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The map label for this gene is nuoM [H]

Identifier: 85374148

GI number: 85374148

Start: 1371478

End: 1373025

Strand: Reverse

Name: nuoM [H]

Synonym: ELI_06605

Alternate gene names: 85374148

Gene position: 1373025-1371478 (Counterclockwise)

Preceding gene: 85374149

Following gene: 85374147

Centisome position: 44.98

GC content: 62.98

Gene sequence:

>1548_bases
ATGGAAGGCTTCCCTATCCTTACTGCTCTCCTGGTAGTGCCGCTGGTCGCGGCGCTTGCCTGCTATTTCCTCGAAGCGTC
GGCAGCGCGCATGGTCGCGCTGGTCGCCACGATGATCAATCTCGCGCTGGGCATCGCGCTGTGGGCAGGTTTCGAAATCG
GCGGTCCGCAGTGGCAGTTCACCGAGCGGGCAGACCTGTTTGCGGGATTCGACTATGCCCTCGGCGTCGATGGCATTGCG
ATGCTGCTCATTATGCTCAGCGTCTTCCTGATGCCGATCTGTGTCCTCGCCAGCTGGGAAGCGATCCAGAAGCGCGTCGG
CGAATACATGGCCGCGCTGTTGCTGACAGAGATGCTCATGATCGGCGTCTTCGCCGCGCAGGACTTGTTCCTGTTCTACA
TCTTCTTCGAAGCCGGCCTGATCCCGATGTACCTGCTGATCGGCATCTGGGGCGGGGCGGACCGCATCTACGCAGCGTTC
AAGTTCTTCCTCTATACGCTGATCGGCTCGGTCCTGATGCTGGTCGCAATGTTCTGGATGGTGAACGAAGCGGGCACCTC
CGACATCCCGACGCTGATGCAATACGATTTCGCGCCGGAAGCGCAGACCTGGCTGTGGCTGGCCTTCTTCGCGAGCTTCG
CGGTGAAAGTGCCGATGTTCCCCTTCCACACCTGGCTGCCCGCCGCACACGTGCAGGCGCCGACTGCCGGTTCGGTGATC
CTCGCCGGCGTGCTGCTCAAGCTCGGCGGCTACGGCTTCATTCGCTTCAGTCTGCCGATGTTCCCCGAAGCCTCCGCGCA
GTTCACGTGGCTGGTCTTTGCCCTGTCGATGATCGCGGTGATCTACACCTCGCTCGTTGCGCTGGTGCAGGAGGACATGA
AGAAACTGATCGCCTATTCCTCGGTCGCGCACATGGCGATCGTGACGGCGGCACTCTTCGCCTTCAACGTCCAGGGCCTC
GAAGGCGCAATGATCGTGATGCTGAGCCACGGCCTGGTGTCGGGCGCGCTCTTCCTGTGCGTGGGGATCATCTACGACCG
GCTGCATACGCGCGAGATCGGGCGTTACGGCGGGCTTGCCATCAACATGCCCAAATATGCGCTGTTCTTCCTCCTCTTCA
CCATGGCCAGCATCGGCCTGCCGGGCACAAGCGGCTTCGTCGCGGAATTCCTCAGCCTTGCCGGCACCTACCAGGTCTCC
AGCTCAGTGACGCTGTTCCTTACCACGGGCATCATCCTCGGAGCTGGCTACATGCTCTATCTCTATCGCCGGGTCGTCTT
CGGCACGCAGGTCAACGAGGACGCCGCGGCGATGAAGGATCTCAACCTGCGCGAGTGGGCGATGCTGGGCCCTATTGCCG
CCGCCGTGCTGTGGATGGGCGTCTATCCCGAAAGCTTCCTCGCCCCGATGCGGCAGGACATCGCTGCGCTCGACGCGCGC
CTGTCGCGCGCCGCGCCGGAAGGCGATGCGAAACTGGCGGCGGGTGAGCCACGGGCGGAGGCCGCCAATGCCGTCGCTTA
CGAGGCCAAGGAAGGGGGAGCGCACTGA

Upstream 100 bases:

>100_bases
GTTCTGGCGCGGCCAGGCGCCTGCAGTCCGGTTATCTCTACAGCTACGCGCTGATCATGCTGCTGGGCCTGGTCGCCGCG
GTTACCTGGGTGCTCCTGTG

Downstream 100 bases:

>100_bases
TGGAACTCAATGCATCCCTCACGCTGATCCTCCCCGAGATCGTCATGGCGATCTCGGCCATGGCGCTGATCCTGATCACT
GCCTATGTCGGCGACAAGAC

Product: NADH-quinone oxidoreductase chain M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MEGFPILTALLVVPLVAALACYFLEASAARMVALVATMINLALGIALWAGFEIGGPQWQFTERADLFAGFDYALGVDGIA
MLLIMLSVFLMPICVLASWEAIQKRVGEYMAALLLTEMLMIGVFAAQDLFLFYIFFEAGLIPMYLLIGIWGGADRIYAAF
KFFLYTLIGSVLMLVAMFWMVNEAGTSDIPTLMQYDFAPEAQTWLWLAFFASFAVKVPMFPFHTWLPAAHVQAPTAGSVI
LAGVLLKLGGYGFIRFSLPMFPEASAQFTWLVFALSMIAVIYTSLVALVQEDMKKLIAYSSVAHMAIVTAALFAFNVQGL
EGAMIVMLSHGLVSGALFLCVGIIYDRLHTREIGRYGGLAINMPKYALFFLLFTMASIGLPGTSGFVAEFLSLAGTYQVS
SSVTLFLTTGIILGAGYMLYLYRRVVFGTQVNEDAAAMKDLNLREWAMLGPIAAAVLWMGVYPESFLAPMRQDIAALDAR
LSRAAPEGDAKLAAGEPRAEAANAVAYEAKEGGAH

Sequences:

>Translated_515_residues
MEGFPILTALLVVPLVAALACYFLEASAARMVALVATMINLALGIALWAGFEIGGPQWQFTERADLFAGFDYALGVDGIA
MLLIMLSVFLMPICVLASWEAIQKRVGEYMAALLLTEMLMIGVFAAQDLFLFYIFFEAGLIPMYLLIGIWGGADRIYAAF
KFFLYTLIGSVLMLVAMFWMVNEAGTSDIPTLMQYDFAPEAQTWLWLAFFASFAVKVPMFPFHTWLPAAHVQAPTAGSVI
LAGVLLKLGGYGFIRFSLPMFPEASAQFTWLVFALSMIAVIYTSLVALVQEDMKKLIAYSSVAHMAIVTAALFAFNVQGL
EGAMIVMLSHGLVSGALFLCVGIIYDRLHTREIGRYGGLAINMPKYALFFLLFTMASIGLPGTSGFVAEFLSLAGTYQVS
SSVTLFLTTGIILGAGYMLYLYRRVVFGTQVNEDAAAMKDLNLREWAMLGPIAAAVLWMGVYPESFLAPMRQDIAALDAR
LSRAAPEGDAKLAAGEPRAEAANAVAYEAKEGGAH
>Mature_515_residues
MEGFPILTALLVVPLVAALACYFLEASAARMVALVATMINLALGIALWAGFEIGGPQWQFTERADLFAGFDYALGVDGIA
MLLIMLSVFLMPICVLASWEAIQKRVGEYMAALLLTEMLMIGVFAAQDLFLFYIFFEAGLIPMYLLIGIWGGADRIYAAF
KFFLYTLIGSVLMLVAMFWMVNEAGTSDIPTLMQYDFAPEAQTWLWLAFFASFAVKVPMFPFHTWLPAAHVQAPTAGSVI
LAGVLLKLGGYGFIRFSLPMFPEASAQFTWLVFALSMIAVIYTSLVALVQEDMKKLIAYSSVAHMAIVTAALFAFNVQGL
EGAMIVMLSHGLVSGALFLCVGIIYDRLHTREIGRYGGLAINMPKYALFFLLFTMASIGLPGTSGFVAEFLSLAGTYQVS
SSVTLFLTTGIILGAGYMLYLYRRVVFGTQVNEDAAAMKDLNLREWAMLGPIAAAVLWMGVYPESFLAPMRQDIAALDAR
LSRAAPEGDAKLAAGEPRAEAANAVAYEAKEGGAH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=366, Percent_Identity=35.792349726776, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI251831117, Length=437, Percent_Identity=23.3409610983982, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788613, Length=496, Percent_Identity=37.0967741935484, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1788827, Length=294, Percent_Identity=29.2517006802721, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1788831, Length=295, Percent_Identity=28.135593220339, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788614, Length=359, Percent_Identity=28.6908077994429, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI2367154, Length=249, Percent_Identity=25.7028112449799, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55930; Mature: 55930

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCC
>Mature Secondary Structure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HHHCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]