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The map label for this gene is tolB [H]

Identifier: 85858720

GI number: 85858720

Start: 898678

End: 900168

Strand: Direct

Name: tolB [H]

Synonym: SYN_00188

Alternate gene names: 85858720

Gene position: 898678-900168 (Clockwise)

Preceding gene: 85858719

Following gene: 85858721

Centisome position: 28.27

GC content: 56.54

Gene sequence:

>1491_bases
ATGGATCACACGACAGAAGACCGGCACCGAAGCTGGCGACGAAAAGCGACTTTGGATCAGTCGCAGGAAACGGGGAAAAG
CATGATTCCAGAGAAAGAAAAGGCGGGGGAGCAGAGGAAGCGGCCGGATGGAATGTGCGGTGTAAGACGCGGGATGGGGG
TGCTGCTCCTTTTCTGCGCGGTCGCTTTGTGCGCTATGCCCTTTGTCGTTCGATCCGTCTGGGGGAAGGTTTATATCGAC
ATCGATTCCCCGGCGTTTCAGAAAATCCCGATTGCCGTTGCCGATTTTGCGCCTCTGAACGGCAATCAGGGCCATGCGGA
TCTCTCGTCCTGGTTTCCCGGCGCTGTGCGCAAAACCCTTGATCTGACCGGGTATTTCAATATTCTGAACAGGACGGGCG
GACCGCGTGATCAAAGTCAGACCGGAGCAGTCCAAGCGCAGGCGGCCTACGGGGAATGGCGAAGTCTTGGTGCGGAATAT
CTGATTCAAGGCGGCTTCTCTTCCCGGGGGACACAGCTGGTCGCGGAATTCCGCCTTATCGACATTGTGCAGGGGCGTCA
GCTGATGGGAAAGCAGTATACCGGAGGATTTGAAGACCGCAGGGACATGGTGATCCGCTTTGTTCAGGAAGTCCTTTCGC
TTCTGACCGGTGGGGAAGGTTTCTTTGATACGCGGATCGCCTTTGTTGTCCGTCAGGGTCGTTCTTCAAGCCTCCACACT
GTAGGGTTCGGTTCGCAGATCGACGGCCGGGACCTTGCCCGGGTAGCCGGCAGTCCTTCCCTGATCCTTTCACCGCGCTG
GTCTCCTGATGGACGTTATCTTGCTTTTTCTTCCTATCGCGACGGGAAACCGGATATCTTTGTCGTATCACCCTCCGGCA
GCGGTTTGAAGAAAATAGTCAGTTTCCAGGGACTCAATCTTCCCGGAGCCTGGTCTCCCGATGGACGGAGTCTGCTTCTG
ACCCTGAGCAAGGACGGAAATGAAGAAATCTACGTCATGGATGTGCGCAGCGGTCAGGTGCGTCGACTGACCCGCAATTC
AGCGATTGACGTCTCTCCCGTCTGGTCTCCCGATGGGCGCAGGATCGCCTTTGTCTCCAACATTTCCGGTTCGCCGCAGA
TTTATGTCATGAACGCGGATGGCGGGGATGTCCGTCGACTGACCTATTCGGGAAATTACAACACAACTCCTGCCTGGTCG
CCCCGGGGGGGGAAGATTGCCTATGAGGGGAAGGTCGGGAGCGGTTATCAGATTTTCTCGATTGACGAGGATGGCGGCAA
TGTCCGGCAATTGACCTCCGGAGCGGGCGATCATGAATTTCCTTCCTGGTCTCCCGACGGTCGATTCCTGACATTCAGCC
TCCGCAGCGGCGGCCGCAGTCGGATCAATATTCTCAATGCCAATACGCTGGAAGTGCGGACTCTTTATGAAAGTACCGAT
CGTTGCCTGGGGCCCGCCTGGTCGCCCAGGCTGAAACAGGCTTTAAGATAG

Upstream 100 bases:

>100_bases
AATGGGCATCCGTTTTCATTCATCCGAACTGAGGTAGCCAACACCAGACACAATACTGCAAGAGGGATAAAGGGCGGCAG
AAAGAAAGCGATGCTGCCGC

Downstream 100 bases:

>100_bases
GAATGCTGCCGAGGTTTTCACGGCAACAGGGCAGGGTTGGTATGTAAAACATTGATTTGTCTAAAAATAAAAAATATTTG
GAATTTAGGGTTGACCTTCT

Product: tolB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MDHTTEDRHRSWRRKATLDQSQETGKSMIPEKEKAGEQRKRPDGMCGVRRGMGVLLLFCAVALCAMPFVVRSVWGKVYID
IDSPAFQKIPIAVADFAPLNGNQGHADLSSWFPGAVRKTLDLTGYFNILNRTGGPRDQSQTGAVQAQAAYGEWRSLGAEY
LIQGGFSSRGTQLVAEFRLIDIVQGRQLMGKQYTGGFEDRRDMVIRFVQEVLSLLTGGEGFFDTRIAFVVRQGRSSSLHT
VGFGSQIDGRDLARVAGSPSLILSPRWSPDGRYLAFSSYRDGKPDIFVVSPSGSGLKKIVSFQGLNLPGAWSPDGRSLLL
TLSKDGNEEIYVMDVRSGQVRRLTRNSAIDVSPVWSPDGRRIAFVSNISGSPQIYVMNADGGDVRRLTYSGNYNTTPAWS
PRGGKIAYEGKVGSGYQIFSIDEDGGNVRQLTSGAGDHEFPSWSPDGRFLTFSLRSGGRSRINILNANTLEVRTLYESTD
RCLGPAWSPRLKQALR

Sequences:

>Translated_496_residues
MDHTTEDRHRSWRRKATLDQSQETGKSMIPEKEKAGEQRKRPDGMCGVRRGMGVLLLFCAVALCAMPFVVRSVWGKVYID
IDSPAFQKIPIAVADFAPLNGNQGHADLSSWFPGAVRKTLDLTGYFNILNRTGGPRDQSQTGAVQAQAAYGEWRSLGAEY
LIQGGFSSRGTQLVAEFRLIDIVQGRQLMGKQYTGGFEDRRDMVIRFVQEVLSLLTGGEGFFDTRIAFVVRQGRSSSLHT
VGFGSQIDGRDLARVAGSPSLILSPRWSPDGRYLAFSSYRDGKPDIFVVSPSGSGLKKIVSFQGLNLPGAWSPDGRSLLL
TLSKDGNEEIYVMDVRSGQVRRLTRNSAIDVSPVWSPDGRRIAFVSNISGSPQIYVMNADGGDVRRLTYSGNYNTTPAWS
PRGGKIAYEGKVGSGYQIFSIDEDGGNVRQLTSGAGDHEFPSWSPDGRFLTFSLRSGGRSRINILNANTLEVRTLYESTD
RCLGPAWSPRLKQALR
>Mature_496_residues
MDHTTEDRHRSWRRKATLDQSQETGKSMIPEKEKAGEQRKRPDGMCGVRRGMGVLLLFCAVALCAMPFVVRSVWGKVYID
IDSPAFQKIPIAVADFAPLNGNQGHADLSSWFPGAVRKTLDLTGYFNILNRTGGPRDQSQTGAVQAQAAYGEWRSLGAEY
LIQGGFSSRGTQLVAEFRLIDIVQGRQLMGKQYTGGFEDRRDMVIRFVQEVLSLLTGGEGFFDTRIAFVVRQGRSSSLHT
VGFGSQIDGRDLARVAGSPSLILSPRWSPDGRYLAFSSYRDGKPDIFVVSPSGSGLKKIVSFQGLNLPGAWSPDGRSLLL
TLSKDGNEEIYVMDVRSGQVRRLTRNSAIDVSPVWSPDGRRIAFVSNISGSPQIYVMNADGGDVRRLTYSGNYNTTPAWS
PRGGKIAYEGKVGSGYQIFSIDEDGGNVRQLTSGAGDHEFPSWSPDGRFLTFSLRSGGRSRINILNANTLEVRTLYESTD
RCLGPAWSPRLKQALR

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=448, Percent_Identity=30.1339285714286, Blast_Score=161, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI32564215, Length=215, Percent_Identity=21.8604651162791, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32564217, Length=215, Percent_Identity=21.8604651162791, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 54556; Mature: 54556

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHTTEDRHRSWRRKATLDQSQETGKSMIPEKEKAGEQRKRPDGMCGVRRGMGVLLLFCA
CCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VALCAMPFVVRSVWGKVYIDIDSPAFQKIPIAVADFAPLNGNQGHADLSSWFPGAVRKTL
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCHHHHHHH
DLTGYFNILNRTGGPRDQSQTGAVQAQAAYGEWRSLGAEYLIQGGFSSRGTQLVAEFRLI
HHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHCCHHHEEECCCCCCCHHHHHHHHHH
DIVQGRQLMGKQYTGGFEDRRDMVIRFVQEVLSLLTGGEGFFDTRIAFVVRQGRSSSLHT
HHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCCCCEEE
VGFGSQIDGRDLARVAGSPSLILSPRWSPDGRYLAFSSYRDGKPDIFVVSPSGSGLKKIV
EECCCCCCCCHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHH
SFQGLNLPGAWSPDGRSLLLTLSKDGNEEIYVMDVRSGQVRRLTRNSAIDVSPVWSPDGR
HHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCEEEECCCCCEEECCCCCCCCC
RIAFVSNISGSPQIYVMNADGGDVRRLTYSGNYNTTPAWSPRGGKIAYEGKVGSGYQIFS
EEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEE
IDEDGGNVRQLTSGAGDHEFPSWSPDGRFLTFSLRSGGRSRINILNANTLEVRTLYESTD
EECCCCCEEEHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCEEEEEEHHHHHH
RCLGPAWSPRLKQALR
HHCCCCCCHHHHHHCC
>Mature Secondary Structure
MDHTTEDRHRSWRRKATLDQSQETGKSMIPEKEKAGEQRKRPDGMCGVRRGMGVLLLFCA
CCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VALCAMPFVVRSVWGKVYIDIDSPAFQKIPIAVADFAPLNGNQGHADLSSWFPGAVRKTL
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCHHHHHHH
DLTGYFNILNRTGGPRDQSQTGAVQAQAAYGEWRSLGAEYLIQGGFSSRGTQLVAEFRLI
HHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHCCHHHEEECCCCCCCHHHHHHHHHH
DIVQGRQLMGKQYTGGFEDRRDMVIRFVQEVLSLLTGGEGFFDTRIAFVVRQGRSSSLHT
HHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCCCCEEE
VGFGSQIDGRDLARVAGSPSLILSPRWSPDGRYLAFSSYRDGKPDIFVVSPSGSGLKKIV
EECCCCCCCCHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHH
SFQGLNLPGAWSPDGRSLLLTLSKDGNEEIYVMDVRSGQVRRLTRNSAIDVSPVWSPDGR
HHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCEEEECCCCCEEECCCCCCCCC
RIAFVSNISGSPQIYVMNADGGDVRRLTYSGNYNTTPAWSPRGGKIAYEGKVGSGYQIFS
EEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEE
IDEDGGNVRQLTSGAGDHEFPSWSPDGRFLTFSLRSGGRSRINILNANTLEVRTLYESTD
EECCCCCEEEHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCEEEEEEHHHHHH
RCLGPAWSPRLKQALR
HHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA