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The map label for this gene is bioH

Identifier: 85060302

GI number: 85060302

Start: 3991647

End: 3992420

Strand: Reverse

Name: bioH

Synonym: SG2324

Alternate gene names: 85060302

Gene position: 3992420-3991647 (Counterclockwise)

Preceding gene: 85060304

Following gene: 85060298

Centisome position: 95.72

GC content: 64.08

Gene sequence:

>774_bases
ATGGTGTCACTGTTTTGGCAAACAACCGGTACCGGCGATCGCGATCTTGTGCTGCTGCACGGATGGGGATTGAATGCCGA
GGTCTGGTCTTACATTGTTCCGCGGCTGGCGACGCACTTTCGCCTGCACCTGGTGGATCTGCCGGGTTATGGCCGTAGCC
GCGGTTATGGCGCGCTGACCCTTGAGGAGATGGCGGAGGAAGTGGCGTCGCGAGCTCCCCACGGCGCGTTATGGCTGGGC
TGGTCACTTGGCGGACTGGTGGCGACGACGGTAGCGCGCCGGTGCCCACATGCGGTCGCGGGTTTGGTAACCGTTGCCTC
TTCCCCGCGTTTTTGCGCCGACGGTGACTGGCCGGGGATACGACCTGAGGTGCTGGAAGGGTTTGCGCGCGAGCTGCGCC
AGGATTTCACCCGCACCGTCAGCCGTTTTCTCGGTCTGCAAACGCTGGGAACGGCGTCCGCGCGTCAGGATACGCGCTGG
TTGAAGAGCGTGGTACTGGCGCACCCGGCGCCCGCGATCGAGGTTCTGACCGGCGGGCTGGCGCTACTGCGCACCAGCGA
CGTGCGCAAGGCGCTGGACCAACTTGACGTGCCGCTGCTGCGCCTATACGGCTATCTGGATGGTCTGGTGCCGCGTAAAG
TGGTCCCGCTGGTGGATGAATTGAGCACCGCCAGCCACAGCATTGTCTTTGCCGGCGCGGCGCATGCGCCGTTCATTTCC
CACCCGGTGCCGTTTTGCCAGGCGCTGTGCGATTTCAGTCAGCTCGAATCCTGA

Upstream 100 bases:

>100_bases
TGATGGCTAAACCAAAGTGGCTGTCGGCATAGCCAACAGAGGGCGCCGATTGTTAGCATGGTGTCATACTTCCTTGTTTC
CGACTTTTGGACAATAGCAA

Downstream 100 bases:

>100_bases
GCGTTGGCGGCCGGCAAGGGTTTAGCTATGAGACGGTTTAGGGCGCAGAGCGAGCAGATTCACTCGGTCGCTAACGATCG
CTAAGCGTTTCCAGCCAATA

Product: putative biotin biosynthesis protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MVSLFWQTTGTGDRDLVLLHGWGLNAEVWSYIVPRLATHFRLHLVDLPGYGRSRGYGALTLEEMAEEVASRAPHGALWLG
WSLGGLVATTVARRCPHAVAGLVTVASSPRFCADGDWPGIRPEVLEGFARELRQDFTRTVSRFLGLQTLGTASARQDTRW
LKSVVLAHPAPAIEVLTGGLALLRTSDVRKALDQLDVPLLRLYGYLDGLVPRKVVPLVDELSTASHSIVFAGAAHAPFIS
HPVPFCQALCDFSQLES

Sequences:

>Translated_257_residues
MVSLFWQTTGTGDRDLVLLHGWGLNAEVWSYIVPRLATHFRLHLVDLPGYGRSRGYGALTLEEMAEEVASRAPHGALWLG
WSLGGLVATTVARRCPHAVAGLVTVASSPRFCADGDWPGIRPEVLEGFARELRQDFTRTVSRFLGLQTLGTASARQDTRW
LKSVVLAHPAPAIEVLTGGLALLRTSDVRKALDQLDVPLLRLYGYLDGLVPRKVVPLVDELSTASHSIVFAGAAHAPFIS
HPVPFCQALCDFSQLES
>Mature_257_residues
MVSLFWQTTGTGDRDLVLLHGWGLNAEVWSYIVPRLATHFRLHLVDLPGYGRSRGYGALTLEEMAEEVASRAPHGALWLG
WSLGGLVATTVARRCPHAVAGLVTVASSPRFCADGDWPGIRPEVLEGFARELRQDFTRTVSRFLGLQTLGTASARQDTRW
LKSVVLAHPAPAIEVLTGGLALLRTSDVRKALDQLDVPLLRLYGYLDGLVPRKVVPLVDELSTASHSIVFAGAAHAPFIS
HPVPFCQALCDFSQLES

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=254, Percent_Identity=61.0236220472441, Blast_Score=318, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_SODGM (Q2NQH6)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_456004.1
- ProteinModelPortal:   Q2NQH6
- STRING:   Q2NQH6
- GeneID:   3867309
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2324
- NMPDR:   fig|343509.6.peg.5046
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   CTIASSP
- PhylomeDB:   Q2NQH6
- BioCyc:   SGLO343509:SG2324-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 27985; Mature: 27985

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: ACT_SITE 82-82 ACT_SITE 207-207 ACT_SITE 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLFWQTTGTGDRDLVLLHGWGLNAEVWSYIVPRLATHFRLHLVDLPGYGRSRGYGALT
CEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEE
LEEMAEEVASRAPHGALWLGWSLGGLVATTVARRCPHAVAGLVTVASSPRFCADGDWPGI
HHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCC
RPEVLEGFARELRQDFTRTVSRFLGLQTLGTASARQDTRWLKSVVLAHPAPAIEVLTGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCH
ALLRTSDVRKALDQLDVPLLRLYGYLDGLVPRKVVPLVDELSTASHSIVFAGAAHAPFIS
HHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
HPVPFCQALCDFSQLES
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSLFWQTTGTGDRDLVLLHGWGLNAEVWSYIVPRLATHFRLHLVDLPGYGRSRGYGALT
CEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEE
LEEMAEEVASRAPHGALWLGWSLGGLVATTVARRCPHAVAGLVTVASSPRFCADGDWPGI
HHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCC
RPEVLEGFARELRQDFTRTVSRFLGLQTLGTASARQDTRWLKSVVLAHPAPAIEVLTGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCH
ALLRTSDVRKALDQLDVPLLRLYGYLDGLVPRKVVPLVDELSTASHSIVFAGAAHAPFIS
HHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
HPVPFCQALCDFSQLES
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA