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The map label for this gene is rep [H]

Identifier: 85060368

GI number: 85060368

Start: 4096352

End: 4098379

Strand: Reverse

Name: rep [H]

Synonym: SG2390

Alternate gene names: 85060368

Gene position: 4098379-4096352 (Counterclockwise)

Preceding gene: 85060369

Following gene: 85060365

Centisome position: 98.26

GC content: 56.61

Gene sequence:

>2028_bases
ATGCGCCTTAATCCAAGCCAACAACAAGCCGTCGAATTCGTCACCGGACCCTGTCTGGTGCTGGCCGGCGCCGGCTCGGG
CAAGACGCGGGTTATCACCAATAAAATCGCCCATCTGATCCGCGGTTGCGGCTATCAGGTGCGGCATATCGCGGCGGTGA
CGTTTACCAATAAAGCCTCCAGGGAGATGAAGGAGCGGGTGGCGCAAACGCTCGGTCGTGCAGAGGCGCGCGGGCTGACC
GTCGCCACGTTCCATACGCTCGGACTGGCTATCATCAAGCGCGAATATAAAGCGCTGGGCATGAAGGCCAAATTCTCTCT
ATTTGATGAGCAAGACCAACTAGCGCTGCTAAAGGATCTCTCCGAGCAGTGGCTGGAAGGGGACAAAGACCTGCTCAAGT
CGCTGACTTCCGCCATTTCCAACTGGAAGAACGATTTGCTGTCGCCGGCGCAGGCGGCCGACGCCGCCCGTTCCGAACGG
GATAAGCTGTTCGCCCATTGCTATGCGCTCTATGACGACCATCTCATCGCCTTCAATGTGTTGGATTTCGATGATTTGAT
TCTAAGGCCGACCCAGCTGTTGCAGCGCGACGAGGCGGTGCGCGAGCGCTGGCAAAACCGGCTGCGCTATTTGCTGGTGG
ATGAGTATCAGGATACCAATACCAGCCAGTATGAACTGGTGAAGCTGCTGGTAGGGCCGCGGGGCCGTTTCACCGTGGTG
GGCGACGACGATCAATCGATCTATTCCTGGCGCGGCGCGCGGCCGCAAAATCTGGCGTTGCTGCAACAAGATTTCCCGAC
GCTTGCGGTTATCAAGCTGGAGCAGAATTATCGCTCCACCGAGCGCATCCTGAATGCCGCCAATATTCTTATCGCCAACA
ATCCACATGTCTTTGAAAAGCGTCTTTTTTCCCGGCTGGGCTATGGCGACATCCTGAAAGTCATCACCGCCAATAATGAA
GACCACGAGGCTGAGCGGGTGGTGGGGGAGCTGATCGCCCACCACTTCATCAACAAGACCCGTTATCGGGATTACGCCAT
TTTGTATCGCGGCAATCACCAGTCGCGGCTGTTTGAAAAGATGCTGATGCAAAACCGCATTCCTTATCGCATCTCCGGCG
GGACGTCGTTTTTTTCACGGCCGGAGATTAAGGACTTGCTCGCCTATCTGCGCGTGCTGACCAATCCCGATGACGATAGC
GCGTTTTTACGGATAGTGAACACGCCACGCCGAGAGATAGGCGCGGCGACGCTGGCCAAATTGGGCACCTGGGCGACGCA
GCGCGATAAAAGCTTGTTTACTGCCAGTTTTGATTTGGGCCTTGGGCAAACGCTTAGCGGCCGCGGCCTTGAGTCGCTGC
AACAGTTTACTCACTGGCTGGATGGCGTCGCCCGTCAGGTGGAACGAGAGCCGGTGGCAGCAGTGCGTGAACTTATCCAC
GGCATCGACTATGAAAGCTGGTTGTATGAGAGTTCCACCAGTCCGAAAGCGGCGGAAATGCGAATGAAAAATGTTAATCA
ACTGTTCAGTTGGATGACCGAAATGCTTACCGGTTCCGATTTGAATGAGGCGATGACGCTGGATCAGGTGGTCACCCGAT
TCACTCTGCGCGATATGATGGAACGTGGCGAGAGCGAGGAGGAACTCGATCAGGTGCAGCTGATGACGTTGCATGCCTCC
AAGGGGCTGGAGTTTCCTTATGTGTTCCTGGTCGGCATGGAAGAGGGGTTATTGCCGCATCAAGGCAGTATCGATGAGAA
CAATATCGAAGAGGAGCGCCGGCTGGCTTATGTCGGCATTACCCGCGCGCAAAAGGAATTAACCTTCACCCTGTGCCGCG
AGCGGCGTCAATACGGTGAACTGGTCAAGCCAGAGCCGAGCCGCTTCCTGCTTGAATTACCTCAGGACGATGTGCAATGG
GAGCAGATACGCAAACCGGTTAGCGCCGAGGAGCGCATGAGCCGCGGCCAGGGGCATTTGGCGAGCCTGCGCGCCCAACT
GGCCAAGGCACGCGGCGAGGCCGACTAA

Upstream 100 bases:

>100_bases
TCCGGCGGGGGGGGGGGCCGCCGTGGGGATAAACCCCGGCGCCGCTTTCTGCTACAATCCCCCTCCGTTCTCCTCCATCG
TTTTTGCCCGGGATTCATTT

Downstream 100 bases:

>100_bases
CTTTCCCCACGCTGAGCGGGTAAGCGGTTAGGCGGGCGGCATCCTCAGGGCACTTCCTGCGGCAGAACGGTTGACGGTGA
GGGGAGAGCAGGCGGCTCAA

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 675; Mature: 675

Protein sequence:

>675_residues
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQVRHIAAVTFTNKASREMKERVAQTLGRAEARGLT
VATFHTLGLAIIKREYKALGMKAKFSLFDEQDQLALLKDLSEQWLEGDKDLLKSLTSAISNWKNDLLSPAQAADAARSER
DKLFAHCYALYDDHLIAFNVLDFDDLILRPTQLLQRDEAVRERWQNRLRYLLVDEYQDTNTSQYELVKLLVGPRGRFTVV
GDDDQSIYSWRGARPQNLALLQQDFPTLAVIKLEQNYRSTERILNAANILIANNPHVFEKRLFSRLGYGDILKVITANNE
DHEAERVVGELIAHHFINKTRYRDYAILYRGNHQSRLFEKMLMQNRIPYRISGGTSFFSRPEIKDLLAYLRVLTNPDDDS
AFLRIVNTPRREIGAATLAKLGTWATQRDKSLFTASFDLGLGQTLSGRGLESLQQFTHWLDGVARQVEREPVAAVRELIH
GIDYESWLYESSTSPKAAEMRMKNVNQLFSWMTEMLTGSDLNEAMTLDQVVTRFTLRDMMERGESEEELDQVQLMTLHAS
KGLEFPYVFLVGMEEGLLPHQGSIDENNIEEERRLAYVGITRAQKELTFTLCRERRQYGELVKPEPSRFLLELPQDDVQW
EQIRKPVSAEERMSRGQGHLASLRAQLAKARGEAD

Sequences:

>Translated_675_residues
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQVRHIAAVTFTNKASREMKERVAQTLGRAEARGLT
VATFHTLGLAIIKREYKALGMKAKFSLFDEQDQLALLKDLSEQWLEGDKDLLKSLTSAISNWKNDLLSPAQAADAARSER
DKLFAHCYALYDDHLIAFNVLDFDDLILRPTQLLQRDEAVRERWQNRLRYLLVDEYQDTNTSQYELVKLLVGPRGRFTVV
GDDDQSIYSWRGARPQNLALLQQDFPTLAVIKLEQNYRSTERILNAANILIANNPHVFEKRLFSRLGYGDILKVITANNE
DHEAERVVGELIAHHFINKTRYRDYAILYRGNHQSRLFEKMLMQNRIPYRISGGTSFFSRPEIKDLLAYLRVLTNPDDDS
AFLRIVNTPRREIGAATLAKLGTWATQRDKSLFTASFDLGLGQTLSGRGLESLQQFTHWLDGVARQVEREPVAAVRELIH
GIDYESWLYESSTSPKAAEMRMKNVNQLFSWMTEMLTGSDLNEAMTLDQVVTRFTLRDMMERGESEEELDQVQLMTLHAS
KGLEFPYVFLVGMEEGLLPHQGSIDENNIEEERRLAYVGITRAQKELTFTLCRERRQYGELVKPEPSRFLLELPQDDVQW
EQIRKPVSAEERMSRGQGHLASLRAQLAKARGEAD
>Mature_675_residues
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQVRHIAAVTFTNKASREMKERVAQTLGRAEARGLT
VATFHTLGLAIIKREYKALGMKAKFSLFDEQDQLALLKDLSEQWLEGDKDLLKSLTSAISNWKNDLLSPAQAADAARSER
DKLFAHCYALYDDHLIAFNVLDFDDLILRPTQLLQRDEAVRERWQNRLRYLLVDEYQDTNTSQYELVKLLVGPRGRFTVV
GDDDQSIYSWRGARPQNLALLQQDFPTLAVIKLEQNYRSTERILNAANILIANNPHVFEKRLFSRLGYGDILKVITANNE
DHEAERVVGELIAHHFINKTRYRDYAILYRGNHQSRLFEKMLMQNRIPYRISGGTSFFSRPEIKDLLAYLRVLTNPDDDS
AFLRIVNTPRREIGAATLAKLGTWATQRDKSLFTASFDLGLGQTLSGRGLESLQQFTHWLDGVARQVEREPVAAVRELIH
GIDYESWLYESSTSPKAAEMRMKNVNQLFSWMTEMLTGSDLNEAMTLDQVVTRFTLRDMMERGESEEELDQVQLMTLHAS
KGLEFPYVFLVGMEEGLLPHQGSIDENNIEEERRLAYVGITRAQKELTFTLCRERRQYGELVKPEPSRFLLELPQDDVQW
EQIRKPVSAEERMSRGQGHLASLRAQLAKARGEAD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=673, Percent_Identity=82.4665676077266, Blast_Score=1152, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=656, Percent_Identity=38.5670731707317, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1787196, Length=351, Percent_Identity=30.4843304843305, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=724, Percent_Identity=27.7624309392265, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324477, Length=687, Percent_Identity=21.5429403202329, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77100; Mature: 77100

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQVRHIAAVTFTNKAS
CCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHEEEEEECCHHH
REMKERVAQTLGRAEARGLTVATFHTLGLAIIKREYKALGMKAKFSLFDEQDQLALLKDL
HHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHH
SEQWLEGDKDLLKSLTSAISNWKNDLLSPAQAADAARSERDKLFAHCYALYDDHLIAFNV
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
LDFDDLILRPTQLLQRDEAVRERWQNRLRYLLVDEYQDTNTSQYELVKLLVGPRGRFTVV
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCCCCEEEE
GDDDQSIYSWRGARPQNLALLQQDFPTLAVIKLEQNYRSTERILNAANILIANNPHVFEK
CCCCCHHHHCCCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCHHHHH
RLFSRLGYGDILKVITANNEDHEAERVVGELIAHHFINKTRYRDYAILYRGNHQSRLFEK
HHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLMQNRIPYRISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTPRREIGAATLAK
HHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHH
LGTWATQRDKSLFTASFDLGLGQTLSGRGLESLQQFTHWLDGVARQVEREPVAAVRELIH
HHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GIDYESWLYESSTSPKAAEMRMKNVNQLFSWMTEMLTGSDLNEAMTLDQVVTRFTLRDMM
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ERGESEEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQGSIDENNIEEERRLAYVGI
HCCCCHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHEEHEEH
TRAQKELTFTLCRERRQYGELVKPEPSRFLLELPQDDVQWEQIRKPVSAEERMSRGQGHL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCHHHHHCCCCHHHHHHCCCCHH
ASLRAQLAKARGEAD
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQVRHIAAVTFTNKAS
CCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHEEEEEECCHHH
REMKERVAQTLGRAEARGLTVATFHTLGLAIIKREYKALGMKAKFSLFDEQDQLALLKDL
HHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHH
SEQWLEGDKDLLKSLTSAISNWKNDLLSPAQAADAARSERDKLFAHCYALYDDHLIAFNV
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
LDFDDLILRPTQLLQRDEAVRERWQNRLRYLLVDEYQDTNTSQYELVKLLVGPRGRFTVV
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCCCCEEEE
GDDDQSIYSWRGARPQNLALLQQDFPTLAVIKLEQNYRSTERILNAANILIANNPHVFEK
CCCCCHHHHCCCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCHHHHH
RLFSRLGYGDILKVITANNEDHEAERVVGELIAHHFINKTRYRDYAILYRGNHQSRLFEK
HHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLMQNRIPYRISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTPRREIGAATLAK
HHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHH
LGTWATQRDKSLFTASFDLGLGQTLSGRGLESLQQFTHWLDGVARQVEREPVAAVRELIH
HHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GIDYESWLYESSTSPKAAEMRMKNVNQLFSWMTEMLTGSDLNEAMTLDQVVTRFTLRDMM
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ERGESEEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQGSIDENNIEEERRLAYVGI
HCCCCHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHEEHEEH
TRAQKELTFTLCRERRQYGELVKPEPSRFLLELPQDDVQWEQIRKPVSAEERMSRGQGHL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCHHHHHCCCCHHHHHHCCCCHH
ASLRAQLAKARGEAD
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]