Click here to switch to the map view.

The map label for this gene is bkdA2 [H]

Identifier: 85373859

GI number: 85373859

Start: 1069220

End: 1070251

Strand: Direct

Name: bkdA2 [H]

Synonym: ELI_05160

Alternate gene names: 85373859

Gene position: 1069220-1070251 (Clockwise)

Preceding gene: 85373858

Following gene: 85373860

Centisome position: 35.03

GC content: 60.95

Gene sequence:

>1032_bases
GTGGCCGAGCAGGGCACCGAACGCCGCCTCAACATGATCGAGGCGATCAACGATGCGCTCGACATCATGCTGGAGCGCGA
TCCCGATGTCATCATAATGGGTGAGGACGTCGGCTATTTCGGCGGCGTGTTCCGGTGTACTGCGGGCCTGCAGGAAAAGC
ACGGCAAGACGCGGGTCTTCGATACGCCGATCAGCGAATGCGGGATCATCGGCGTCGCCGTAGGGATGGGGGCATATGGC
CTGCGCCCAGTGCCTGAAATCCAGTTTGCCGACTACATTTATCCGGGCCTCGACCAGCTGATCTCCGAAGCTGCTCGCCT
GCGCTATCGCTCGGCCTGCGACTACATCGCGCCGATGACGGTACGCTCGCCATTCGGGGGGGGCATTTTCGGCGGGCAGA
CGCACAGCCAATCGCCCGAGGCGATCTTCACGCATGTGTCCGGCCTGAAGACTGTGATCCCATCGACGCCTTATGATGCC
AAGGGCCTGCTGATCAGCTGTATCGAAGACAACGATCCGGTGATCTTCTTCGAGCCGAAGCGTATCTATAACGGCCCATT
TTCTGGCTTCTACGACAAGCCGGTCGAACCGTGGAAGAAGCATAAGGATAGCGTGGTCCCCGAAGGGCATTACACGATAC
CGCTTGGCAAGGCGCGCCATGTGACCGAGGGCGAAGAACTGACCGTGCTGGCCTACGGCACGATGGTTCATGTCGCCGAG
GCTGTATGCCGCGAGAAGGGCGTCGAGGCCGACATCCTCGACCTGCGCACCATGGTGCCGCTCGACATCGAAGCGATCGA
AGCGTCGGTCAAGAAGACCGGTCGGTGCATGATCGTCCACGAGGCGACGCGCACATCCGGCTTCGGGGCCGAGCTTTCCG
CGCTGGTCCAGGAACGCTGTTTCTATCACCTCGAAGCCCCGATCGAACGCGTCACCGGCTTCGACACACCCTATCCGCAC
AGCCTCGAATGGGCCTATTTCCCCGGCCCCGTTCGCCTTGGCGAAGCAATTGACCGACTTTTGAAGGACTGA

Upstream 100 bases:

>100_bases
AGCAGGCCGCGCAGGCCAAGCGCGAACGCGAGATCAAATGGCCCGGTGAGGAGCCCTGGTCATGAGCGACGCCTTAAAAA
CACACGCGAGGGAAAAGCCT

Downstream 100 bases:

>100_bases
CCCGATGGCAAAGTTCACTTTCAACATGCCCGACGTGGGCGAGGGCGTAGCCGAGGCGGAGATCGTCGAATGGCACGTTA
AGGTCGGTGATACGGTGTCC

Product: 2-oxoisovalerate dehydrogenase (beta subunit)

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MAEQGTERRLNMIEAINDALDIMLERDPDVIIMGEDVGYFGGVFRCTAGLQEKHGKTRVFDTPISECGIIGVAVGMGAYG
LRPVPEIQFADYIYPGLDQLISEAARLRYRSACDYIAPMTVRSPFGGGIFGGQTHSQSPEAIFTHVSGLKTVIPSTPYDA
KGLLISCIEDNDPVIFFEPKRIYNGPFSGFYDKPVEPWKKHKDSVVPEGHYTIPLGKARHVTEGEELTVLAYGTMVHVAE
AVCREKGVEADILDLRTMVPLDIEAIEASVKKTGRCMIVHEATRTSGFGAELSALVQERCFYHLEAPIERVTGFDTPYPH
SLEWAYFPGPVRLGEAIDRLLKD

Sequences:

>Translated_343_residues
MAEQGTERRLNMIEAINDALDIMLERDPDVIIMGEDVGYFGGVFRCTAGLQEKHGKTRVFDTPISECGIIGVAVGMGAYG
LRPVPEIQFADYIYPGLDQLISEAARLRYRSACDYIAPMTVRSPFGGGIFGGQTHSQSPEAIFTHVSGLKTVIPSTPYDA
KGLLISCIEDNDPVIFFEPKRIYNGPFSGFYDKPVEPWKKHKDSVVPEGHYTIPLGKARHVTEGEELTVLAYGTMVHVAE
AVCREKGVEADILDLRTMVPLDIEAIEASVKKTGRCMIVHEATRTSGFGAELSALVQERCFYHLEAPIERVTGFDTPYPH
SLEWAYFPGPVRLGEAIDRLLKD
>Mature_342_residues
AEQGTERRLNMIEAINDALDIMLERDPDVIIMGEDVGYFGGVFRCTAGLQEKHGKTRVFDTPISECGIIGVAVGMGAYGL
RPVPEIQFADYIYPGLDQLISEAARLRYRSACDYIAPMTVRSPFGGGIFGGQTHSQSPEAIFTHVSGLKTVIPSTPYDAK
GLLISCIEDNDPVIFFEPKRIYNGPFSGFYDKPVEPWKKHKDSVVPEGHYTIPLGKARHVTEGEELTVLAYGTMVHVAEA
VCREKGVEADILDLRTMVPLDIEAIEASVKKTGRCMIVHEATRTSGFGAELSALVQERCFYHLEAPIERVTGFDTPYPHS
LEWAYFPGPVRLGEAIDRLLKD

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=345, Percent_Identity=46.6666666666667, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI34101272, Length=345, Percent_Identity=46.6666666666667, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI156564403, Length=312, Percent_Identity=38.1410256410256, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI291084858, Length=312, Percent_Identity=36.8589743589744, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17506935, Length=342, Percent_Identity=46.1988304093567, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17538422, Length=348, Percent_Identity=35.0574712643678, Blast_Score=200, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6319698, Length=344, Percent_Identity=33.7209302325581, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI160714828, Length=345, Percent_Identity=44.0579710144928, Blast_Score=294, Evalue=5e-80,
Organism=Drosophila melanogaster, GI160714832, Length=343, Percent_Identity=44.3148688046647, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI21358145, Length=340, Percent_Identity=35.8823529411765, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24650940, Length=340, Percent_Identity=35.8823529411765, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI160714826, Length=73, Percent_Identity=47.945205479452, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=40.6593406593407, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=40.6593406593407, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 37902; Mature: 37771

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQGTERRLNMIEAINDALDIMLERDPDVIIMGEDVGYFGGVFRCTAGLQEKHGKTRVF
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHCCCCEEE
DTPISECGIIGVAVGMGAYGLRPVPEIQFADYIYPGLDQLISEAARLRYRSACDYIAPMT
ECCHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHCCCE
VRSPFGGGIFGGQTHSQSPEAIFTHVSGLKTVIPSTPYDAKGLLISCIEDNDPVIFFEPK
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEECCH
RIYNGPFSGFYDKPVEPWKKHKDSVVPEGHYTIPLGKARHVTEGEELTVLAYGTMVHVAE
HHCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEHHHHHHHH
AVCREKGVEADILDLRTMVPLDIEAIEASVKKTGRCMIVHEATRTSGFGAELSALVQERC
HHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHH
FYHLEAPIERVTGFDTPYPHSLEWAYFPGPVRLGEAIDRLLKD
HHHHHCHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AEQGTERRLNMIEAINDALDIMLERDPDVIIMGEDVGYFGGVFRCTAGLQEKHGKTRVF
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHCCCCEEE
DTPISECGIIGVAVGMGAYGLRPVPEIQFADYIYPGLDQLISEAARLRYRSACDYIAPMT
ECCHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHCCCE
VRSPFGGGIFGGQTHSQSPEAIFTHVSGLKTVIPSTPYDAKGLLISCIEDNDPVIFFEPK
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEECCH
RIYNGPFSGFYDKPVEPWKKHKDSVVPEGHYTIPLGKARHVTEGEELTVLAYGTMVHVAE
HHCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEHHHHHHHH
AVCREKGVEADILDLRTMVPLDIEAIEASVKKTGRCMIVHEATRTSGFGAELSALVQERC
HHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHH
FYHLEAPIERVTGFDTPYPHSLEWAYFPGPVRLGEAIDRLLKD
HHHHHCHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]