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The map label for this gene is hupB [H]

Identifier: 85373566

GI number: 85373566

Start: 764916

End: 765188

Strand: Direct

Name: hupB [H]

Synonym: ELI_03695

Alternate gene names: 85373566

Gene position: 764916-765188 (Clockwise)

Preceding gene: 85373565

Following gene: 85373570

Centisome position: 25.06

GC content: 60.44

Gene sequence:

>273_bases
ATGAACAAGAACGATCTGATCAGCGCGGTTGCCGATGCCAGCGGCCTTTCCAAGAGTGACGCTTCGGGCGCCGTCGAAGG
CGTTTTCGACTCGATTACCAAGGCCCTGTCGAACGGCGATGAAGTGCGTCTGGTCGGGTTCGGCACGTTCTCCGTCGCGC
GCCGCAAGGCTTCGACCGGTCGGAACCCGCGTACCGGCGAGCCGATGACGATCAAGGCTTCCAACCAGCCGAAATTCAAG
GCCGGCAAGGGTCTGAAAGACGCCGTCAATTAA

Upstream 100 bases:

>100_bases
TAATCGCCCTAGACAGAACACAGAAAAAAGAGTGTGATGGAAAGTCTTGATGGAGGCGATTCATCAGCTTCAAGACTTCG
AAAGAGGGGGTTTTCCTGAA

Downstream 100 bases:

>100_bases
GCAAGAGCGCGGATAACGGGTCGAGCCTCATCGCCAGGCTCGTGTATAGCAACCCCGCTCACCTCACGGTGGGCGGGGTT
CTTCGTATGCGGCCGGGTGT

Product: histone-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKNDLISAVADASGLSKSDASGAVEGVFDSITKALSNGDEVRLVGFGTFSVARRKASTGRNPRTGEPMTIKASNQPKFK
AGKGLKDAVN

Sequences:

>Translated_90_residues
MNKNDLISAVADASGLSKSDASGAVEGVFDSITKALSNGDEVRLVGFGTFSVARRKASTGRNPRTGEPMTIKASNQPKFK
AGKGLKDAVN
>Mature_90_residues
MNKNDLISAVADASGLSKSDASGAVEGVFDSITKALSNGDEVRLVGFGTFSVARRKASTGRNPRTGEPMTIKASNQPKFK
AGKGLKDAVN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=52.2222222222222, Blast_Score=97, Evalue=3e-22,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=51.685393258427, Blast_Score=95, Evalue=9e-22,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=40.4494382022472, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=34.0659340659341, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9314; Mature: 9314

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNDLISAVADASGLSKSDASGAVEGVFDSITKALSNGDEVRLVGFGTFSVARRKASTG
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCC
RNPRTGEPMTIKASNQPKFKAGKGLKDAVN
CCCCCCCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKNDLISAVADASGLSKSDASGAVEGVFDSITKALSNGDEVRLVGFGTFSVARRKASTG
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCC
RNPRTGEPMTIKASNQPKFKAGKGLKDAVN
CCCCCCCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6299736; 10762258; 11481430; 7049166 [H]