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The map label for this gene is hemB [H]

Identifier: 85858424

GI number: 85858424

Start: 575279

End: 576253

Strand: Direct

Name: hemB [H]

Synonym: SYN_02267

Alternate gene names: 85858424

Gene position: 575279-576253 (Clockwise)

Preceding gene: 85858423

Following gene: 85858425

Centisome position: 18.09

GC content: 57.95

Gene sequence:

>975_bases
ATGAATTTCCCCGAGTATCGACCCCGGCGACTCCGGAAGAACGATCTTTTCCGGCGGCTCATCCGGGAAACCCGTCTGTC
TGTGGATTCCTTCATTTTTCCCCTGTTTGCAACGGTCGGCAAGGGAGTGAAAAAACCCATCGATTCCATGCCGGGTCATT
TCCAGCTATCCGTTGAGCTGCTTGTAAAAGAGGTTCAGGAATGTCGGGAACTGGGAATCCCGGCAGTGTTGCTTTTCGGG
ATTCCGGAGAGGAAGGACGAAGCGGCTTCCGGGGCCTTTGCCCGGGATGGAATTGTGCAGCAGGCTGTCCGGCGGATCAA
GGACAAGGTTCCGGATATTCTCGTGGTGACGGATGTCTGCCTCTGCGAATACACAGACCACGGCCACTGCGGGATGATTG
AAAAGGGAGAGGTTCACAACGACATGACCCTGGAGGTGCTGGCGGAAACGGCCGTCTCTCATGCGAAGGCGGGGGCGGAT
ATGGTGGCCCCCTCGGCCATGATGGACGGTCAGGTCGGGGCGATCCGCGAAGGGCTGGATGAGGCGGGATTTGAAACGGT
TCCCATTCTGGCCTATTCGGCGAAGTATGCCTCCTGCTTTTACGGCCCCTTCCGGGAGGCGGCGGAAAGCGCCTCCCGCT
TTGGCGACCGGAAGGCCTATCAGATGGATCCGGCCAACAGTGATGAAGCCGTCCGGGAGATGAACCTGGATGTTCAGGAA
GGCGCCGACATCCTCATGGTGAAGCCGGCCCTGCCTTATCTCGATGTCATTCGTCGCGCGAAGGAAGAATTCGATCTGCC
CGTTGCGGCCTATAATGTCAGCGGCGAATTCGCCATGATCAAGGCCGCTGCCCGACTGGGCTGGATTGATGAAGAAAAAG
CCATGATGGAATCTTTGACGGCCATCCGCCGTGCCGGTGCGGACATCATTCTGACCTATTTCGCTCGGGAAGCGGCGAAA
GTTCTCCAGCGATGA

Upstream 100 bases:

>100_bases
AAACGGCCTTTGCATGAGACATGCAACCGTAGTTATATATTGAGGAAGTGTGTTTTGATGAAAACGTATCAGGAAAGATC
CCAAGCGAGGTGAAAACCCT

Downstream 100 bases:

>100_bases
ACAGTTCTCCGCTCCCTTCAACTCTGCGAATGGTGGCCTGGGAAGTGACCCGGAGCTGCAACCTGTCCTGCATTCACTGC
CGGGCCTCGGCGGAGAAAGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNFPEYRPRRLRKNDLFRRLIRETRLSVDSFIFPLFATVGKGVKKPIDSMPGHFQLSVELLVKEVQECRELGIPAVLLFG
IPERKDEAASGAFARDGIVQQAVRRIKDKVPDILVVTDVCLCEYTDHGHCGMIEKGEVHNDMTLEVLAETAVSHAKAGAD
MVAPSAMMDGQVGAIREGLDEAGFETVPILAYSAKYASCFYGPFREAAESASRFGDRKAYQMDPANSDEAVREMNLDVQE
GADILMVKPALPYLDVIRRAKEEFDLPVAAYNVSGEFAMIKAAARLGWIDEEKAMMESLTAIRRAGADIILTYFAREAAK
VLQR

Sequences:

>Translated_324_residues
MNFPEYRPRRLRKNDLFRRLIRETRLSVDSFIFPLFATVGKGVKKPIDSMPGHFQLSVELLVKEVQECRELGIPAVLLFG
IPERKDEAASGAFARDGIVQQAVRRIKDKVPDILVVTDVCLCEYTDHGHCGMIEKGEVHNDMTLEVLAETAVSHAKAGAD
MVAPSAMMDGQVGAIREGLDEAGFETVPILAYSAKYASCFYGPFREAAESASRFGDRKAYQMDPANSDEAVREMNLDVQE
GADILMVKPALPYLDVIRRAKEEFDLPVAAYNVSGEFAMIKAAARLGWIDEEKAMMESLTAIRRAGADIILTYFAREAAK
VLQR
>Mature_324_residues
MNFPEYRPRRLRKNDLFRRLIRETRLSVDSFIFPLFATVGKGVKKPIDSMPGHFQLSVELLVKEVQECRELGIPAVLLFG
IPERKDEAASGAFARDGIVQQAVRRIKDKVPDILVVTDVCLCEYTDHGHCGMIEKGEVHNDMTLEVLAETAVSHAKAGAD
MVAPSAMMDGQVGAIREGLDEAGFETVPILAYSAKYASCFYGPFREAAESASRFGDRKAYQMDPANSDEAVREMNLDVQE
GADILMVKPALPYLDVIRRAKEEFDLPVAAYNVSGEFAMIKAAARLGWIDEEKAMMESLTAIRRAGADIILTYFAREAAK
VLQR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=327, Percent_Identity=43.7308868501529, Blast_Score=271, Evalue=8e-73,
Organism=Escherichia coli, GI87081728, Length=314, Percent_Identity=50.9554140127389, Blast_Score=306, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6321398, Length=311, Percent_Identity=41.1575562700965, Blast_Score=224, Evalue=1e-59,
Organism=Drosophila melanogaster, GI21358291, Length=288, Percent_Identity=43.75, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35983; Mature: 35983

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPEYRPRRLRKNDLFRRLIRETRLSVDSFIFPLFATVGKGVKKPIDSMPGHFQLSVEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHH
LVKEVQECRELGIPAVLLFGIPERKDEAASGAFARDGIVQQAVRRIKDKVPDILVVTDVC
HHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHH
LCEYTDHGHCGMIEKGEVHNDMTLEVLAETAVSHAKAGADMVAPSAMMDGQVGAIREGLD
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCHHHHHHHHH
EAGFETVPILAYSAKYASCFYGPFREAAESASRFGDRKAYQMDPANSDEAVREMNLDVQE
HCCCCEEHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHCCCHHH
GADILMVKPALPYLDVIRRAKEEFDLPVAAYNVSGEFAMIKAAARLGWIDEEKAMMESLT
CCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHH
AIRRAGADIILTYFAREAAKVLQR
HHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFPEYRPRRLRKNDLFRRLIRETRLSVDSFIFPLFATVGKGVKKPIDSMPGHFQLSVEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHH
LVKEVQECRELGIPAVLLFGIPERKDEAASGAFARDGIVQQAVRRIKDKVPDILVVTDVC
HHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHH
LCEYTDHGHCGMIEKGEVHNDMTLEVLAETAVSHAKAGADMVAPSAMMDGQVGAIREGLD
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCHHHHHHHHH
EAGFETVPILAYSAKYASCFYGPFREAAESASRFGDRKAYQMDPANSDEAVREMNLDVQE
HCCCCEEHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHCCCHHH
GADILMVKPALPYLDVIRRAKEEFDLPVAAYNVSGEFAMIKAAARLGWIDEEKAMMESLT
CCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHH
AIRRAGADIILTYFAREAAKVLQR
HHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8123787 [H]