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The map label for this gene is pqqE [H]

Identifier: 85858425

GI number: 85858425

Start: 576250

End: 577359

Strand: Direct

Name: pqqE [H]

Synonym: SYN_02266

Alternate gene names: 85858425

Gene position: 576250-577359 (Clockwise)

Preceding gene: 85858424

Following gene: 85858426

Centisome position: 18.13

GC content: 59.19

Gene sequence:

>1110_bases
ATGAACAGTTCTCCGCTCCCTTCAACTCTGCGAATGGTGGCCTGGGAAGTGACCCGGAGCTGCAACCTGTCCTGCATTCA
CTGCCGGGCCTCGGCGGAGAAAGGTCCCTATGCCGGGGAACTCTCCACCGAAGAGTCGAAAAACCTTCTGGAGGAGATCG
CCGCGTTCAGCAGGCCGGTGGTCATCCTGACAGGCGGGGAACCCCTGCTGCGGGAAGATATTTACGAGCTGGCCGCTTAC
GGAGACGGACTGGGATTGCGAATGGTGCTGGCGACGAACGGCACCCTTGTCACGGAAGGGATCGCCCGGCGGATGCGGGA
GTCGGGAATCCAACGGGTCAGCATCAGTCTGGACGGGCCGGATGCGGAAAGTCACGATGCCTTTCGGCAGATGTCCGGGG
CTTTTGCCGGTGCAATGACGGGGATTGCCGCCTTAAAAAAAGCGGGAATGGAATTTCAGGTCAATACGACGATCACCAGC
ACCAACAGGCATCAGCTTTCCGCCATGATGGATCTGGCCGTTCGCCTTGGGGCCGCGGCCCATCATATCTTCCTGCTGGT
GCCCACCGGACGGGGACGGGATCTGGCGAATCAGGCCATCAGCGCGCAGGATTACGAAGACACTCTGCAGGCCTTTCATT
CGGCGAGCCTGACCTGTCCGATTCCCCTGAAGGCCACCTGTGCGCCGCACTATTACCGCATCCTGCATCAGAAGGGCTCC
GCCGGGAAAAGACCTTCCACTGAAGGGCACGGGACTCTGCATGCCGTGACCCGGGGATGTCTGGGAGGAATTTCCTTCTG
CTTTATTTCTCATACCGGTCAGGTGCAGCCCTGTGGCTATCTGGAACTGAATTGCGGGAATATTCGAAAAGCCGGTTTTG
AAATGATCTGGAAGAATGCCGCCGTTTTTCAGAAATTGAGGAATCCAGAGGCATTCTCGGGGAAATGCGGCCACTGTGAA
TTCTTCCGGGTATGCGGGGGCTGCCGCGCCCGAGCGTATGAGCTGACGGGCGATTATCTGGCGGAAGAGCCGTTCTGCAT
TTACGAACCGGCGAGGAAGGCGGCCGCCTTGCCATCCCGAGGGGAAACGGAACCGGGGAGTCCCGAATAG

Upstream 100 bases:

>100_bases
AAGAAAAAGCCATGATGGAATCTTTGACGGCCATCCGCCGTGCCGGTGCGGACATCATTCTGACCTATTTCGCTCGGGAA
GCGGCGAAAGTTCTCCAGCG

Downstream 100 bases:

>100_bases
GCCGGACAGGTAAACATGGACGGGATCGATAAAAGAATTCTGAATATGCTTCAGACGGAATTTCCCGTGACGCCGGAGCC
GTTCAGGGCGGTTGCGGAAA

Product: radical SAM protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNSSPLPSTLRMVAWEVTRSCNLSCIHCRASAEKGPYAGELSTEESKNLLEEIAAFSRPVVILTGGEPLLREDIYELAAY
GDGLGLRMVLATNGTLVTEGIARRMRESGIQRVSISLDGPDAESHDAFRQMSGAFAGAMTGIAALKKAGMEFQVNTTITS
TNRHQLSAMMDLAVRLGAAAHHIFLLVPTGRGRDLANQAISAQDYEDTLQAFHSASLTCPIPLKATCAPHYYRILHQKGS
AGKRPSTEGHGTLHAVTRGCLGGISFCFISHTGQVQPCGYLELNCGNIRKAGFEMIWKNAAVFQKLRNPEAFSGKCGHCE
FFRVCGGCRARAYELTGDYLAEEPFCIYEPARKAAALPSRGETEPGSPE

Sequences:

>Translated_369_residues
MNSSPLPSTLRMVAWEVTRSCNLSCIHCRASAEKGPYAGELSTEESKNLLEEIAAFSRPVVILTGGEPLLREDIYELAAY
GDGLGLRMVLATNGTLVTEGIARRMRESGIQRVSISLDGPDAESHDAFRQMSGAFAGAMTGIAALKKAGMEFQVNTTITS
TNRHQLSAMMDLAVRLGAAAHHIFLLVPTGRGRDLANQAISAQDYEDTLQAFHSASLTCPIPLKATCAPHYYRILHQKGS
AGKRPSTEGHGTLHAVTRGCLGGISFCFISHTGQVQPCGYLELNCGNIRKAGFEMIWKNAAVFQKLRNPEAFSGKCGHCE
FFRVCGGCRARAYELTGDYLAEEPFCIYEPARKAAALPSRGETEPGSPE
>Mature_369_residues
MNSSPLPSTLRMVAWEVTRSCNLSCIHCRASAEKGPYAGELSTEESKNLLEEIAAFSRPVVILTGGEPLLREDIYELAAY
GDGLGLRMVLATNGTLVTEGIARRMRESGIQRVSISLDGPDAESHDAFRQMSGAFAGAMTGIAALKKAGMEFQVNTTITS
TNRHQLSAMMDLAVRLGAAAHHIFLLVPTGRGRDLANQAISAQDYEDTLQAFHSASLTCPIPLKATCAPHYYRILHQKGS
AGKRPSTEGHGTLHAVTRGCLGGISFCFISHTGQVQPCGYLELNCGNIRKAGFEMIWKNAAVFQKLRNPEAFSGKCGHCE
FFRVCGGCRARAYELTGDYLAEEPFCIYEPARKAAALPSRGETEPGSPE

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Escherichia coli, GI1786999, Length=228, Percent_Identity=28.5087719298246, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39848; Mature: 39848

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSPLPSTLRMVAWEVTRSCNLSCIHCRASAEKGPYAGELSTEESKNLLEEIAAFSRPV
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
VILTGGEPLLREDIYELAAYGDGLGLRMVLATNGTLVTEGIARRMRESGIQRVSISLDGP
EEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCEEEEEEECCC
DAESHDAFRQMSGAFAGAMTGIAALKKAGMEFQVNTTITSTNRHQLSAMMDLAVRLGAAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCE
HHIFLLVPTGRGRDLANQAISAQDYEDTLQAFHSASLTCPIPLKATCAPHYYRILHQKGS
EEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCC
AGKRPSTEGHGTLHAVTRGCLGGISFCFISHTGQVQPCGYLELNCGNIRKAGFEMIWKNA
CCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHH
AVFQKLRNPEAFSGKCGHCEFFRVCGGCRARAYELTGDYLAEEPFCIYEPARKAAALPSR
HHHHHHCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCHHCCCCEEEECCHHHHHCCCCC
GETEPGSPE
CCCCCCCCC
>Mature Secondary Structure
MNSSPLPSTLRMVAWEVTRSCNLSCIHCRASAEKGPYAGELSTEESKNLLEEIAAFSRPV
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
VILTGGEPLLREDIYELAAYGDGLGLRMVLATNGTLVTEGIARRMRESGIQRVSISLDGP
EEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCEEEEEEECCC
DAESHDAFRQMSGAFAGAMTGIAALKKAGMEFQVNTTITSTNRHQLSAMMDLAVRLGAAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCE
HHIFLLVPTGRGRDLANQAISAQDYEDTLQAFHSASLTCPIPLKATCAPHYYRILHQKGS
EEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCC
AGKRPSTEGHGTLHAVTRGCLGGISFCFISHTGQVQPCGYLELNCGNIRKAGFEMIWKNA
CCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHH
AVFQKLRNPEAFSGKCGHCEFFRVCGGCRARAYELTGDYLAEEPFCIYEPARKAAALPSR
HHHHHHCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCHHCCCCEEEECCHHHHHCCCCC
GETEPGSPE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA