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The map label for this gene is sbp [H]

Identifier: 85060155

GI number: 85060155

Start: 3790489

End: 3791481

Strand: Reverse

Name: sbp [H]

Synonym: SG2177

Alternate gene names: 85060155

Gene position: 3791481-3790489 (Counterclockwise)

Preceding gene: 85060156

Following gene: 85060151

Centisome position: 90.9

GC content: 53.78

Gene sequence:

>993_bases
ATGTCCAAATGGCGTGCCGGCGTGTTACTGATGTTGCTGTCGGGAAGCGTAGCAGCAAAGGATATTCAGCTGCTGAATGT
TTCCTATGATCCTACCCGGGAATTCTATCAGCAATATAATAAGGCGTTTAGCCAGTACTGGCAGCAAAAAACCGGCGATC
ATGTTACGGTGCGCCAGTCCCACGGCGGCTCCGGCAAACAGGCGACTTCCGTCATTAACGGCATAGAGGCGGATGTGGTC
ACGCTGGCGCTGGAGTCGGATGTGGATGCTATCGCCGAACGCGGGCGCATCGATAAAAACTGGATTACGCGCCTGCCCGA
CAACTCCGCGCCTTACACCTCAACCATCGTTTTCCTGGTGCGCAAGGGCAATCCGAAACAAATTCATGATTGGTCCGATC
TGATTAAACCCGGCGTGTCGATCATTACCCCGAACCCGAAAACGTCCGGCGGGGCGCGCTGGAAGTATCTGGCCGCCTGG
GGCTATGCGCTGCACCAGAATAATAACGATCAGGCTAAAGCGCAGGCGTTTGTGAAAGTGTTGTTCAAGAATGTGGAAGT
TCTCGATTCCGGCGCGCGCGGTGCGACCAACACGTTTGTTGAGCGTGGCCAGGGGGATGTGCTCATCGCGTGGGAAAATG
AGGCGCTGTTGGCCATTAATGAGTTAAATAGCAAGGATCAGTTTGAAATCATAACCCCGAGTGAATCGATTTTGGCTGAG
CCGACGGTGTCGGTGGTGGATAAGGTGGTGGATAAGCGCGGCACCCGCGATGTGGCCACGGCCTATCTGAACTATCTCTA
TTCGACTGAAGGGCAACGTATTGCGGCGCAGAACTATTATCGCCCGCGGTACGCCAACGTGGCCAAAGAATATGCTAAAG
AGTTTCCAACCCTGAAGCTGTTTACTCTGGCTGAGACCTTCGGCGATTGGACCAAGGTGCAAAAAGAACATTTCGCCAGC
GGCGGCAGTTTTGATCAAATCAGCCAGCGTTGA

Upstream 100 bases:

>100_bases
TATATAAAATATTTTTATATTCTTTTAGATCGCTAAATTTTTCTGCCACTCTCTGCAGAAAATGTTTCCAATTCCAAAAA
ATTTCCATATGGGGTGAATA

Downstream 100 bases:

>100_bases
GCACAGGCGGGCGGGGCAACCGCCGCTTGACCTGGTGCTTTTGCGAGGGACTGCGTTACCCCCTCGGCGGGCTTGAGCTA
TCAGGACTGTTTCGCTTCTG

Product: sulfate transporter subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSKWRAGVLLMLLSGSVAAKDIQLLNVSYDPTREFYQQYNKAFSQYWQQKTGDHVTVRQSHGGSGKQATSVINGIEADVV
TLALESDVDAIAERGRIDKNWITRLPDNSAPYTSTIVFLVRKGNPKQIHDWSDLIKPGVSIITPNPKTSGGARWKYLAAW
GYALHQNNNDQAKAQAFVKVLFKNVEVLDSGARGATNTFVERGQGDVLIAWENEALLAINELNSKDQFEIITPSESILAE
PTVSVVDKVVDKRGTRDVATAYLNYLYSTEGQRIAAQNYYRPRYANVAKEYAKEFPTLKLFTLAETFGDWTKVQKEHFAS
GGSFDQISQR

Sequences:

>Translated_330_residues
MSKWRAGVLLMLLSGSVAAKDIQLLNVSYDPTREFYQQYNKAFSQYWQQKTGDHVTVRQSHGGSGKQATSVINGIEADVV
TLALESDVDAIAERGRIDKNWITRLPDNSAPYTSTIVFLVRKGNPKQIHDWSDLIKPGVSIITPNPKTSGGARWKYLAAW
GYALHQNNNDQAKAQAFVKVLFKNVEVLDSGARGATNTFVERGQGDVLIAWENEALLAINELNSKDQFEIITPSESILAE
PTVSVVDKVVDKRGTRDVATAYLNYLYSTEGQRIAAQNYYRPRYANVAKEYAKEFPTLKLFTLAETFGDWTKVQKEHFAS
GGSFDQISQR
>Mature_329_residues
SKWRAGVLLMLLSGSVAAKDIQLLNVSYDPTREFYQQYNKAFSQYWQQKTGDHVTVRQSHGGSGKQATSVINGIEADVVT
LALESDVDAIAERGRIDKNWITRLPDNSAPYTSTIVFLVRKGNPKQIHDWSDLIKPGVSIITPNPKTSGGARWKYLAAWG
YALHQNNNDQAKAQAFVKVLFKNVEVLDSGARGATNTFVERGQGDVLIAWENEALLAINELNSKDQFEIITPSESILAEP
TVSVVDKVVDKRGTRDVATAYLNYLYSTEGQRIAAQNYYRPRYANVAKEYAKEFPTLKLFTLAETFGDWTKVQKEHFASG
GSFDQISQR

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=330, Percent_Identity=80.6060606060606, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1788765, Length=307, Percent_Identity=47.2312703583062, Blast_Score=286, Evalue=1e-78,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36863; Mature: 36732

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKWRAGVLLMLLSGSVAAKDIQLLNVSYDPTREFYQQYNKAFSQYWQQKTGDHVTVRQS
CCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
HGGSGKQATSVINGIEADVVTLALESDVDAIAERGRIDKNWITRLPDNSAPYTSTIVFLV
CCCCCHHHHHHHHCCCHHEEEEEECCCHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEEEE
RKGNPKQIHDWSDLIKPGVSIITPNPKTSGGARWKYLAAWGYALHQNNNDQAKAQAFVKV
ECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCEEEHEEHHHHEECCCCHHHHHHHHHHH
LFKNVEVLDSGARGATNTFVERGQGDVLIAWENEALLAINELNSKDQFEIITPSESILAE
HHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCEEEECCCHHHHCC
PTVSVVDKVVDKRGTRDVATAYLNYLYSTEGQRIAAQNYYRPRYANVAKEYAKEFPTLKL
CHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEHHHCCCCCHHHHHHHHHHHHCCCEEE
FTLAETFGDWTKVQKEHFASGGSFDQISQR
EHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
>Mature Secondary Structure 
SKWRAGVLLMLLSGSVAAKDIQLLNVSYDPTREFYQQYNKAFSQYWQQKTGDHVTVRQS
CCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
HGGSGKQATSVINGIEADVVTLALESDVDAIAERGRIDKNWITRLPDNSAPYTSTIVFLV
CCCCCHHHHHHHHCCCHHEEEEEECCCHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEEEE
RKGNPKQIHDWSDLIKPGVSIITPNPKTSGGARWKYLAAWGYALHQNNNDQAKAQAFVKV
ECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCEEEHEEHHHHEECCCCHHHHHHHHHHH
LFKNVEVLDSGARGATNTFVERGQGDVLIAWENEALLAINELNSKDQFEIITPSESILAE
HHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCEEEECCCHHHHCC
PTVSVVDKVVDKRGTRDVATAYLNYLYSTEGQRIAAQNYYRPRYANVAKEYAKEFPTLKL
CHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEHHHCCCCCHHHHHHHHHHHHCCCEEE
FTLAETFGDWTKVQKEHFASGGSFDQISQR
EHHHHHHHHHHHHHHHHHCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]