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The map label for this gene is lepB [H]

Identifier: 85373796

GI number: 85373796

Start: 999280

End: 1000101

Strand: Reverse

Name: lepB [H]

Synonym: ELI_04845

Alternate gene names: 85373796

Gene position: 1000101-999280 (Counterclockwise)

Preceding gene: 85373799

Following gene: 85373795

Centisome position: 32.76

GC content: 64.84

Gene sequence:

>822_bases
ATGAATGAAAAGGCCCAAGCTGTTACGAATACGGATGCGCCCACGAAGCGCACGTGGCTCAGCTTTCCGGCCTTCCTAGT
GCTGGTGGTGCTGGCCGCCCTCGCCTTCCGCAGTTTCGCCTTTTCGCTGTTCAGCATTCCCAGCGAGAGCATGCTGCCGC
GGCTGGTGATCGGCGACTACCTGATCGCGTCGAAATGGGATTATGGCATTTCCAATGCGTCGCTGCCGTTCGACCTGCCG
CTGATCCCGGGGCGTTTCTTCGCCGAGCAGCCCGAGCGCGGCGATGTGGTGATCTTCAAGCATCCGATCGACGGGGCGGA
TTACGTCAAGCGCGTCATCGGCCTGCCGGGCGACGTGGTCGAACTGGACGGCGGCGAAGTCGTGCTCAATGGCGAGCGCC
TGCCGCAGGAAAAGCTCAGCGATTTCGTGGTGCCGGTCAGCCCCAATACCCGCTGCGCCTGGGGCGCAGAGGAAGAACGG
CTGGCCGACGGCCACCTGCAATGCCGCTACACCCGTTTGCGCGAAACGCTGCCCAGCGGGCGCGATTACGAAGTGCTGGA
CTTTGGTCCCACCCCGCAGGACAGCTACGATCCGGTCCTCGTGCCCGAGGGCATGCTGTTCCTGCTGGGCGACAATCGCG
ACAATTCGCAGGACAGCCGCTTCCCGCCCGAAGCGCAGGGCGGCATTGGGCTGCTCTCGCAGGGCCTGCTGGTCGGCAGG
GCGCGCCGCGTCCTGTTCTCGACCGATGGCGGCGCCGAATGGCTCAAGCCCTGGACATGGTTCACGGCAGCTCGCGGCGA
GCGTATGGGGAACGCCCTATGA

Upstream 100 bases:

>100_bases
TTTTCCACCCGGTGGATCGCATCCCATGCGTTGGTTTCAGCATCGCTCATCGCGCAAATTCCTCTTGCCAGCCTTCCCGT
CGCGCCTAGGGGTGCCAGCG

Downstream 100 bases:

>100_bases
CGATTCTCGCACCCGAAACCCTGCAATGGCTCGCCGATACCGGTTTCGCGGTGGAAGACGACGAGCTGTGGCTGGAAGCC
TTGACGCACGGCAGCACCGG

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNEKAQAVTNTDAPTKRTWLSFPAFLVLVVLAALAFRSFAFSLFSIPSESMLPRLVIGDYLIASKWDYGISNASLPFDLP
LIPGRFFAEQPERGDVVIFKHPIDGADYVKRVIGLPGDVVELDGGEVVLNGERLPQEKLSDFVVPVSPNTRCAWGAEEER
LADGHLQCRYTRLRETLPSGRDYEVLDFGPTPQDSYDPVLVPEGMLFLLGDNRDNSQDSRFPPEAQGGIGLLSQGLLVGR
ARRVLFSTDGGAEWLKPWTWFTAARGERMGNAL

Sequences:

>Translated_273_residues
MNEKAQAVTNTDAPTKRTWLSFPAFLVLVVLAALAFRSFAFSLFSIPSESMLPRLVIGDYLIASKWDYGISNASLPFDLP
LIPGRFFAEQPERGDVVIFKHPIDGADYVKRVIGLPGDVVELDGGEVVLNGERLPQEKLSDFVVPVSPNTRCAWGAEEER
LADGHLQCRYTRLRETLPSGRDYEVLDFGPTPQDSYDPVLVPEGMLFLLGDNRDNSQDSRFPPEAQGGIGLLSQGLLVGR
ARRVLFSTDGGAEWLKPWTWFTAARGERMGNAL
>Mature_273_residues
MNEKAQAVTNTDAPTKRTWLSFPAFLVLVVLAALAFRSFAFSLFSIPSESMLPRLVIGDYLIASKWDYGISNASLPFDLP
LIPGRFFAEQPERGDVVIFKHPIDGADYVKRVIGLPGDVVELDGGEVVLNGERLPQEKLSDFVVPVSPNTRCAWGAEEER
LADGHLQCRYTRLRETLPSGRDYEVLDFGPTPQDSYDPVLVPEGMLFLLGDNRDNSQDSRFPPEAQGGIGLLSQGLLVGR
ARRVLFSTDGGAEWLKPWTWFTAARGERMGNAL

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=298, Percent_Identity=30.8724832214765, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 30201; Mature: 30201

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKAQAVTNTDAPTKRTWLSFPAFLVLVVLAALAFRSFAFSLFSIPSESMLPRLVIGDY
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHCC
LIASKWDYGISNASLPFDLPLIPGRFFAEQPERGDVVIFKHPIDGADYVKRVIGLPGDVV
CEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEE
ELDGGEVVLNGERLPQEKLSDFVVPVSPNTRCAWGAEEERLADGHLQCRYTRLRETLPSG
EECCCEEEECCCCCCHHHHHCEEEECCCCCCEECCCCHHHHCCCCCEEHHHHHHHHCCCC
RDYEVLDFGPTPQDSYDPVLVPEGMLFLLGDNRDNSQDSRFPPEAQGGIGLLSQGLLVGR
CCEEEEECCCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCHHHHC
ARRVLFSTDGGAEWLKPWTWFTAARGERMGNAL
CEEEEEECCCCHHHCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNEKAQAVTNTDAPTKRTWLSFPAFLVLVVLAALAFRSFAFSLFSIPSESMLPRLVIGDY
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHCC
LIASKWDYGISNASLPFDLPLIPGRFFAEQPERGDVVIFKHPIDGADYVKRVIGLPGDVV
CEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEE
ELDGGEVVLNGERLPQEKLSDFVVPVSPNTRCAWGAEEERLADGHLQCRYTRLRETLPSG
EECCCEEEECCCCCCHHHHHCEEEECCCCCCEECCCCHHHHCCCCCEEHHHHHHHHCCCC
RDYEVLDFGPTPQDSYDPVLVPEGMLFLLGDNRDNSQDSRFPPEAQGGIGLLSQGLLVGR
CCEEEEECCCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCHHHHC
ARRVLFSTDGGAEWLKPWTWFTAARGERMGNAL
CEEEEEECCCCHHHCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA