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The map label for this gene is bkdB [H]

Identifier: 85373860

GI number: 85373860

Start: 1070256

End: 1071566

Strand: Direct

Name: bkdB [H]

Synonym: ELI_05165

Alternate gene names: 85373860

Gene position: 1070256-1071566 (Clockwise)

Preceding gene: 85373859

Following gene: 85373862

Centisome position: 35.06

GC content: 62.7

Gene sequence:

>1311_bases
ATGGCAAAGTTCACTTTCAACATGCCCGACGTGGGCGAGGGCGTAGCCGAGGCGGAGATCGTCGAATGGCACGTTAAGGT
CGGTGATACGGTGTCCGAAGACCAGCATCTGGTCGACGTGATGACCGACAAGGCGACGATCGACATCGAAAGTCCGGTCG
ATGGCAAGGTGCTCGAACTGGCCGGAGAGCCGGGCGATGTAACCGCGGTGGGTGCGATGTTGCTCGTGATCGAAGTCGAA
GGCGAAGTGCCGGACGAGGTCGAGGAGGCCAATGAGGAAGCAGCAGCTGCAGCTGAACCCGCTCCCGCTCCGAAATCCGA
ACCGGTCGAAGAACGCATCGAAGTCGAAAATCCCGATGCGAGCGACGCGGACGATGCGATGGCGGCCGATCCTGAACCGG
CACCTGCGCAGCCGGTCGAGGAAGCGCCCTCTGCCAAGGCCGAAACCAAGGTCCTTGCCAGTCCTGCCGTGCGCAAGCGG
GCCAAGGAGCTCGGCATCGACCTCAGCGAAGTGAAACCTGCCGAAGACGGACGCATCCGCCACGGCGATCTCGACGCTTT
CCTGTCCTACACCGGCGGTTTCTCCGCTGCCGCTCCGACGCGCAGCGATGAAGAGTTGAAAGTCATCGGCATGCGTCGCC
GCATTGCCGAGAACATGGCGGCCTCCAAGCGCAACATCCCGCATTTCTCCTATGTTGAAGAAGTCGATGTGACCGACCTC
GAGACCATGCGCGCGCAGCTCAACGCCAATCGCGGCGAGAAGCCCAAGCTTACGATCCTGCCGCTGCTGATAACCGCGAT
CTGCAAGACGCTGCCGGACTTCCCGATGATCAACGCTCGTTACGACGATGAGGGCGGGGTCGTGACCCGACATGGCGCGG
TCCATCTCGGGATGGCGACACAGACCGATGCAGGGCTGATGGTGCCGGTGATCCGCGACGCGCAGTCAAGGAACCTGTGG
CAGCTTGCCAAGGAAATCGGGCGCCTGGCCGAGGCTGCCCGCACCGGCAAGGCAAAGTCGGAGGAATTGTCCGGCGGCAC
GCTGACCGTCACCTCGCTCGGCCCGCTCGGCGGTGTTGCGACGACGCCGGTCATCAATCGCCCGGAAGTCGCGATCATCG
GGCCTAACCGGATCATTGAGCGCCCGATGTTCGTGCCCGACGGGCAGGGCGGCGAGCGGATCGAAAAGCGCAAGCTGATG
AATATCTCCATCAGTTGCGACCACCGCGTGGTCGACGGCTACGACGCGGCGAGCTTCGTCCAGGCGCTCAAGAAATTGAT
CGAGACGCCGACTTTGCTCTTGGTCGACTGA

Upstream 100 bases:

>100_bases
GGCTTCGACACACCCTATCCGCACAGCCTCGAATGGGCCTATTTCCCCGGCCCCGTTCGCCTTGGCGAAGCAATTGACCG
ACTTTTGAAGGACTGACCCG

Downstream 100 bases:

>100_bases
TCAGCGAACTATTGCGCTGTAGGTCAGGACACCGCGGCCAAGCGCGGCTTCGGCGTTGCTGACCTGCCAGCGCAGGTGCG
TGACGTCCTCAGGCGAAGCG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAKFTFNMPDVGEGVAEAEIVEWHVKVGDTVSEDQHLVDVMTDKATIDIESPVDGKVLELAGEPGDVTAVGAMLLVIEVE
GEVPDEVEEANEEAAAAAEPAPAPKSEPVEERIEVENPDASDADDAMAADPEPAPAQPVEEAPSAKAETKVLASPAVRKR
AKELGIDLSEVKPAEDGRIRHGDLDAFLSYTGGFSAAAPTRSDEELKVIGMRRRIAENMAASKRNIPHFSYVEEVDVTDL
ETMRAQLNANRGEKPKLTILPLLITAICKTLPDFPMINARYDDEGGVVTRHGAVHLGMATQTDAGLMVPVIRDAQSRNLW
QLAKEIGRLAEAARTGKAKSEELSGGTLTVTSLGPLGGVATTPVINRPEVAIIGPNRIIERPMFVPDGQGGERIEKRKLM
NISISCDHRVVDGYDAASFVQALKKLIETPTLLLVD

Sequences:

>Translated_436_residues
MAKFTFNMPDVGEGVAEAEIVEWHVKVGDTVSEDQHLVDVMTDKATIDIESPVDGKVLELAGEPGDVTAVGAMLLVIEVE
GEVPDEVEEANEEAAAAAEPAPAPKSEPVEERIEVENPDASDADDAMAADPEPAPAQPVEEAPSAKAETKVLASPAVRKR
AKELGIDLSEVKPAEDGRIRHGDLDAFLSYTGGFSAAAPTRSDEELKVIGMRRRIAENMAASKRNIPHFSYVEEVDVTDL
ETMRAQLNANRGEKPKLTILPLLITAICKTLPDFPMINARYDDEGGVVTRHGAVHLGMATQTDAGLMVPVIRDAQSRNLW
QLAKEIGRLAEAARTGKAKSEELSGGTLTVTSLGPLGGVATTPVINRPEVAIIGPNRIIERPMFVPDGQGGERIEKRKLM
NISISCDHRVVDGYDAASFVQALKKLIETPTLLLVD
>Mature_435_residues
AKFTFNMPDVGEGVAEAEIVEWHVKVGDTVSEDQHLVDVMTDKATIDIESPVDGKVLELAGEPGDVTAVGAMLLVIEVEG
EVPDEVEEANEEAAAAAEPAPAPKSEPVEERIEVENPDASDADDAMAADPEPAPAQPVEEAPSAKAETKVLASPAVRKRA
KELGIDLSEVKPAEDGRIRHGDLDAFLSYTGGFSAAAPTRSDEELKVIGMRRRIAENMAASKRNIPHFSYVEEVDVTDLE
TMRAQLNANRGEKPKLTILPLLITAICKTLPDFPMINARYDDEGGVVTRHGAVHLGMATQTDAGLMVPVIRDAQSRNLWQ
LAKEIGRLAEAARTGKAKSEELSGGTLTVTSLGPLGGVATTPVINRPEVAIIGPNRIIERPMFVPDGQGGERIEKRKLMN
ISISCDHRVVDGYDAASFVQALKKLIETPTLLLVD

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=462, Percent_Identity=30.5194805194805, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI31711992, Length=444, Percent_Identity=27.7027027027027, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI19923748, Length=238, Percent_Identity=31.0924369747899, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI203098816, Length=231, Percent_Identity=29.8701298701299, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI203098753, Length=231, Percent_Identity=29.8701298701299, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI260898739, Length=170, Percent_Identity=31.1764705882353, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1786946, Length=450, Percent_Identity=27.7777777777778, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1786305, Length=303, Percent_Identity=29.042904290429, Blast_Score=148, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=450, Percent_Identity=31.1111111111111, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17560088, Length=435, Percent_Identity=29.8850574712644, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=440, Percent_Identity=27.7272727272727, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=240, Percent_Identity=27.0833333333333, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320352, Length=434, Percent_Identity=29.0322580645161, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=465, Percent_Identity=25.3763440860215, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859875, Length=456, Percent_Identity=31.5789473684211, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24645909, Length=237, Percent_Identity=31.2236286919831, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24582497, Length=293, Percent_Identity=26.2798634812287, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI20129315, Length=293, Percent_Identity=26.2798634812287, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 46749; Mature: 46618

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFTFNMPDVGEGVAEAEIVEWHVKVGDTVSEDQHLVDVMTDKATIDIESPVDGKVLEL
CCEEECCCCCCCCCCHHCEEEEEEEECCCCCCCCHHHHHEECCCEEEEECCCCCCCEEEE
AGEPGDVTAVGAMLLVIEVEGEVPDEVEEANEEAAAAAEPAPAPKSEPVEERIEVENPDA
CCCCCCHHEEEEEEEEEEECCCCCHHHHHHCHHHHHCCCCCCCCCCCCHHHHHCCCCCCC
SDADDAMAADPEPAPAQPVEEAPSAKAETKVLASPAVRKRAKELGIDLSEVKPAEDGRIR
CCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCCEE
HGDLDAFLSYTGGFSAAAPTRSDEELKVIGMRRRIAENMAASKRNIPHFSYVEEVDVTDL
CCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHH
ETMRAQLNANRGEKPKLTILPLLITAICKTLPDFPMINARYDDEGGVVTRHGAVHLGMAT
HHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCEEEEEECC
QTDAGLMVPVIRDAQSRNLWQLAKEIGRLAEAARTGKAKSEELSGGTLTVTSLGPLGGVA
CCCCCEEEHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCC
TTPVINRPEVAIIGPNRIIERPMFVPDGQGGERIEKRKLMNISISCDHRVVDGYDAASFV
CCCCCCCCCEEEECCHHHHHCCCCCCCCCCCHHHHHHHEEEEEEECCCEEECCCCHHHHH
QALKKLIETPTLLLVD
HHHHHHHCCCCEEEEC
>Mature Secondary Structure 
AKFTFNMPDVGEGVAEAEIVEWHVKVGDTVSEDQHLVDVMTDKATIDIESPVDGKVLEL
CEEECCCCCCCCCCHHCEEEEEEEECCCCCCCCHHHHHEECCCEEEEECCCCCCCEEEE
AGEPGDVTAVGAMLLVIEVEGEVPDEVEEANEEAAAAAEPAPAPKSEPVEERIEVENPDA
CCCCCCHHEEEEEEEEEEECCCCCHHHHHHCHHHHHCCCCCCCCCCCCHHHHHCCCCCCC
SDADDAMAADPEPAPAQPVEEAPSAKAETKVLASPAVRKRAKELGIDLSEVKPAEDGRIR
CCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCCEE
HGDLDAFLSYTGGFSAAAPTRSDEELKVIGMRRRIAENMAASKRNIPHFSYVEEVDVTDL
CCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHH
ETMRAQLNANRGEKPKLTILPLLITAICKTLPDFPMINARYDDEGGVVTRHGAVHLGMAT
HHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCEEEEEECC
QTDAGLMVPVIRDAQSRNLWQLAKEIGRLAEAARTGKAKSEELSGGTLTVTSLGPLGGVA
CCCCCEEEHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCC
TTPVINRPEVAIIGPNRIIERPMFVPDGQGGERIEKRKLMNISISCDHRVVDGYDAASFV
CCCCCCCCCEEEECCHHHHHCCCCCCCCCCCHHHHHHHEEEEEEECCCEEECCCCHHHHH
QALKKLIETPTLLLVD
HHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]