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The map label for this gene is iorA [H]

Identifier: 85375073

GI number: 85375073

Start: 2271027

End: 2271512

Strand: Reverse

Name: iorA [H]

Synonym: ELI_11230

Alternate gene names: 85375073

Gene position: 2271512-2271027 (Counterclockwise)

Preceding gene: 85375074

Following gene: 85375072

Centisome position: 74.42

GC content: 62.76

Gene sequence:

>486_bases
ATGATCGAATTCACGCTCAATGGAAATGCCGTTTCGGTCGATGCCGATCCGGCCATGCCGATCCTGTGGGTCGTCCGCGA
GAAGCTCGGAATGAACGGCACGAAGTTCGGCTGCGGGATCGCGCAGTGCGGGGCGTGCACCGTACATCTGGACGGCAAAC
CGATCCGCAGTTGTTCGACGCCGGTTTCGGAAGCCGAAGGTCGCTTGGTCACGACGATCGAAGGCATTGCCGGACCTGAA
GGCGAACTGACGAAGGTCCAGCAAGCGTGGATCAGCGAACAGGTACCGCAATGCGGCTATTGCCAGTCGGGCCAGATCAT
GGCGGCGACGGCGCTGCTGCGCGATACGCCGCAACCGAGCGACGCACAGATCGATAGTGCGATGGCGGGCAATATCTGCC
GCTGCGGCACTTACACCCGCATTCGCCGCGCCATCAAAGTCGCCGCCGGCCTGCAAGAGCCCCGCAACGAGGAGGCGGAG
GCATGA

Upstream 100 bases:

>100_bases
CCCCCGCTCTTGCGGGGATCGCCCATTGCCTTGACGCGCCCGCTCCGGCACACTTGCGTTGCAAGTCGCAACGCAAACCG
CCGCCGGAGAAAGAGCCGCG

Downstream 100 bases:

>100_bases
GCGAGCAAGCCGATACCAAGCCGGCGCGCAGCCGTACGGCCAAATGGACTAGGCGCGGGGTCATGGGTGCAGGCGTGCTT
GCCGGCGGCGCGTTGCTGAT

Product: isoquinoline 1-oxidoreductase, alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MIEFTLNGNAVSVDADPAMPILWVVREKLGMNGTKFGCGIAQCGACTVHLDGKPIRSCSTPVSEAEGRLVTTIEGIAGPE
GELTKVQQAWISEQVPQCGYCQSGQIMAATALLRDTPQPSDAQIDSAMAGNICRCGTYTRIRRAIKVAAGLQEPRNEEAE
A

Sequences:

>Translated_161_residues
MIEFTLNGNAVSVDADPAMPILWVVREKLGMNGTKFGCGIAQCGACTVHLDGKPIRSCSTPVSEAEGRLVTTIEGIAGPE
GELTKVQQAWISEQVPQCGYCQSGQIMAATALLRDTPQPSDAQIDSAMAGNICRCGTYTRIRRAIKVAAGLQEPRNEEAE
A
>Mature_161_residues
MIEFTLNGNAVSVDADPAMPILWVVREKLGMNGTKFGCGIAQCGACTVHLDGKPIRSCSTPVSEAEGRLVTTIEGIAGPE
GELTKVQQAWISEQVPQCGYCQSGQIMAATALLRDTPQPSDAQIDSAMAGNICRCGTYTRIRRAIKVAAGLQEPRNEEAE
A

Specific function: Specific towards N-containing N-heterocyclic substrates, including isoquinoline, isoquinolin-5-ol, phthalazine and quinazoline [H]

COG id: COG2080

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, small subunit CoxS/CutS homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 2Fe-2S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI91823271, Length=157, Percent_Identity=36.9426751592357, Blast_Score=85, Evalue=3e-17,
Organism=Homo sapiens, GI71773480, Length=154, Percent_Identity=33.7662337662338, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1786480, Length=164, Percent_Identity=37.1951219512195, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1789232, Length=147, Percent_Identity=40.8163265306122, Blast_Score=100, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17540638, Length=151, Percent_Identity=39.0728476821192, Blast_Score=106, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17539860, Length=155, Percent_Identity=33.5483870967742, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32566215, Length=155, Percent_Identity=32.9032258064516, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17737937, Length=157, Percent_Identity=38.8535031847134, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24647193, Length=167, Percent_Identity=32.3353293413174, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24647195, Length=162, Percent_Identity=29.0123456790123, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24647197, Length=162, Percent_Identity=29.0123456790123, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24647199, Length=155, Percent_Identity=29.0322580645161, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002888
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR001041 [H]

Pfam domain/function: PF00111 Fer2; PF01799 Fer2_2 [H]

EC number: =1.3.99.16 [H]

Molecular weight: Translated: 17082; Mature: 17082

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFTLNGNAVSVDADPAMPILWVVREKLGMNGTKFGCGIAQCGACTVHLDGKPIRSCST
CEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
PVSEAEGRLVTTIEGIAGPEGELTKVQQAWISEQVPQCGYCQSGQIMAATALLRDTPQPS
CCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCC
DAQIDSAMAGNICRCGTYTRIRRAIKVAAGLQEPRNEEAEA
CCHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIEFTLNGNAVSVDADPAMPILWVVREKLGMNGTKFGCGIAQCGACTVHLDGKPIRSCST
CEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
PVSEAEGRLVTTIEGIAGPEGELTKVQQAWISEQVPQCGYCQSGQIMAATALLRDTPQPS
CCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCC
DAQIDSAMAGNICRCGTYTRIRRAIKVAAGLQEPRNEEAEA
CCHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7782304; 8157655 [H]