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The map label for this gene is murI [H]

Identifier: 85373785

GI number: 85373785

Start: 990029

End: 990799

Strand: Reverse

Name: murI [H]

Synonym: ELI_04790

Alternate gene names: 85373785

Gene position: 990799-990029 (Counterclockwise)

Preceding gene: 85373794

Following gene: 85373784

Centisome position: 32.46

GC content: 64.85

Gene sequence:

>771_bases
TTGTTCGACACCGGTGTCGGCGGGCTGACGGTGCTGGCCGAACTGCGCAAGGTGCTGCCCAATGCGCCGGTGATCTATGC
CGCCGACTATGCCGGCCTGCCCTATGGCACCAAGACCGAGGCGGAAGTTGCCGCGCGGGTCTGCGGCCTGCTGGGCCGGA
TGAGCGAACGCTATCAACCGCGCGCCATCTGTATTGCCTGTAATACCGCCAGCACCATTGCGCTCGGCATGGTGAGGGAA
GTTCTCGAAGTACCGATCATCGGCACGGTGCCCGCCATCAAGCCGGCTGCCGCAATGACCGAGACCGGCGTAATCGGCCT
GCTCGGCACGGCTGCGACCGTGCGCCAGCCCTATGTCGACCGGCTCGAAGCGGAATTCGCCAGCGACACGGTGCTGATCC
GCTTCGCCGCTTCGGGCCTCGTGGACCTGGCCGAGGCCAAGCTGCGCGGCGAGACAGTGGGGACGAATAGCCTGCGCGCG
GAGGTACGCGCTCTGCTGGAAGCGGAGAACGCCGACAGGATCGACACGCTCGTCCTCGCCTGCACCCATTTTCCGCTGCT
CGAAGAAGAGTTGTTGCAAGTTTTCGGCCCGCAGGTGCGAATGGTGCATGGTGCCGAAGGTATCGCCCGCCGCATCGCCG
CCGTCACCCACGGCCAGCAGTTCGCGCGTACCACTCCGGATCGTTTCATCACGACCGGCAGCGCGAAGGATGCAGCGGAC
CTCGCACATTGTCTGTCGCAATACGGTCTCGACTCGATCGAGGCATTCTGA

Upstream 100 bases:

>100_bases
TGCCCTTCGTCTTTGACCTTGCTCTAAAAGTAAGTACGGCCCTTCGACAAGCTCAGGGCGAACGGAAATCTCTATTTTGG
ACGCGTCATCTCCCATCCTG

Downstream 100 bases:

>100_bases
TGCGAATCATTCGCAAAGATCGCGCTGGATAGCGGCGGCTTTTGGCATTATCGGCCCTCATCGAGCTCATGAATTACGAC
CAGATCTTCGACCAGGCGAT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MFDTGVGGLTVLAELRKVLPNAPVIYAADYAGLPYGTKTEAEVAARVCGLLGRMSERYQPRAICIACNTASTIALGMVRE
VLEVPIIGTVPAIKPAAAMTETGVIGLLGTAATVRQPYVDRLEAEFASDTVLIRFAASGLVDLAEAKLRGETVGTNSLRA
EVRALLEAENADRIDTLVLACTHFPLLEEELLQVFGPQVRMVHGAEGIARRIAAVTHGQQFARTTPDRFITTGSAKDAAD
LAHCLSQYGLDSIEAF

Sequences:

>Translated_256_residues
MFDTGVGGLTVLAELRKVLPNAPVIYAADYAGLPYGTKTEAEVAARVCGLLGRMSERYQPRAICIACNTASTIALGMVRE
VLEVPIIGTVPAIKPAAAMTETGVIGLLGTAATVRQPYVDRLEAEFASDTVLIRFAASGLVDLAEAKLRGETVGTNSLRA
EVRALLEAENADRIDTLVLACTHFPLLEEELLQVFGPQVRMVHGAEGIARRIAAVTHGQQFARTTPDRFITTGSAKDAAD
LAHCLSQYGLDSIEAF
>Mature_256_residues
MFDTGVGGLTVLAELRKVLPNAPVIYAADYAGLPYGTKTEAEVAARVCGLLGRMSERYQPRAICIACNTASTIALGMVRE
VLEVPIIGTVPAIKPAAAMTETGVIGLLGTAATVRQPYVDRLEAEFASDTVLIRFAASGLVDLAEAKLRGETVGTNSLRA
EVRALLEAENADRIDTLVLACTHFPLLEEELLQVFGPQVRMVHGAEGIARRIAAVTHGQQFARTTPDRFITTGSAKDAAD
LAHCLSQYGLDSIEAF

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=256, Percent_Identity=41.40625, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 27212; Mature: 27212

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDTGVGGLTVLAELRKVLPNAPVIYAADYAGLPYGTKTEAEVAARVCGLLGRMSERYQP
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
RAICIACNTASTIALGMVREVLEVPIIGTVPAIKPAAAMTETGVIGLLGTAATVRQPYVD
CEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
RLEAEFASDTVLIRFAASGLVDLAEAKLRGETVGTNSLRAEVRALLEAENADRIDTLVLA
HHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
CTHFPLLEEELLQVFGPQVRMVHGAEGIARRIAAVTHGQQFARTTPDRFITTGSAKDAAD
HHCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCHHHHHCCCCCEEECCCCCHHHH
LAHCLSQYGLDSIEAF
HHHHHHHHCCHHHCCC
>Mature Secondary Structure
MFDTGVGGLTVLAELRKVLPNAPVIYAADYAGLPYGTKTEAEVAARVCGLLGRMSERYQP
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
RAICIACNTASTIALGMVREVLEVPIIGTVPAIKPAAAMTETGVIGLLGTAATVRQPYVD
CEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
RLEAEFASDTVLIRFAASGLVDLAEAKLRGETVGTNSLRAEVRALLEAENADRIDTLVLA
HHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
CTHFPLLEEELLQVFGPQVRMVHGAEGIARRIAAVTHGQQFARTTPDRFITTGSAKDAAD
HHCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCHHHHHCCCCCEEECCCCCHHHH
LAHCLSQYGLDSIEAF
HHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA