCGGGAATCGGAATTCCCAG
GGAATATTTAAACCGGATCTTTGATCCCTTTTTTACGACTAAGGAAGAGGAATCGGGATTGGGACTGACGATTGTTCACA
ATATTATCGAAATGCACCGCGGGGTAATCCATGTGGAAAGCGTCGAACAGGAGGGAACTGTTTTTCACGTTCTCCTCCCG
TTGATCAGCGAACAAGGCACAATGAAGACGAAGGTAGGATGGAAATGA

Upstream 100 bases:

>100_bases
ACATTCTGGCCGAAGGTCGTGGATTACTGGGTTGCCGATCAGGCGTGGTCTGAAAACAACATAACGTACATGAGTAGTAA
TTAAAATTACTGGATCAAGC

Downstream 100 bases:

>100_bases
AAGAAGCGCGCATACTGGTCGTGGATGATGACACGTCAATGAGAATGGCACTCTGCGAAACTCTGGAAAGCTGTGGCTAC
TGCATTGAAACAGCTTCCAA

Product: signal transduction histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNDIASLQKSFEGFNQATLHIQQAFASLEKKFDSLNLELEEKNRELLRTLSEKEEMKSYLQNILESLTNGVIVTNPEDEI
QVFNSASELFSGKPQDEAVGRHIRILFEDMPSNDWMDIFLNKNFRKGRVSRLIYKNRVLEITSSFISSGGKILGTVFVLR
DVTRIEKLESMAKRSEKFTALGEMAANIAHEIRNPLGSIELYASLLMKESKNDRDQNRISRIISSVKKMDNKISNLLLFT
KNRKLILKRISLHHFLNEILGSCEPLIDQEKIFINTTYADYEPWIEGDAEMLKQVFFNLILNALQSLDPEGGTIDFETIC
PHSGQDMETDESYIEVKIRDSGIGIPREYLNRIFDPFFTTKEEESGLGLTIVHNIIEMHRGVIHVESVEQEGTVFHVLLP
LISEQGTMKTKVGWK

Sequences:

>Translated_415_residues
MNDIASLQKSFEGFNQATLHIQQAFASLEKKFDSLNLELEEKNRELLRTLSEKEEMKSYLQNILESLTNGVIVTNPEDEI
QVFNSASELFSGKPQDEAVGRHIRILFEDMPSNDWMDIFLNKNFRKGRVSRLIYKNRVLEITSSFISSGGKILGTVFVLR
DVTRIEKLESMAKRSEKFTALGEMAANIAHEIRNPLGSIELYASLLMKESKNDRDQNRISRIISSVKKMDNKISNLLLFT
KNRKLILKRISLHHFLNEILGSCEPLIDQEKIFINTTYADYEPWIEGDAEMLKQVFFNLILNALQSLDPEGGTIDFETIC
PHSGQDMETDESYIEVKIRDSGIGIPREYLNRIFDPFFTTKEEESGLGLTIVHNIIEMHRGVIHVESVEQEGTVFHVLLP
LISEQGTMKTKVGWK
>Mature_415_residues
MNDIASLQKSFEGFNQATLHIQQAFASLEKKFDSLNLELEEKNRELLRTLSEKEEMKSYLQNILESLTNGVIVTNPEDEI
QVFNSASELFSGKPQDEAVGRHIRILFEDMPSNDWMDIFLNKNFRKGRVSRLIYKNRVLEITSSFISSGGKILGTVFVLR
DVTRIEKLESMAKRSEKFTALGEMAANIAHEIRNPLGSIELYASLLMKESKNDRDQNRISRIISSVKKMDNKISNLLLFT
KNRKLILKRISLHHFLNEILGSCEPLIDQEKIFINTTYADYEPWIEGDAEMLKQVFFNLILNALQSLDPEGGTIDFETIC
PHSGQDMETDESYIEVKIRDSGIGIPREYLNRIFDPFFTTKEEESGLGLTIVHNIIEMHRGVIHVESVEQEGTVFHVLLP
LISEQGTMKTKVGWK

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=29.4117647058824, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=34.468085106383, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693157, Length=277, Percent_Identity=28.8808664259928, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790300, Length=230, Percent_Identity=30.8695652173913, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790346, Length=238, Percent_Identity=25.2100840336134, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786783, Length=247, Percent_Identity=26.7206477732794, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994928, Length=439, Percent_Identity=22.0956719817768, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790861, Length=240, Percent_Identity=24.1666666666667, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790567, Length=361, Percent_Identity=26.5927977839335, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=258, Percent_Identity=23.6434108527132, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789149, Length=241, Percent_Identity=26.5560165975104, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081816, Length=246, Percent_Identity=26.8292682926829, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47538; Mature: 47538

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIASLQKSFEGFNQATLHIQQAFASLEKKFDSLNLELEEKNRELLRTLSEKEEMKSYL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHH
QNILESLTNGVIVTNPEDEIQVFNSASELFSGKPQDEAVGRHIRILFEDMPSNDWMDIFL
HHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEECCCCCCCEEEEE
NKNFRKGRVSRLIYKNRVLEITSSFISSGGKILGTVFVLRDVTRIEKLESMAKRSEKFTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGEMAANIAHEIRNPLGSIELYASLLMKESKNDRDQNRISRIISSVKKMDNKISNLLLFT
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEE
KNRKLILKRISLHHFLNEILGSCEPLIDQEKIFINTTYADYEPWIEGDAEMLKQVFFNLI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
LNALQSLDPEGGTIDFETICPHSGQDMETDESYIEVKIRDSGIGIPREYLNRIFDPFFTT
HHHHHHCCCCCCEEEEHCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCC
KEEESGLGLTIVHNIIEMHRGVIHVESVEQEGTVFHVLLPLISEQGTMKTKVGWK
CCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNDIASLQKSFEGFNQATLHIQQAFASLEKKFDSLNLELEEKNRELLRTLSEKEEMKSYL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHH
QNILESLTNGVIVTNPEDEIQVFNSASELFSGKPQDEAVGRHIRILFEDMPSNDWMDIFL
HHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEECCCCCCCEEEEE
NKNFRKGRVSRLIYKNRVLEITSSFISSGGKILGTVFVLRDVTRIEKLESMAKRSEKFTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGEMAANIAHEIRNPLGSIELYASLLMKESKNDRDQNRISRIISSVKKMDNKISNLLLFT
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEE
KNRKLILKRISLHHFLNEILGSCEPLIDQEKIFINTTYADYEPWIEGDAEMLKQVFFNLI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
LNALQSLDPEGGTIDFETICPHSGQDMETDESYIEVKIRDSGIGIPREYLNRIFDPFFTT
HHHHHHCCCCCCEEEEHCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCC
KEEESGLGLTIVHNIIEMHRGVIHVESVEQEGTVFHVLLPLISEQGTMKTKVGWK
CCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]