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The map label for this gene is greB [H]

Identifier: 85060298

GI number: 85060298

Start: 3983728

End: 3984195

Strand: Reverse

Name: greB [H]

Synonym: SG2320

Alternate gene names: 85060298

Gene position: 3984195-3983728 (Counterclockwise)

Preceding gene: 85060302

Following gene: 85060297

Centisome position: 95.52

GC content: 53.21

Gene sequence:

>468_bases
ATGAAGACCAACCTTATTACCCGCCAGGGCTATCAGGAATTGCAGGATGAGCTGGACCATTTGTGGAAACGCTACCGGCC
GGAGATTACCGAGAAGGTTGCCTGGGCCGCCAGTCTGGGCGATCGCAGTGAAAATGCGGATTATCATTACAATAAGAAAA
TTCTGCGCGAGATTGACCGTCGGGTGCGTTATTTGCGCCGTGTGCTGAAGGAGGCGCGGGTGGTGGATTACTCCCCGCAG
CAAGAGGGACGGATATTTTTCGGCGCCTGGGTGGAAGTGGAGAACGCCGAGGGGGAAACGAAACGCTTTCGTATCGCCGG
TCCCGATGAAATTTATAAACATAAGGACTATATCTCCATCGATTCACCCATGGCGCGCGCCTTGATCAAAAAGGCGGTGG
ATGATGAAGCGGAAGTGGTTACCCCCGGCGGACATCGCACCTGGTATATCAACGCCATCTCTTATTAA

Upstream 100 bases:

>100_bases
GGTTTGCGGCGGCAAAATGCCAGCCGCCCGCGTCCGTTCACCCCCGCCGCCGCTATGGTATATTGCCGTTGTTGGCCACA
GAGTTCGACGACAGGTGACG

Downstream 100 bases:

>100_bases
GGTTGACGTGCGCGGGGCGGTGGAGGAAACGTCGTGGCCGTTAATTATTATTCGCGGCGGCGCGACAGATGTTATTATCG
TGGAGATTACGTGCGGAAAG

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKTNLITRQGYQELQDELDHLWKRYRPEITEKVAWAASLGDRSENADYHYNKKILREIDRRVRYLRRVLKEARVVDYSPQ
QEGRIFFGAWVEVENAEGETKRFRIAGPDEIYKHKDYISIDSPMARALIKKAVDDEAEVVTPGGHRTWYINAISY

Sequences:

>Translated_155_residues
MKTNLITRQGYQELQDELDHLWKRYRPEITEKVAWAASLGDRSENADYHYNKKILREIDRRVRYLRRVLKEARVVDYSPQ
QEGRIFFGAWVEVENAEGETKRFRIAGPDEIYKHKDYISIDSPMARALIKKAVDDEAEVVTPGGHRTWYINAISY
>Mature_155_residues
MKTNLITRQGYQELQDELDHLWKRYRPEITEKVAWAASLGDRSENADYHYNKKILREIDRRVRYLRRVLKEARVVDYSPQ
QEGRIFFGAWVEVENAEGETKRFRIAGPDEIYKHKDYISIDSPMARALIKKAVDDEAEVVTPGGHRTWYINAISY

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=68.3870967741936, Blast_Score=226, Evalue=4e-61,
Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=35.6687898089172, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18333; Mature: 18333

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNLITRQGYQELQDELDHLWKRYRPEITEKVAWAASLGDRSENADYHYNKKILREIDR
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLRRVLKEARVVDYSPQQEGRIFFGAWVEVENAEGETKRFRIAGPDEIYKHKDYISI
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCHHHHHCCCCCCC
DSPMARALIKKAVDDEAEVVTPGGHRTWYINAISY
CCHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECC
>Mature Secondary Structure
MKTNLITRQGYQELQDELDHLWKRYRPEITEKVAWAASLGDRSENADYHYNKKILREIDR
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLRRVLKEARVVDYSPQQEGRIFFGAWVEVENAEGETKRFRIAGPDEIYKHKDYISI
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCHHHHHCCCCCCC
DSPMARALIKKAVDDEAEVVTPGGHRTWYINAISY
CCHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]