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The map label for this gene is nylB [H]

Identifier: 85374233

GI number: 85374233

Start: 1457153

End: 1458382

Strand: Reverse

Name: nylB [H]

Synonym: ELI_07030

Alternate gene names: 85374233

Gene position: 1458382-1457153 (Counterclockwise)

Preceding gene: 85374234

Following gene: 85374229

Centisome position: 47.78

GC content: 63.66

Gene sequence:

>1230_bases
GTGAAGAAATGGATCCTGGGCGGACTGGCCGTCATCGCCGCTGCAACGGGCGTCGGCTATGCGACGCTCGACGATGACCA
GAGAGCGCTGCTGGCCAATCTCCCGACCGATCGCAACGTGCTGTTCTGGTCGGTCGAGCAGCGCGATGCCGCCTTCCGGG
CGATGGACGCATTGCCGGTGCTGGCCGAAACGCGCACCATCGAAGCGGGCGGCGACCCCTATCCGCTGCCGGAGGGCCTG
CCGCTGGAGATGGATTTCGATCTCGATGCCTATTTCGCCAGCCAGCGCACCTCCGCGCTGGTGATATTGCATGATGGCAA
GATCGTGCTCGAACGCTATGGCCTCGGCTTCGACAAAGACGGCAAGTGGACGAGCTTTTCCGTCGCCAAGAGCCTCACCT
CGACCATGGTCGGCGCGGCACTGCAGGATGGCGCGATCGAGAGCATCGACGATCCCGTCACCAAATATATCGTCGGGCTG
GAGGGCAGTCCTTACGAGGACGTCTCGATCAAGCAATTGCTTACGATGACCAGCGGGGTGCGGTGGAACGAGGATTACAC
CGATCCGCAGTCCGACGTCGCCCAATTCAACGATCATCGCGCCGAGAACGGTCTCGACGTGACGGTGAGTTACATGCGCC
AGCTCCCGCGCGAGGCGCCTGCGGGCGAGAAATGGGTCTATAAGACCGGCGAGACCAATCTCATCGGCGTGCTGGTGAGC
GAAGCCACGGGCAAGCCGCTGGCCGAATACCTGTCCGAGAAAATCTGGAAGCCCTTCGGTATGCAGCAGGACGCGACCTG
GTTGCTCGGTGCTACGGGACATGAAATCAGCGGGTGCTGCATCCAGGCGGCGACGCGCGACATGGCGCGCTTCGGACTAT
TCGTGCTGCAGGACGGCGTGGCAGGTGGCGAGCGTGTCGTGCCCAGAGGCTGGTTCGCAGAAGCGACGGTGAAATCAGCC
GATATCGGCGCGCCCGGCAAAGGCTACGGCTACCAATGGTGGACCTATGACGACGGCGCCTATGCCGCGCAGGGTATTTT
CGGGCAGGGGATCTTCATCGACCCGGCGCGCAAGCTCGTGATCGCTTCGAACTCCAACTGGCCGACCGCCACCGATGCCG
ATGGCGTCGGGGCCGAGCGAGAGCGGTTCTACCAGGCGGTACGCGATGCGGTGGATCGCCGCGCGGGTCCGATCATGGAA
GCACGGGCAGGGGCAGACGCGGCTGAGTAG

Upstream 100 bases:

>100_bases
TGTGGCGATACTACCTGCAATATTGCGAAGCCGGCTTTCGCTGTGGCAGCATCGATGTACACCAAGTGACACTGGTGAAA
GAGGATACGGGAGAGGACGC

Downstream 100 bases:

>100_bases
CGTCCGCGCCCCCTAAGCGAAGGGATCGTACCACTGGCCGGCCTTGCCGGTGTAACGACGCAGGATGTCGTCGTGGACAA
TCGGGCTGAGCAGGTCCTCG

Product: hypothetical protein

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKKWILGGLAVIAAATGVGYATLDDDQRALLANLPTDRNVLFWSVEQRDAAFRAMDALPVLAETRTIEAGGDPYPLPEGL
PLEMDFDLDAYFASQRTSALVILHDGKIVLERYGLGFDKDGKWTSFSVAKSLTSTMVGAALQDGAIESIDDPVTKYIVGL
EGSPYEDVSIKQLLTMTSGVRWNEDYTDPQSDVAQFNDHRAENGLDVTVSYMRQLPREAPAGEKWVYKTGETNLIGVLVS
EATGKPLAEYLSEKIWKPFGMQQDATWLLGATGHEISGCCIQAATRDMARFGLFVLQDGVAGGERVVPRGWFAEATVKSA
DIGAPGKGYGYQWWTYDDGAYAAQGIFGQGIFIDPARKLVIASNSNWPTATDADGVGAERERFYQAVRDAVDRRAGPIME
ARAGADAAE

Sequences:

>Translated_409_residues
MKKWILGGLAVIAAATGVGYATLDDDQRALLANLPTDRNVLFWSVEQRDAAFRAMDALPVLAETRTIEAGGDPYPLPEGL
PLEMDFDLDAYFASQRTSALVILHDGKIVLERYGLGFDKDGKWTSFSVAKSLTSTMVGAALQDGAIESIDDPVTKYIVGL
EGSPYEDVSIKQLLTMTSGVRWNEDYTDPQSDVAQFNDHRAENGLDVTVSYMRQLPREAPAGEKWVYKTGETNLIGVLVS
EATGKPLAEYLSEKIWKPFGMQQDATWLLGATGHEISGCCIQAATRDMARFGLFVLQDGVAGGERVVPRGWFAEATVKSA
DIGAPGKGYGYQWWTYDDGAYAAQGIFGQGIFIDPARKLVIASNSNWPTATDADGVGAERERFYQAVRDAVDRRAGPIME
ARAGADAAE
>Mature_409_residues
MKKWILGGLAVIAAATGVGYATLDDDQRALLANLPTDRNVLFWSVEQRDAAFRAMDALPVLAETRTIEAGGDPYPLPEGL
PLEMDFDLDAYFASQRTSALVILHDGKIVLERYGLGFDKDGKWTSFSVAKSLTSTMVGAALQDGAIESIDDPVTKYIVGL
EGSPYEDVSIKQLLTMTSGVRWNEDYTDPQSDVAQFNDHRAENGLDVTVSYMRQLPREAPAGEKWVYKTGETNLIGVLVS
EATGKPLAEYLSEKIWKPFGMQQDATWLLGATGHEISGCCIQAATRDMARFGLFVLQDGVAGGERVVPRGWFAEATVKSA
DIGAPGKGYGYQWWTYDDGAYAAQGIFGQGIFIDPARKLVIASNSNWPTATDADGVGAERERFYQAVRDAVDRRAGPIME
ARAGADAAE

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 44416; Mature: 44416

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWILGGLAVIAAATGVGYATLDDDQRALLANLPTDRNVLFWSVEQRDAAFRAMDALPV
CCCHHHHHHHHHHHHHCCCEEEECCCCHHHEECCCCCCCEEEEEECHHHHHHHHHHHCHH
LAETRTIEAGGDPYPLPEGLPLEMDFDLDAYFASQRTSALVILHDGKIVLERYGLGFDKD
HHCCEEEECCCCCCCCCCCCCEEECCCCHHHHHCCCCCEEEEEECCEEEEEECCCCCCCC
GKWTSFSVAKSLTSTMVGAALQDGAIESIDDPVTKYIVGLEGSPYEDVSIKQLLTMTSGV
CCEEHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHEEEECCCCCCCCCHHHHHHHHHCCC
RWNEDYTDPQSDVAQFNDHRAENGLDVTVSYMRQLPREAPAGEKWVYKTGETNLIGVLVS
CCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHCCCCCCCCCCEEEECCCCEEEEEEEE
EATGKPLAEYLSEKIWKPFGMQQDATWLLGATGHEISGCCIQAATRDMARFGLFVLQDGV
CCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCC
AGGERVVPRGWFAEATVKSADIGAPGKGYGYQWWTYDDGAYAAQGIFGQGIFIDPARKLV
CCCCEECCCCCCHHCEECCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCEEECCCCEEE
IASNSNWPTATDADGVGAERERFYQAVRDAVDRRAGPIMEARAGADAAE
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKKWILGGLAVIAAATGVGYATLDDDQRALLANLPTDRNVLFWSVEQRDAAFRAMDALPV
CCCHHHHHHHHHHHHHCCCEEEECCCCHHHEECCCCCCCEEEEEECHHHHHHHHHHHCHH
LAETRTIEAGGDPYPLPEGLPLEMDFDLDAYFASQRTSALVILHDGKIVLERYGLGFDKD
HHCCEEEECCCCCCCCCCCCCEEECCCCHHHHHCCCCCEEEEEECCEEEEEECCCCCCCC
GKWTSFSVAKSLTSTMVGAALQDGAIESIDDPVTKYIVGLEGSPYEDVSIKQLLTMTSGV
CCEEHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHEEEECCCCCCCCCHHHHHHHHHCCC
RWNEDYTDPQSDVAQFNDHRAENGLDVTVSYMRQLPREAPAGEKWVYKTGETNLIGVLVS
CCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHCCCCCCCCCCEEEECCCCEEEEEEEE
EATGKPLAEYLSEKIWKPFGMQQDATWLLGATGHEISGCCIQAATRDMARFGLFVLQDGV
CCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCC
AGGERVVPRGWFAEATVKSADIGAPGKGYGYQWWTYDDGAYAAQGIFGQGIFIDPARKLV
CCCCEECCCCCCHHCEECCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCEEECCCCEEE
IASNSNWPTATDADGVGAERERFYQAVRDAVDRRAGPIMEARAGADAAE
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]