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The map label for this gene is guaA

Identifier: 85375169

GI number: 85375169

Start: 2356604

End: 2358178

Strand: Reverse

Name: guaA

Synonym: ELI_11710

Alternate gene names: 85375169

Gene position: 2358178-2356604 (Counterclockwise)

Preceding gene: 85375171

Following gene: 85375167

Centisome position: 77.26

GC content: 64.95

Gene sequence:

>1575_bases
ATGGACCAAGCGCATCTCCCCGATTCCATCCTCATCGTCGATTTCGGCAGCCAGGTGACCCAGCTGATCGCGCGCCGCGT
GCGCGAAGCCGGGGTCTATTCCGAAATCGCGCCCTTCAGCATGGCGGAAGAGGCCTTCCAACGGATGAAGCCCAAGGGCA
TCATCTTATCCGGCTCGCCCGCCGGGGTGCCGGACGAAGGCTCCCCGCGCGCACCGCAAATACTGTTCGAAGCCGGCGTG
CCGATCCTCGGCATCTGTTATGGCCAGCAGGTGATGACGCACCAGCTCGGCGGCGAAGTGCGGCCCGGACACGAGACCGG
CGACGGCGGCGAATTCGGGCGCGCCTACCTGACCGTAACCAAGGACTGCGCGCTGTTCGACGGCATGTGGCAGGTCGGCG
AGCGGCACCAGGTCTGGATGAGCCATGGCGACAAGGTCACGCAATTCGCCGACGGCTTCGAGATCGTCGCCACCAGCGAT
GGCGCGCCTTTCGCGGTTATCGCGGACGAAGCCCGCAAATTCTACGGCACCCAGTTCCACCCCGAAGTCGTCCATACGCC
CGACGGAGCCAAGCTGCTGGCCAATTTCGTCCGCCATGTCTGCGGGCTGGCGGGCGACTGGACCATGGCCGAATTCCGCA
AGACCAAGATCGAGGAAATCCGCGCGCAGGTGGGTGACGGCAAGGTCATCTGCGGCCTTAGCGGCGGTGTCGACAGCGCG
GTTGCGGCGGTGCTCATTCACGAAGCGATCGGCGAGCAACTGACCTGCGTCTTCGTCGACCACGGGCTGATGCGGCAGGG
CGAGGCCGAGCAGGTCGTGAGCCTGTTCCGCGGGCACTACAATATCCCGCTCGTCCATGTGGATGCGGAGGATATGTTCC
TCGGCGGGCTCGCGGGCGTCACCGACCCGGAGAAAAAGCGCAAGTTTATCGGCGGGGCCTTCATCGACCTGTTCGAGGAA
GAGGCGCGCAAGATCGGCGGGGCGGATTTCCTCGCCCAGGGCACGCTCTATCCCGACGTGATCGAATCGGTATCGTTCAC
CGGCGGGCCCAGCGTCACGATCAAGAGCCACCACAATGTCGGCGGACTGCCCGAGCGGATGAACATGCAATTGGTCGAGC
CTTTGCGCGAATTGTTCAAGGACGAGGTCCGCGCGCTGGGCCGCGAGCTCGGCCTACCCGATGTCTTCGTCGGCCGCCAC
CCCTTCCCCGGCCCCGGCCTCGCCATCCGCATCCCCGGCGAAGTCACCAAGGAACGCTGCGATATCCTGCGCAAGGCCGA
TGCGATCTATCTCGAGGAGATTCGCGCGGCCGGTCTCTACGATGCGATCTGGCAGGCCTTCGCCGTATTGCTCCCCGTCC
GCACTGTCGGCGTAATGGGCGATGCGCGCACCTATGACTCGGTCTGCGGCCTGCGCGCCGTGACCAGCACCGACGGCATG
ACGGCGGATGTCTATCCCTTCGACGCGACCTTCCTGACGCAGGTGGCGACGCGGATCGTCAACGAGGTGCAGGGCATCAA
CCGGGTGGTCTATGACTACACGTCGAAGCCGCCGGGGACGATCGAGTGGGAATAG

Upstream 100 bases:

>100_bases
GTCGAAGATCGCCTGTAAAAGCGCGCTATGGCGCATGCGGTGGGACAATCGACCACCGCTGCTGGACAGGCGGGCGGAGC
GCACTTAACCGCTCTTGCCC

Downstream 100 bases:

>100_bases
CGCTGACTCGGGGCCGTCCGGGAGACGGGCTCGGCAGCGCTATTCGCGAGCGCAAGCGAAGCGCACTGACACGGATCGAT
CCGGGGGATCGATCCGGCCA

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MDQAHLPDSILIVDFGSQVTQLIARRVREAGVYSEIAPFSMAEEAFQRMKPKGIILSGSPAGVPDEGSPRAPQILFEAGV
PILGICYGQQVMTHQLGGEVRPGHETGDGGEFGRAYLTVTKDCALFDGMWQVGERHQVWMSHGDKVTQFADGFEIVATSD
GAPFAVIADEARKFYGTQFHPEVVHTPDGAKLLANFVRHVCGLAGDWTMAEFRKTKIEEIRAQVGDGKVICGLSGGVDSA
VAAVLIHEAIGEQLTCVFVDHGLMRQGEAEQVVSLFRGHYNIPLVHVDAEDMFLGGLAGVTDPEKKRKFIGGAFIDLFEE
EARKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMQLVEPLRELFKDEVRALGRELGLPDVFVGRH
PFPGPGLAIRIPGEVTKERCDILRKADAIYLEEIRAAGLYDAIWQAFAVLLPVRTVGVMGDARTYDSVCGLRAVTSTDGM
TADVYPFDATFLTQVATRIVNEVQGINRVVYDYTSKPPGTIEWE

Sequences:

>Translated_524_residues
MDQAHLPDSILIVDFGSQVTQLIARRVREAGVYSEIAPFSMAEEAFQRMKPKGIILSGSPAGVPDEGSPRAPQILFEAGV
PILGICYGQQVMTHQLGGEVRPGHETGDGGEFGRAYLTVTKDCALFDGMWQVGERHQVWMSHGDKVTQFADGFEIVATSD
GAPFAVIADEARKFYGTQFHPEVVHTPDGAKLLANFVRHVCGLAGDWTMAEFRKTKIEEIRAQVGDGKVICGLSGGVDSA
VAAVLIHEAIGEQLTCVFVDHGLMRQGEAEQVVSLFRGHYNIPLVHVDAEDMFLGGLAGVTDPEKKRKFIGGAFIDLFEE
EARKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMQLVEPLRELFKDEVRALGRELGLPDVFVGRH
PFPGPGLAIRIPGEVTKERCDILRKADAIYLEEIRAAGLYDAIWQAFAVLLPVRTVGVMGDARTYDSVCGLRAVTSTDGM
TADVYPFDATFLTQVATRIVNEVQGINRVVYDYTSKPPGTIEWE
>Mature_524_residues
MDQAHLPDSILIVDFGSQVTQLIARRVREAGVYSEIAPFSMAEEAFQRMKPKGIILSGSPAGVPDEGSPRAPQILFEAGV
PILGICYGQQVMTHQLGGEVRPGHETGDGGEFGRAYLTVTKDCALFDGMWQVGERHQVWMSHGDKVTQFADGFEIVATSD
GAPFAVIADEARKFYGTQFHPEVVHTPDGAKLLANFVRHVCGLAGDWTMAEFRKTKIEEIRAQVGDGKVICGLSGGVDSA
VAAVLIHEAIGEQLTCVFVDHGLMRQGEAEQVVSLFRGHYNIPLVHVDAEDMFLGGLAGVTDPEKKRKFIGGAFIDLFEE
EARKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMQLVEPLRELFKDEVRALGRELGLPDVFVGRH
PFPGPGLAIRIPGEVTKERCDILRKADAIYLEEIRAAGLYDAIWQAFAVLLPVRTVGVMGDARTYDSVCGLRAVTSTDGM
TADVYPFDATFLTQVATRIVNEVQGINRVVYDYTSKPPGTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4504035, Length=568, Percent_Identity=35.9154929577465, Blast_Score=297, Evalue=2e-80,
Organism=Escherichia coli, GI1788854, Length=520, Percent_Identity=56.1538461538462, Blast_Score=583, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71992710, Length=574, Percent_Identity=36.0627177700348, Blast_Score=285, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI71992717, Length=574, Percent_Identity=36.0627177700348, Blast_Score=284, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI133901714, Length=574, Percent_Identity=36.0627177700348, Blast_Score=284, Evalue=9e-77,
Organism=Saccharomyces cerevisiae, GI6323873, Length=528, Percent_Identity=53.030303030303, Blast_Score=530, Evalue=1e-151,
Organism=Drosophila melanogaster, GI281365319, Length=553, Percent_Identity=33.996383363472, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 57016; Mature: 57016

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQAHLPDSILIVDFGSQVTQLIARRVREAGVYSEIAPFSMAEEAFQRMKPKGIILSGSP
CCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCEEEECCC
AGVPDEGSPRAPQILFEAGVPILGICYGQQVMTHQLGGEVRPGHETGDGGEFGRAYLTVT
CCCCCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEE
KDCALFDGMWQVGERHQVWMSHGDKVTQFADGFEIVATSDGAPFAVIADEARKFYGTQFH
CHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCCEEEEEECCCCCEEEEEHHHHHHHCCCCC
PEVVHTPDGAKLLANFVRHVCGLAGDWTMAEFRKTKIEEIRAQVGDGKVICGLSGGVDSA
CCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
VAAVLIHEAIGEQLTCVFVDHGLMRQGEAEQVVSLFRGHYNIPLVHVDAEDMFLGGLAGV
HHHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHHHHCCCCCCEEEEECHHHHHHCCCCCC
TDPEKKRKFIGGAFIDLFEEEARKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHCCCCCCCEEEEECCCCC
GGLPERMNMQLVEPLRELFKDEVRALGRELGLPDVFVGRHPFPGPGLAIRIPGEVTKERC
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEECCCHHHHHHH
DILRKADAIYLEEIRAAGLYDAIWQAFAVLLPVRTVGVMGDARTYDSVCGLRAVTSTDGM
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCC
TADVYPFDATFLTQVATRIVNEVQGINRVVYDYTSKPPGTIEWE
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDQAHLPDSILIVDFGSQVTQLIARRVREAGVYSEIAPFSMAEEAFQRMKPKGIILSGSP
CCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCEEEECCC
AGVPDEGSPRAPQILFEAGVPILGICYGQQVMTHQLGGEVRPGHETGDGGEFGRAYLTVT
CCCCCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEE
KDCALFDGMWQVGERHQVWMSHGDKVTQFADGFEIVATSDGAPFAVIADEARKFYGTQFH
CHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCCEEEEEECCCCCEEEEEHHHHHHHCCCCC
PEVVHTPDGAKLLANFVRHVCGLAGDWTMAEFRKTKIEEIRAQVGDGKVICGLSGGVDSA
CCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
VAAVLIHEAIGEQLTCVFVDHGLMRQGEAEQVVSLFRGHYNIPLVHVDAEDMFLGGLAGV
HHHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHHHHCCCCCCEEEEECHHHHHHCCCCCC
TDPEKKRKFIGGAFIDLFEEEARKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHCCCCCCCEEEEECCCCC
GGLPERMNMQLVEPLRELFKDEVRALGRELGLPDVFVGRHPFPGPGLAIRIPGEVTKERC
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEECCCHHHHHHH
DILRKADAIYLEEIRAAGLYDAIWQAFAVLLPVRTVGVMGDARTYDSVCGLRAVTSTDGM
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCC
TADVYPFDATFLTQVATRIVNEVQGINRVVYDYTSKPPGTIEWE
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA