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The map label for this gene is cysG [H]

Identifier: 85373205

GI number: 85373205

Start: 423419

End: 424177

Strand: Reverse

Name: cysG [H]

Synonym: ELI_01890

Alternate gene names: 85373205

Gene position: 424177-423419 (Counterclockwise)

Preceding gene: 85373206

Following gene: 85373204

Centisome position: 13.9

GC content: 67.06

Gene sequence:

>759_bases
ATGGCAGAAACTGGCACCATCTATCTCGTCGGCGCAGGTCCGGGCGATCCTGACCTGCTGACGCTGCGCGCGGCGCGATT
GCTCGAGCGCGCTGGACTGATCGTCCATGATGGTCTCGTCGACCCTTCGATCCTTGCACTGGCCCGGCCCGACGCAACGC
TCGTGTCGGTCGCCAAGCAGCGCAGCAAGCACACATTGCCGCAGGAGGACATCAACGCCCTGCTCGTGCGCGAAGCGAGG
GCCGGACGCGATGTCGTACGGCTCAAAGGCGGCGATCCGCTGATCTTCGGGCGCGGCGGCGAGGAAGCCGAAGCTGCGCA
GGCCGCGGGTGTTCCGGTCGAGATCGTCCCCGGGATCAGCGCGGCCAACGGGGCCGCTGCTGCCGCACAGATCGCCCTCA
CCCATCGCGACGCGTCCAGCATCGTCAGCTTCGTTGCGGGCCAGTGCAAGGGTCTGAAGGACCAGGACTGGGCCGGTCTT
GCCGGCAAGGGACGCACGCTGGTGATCTACATGGGCGTCAAGACGGCACCGCAGATTGCCGAGAAATTGATGGCCGACGG
CCTTTCGCCCGACATGCCCATCGCTGTGATCGAAAATGGCGCGCGGCCGGAGATGCGCGTGCTGCGCGGCCTGCTGGCGG
GGCTCCCCGGTCTCGTCGAGGCTGAGCGTGTGAAGAGCCCCGCCCTGATCGTCATCGGCGATGTTACCGCGCGCGACGAC
GCCCGTATCGCGCAAATCGCCACGGAGGCAGCAGAATGA

Upstream 100 bases:

>100_bases
TAGCTTCCGCCCCGCAAACGCGGGCTTTCAAAATCTTAGCTACCTCCCCTGCGACCCATTCTCGCTGGCAGGACGAACGG
TGCAGGGGCATCTCGCCTGC

Downstream 100 bases:

>100_bases
AGATCCTCACCGGCAACGATCTCAAGACCGGCGCGGTCGTCTGGTGGGACGGCACCGGCTGGTCGCTCCATGTCAACGAT
GCCGTCGATACCGGCGAGCG

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MAETGTIYLVGAGPGDPDLLTLRAARLLERAGLIVHDGLVDPSILALARPDATLVSVAKQRSKHTLPQEDINALLVREAR
AGRDVVRLKGGDPLIFGRGGEEAEAAQAAGVPVEIVPGISAANGAAAAAQIALTHRDASSIVSFVAGQCKGLKDQDWAGL
AGKGRTLVIYMGVKTAPQIAEKLMADGLSPDMPIAVIENGARPEMRVLRGLLAGLPGLVEAERVKSPALIVIGDVTARDD
ARIAQIATEAAE

Sequences:

>Translated_252_residues
MAETGTIYLVGAGPGDPDLLTLRAARLLERAGLIVHDGLVDPSILALARPDATLVSVAKQRSKHTLPQEDINALLVREAR
AGRDVVRLKGGDPLIFGRGGEEAEAAQAAGVPVEIVPGISAANGAAAAAQIALTHRDASSIVSFVAGQCKGLKDQDWAGL
AGKGRTLVIYMGVKTAPQIAEKLMADGLSPDMPIAVIENGARPEMRVLRGLLAGLPGLVEAERVKSPALIVIGDVTARDD
ARIAQIATEAAE
>Mature_251_residues
AETGTIYLVGAGPGDPDLLTLRAARLLERAGLIVHDGLVDPSILALARPDATLVSVAKQRSKHTLPQEDINALLVREARA
GRDVVRLKGGDPLIFGRGGEEAEAAQAAGVPVEIVPGISAANGAAAAAQIALTHRDASSIVSFVAGQCKGLKDQDWAGLA
GKGRTLVIYMGVKTAPQIAEKLMADGLSPDMPIAVIENGARPEMRVLRGLLAGLPGLVEAERVKSPALIVIGDVTARDDA
RIAQIATEAAE

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=236, Percent_Identity=47.0338983050847, Blast_Score=212, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=33.1932773109244, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 26031; Mature: 25900

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETGTIYLVGAGPGDPDLLTLRAARLLERAGLIVHDGLVDPSILALARPDATLVSVAKQ
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHEEEECCCHHHHHHHHH
RSKHTLPQEDINALLVREARAGRDVVRLKGGDPLIFGRGGEEAEAAQAAGVPVEIVPGIS
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHHHHHCCCCEEEECCCC
AANGAAAAAQIALTHRDASSIVSFVAGQCKGLKDQDWAGLAGKGRTLVIYMGVKTAPQIA
CCCCCHHHEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
EKLMADGLSPDMPIAVIENGARPEMRVLRGLLAGLPGLVEAERVKSPALIVIGDVTARDD
HHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCCCC
ARIAQIATEAAE
HHHHHHHHHHCC
>Mature Secondary Structure 
AETGTIYLVGAGPGDPDLLTLRAARLLERAGLIVHDGLVDPSILALARPDATLVSVAKQ
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHEEEECCCHHHHHHHHH
RSKHTLPQEDINALLVREARAGRDVVRLKGGDPLIFGRGGEEAEAAQAAGVPVEIVPGIS
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHHHHHCCCCEEEECCCC
AANGAAAAAQIALTHRDASSIVSFVAGQCKGLKDQDWAGLAGKGRTLVIYMGVKTAPQIA
CCCCCHHHEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
EKLMADGLSPDMPIAVIENGARPEMRVLRGLLAGLPGLVEAERVKSPALIVIGDVTARDD
HHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCCCC
ARIAQIATEAAE
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA