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The map label for this gene is lcfB [H]

Identifier: 85375171

GI number: 85375171

Start: 2358933

End: 2360471

Strand: Reverse

Name: lcfB [H]

Synonym: ELI_11720

Alternate gene names: 85375171

Gene position: 2360471-2358933 (Counterclockwise)

Preceding gene: 85375177

Following gene: 85375169

Centisome position: 77.33

GC content: 64.78

Gene sequence:

>1539_bases
ATGCTTGAGAGACTGCAGCAGCCCTATGGCTCGTTTCCCGCAATCCTGTCGGCCTGGGCCGAGCGGCAGCCGGACAAGCC
TGCGTTGCGCGACGATGCGGGCGAAATGAGCTGGGCCGAGCTCGATGGTCGCGTCGAGCGGCTGGCGGCGAAACTGGTCG
AGACCGGGCTCGAACGGGGGCAATCGGTCGCGATCCTCGGGACGAGTTGTATCGCCTATGCCCTGGTGTTTCTCGCTGCG
GTGCGCGCAGGGGGGGTGGCCGCGCCGCTGACGACCAGCGCCAGCCGCGAACAGCTTGAAGGCATGGCGAAAGATTCAGG
TGCGCGGCACCTGTTCATCGATGACGGCAAGAACGCCGAGCTGGGCGAGGATTTCCTGCCCGGCTTCGATCGCATCGCGC
TGGAAGAAATCGACGCGTGGATGGCCCCGGAAGGCACGAAGGCTCCCGCGTTCGATCCCGATCCCGCAGACCATTTCAAC
ATCATCTATTCCAGCGGCACTACTGGCGTGCCCAAGGGCATCGTCCATTCGCACCAGATGCGCTGGCGGCAGTTCGCGGC
GACCGCTTCCAGCTATATCGATGCCGGGCTCGAGGTACGCTCGCTCGCCTCGACGCCGCTTTATTCGAACACCACCATGG
TTGCGTTTCTTGCTCCGCTGCTGGCAGGCGGCACGGTGCGGATCATGGGCAAATTCAACACCGTGCGCTGGCTCGAGCAT
GCGCAGGCGGACAGGACTATTATCACCATGCTGGTGCCGGTCCAGTACCAGCGGCTGATGGCCGAGCCGAGTTTCGACCA
ATACGACCTGTCGTCGCTGGCGCTCAAATACTGCACCTCCGCGCCGTTCTCGGCGGAACTCAAGGCGGAGGTGCTGCAAC
GCATGCCGGGCGGCCTGATCGAGATCTACTCGATGACCGAAGGCGGTGTCGTGTGCCTGCTCGCCTGCCATGAATTTCCG
GACAAATTGCACACCGTCGGGCGGCCCGCACCGGGCAGCGAGCTGAAAGTGCTGGACGACGAGGATCGCCTTGTCCCGCC
GGGCGAGCCGGGCAACCTCATCGGCCGCTCGCAGACCATGATGGCGGGCTACAAGAACCAGCCCGGCAAAACCCGCGAAG
GCTACTGGACCGATCCGGACACCGGCGAGGTCTGGCAACGGATGGGCGATATCGGGCGGGTCGACGAAGACGGCTTCGTC
GAACTTGTCGGGCGGGCCAAGGACATGATCATCTCGGGCGGGTTCAATATCTATCCGAGCGATCTGGAAGCCGAGCTGGA
GAAGGACCCGCGCGTCGCCGAAGCGGCGGTGATCGGTGTCGCGAGCGAGCAATGGGGCGAAACCCCGCTCGGCTTCGTGC
GGCTGATGCCGGCGGTGGCGGAGGACGAGGCCGAGGACATCCTCGGTACGGTCAATGCGCGGCTGGGCAAGACCCAGCGG
CTCTCCGCGCTGCACGTGATCGAGGAAATGCCGCGCAGCCATATCGGCAAACTGCTGAAGACCGAACTGCGCGACCTGGC
CGAAACGCTGGCGGGCTGA

Upstream 100 bases:

>100_bases
AGCAAAGCGCGGGTGCATGGCGGTCTCCCCTCTGGCCAAAATCTCGGCATGACCTTAGGCGGTTGATATCGCGGTGCAAG
GTGAGAGAGGACCCGAGTCT

Downstream 100 bases:

>100_bases
TCGTCCGGAAAGAGAGATGAGGTGCGGCTTGCCGCCTGGGGGAGGAGGGGGAAGCGGTCGGCAAGCCGCACCTCGGGCGA
CCCGGACGGGCCGGGTGGTT

Product: putative 4-coumarate:CoA ligase 2

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLERLQQPYGSFPAILSAWAERQPDKPALRDDAGEMSWAELDGRVERLAAKLVETGLERGQSVAILGTSCIAYALVFLAA
VRAGGVAAPLTTSASREQLEGMAKDSGARHLFIDDGKNAELGEDFLPGFDRIALEEIDAWMAPEGTKAPAFDPDPADHFN
IIYSSGTTGVPKGIVHSHQMRWRQFAATASSYIDAGLEVRSLASTPLYSNTTMVAFLAPLLAGGTVRIMGKFNTVRWLEH
AQADRTIITMLVPVQYQRLMAEPSFDQYDLSSLALKYCTSAPFSAELKAEVLQRMPGGLIEIYSMTEGGVVCLLACHEFP
DKLHTVGRPAPGSELKVLDDEDRLVPPGEPGNLIGRSQTMMAGYKNQPGKTREGYWTDPDTGEVWQRMGDIGRVDEDGFV
ELVGRAKDMIISGGFNIYPSDLEAELEKDPRVAEAAVIGVASEQWGETPLGFVRLMPAVAEDEAEDILGTVNARLGKTQR
LSALHVIEEMPRSHIGKLLKTELRDLAETLAG

Sequences:

>Translated_512_residues
MLERLQQPYGSFPAILSAWAERQPDKPALRDDAGEMSWAELDGRVERLAAKLVETGLERGQSVAILGTSCIAYALVFLAA
VRAGGVAAPLTTSASREQLEGMAKDSGARHLFIDDGKNAELGEDFLPGFDRIALEEIDAWMAPEGTKAPAFDPDPADHFN
IIYSSGTTGVPKGIVHSHQMRWRQFAATASSYIDAGLEVRSLASTPLYSNTTMVAFLAPLLAGGTVRIMGKFNTVRWLEH
AQADRTIITMLVPVQYQRLMAEPSFDQYDLSSLALKYCTSAPFSAELKAEVLQRMPGGLIEIYSMTEGGVVCLLACHEFP
DKLHTVGRPAPGSELKVLDDEDRLVPPGEPGNLIGRSQTMMAGYKNQPGKTREGYWTDPDTGEVWQRMGDIGRVDEDGFV
ELVGRAKDMIISGGFNIYPSDLEAELEKDPRVAEAAVIGVASEQWGETPLGFVRLMPAVAEDEAEDILGTVNARLGKTQR
LSALHVIEEMPRSHIGKLLKTELRDLAETLAG
>Mature_512_residues
MLERLQQPYGSFPAILSAWAERQPDKPALRDDAGEMSWAELDGRVERLAAKLVETGLERGQSVAILGTSCIAYALVFLAA
VRAGGVAAPLTTSASREQLEGMAKDSGARHLFIDDGKNAELGEDFLPGFDRIALEEIDAWMAPEGTKAPAFDPDPADHFN
IIYSSGTTGVPKGIVHSHQMRWRQFAATASSYIDAGLEVRSLASTPLYSNTTMVAFLAPLLAGGTVRIMGKFNTVRWLEH
AQADRTIITMLVPVQYQRLMAEPSFDQYDLSSLALKYCTSAPFSAELKAEVLQRMPGGLIEIYSMTEGGVVCLLACHEFP
DKLHTVGRPAPGSELKVLDDEDRLVPPGEPGNLIGRSQTMMAGYKNQPGKTREGYWTDPDTGEVWQRMGDIGRVDEDGFV
ELVGRAKDMIISGGFNIYPSDLEAELEKDPRVAEAAVIGVASEQWGETPLGFVRLMPAVAEDEAEDILGTVNARLGKTQR
LSALHVIEEMPRSHIGKLLKTELRDLAETLAG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=551, Percent_Identity=25.9528130671506, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI42544132, Length=533, Percent_Identity=23.2645403377111, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI157311624, Length=493, Percent_Identity=25.7606490872211, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI157311622, Length=493, Percent_Identity=25.7606490872211, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI187761345, Length=541, Percent_Identity=26.6173752310536, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI187761343, Length=541, Percent_Identity=26.6173752310536, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI115511026, Length=544, Percent_Identity=23.8970588235294, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI58082049, Length=492, Percent_Identity=24.5934959349593, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI38505220, Length=537, Percent_Identity=22.5325884543762, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI122937307, Length=538, Percent_Identity=24.1635687732342, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1788107, Length=548, Percent_Identity=27.3722627737226, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI145693145, Length=516, Percent_Identity=25, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786810, Length=502, Percent_Identity=24.9003984063745, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786801, Length=459, Percent_Identity=25.4901960784314, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI221142682, Length=496, Percent_Identity=23.5887096774194, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788595, Length=464, Percent_Identity=24.3534482758621, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786529, Length=564, Percent_Identity=21.2765957446809, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32563687, Length=516, Percent_Identity=24.6124031007752, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17558820, Length=494, Percent_Identity=26.5182186234818, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71996755, Length=506, Percent_Identity=25.8893280632411, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=561, Percent_Identity=26.0249554367201, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=548, Percent_Identity=24.4525547445255, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=548, Percent_Identity=24.4525547445255, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=548, Percent_Identity=24.4525547445255, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=505, Percent_Identity=26.1386138613861, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=516, Percent_Identity=24.8062015503876, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560140, Length=531, Percent_Identity=25.4237288135593, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=519, Percent_Identity=27.5529865125241, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=25.631067961165, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=355, Percent_Identity=25.9154929577465, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25141359, Length=452, Percent_Identity=22.5663716814159, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115534440, Length=559, Percent_Identity=22.7191413237925, Blast_Score=86, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=490, Percent_Identity=22.4489795918367, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21355181, Length=510, Percent_Identity=27.2549019607843, Blast_Score=147, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24581924, Length=368, Percent_Identity=27.9891304347826, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648253, Length=482, Percent_Identity=24.6887966804979, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648255, Length=482, Percent_Identity=24.6887966804979, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI18859661, Length=358, Percent_Identity=28.7709497206704, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356441, Length=347, Percent_Identity=27.9538904899135, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356947, Length=371, Percent_Identity=26.4150943396226, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI281365686, Length=357, Percent_Identity=27.4509803921569, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21358303, Length=466, Percent_Identity=23.3905579399142, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19922652, Length=499, Percent_Identity=23.8476953907816, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20130357, Length=508, Percent_Identity=24.0157480314961, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648260, Length=516, Percent_Identity=22.6744186046512, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653035, Length=351, Percent_Identity=23.3618233618234, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648257, Length=294, Percent_Identity=26.530612244898, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648676, Length=344, Percent_Identity=26.7441860465116, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24656500, Length=499, Percent_Identity=21.8436873747495, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 55732; Mature: 55732

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERLQQPYGSFPAILSAWAERQPDKPALRDDAGEMSWAELDGRVERLAAKLVETGLERG
CCHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
QSVAILGTSCIAYALVFLAAVRAGGVAAPLTTSASREQLEGMAKDSGARHLFIDDGKNAE
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCC
LGEDFLPGFDRIALEEIDAWMAPEGTKAPAFDPDPADHFNIIYSSGTTGVPKGIVHSHQM
CCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
RWRQFAATASSYIDAGLEVRSLASTPLYSNTTMVAFLAPLLAGGTVRIMGKFNTVRWLEH
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHH
AQADRTIITMLVPVQYQRLMAEPSFDQYDLSSLALKYCTSAPFSAELKAEVLQRMPGGLI
HCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEE
EIYSMTEGGVVCLLACHEFPDKLHTVGRPAPGSELKVLDDEDRLVPPGEPGNLIGRSQTM
EEEEECCCCEEEEEEHHHCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHH
MAGYKNQPGKTREGYWTDPDTGEVWQRMGDIGRVDEDGFVELVGRAKDMIISGGFNIYPS
HCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCC
DLEAELEKDPRVAEAAVIGVASEQWGETPLGFVRLMPAVAEDEAEDILGTVNARLGKTQR
CHHHHHHCCCCHHHHHHEECCHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
LSALHVIEEMPRSHIGKLLKTELRDLAETLAG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLERLQQPYGSFPAILSAWAERQPDKPALRDDAGEMSWAELDGRVERLAAKLVETGLERG
CCHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
QSVAILGTSCIAYALVFLAAVRAGGVAAPLTTSASREQLEGMAKDSGARHLFIDDGKNAE
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCC
LGEDFLPGFDRIALEEIDAWMAPEGTKAPAFDPDPADHFNIIYSSGTTGVPKGIVHSHQM
CCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
RWRQFAATASSYIDAGLEVRSLASTPLYSNTTMVAFLAPLLAGGTVRIMGKFNTVRWLEH
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHH
AQADRTIITMLVPVQYQRLMAEPSFDQYDLSSLALKYCTSAPFSAELKAEVLQRMPGGLI
HCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEE
EIYSMTEGGVVCLLACHEFPDKLHTVGRPAPGSELKVLDDEDRLVPPGEPGNLIGRSQTM
EEEEECCCCEEEEEEHHHCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHH
MAGYKNQPGKTREGYWTDPDTGEVWQRMGDIGRVDEDGFVELVGRAKDMIISGGFNIYPS
HCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCC
DLEAELEKDPRVAEAAVIGVASEQWGETPLGFVRLMPAVAEDEAEDILGTVNARLGKTQR
CHHHHHHCCCCHHHHHHEECCHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
LSALHVIEEMPRSHIGKLLKTELRDLAETLAG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]