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The map label for this gene is caiD [H]

Identifier: 85372837

GI number: 85372837

Start: 19608

End: 20393

Strand: Direct

Name: caiD [H]

Synonym: ELI_00050

Alternate gene names: 85372837

Gene position: 19608-20393 (Clockwise)

Preceding gene: 85372834

Following gene: 85372838

Centisome position: 0.64

GC content: 63.74

Gene sequence:

>786_bases
ATGAGCGACACCGACACGCAGGCCCAGACCGAGGAAGTCCTGACCGAAACCCGCGACGGCATCCTGATCATCACCATCAA
CCGGCCCGAGGCCAAGAACGCCATGACCAAGGCGGCATCCGAAGGGATCGCCGCGGCGCTCGACCGGCTCGACAGCGAGG
ACGACCTGCGCGTCGGCATCCTGACCGGGGCGGGCGGCACTTTCTGTTCGGGCATGGATCTGAAGGGCTTCCTGCGCGGC
GAAACGCCGAGTGTCCCGGGGCGCGGTTTCGGCGGGCTGACCGAGCAATCCCCGGCCAAACCGCTTATCGCAGCGGTCGA
AGGCTATGCTCTGGCAGGCGGGATGGAACTGATGATCAGCTGCGACCTGATTGTCGCGAACTCAGGCTCCAAGTTCGGTA
TTCCCGAAGCCAAGCGTGGACTGGTCGCGGCGGCTGGCGGCCTGATGAAGCTGCCCGATCTGATCCCACCGAAAGTGGCG
ATGGAGCTGGCGCTGACCGGGGACTTTATCGATTCCGCGCGCGCCTATGAATTGGGCCTGGTCAACCGCGTGACCGATGG
GTCCGCGCTCGATGCGGCGATCGAATTGGCGCAGGCGATTACCGCCAATGGCCCGCTCGCAGTCAAGGTTTCCAAGCAGA
TCATCGAGGAATCGCGTGGCTGGCCGCTGGAAGAGCGGTGGAAAGAACAGGGCAAGCTGATGCCGCAGATATTCATGAGC
GAAGATGCACGTGAAGGCGCGGCAGCCTTCGCGGAAAAGCGCGCGCCCAACTGGAAGGGCAAGTAA

Upstream 100 bases:

>100_bases
GAATCATTGTGCCGGACCAAGAGTCCGGTTGACGTTTTCGTAAAGCTTGGCCAGCACCGGCACAAGAAAAGTTTCAATTT
GCCATGGAGAGGCGATCCTT

Downstream 100 bases:

>100_bases
CGATGGCGGGTCCGCTCAACGGCATCCGCATCGTCGAATTCGCCGGGATCGGGCCGGGTCCGTTCTGCGGCATGATGCTG
GCCGACCACGGCGCGGAGGT

Product: enoyl CoA dehydratase/isomerase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSDTDTQAQTEEVLTETRDGILIITINRPEAKNAMTKAASEGIAAALDRLDSEDDLRVGILTGAGGTFCSGMDLKGFLRG
ETPSVPGRGFGGLTEQSPAKPLIAAVEGYALAGGMELMISCDLIVANSGSKFGIPEAKRGLVAAAGGLMKLPDLIPPKVA
MELALTGDFIDSARAYELGLVNRVTDGSALDAAIELAQAITANGPLAVKVSKQIIEESRGWPLEERWKEQGKLMPQIFMS
EDAREGAAAFAEKRAPNWKGK

Sequences:

>Translated_261_residues
MSDTDTQAQTEEVLTETRDGILIITINRPEAKNAMTKAASEGIAAALDRLDSEDDLRVGILTGAGGTFCSGMDLKGFLRG
ETPSVPGRGFGGLTEQSPAKPLIAAVEGYALAGGMELMISCDLIVANSGSKFGIPEAKRGLVAAAGGLMKLPDLIPPKVA
MELALTGDFIDSARAYELGLVNRVTDGSALDAAIELAQAITANGPLAVKVSKQIIEESRGWPLEERWKEQGKLMPQIFMS
EDAREGAAAFAEKRAPNWKGK
>Mature_260_residues
SDTDTQAQTEEVLTETRDGILIITINRPEAKNAMTKAASEGIAAALDRLDSEDDLRVGILTGAGGTFCSGMDLKGFLRGE
TPSVPGRGFGGLTEQSPAKPLIAAVEGYALAGGMELMISCDLIVANSGSKFGIPEAKRGLVAAAGGLMKLPDLIPPKVAM
ELALTGDFIDSARAYELGLVNRVTDGSALDAAIELAQAITANGPLAVKVSKQIIEESRGWPLEERWKEQGKLMPQIFMSE
DAREGAAAFAEKRAPNWKGK

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=32.3076923076923, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=31.6602316602317, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=28.2868525896414, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI68989263, Length=281, Percent_Identity=24.9110320284698, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI70995211, Length=262, Percent_Identity=28.2442748091603, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI150378541, Length=249, Percent_Identity=30.5220883534137, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=25.8823529411765, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=25.8823529411765, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=25.8823529411765, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=28.4090909090909, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=29.7435897435897, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI37594471, Length=195, Percent_Identity=29.7435897435897, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=25.531914893617, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=25.531914893617, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=43.2950191570881, Blast_Score=171, Evalue=3e-44,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=31.2741312741313, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=30.9160305343511, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=29.6577946768061, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=29.0196078431373, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788682, Length=190, Percent_Identity=32.6315789473684, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790281, Length=265, Percent_Identity=27.9245283018868, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=34.5679012345679, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=32.3886639676113, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17540714, Length=241, Percent_Identity=33.195020746888, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17560910, Length=261, Percent_Identity=30.6513409961686, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=258, Percent_Identity=25.1937984496124, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=232, Percent_Identity=26.7241379310345, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=233, Percent_Identity=27.8969957081545, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=28.4916201117318, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=22.7488151658768, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=195, Percent_Identity=25.6410256410256, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=36.4661654135338, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=33.7164750957854, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=33.7164750957854, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21357171, Length=197, Percent_Identity=31.9796954314721, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=29.8039215686275, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24650670, Length=218, Percent_Identity=32.5688073394495, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19920382, Length=229, Percent_Identity=27.5109170305677, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921000, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583077, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583079, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24654903, Length=193, Percent_Identity=24.3523316062176, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27489; Mature: 27358

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTDTQAQTEEVLTETRDGILIITINRPEAKNAMTKAASEGIAAALDRLDSEDDLRVGI
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LTGAGGTFCSGMDLKGFLRGETPSVPGRGFGGLTEQSPAKPLIAAVEGYALAGGMELMIS
EECCCCCEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCEEEEEE
CDLIVANSGSKFGIPEAKRGLVAAAGGLMKLPDLIPPKVAMELALTGDFIDSARAYELGL
EEEEEECCCCCCCCCHHHCCHHHHCCCCHHCCCCCCCHHHHHHHHCCCHHHCCHHHHHHH
VNRVTDGSALDAAIELAQAITANGPLAVKVSKQIIEESRGWPLEERWKEQGKLMPQIFMS
HHCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCHHHHHHHHCCCCHHHHHC
EDAREGAAAFAEKRAPNWKGK
CCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDTDTQAQTEEVLTETRDGILIITINRPEAKNAMTKAASEGIAAALDRLDSEDDLRVGI
CCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LTGAGGTFCSGMDLKGFLRGETPSVPGRGFGGLTEQSPAKPLIAAVEGYALAGGMELMIS
EECCCCCEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCEEEEEE
CDLIVANSGSKFGIPEAKRGLVAAAGGLMKLPDLIPPKVAMELALTGDFIDSARAYELGL
EEEEEECCCCCCCCCHHHCCHHHHCCCCHHCCCCCCCHHHHHHHHCCCHHHCCHHHHHHH
VNRVTDGSALDAAIELAQAITANGPLAVKVSKQIIEESRGWPLEERWKEQGKLMPQIFMS
HHCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCHHHHHHHHCCCCHHHHHC
EDAREGAAAFAEKRAPNWKGK
CCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA