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The map label for this gene is emrD

Identifier: 85060151

GI number: 85060151

Start: 3786527

End: 3787693

Strand: Reverse

Name: emrD

Synonym: SG2173

Alternate gene names: 85060151

Gene position: 3787693-3786527 (Counterclockwise)

Preceding gene: 85060155

Following gene: 85060149

Centisome position: 90.81

GC content: 62.13

Gene sequence:

>1167_bases
ATGATGAACCCGCGGGCGTTATCCGTGCTGATGCTATTAATTATCCTGGTGGCCGTTGGGCAGATGGCGCAGACCATTTA
TGTACCGGGCATTCCTCTTATCGCCCGCGATCTCATGGTGCGCGAAGGGGCGATACAGCGACTGATGGCCGCCTATCTCC
TGTGCTATGGCGGTTCGCAGCTTATTTACGGCCCGCTCTCCGATCGGATAGGGCGTCGGCCGGTCATCTTGAGCGGGATG
GCCATCTTCTGTCTGGGGACGATGCTGGCCATGCTGAGCCCATCGCTATCGCTGCTGACGCTTGCCTGCGGTATCCAGGG
CATCGGCACCGGCGTGGGTGGCGTGATGGCGCGGACGCTGCCGCGGGACATCTGCGATGGACGCTCGCTGCGGCAGGCTA
ACGGCCTGCTCAATATGGGCATCTTAATCAGTCCGCTGCTGGCGCCGCTGATAGGCGGCGTGCTTGCCGACTGGCTGGGT
TGGCGCGCGTGCTTTGCATTTTTGCTGCTGCTGAGCTGTAGCGTTAGTCTGCTGGTCTGGGGGCGTTTACCGGAGACTCG
TCCGATGCGCCCGGCAGGCAGCGGCCGCAGCCTTGGTTTTTGGCGTCTGTTAAGCGAAAAGGTGTTTAATCATTACCTGC
TGATGCTGGTCGGCGGACTGTCGGGGATCGTCGCGTTCGAGGCCAGCAGCGGCGTGCTATTGGGCAGCATGGGACTGACG
GGCCGGGAGGTCAGCCTGCTATTCATCCTGCCTCTGCCGGGTACGTTTCTGGGCGCCTGGTATGCGGGGCGCGCAATCAA
GCCCTTCAGCGCGCTGATGTGGCGGGCGGTAATGAGCTGCCTGCTGGCGGGGATATTGATGTGGCTGCCGGCCTGGTTCG
GCATCATGACGCTATGGACGTTGCTCTTGCCCGCCGGCCTGTTTTTTTTCGGCGCTGGCATGCTGTTTCCGCTGGCCACT
ACCGGCGCGATGGAGCCTTATCCCTACTTGGCCGGCACTGCGGGCGCGCTGGTCGGCGGCCTGCAAAATATTGGCTCGGG
CGCCGTTGCCGGCTTCTCTTCGCTGCAGCAGCAGCAGGGGCAGTTCAGCCTCGGGATGATAACCTTCGCCATGGGAATGC
TGATCCTGCTGTGCTGGCTGCCGCTGTCGCAAACCATGTATCGCTAA

Upstream 100 bases:

>100_bases
GCTTGGGTGTGGTATTTATGTCCGAAAATAAACCTCTATTCTGTATATCTCAATATTAACAAGCAGTACATAGAATTAAT
AAGCTATATAAATAATTTCT

Downstream 100 bases:

>100_bases
GTCGGAGATCTTCTAGGGGAGGCGCACGGCGGCGCCCCGGCTGACGTCAGAGGATGAAAGGATGCAGCGCTGCATCCTTG
CGATCGAGATAGTGGATCGA

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MMNPRALSVLMLLIILVAVGQMAQTIYVPGIPLIARDLMVREGAIQRLMAAYLLCYGGSQLIYGPLSDRIGRRPVILSGM
AIFCLGTMLAMLSPSLSLLTLACGIQGIGTGVGGVMARTLPRDICDGRSLRQANGLLNMGILISPLLAPLIGGVLADWLG
WRACFAFLLLLSCSVSLLVWGRLPETRPMRPAGSGRSLGFWRLLSEKVFNHYLLMLVGGLSGIVAFEASSGVLLGSMGLT
GREVSLLFILPLPGTFLGAWYAGRAIKPFSALMWRAVMSCLLAGILMWLPAWFGIMTLWTLLLPAGLFFFGAGMLFPLAT
TGAMEPYPYLAGTAGALVGGLQNIGSGAVAGFSSLQQQQGQFSLGMITFAMGMLILLCWLPLSQTMYR

Sequences:

>Translated_388_residues
MMNPRALSVLMLLIILVAVGQMAQTIYVPGIPLIARDLMVREGAIQRLMAAYLLCYGGSQLIYGPLSDRIGRRPVILSGM
AIFCLGTMLAMLSPSLSLLTLACGIQGIGTGVGGVMARTLPRDICDGRSLRQANGLLNMGILISPLLAPLIGGVLADWLG
WRACFAFLLLLSCSVSLLVWGRLPETRPMRPAGSGRSLGFWRLLSEKVFNHYLLMLVGGLSGIVAFEASSGVLLGSMGLT
GREVSLLFILPLPGTFLGAWYAGRAIKPFSALMWRAVMSCLLAGILMWLPAWFGIMTLWTLLLPAGLFFFGAGMLFPLAT
TGAMEPYPYLAGTAGALVGGLQNIGSGAVAGFSSLQQQQGQFSLGMITFAMGMLILLCWLPLSQTMYR
>Mature_388_residues
MMNPRALSVLMLLIILVAVGQMAQTIYVPGIPLIARDLMVREGAIQRLMAAYLLCYGGSQLIYGPLSDRIGRRPVILSGM
AIFCLGTMLAMLSPSLSLLTLACGIQGIGTGVGGVMARTLPRDICDGRSLRQANGLLNMGILISPLLAPLIGGVLADWLG
WRACFAFLLLLSCSVSLLVWGRLPETRPMRPAGSGRSLGFWRLLSEKVFNHYLLMLVGGLSGIVAFEASSGVLLGSMGLT
GREVSLLFILPLPGTFLGAWYAGRAIKPFSALMWRAVMSCLLAGILMWLPAWFGIMTLWTLLLPAGLFFFGAGMLFPLAT
TGAMEPYPYLAGTAGALVGGLQNIGSGAVAGFSSLQQQQGQFSLGMITFAMGMLILLCWLPLSQTMYR

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=368, Percent_Identity=62.2282608695652, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI48994889, Length=331, Percent_Identity=26.5861027190332, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788509, Length=357, Percent_Identity=25.7703081232493, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790146, Length=329, Percent_Identity=28.8753799392097, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790794, Length=325, Percent_Identity=26.4615384615385, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87081738, Length=155, Percent_Identity=29.6774193548387, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41434; Mature: 41434

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNPRALSVLMLLIILVAVGQMAQTIYVPGIPLIARDLMVREGAIQRLMAAYLLCYGGSQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LIYGPLSDRIGRRPVILSGMAIFCLGTMLAMLSPSLSLLTLACGIQGIGTGVGGVMARTL
EEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCHHHHHHHHH
PRDICDGRSLRQANGLLNMGILISPLLAPLIGGVLADWLGWRACFAFLLLLSCSVSLLVW
HHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRLPETRPMRPAGSGRSLGFWRLLSEKVFNHYLLMLVGGLSGIVAFEASSGVLLGSMGLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCC
GREVSLLFILPLPGTFLGAWYAGRAIKPFSALMWRAVMSCLLAGILMWLPAWFGIMTLWT
CCCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLPAGLFFFGAGMLFPLATTGAMEPYPYLAGTAGALVGGLQNIGSGAVAGFSSLQQQQG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHHHHHHHHHCCCHHHHHHHHHHHHCC
QFSLGMITFAMGMLILLCWLPLSQTMYR
CHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMNPRALSVLMLLIILVAVGQMAQTIYVPGIPLIARDLMVREGAIQRLMAAYLLCYGGSQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LIYGPLSDRIGRRPVILSGMAIFCLGTMLAMLSPSLSLLTLACGIQGIGTGVGGVMARTL
EEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCHHHHHHHHH
PRDICDGRSLRQANGLLNMGILISPLLAPLIGGVLADWLGWRACFAFLLLLSCSVSLLVW
HHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRLPETRPMRPAGSGRSLGFWRLLSEKVFNHYLLMLVGGLSGIVAFEASSGVLLGSMGLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCC
GREVSLLFILPLPGTFLGAWYAGRAIKPFSALMWRAVMSCLLAGILMWLPAWFGIMTLWT
CCCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLPAGLFFFGAGMLFPLATTGAMEPYPYLAGTAGALVGGLQNIGSGAVAGFSSLQQQQG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHHHHHHHHHCCCHHHHHHHHHHHHCC
QFSLGMITFAMGMLILLCWLPLSQTMYR
CHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]