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The map label for this gene is pfkA

Identifier: 85060156

GI number: 85060156

Start: 3791678

End: 3792640

Strand: Reverse

Name: pfkA

Synonym: SG2178

Alternate gene names: 85060156

Gene position: 3792640-3791678 (Counterclockwise)

Preceding gene: 85060157

Following gene: 85060155

Centisome position: 90.93

GC content: 54.0

Gene sequence:

>963_bases
ATGATTAAAAAAATCGGTGTACTGACAAGCGGCGGTGATTCACCAGGAATGAATGCGGCCATCCGGGGCGTGGTACGGGC
CGGGCTTTCCGAAGGCCTGGAGGTCTACGGCATTTATGACGGTTACCTGGGCCTGTTTCAGGATCGGATAGCGAAACTGG
ACCGTTATAGCGTTTCAGATATGATTAACCGCGGCGGCACCTTTCTTGGTTCTGCGCGTTTTCCGGAATTTCGAGAGGAA
GGCACCCGCGCTATCGCGATTGAGAACATGACCAAACGTGGTCTGGATGCGCTGGTGGTAATCGGTGGCGATGGTTCCTA
CATGGGCGCCAAGCGTTTGACCGAAATGGGTTTCCCCTGCATTGGCCTGCCGGGCACGATTGATAATGACGTGGCGGGCA
CCGATTACACCATTGGCTACTTCACCGCGCTGGAAACCGTCGTCGAGGCCATTGACCGGCTGCGCGATACATCTACGTCA
CATCAGCGTATTTCTATTGTTGAGGTCATGGGCCGGTATTGTGGCGATCTGACCATGGCGGCTGCTATTGCCGGCGGCTG
TGAGTTCATCGTGTTGCCTGAAGTGGAATTCAAACCGGAAGACCTGGTCTACGAAATCAAGGCGGGTATCGCTAAGGGTA
AAAAACACGCCATTGTCGCGATTACCGAATACATCTGTAATGTTGCTGAGCTTGCGCAATATATTGAAAAAGAGACGGGA
CGGGAAACCCGCGCCACCGTACTGGGTCATATCCAGCGCGGCGGTAGGCCGGTGGCTTACGACCGTATTTTGGCCTCGCT
CATGGGTGCCTATTCTATTGAACTGCTGTTGCAGGGCTACGGCGGCCGTTGCGTCGGGGTGCAAAACGAGCGACTGGTGC
ATCACGACATCGTCGACGCCATTGAAAATATGAAACGTCCTTTCCGCAGCAATATCCTGGAAACGGCAAAAAAACTCTTC
TGA

Upstream 100 bases:

>100_bases
TATACTCTTTCTTCACAAGCCCCGGCATTCGTTGCGGGTGGGAAAAGCGGTAACAAACGTATTGTATTCTACAAGCGGTA
TCTTAAGTTCAGAGGTAGTC

Downstream 100 bases:

>100_bases
TTTTGCCGCTTGTCTTGCCCTCGGATCTGAGGGCAAGTGCTCTAGCGCGCCTGACGCTCACCCCGCCATGCTATAAAGCA
AATTATATTCTTTGGTTATA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGLEVYGIYDGYLGLFQDRIAKLDRYSVSDMINRGGTFLGSARFPEFREE
GTRAIAIENMTKRGLDALVVIGGDGSYMGAKRLTEMGFPCIGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSTS
HQRISIVEVMGRYCGDLTMAAAIAGGCEFIVLPEVEFKPEDLVYEIKAGIAKGKKHAIVAITEYICNVAELAQYIEKETG
RETRATVLGHIQRGGRPVAYDRILASLMGAYSIELLLQGYGGRCVGVQNERLVHHDIVDAIENMKRPFRSNILETAKKLF

Sequences:

>Translated_320_residues
MIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGLEVYGIYDGYLGLFQDRIAKLDRYSVSDMINRGGTFLGSARFPEFREE
GTRAIAIENMTKRGLDALVVIGGDGSYMGAKRLTEMGFPCIGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSTS
HQRISIVEVMGRYCGDLTMAAAIAGGCEFIVLPEVEFKPEDLVYEIKAGIAKGKKHAIVAITEYICNVAELAQYIEKETG
RETRATVLGHIQRGGRPVAYDRILASLMGAYSIELLLQGYGGRCVGVQNERLVHHDIVDAIENMKRPFRSNILETAKKLF
>Mature_320_residues
MIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGLEVYGIYDGYLGLFQDRIAKLDRYSVSDMINRGGTFLGSARFPEFREE
GTRAIAIENMTKRGLDALVVIGGDGSYMGAKRLTEMGFPCIGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSTS
HQRISIVEVMGRYCGDLTMAAAIAGGCEFIVLPEVEFKPEDLVYEIKAGIAKGKKHAIVAITEYICNVAELAQYIEKETG
RETRATVLGHIQRGGRPVAYDRILASLMGAYSIELLLQGYGGRCVGVQNERLVHHDIVDAIENMKRPFRSNILETAKKLF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=39.871382636656, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI266453768, Length=360, Percent_Identity=36.9444444444444, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI266453748, Length=360, Percent_Identity=36.9444444444444, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4505749, Length=360, Percent_Identity=36.9444444444444, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI266453619, Length=360, Percent_Identity=36.9444444444444, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI48762920, Length=360, Percent_Identity=36.3888888888889, Blast_Score=195, Evalue=5e-50,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=83.75, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI25147584, Length=312, Percent_Identity=36.5384615384615, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=37.012987012987, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=29.6551724137931, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321679, Length=341, Percent_Identity=36.6568914956012, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6323861, Length=347, Percent_Identity=32.5648414985591, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI17647809, Length=312, Percent_Identity=39.7435897435897, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI28573326, Length=312, Percent_Identity=40.3846153846154, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=39.871382636656, Blast_Score=204, Evalue=7e-53,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_SODGM (Q2NQX2)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455858.1
- ProteinModelPortal:   Q2NQX2
- SMR:   Q2NQX2
- STRING:   Q2NQX2
- GeneID:   3867952
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2178
- NMPDR:   fig|343509.6.peg.4796
- eggNOG:   COG0205
- HOGENOM:   HBG405044
- OMA:   VDAIENM
- PhylomeDB:   Q2NQX2
- ProtClustDB:   PRK03202
- BioCyc:   SGLO343509:SG2178-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34860; Mature: 34860

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 128-128 BINDING 163-163 BINDING 244-244 BINDING 250-250 BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGLEVYGIYDGYLGLFQDRIAKLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFREEGTRAIAIENMTKRGLDALVVIGGDGSYMGAKRLTEMGFPC
HHHCCCCEECCCCCCHHHHCCCEEEEECCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
IGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSTSHQRISIVEVMGRYCGDLTMA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AAIAGGCEFIVLPEVEFKPEDLVYEIKAGIAKGKKHAIVAITEYICNVAELAQYIEKETG
HHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHCHHHHHHHHHHHCC
RETRATVLGHIQRGGRPVAYDRILASLMGAYSIELLLQGYGGRCVGVQNERLVHHDIVDA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
IENMKRPFRSNILETAKKLF
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKKIGVLTSGGDSPGMNAAIRGVVRAGLSEGLEVYGIYDGYLGLFQDRIAKLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFREEGTRAIAIENMTKRGLDALVVIGGDGSYMGAKRLTEMGFPC
HHHCCCCEECCCCCCHHHHCCCEEEEECCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
IGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSTSHQRISIVEVMGRYCGDLTMA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AAIAGGCEFIVLPEVEFKPEDLVYEIKAGIAKGKKHAIVAITEYICNVAELAQYIEKETG
HHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHCHHHHHHHHHHHCC
RETRATVLGHIQRGGRPVAYDRILASLMGAYSIELLLQGYGGRCVGVQNERLVHHDIVDA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
IENMKRPFRSNILETAKKLF
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA