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The map label for this gene is kefA [C]

Identifier: 85373206

GI number: 85373206

Start: 424275

End: 425510

Strand: Reverse

Name: kefA [C]

Synonym: ELI_01895

Alternate gene names: 85373206

Gene position: 425510-424275 (Counterclockwise)

Preceding gene: 85373207

Following gene: 85373205

Centisome position: 13.94

GC content: 61.57

Gene sequence:

>1236_bases
ATGACCACTTCCGCGCCTTCCACGACCGTGTCCGATACTGCACCTGCGCCGAGTCCATCGCCTTCGCCGGAGGCCGTTCC
TCTCGTCGATCCGGAAACCGGCGAGCCCCTGCTCGATCCCGAAACGGGGGAGCAGCTCACAGGCATTCCGGCGCCGCAGC
CGACCGAATATCTCGACCCGGAAACCGGCGAGGTCCTGGTCGACCCGGAAACCGGTGAGCCTCTCGCGGCCGAGCCTGAA
GCCGTTGCGACGCCGGAACCGACGCCGACCGAGGCAATCACCACGGCCGAGGAGGCCAAGGCAGCCGTGGCCGAGAAAAG
CGAGACGGTCGGCAGCATGATCGAATCGATGGACGCGATGGCGATCGAGCTTGGCTCGATGCGTCTCTCGCTATGGGACA
TCGTGTTCGCGTCGGCGATCGTCTTGCTGATCATCCTCGGCGCGTGGATCGCGACCAAGGCGATCCACGCCCTCGTTACG
CGGGTCACGCGCCTCGACGACACACAGCGGCTCCTGTCCGAAAAGATCGTCACGATCGTCGTCTGGGCGGCAGCCTTTTT
CCTCGGCATTGACCTGCTCGGCATCGACCTGACGGCGCTCGCGGTGTTTTCCGGTGCCTTCGGTCTGGCGATCGGTTTCG
GCCTGCAGAAGACCTTCGGCAACCTGATTGCCGGGATCATCCTTCTGATGGACAAGTCCATCAAACCCGGCGACGTAATC
GCGGTATCGGACCAGGCCGGCAATTCGACCTTCGGCCAGATCCAGAAAATCGGCATCCGCGCGGTTTCGATCACGACGCG
CGACCAGAAGGAATACCTGATTCCGAACGAGAACCTGATGATCAACCAGGTCGAAAACTGGTCCTATTCGAGCAAGAACG
TGCGTATGCAGGTGCCGGTCGGGGTGAGCTATAATTGCGATCTGAAACTGGCCGAAAAGCTGATGCTGGAAGCAGCCAAG
AGCAGCAAGCGCGTCAAGAAATCGCCCCCGCCCACCGCTTGGCTCGACGAATATGGCGACAGTTCGGTCAACTTCATCAT
CCACTGCTGGATCTCGGACCCGGAAGACGGCGTCGGCAACGTAAAGTCGGAAGTGCTCAAGCGGTTGTGGGACCTGTTCG
AGGAACACGAGGTCGAAATCCCCTTCCCGCAGCGCGATCTCAACCTGCGCGGCAATGCACAGTTCGACCAGCTCGTCGCC
GCGATCACGCAGCGGGTGAAAAGCGACAAAGCGTAG

Upstream 100 bases:

>100_bases
CCGTAGACGAGCACCTCGGCATTCGGCTATCGATCGGGCTCGAAGACCCCGAAGACCTGATCGCCGATCTCGATCAGGCG
TTCACTGCCATGGGAACTGC

Downstream 100 bases:

>100_bases
CTTCCGCCCCGCAAACGCGGGCTTTCAAAATCTTAGCTACCTCCCCTGCGACCCATTCTCGCTGGCAGGACGAACGGTGC
AGGGGCATCTCGCCTGCATG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTTSAPSTTVSDTAPAPSPSPSPEAVPLVDPETGEPLLDPETGEQLTGIPAPQPTEYLDPETGEVLVDPETGEPLAAEPE
AVATPEPTPTEAITTAEEAKAAVAEKSETVGSMIESMDAMAIELGSMRLSLWDIVFASAIVLLIILGAWIATKAIHALVT
RVTRLDDTQRLLSEKIVTIVVWAAAFFLGIDLLGIDLTALAVFSGAFGLAIGFGLQKTFGNLIAGIILLMDKSIKPGDVI
AVSDQAGNSTFGQIQKIGIRAVSITTRDQKEYLIPNENLMINQVENWSYSSKNVRMQVPVGVSYNCDLKLAEKLMLEAAK
SSKRVKKSPPPTAWLDEYGDSSVNFIIHCWISDPEDGVGNVKSEVLKRLWDLFEEHEVEIPFPQRDLNLRGNAQFDQLVA
AITQRVKSDKA

Sequences:

>Translated_411_residues
MTTSAPSTTVSDTAPAPSPSPSPEAVPLVDPETGEPLLDPETGEQLTGIPAPQPTEYLDPETGEVLVDPETGEPLAAEPE
AVATPEPTPTEAITTAEEAKAAVAEKSETVGSMIESMDAMAIELGSMRLSLWDIVFASAIVLLIILGAWIATKAIHALVT
RVTRLDDTQRLLSEKIVTIVVWAAAFFLGIDLLGIDLTALAVFSGAFGLAIGFGLQKTFGNLIAGIILLMDKSIKPGDVI
AVSDQAGNSTFGQIQKIGIRAVSITTRDQKEYLIPNENLMINQVENWSYSSKNVRMQVPVGVSYNCDLKLAEKLMLEAAK
SSKRVKKSPPPTAWLDEYGDSSVNFIIHCWISDPEDGVGNVKSEVLKRLWDLFEEHEVEIPFPQRDLNLRGNAQFDQLVA
AITQRVKSDKA
>Mature_410_residues
TTSAPSTTVSDTAPAPSPSPSPEAVPLVDPETGEPLLDPETGEQLTGIPAPQPTEYLDPETGEVLVDPETGEPLAAEPEA
VATPEPTPTEAITTAEEAKAAVAEKSETVGSMIESMDAMAIELGSMRLSLWDIVFASAIVLLIILGAWIATKAIHALVTR
VTRLDDTQRLLSEKIVTIVVWAAAFFLGIDLLGIDLTALAVFSGAFGLAIGFGLQKTFGNLIAGIILLMDKSIKPGDVIA
VSDQAGNSTFGQIQKIGIRAVSITTRDQKEYLIPNENLMINQVENWSYSSKNVRMQVPVGVSYNCDLKLAEKLMLEAAKS
SKRVKKSPPPTAWLDEYGDSSVNFIIHCWISDPEDGVGNVKSEVLKRLWDLFEEHEVEIPFPQRDLNLRGNAQFDQLVAA
ITQRVKSDKA

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=199, Percent_Identity=30.1507537688442, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789291, Length=279, Percent_Identity=26.8817204301075, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI2367355, Length=200, Percent_Identity=27.5, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787591, Length=235, Percent_Identity=23.8297872340426, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 44305; Mature: 44174

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSAPSTTVSDTAPAPSPSPSPEAVPLVDPETGEPLLDPETGEQLTGIPAPQPTEYLDP
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ETGEVLVDPETGEPLAAEPEAVATPEPTPTEAITTAEEAKAAVAEKSETVGSMIESMDAM
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIELGSMRLSLWDIVFASAIVLLIILGAWIATKAIHALVTRVTRLDDTQRLLSEKIVTIV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VWAAAFFLGIDLLGIDLTALAVFSGAFGLAIGFGLQKTFGNLIAGIILLMDKSIKPGDVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
AVSDQAGNSTFGQIQKIGIRAVSITTRDQKEYLIPNENLMINQVENWSYSSKNVRMQVPV
EEECCCCCCHHHHHHHHCEEEEEEEECCCCCEECCCCCEEEEEECCCCCCCCCEEEEECC
GVSYNCDLKLAEKLMLEAAKSSKRVKKSPPPTAWLDEYGDSSVNFIIHCWISDPEDGVGN
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCCCCH
VKSEVLKRLWDLFEEHEVEIPFPQRDLNLRGNAQFDQLVAAITQRVKSDKA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTSAPSTTVSDTAPAPSPSPSPEAVPLVDPETGEPLLDPETGEQLTGIPAPQPTEYLDP
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ETGEVLVDPETGEPLAAEPEAVATPEPTPTEAITTAEEAKAAVAEKSETVGSMIESMDAM
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIELGSMRLSLWDIVFASAIVLLIILGAWIATKAIHALVTRVTRLDDTQRLLSEKIVTIV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VWAAAFFLGIDLLGIDLTALAVFSGAFGLAIGFGLQKTFGNLIAGIILLMDKSIKPGDVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
AVSDQAGNSTFGQIQKIGIRAVSITTRDQKEYLIPNENLMINQVENWSYSSKNVRMQVPV
EEECCCCCCHHHHHHHHCEEEEEEEECCCCCEECCCCCEEEEEECCCCCCCCCEEEEECC
GVSYNCDLKLAEKLMLEAAKSSKRVKKSPPPTAWLDEYGDSSVNFIIHCWISDPEDGVGN
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCCCCH
VKSEVLKRLWDLFEEHEVEIPFPQRDLNLRGNAQFDQLVAAITQRVKSDKA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]