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The map label for this gene is lexA

Identifier: 85060119

GI number: 85060119

Start: 3717642

End: 3718250

Strand: Reverse

Name: lexA

Synonym: SG2141

Alternate gene names: 85060119

Gene position: 3718250-3717642 (Counterclockwise)

Preceding gene: 85060121

Following gene: 85060117

Centisome position: 89.14

GC content: 56.32

Gene sequence:

>609_bases
ATGAAAGCGCTAACCACCAGACAAAAAGAGGTTTTTGACCTGATCCGCGACCATATCACGCAGACCGGCATGCCCCCCAC
GCGTGCCGAGATTGCGTCGCGTCTGGGGTTTCGTTCGCCCAATGCGGCGGAAGAGCATTTGAAAGCGCTGGCGCGCAAGG
GCGCCATTGAAATTGTCTCCGGCGCGTCGCGCGGCATTCGGCTGATGATAGAAGAAGAAAGCGGTTTGCCGCTGATTGGG
CGCGTGGCCGCCGGCGAGCCTTTGCTGGCAACGCAGCACATTGAAAGCCACTATCAGGTGGATCCCGCCCTGTTTAAGCC
CCATGCTGACTTCCTGCTGCGGGTAAGCGGCATGTCGATGAAGGATATCGGCATCATGGACGGTGATCTGCTGGCGGTAC
ATAAAACGCAGGATGCCTGCAATGGCCAGGTTGTTGTGGCGCGCATTGATGATGATGTTACCGTTAAGCGGTTGAAACGC
CAGGGTAACATTGTTGAACTATTACCGGAAAACAGTGAGTTTGACCCGATCGTGGTGGACCTGCGGCAGCAAGAGCTGAC
TATTGAAGGGCTGGCGGTTGGCGTGATACGCAACGGCAACTGGCTTTAA

Upstream 100 bases:

>100_bases
ATTATCGTGAATGACACCCTATTTCGCGCGTTTTCGGTTTTCAATTGGCAATGACCTGTATATACTCTCAGAAAAACTGT
ATAAACAAACAGGGGGCGGA

Downstream 100 bases:

>100_bases
TCCGAGCACTGCGGGGGGAGCGTAACGCTGTCCCCGCTTGCGTGTTGCCTTATATTCCCTTCCCTTCATCCTCCACGCCT
ACAGCCTTAATCCTTTTGAT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MKALTTRQKEVFDLIRDHITQTGMPPTRAEIASRLGFRSPNAAEEHLKALARKGAIEIVSGASRGIRLMIEEESGLPLIG
RVAAGEPLLATQHIESHYQVDPALFKPHADFLLRVSGMSMKDIGIMDGDLLAVHKTQDACNGQVVVARIDDDVTVKRLKR
QGNIVELLPENSEFDPIVVDLRQQELTIEGLAVGVIRNGNWL

Sequences:

>Translated_202_residues
MKALTTRQKEVFDLIRDHITQTGMPPTRAEIASRLGFRSPNAAEEHLKALARKGAIEIVSGASRGIRLMIEEESGLPLIG
RVAAGEPLLATQHIESHYQVDPALFKPHADFLLRVSGMSMKDIGIMDGDLLAVHKTQDACNGQVVVARIDDDVTVKRLKR
QGNIVELLPENSEFDPIVVDLRQQELTIEGLAVGVIRNGNWL
>Mature_202_residues
MKALTTRQKEVFDLIRDHITQTGMPPTRAEIASRLGFRSPNAAEEHLKALARKGAIEIVSGASRGIRLMIEEESGLPLIG
RVAAGEPLLATQHIESHYQVDPALFKPHADFLLRVSGMSMKDIGIMDGDLLAVHKTQDACNGQVVVARIDDDVTVKRLKR
QGNIVELLPENSEFDPIVVDLRQQELTIEGLAVGVIRNGNWL

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, recA interacts with lexA causing an autocat

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=202, Percent_Identity=89.1089108910891, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787431, Length=117, Percent_Identity=34.1880341880342, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_SODGM (Q2NR09)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455821.1
- ProteinModelPortal:   Q2NR09
- SMR:   Q2NR09
- STRING:   Q2NR09
- GeneID:   3867302
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2141
- NMPDR:   fig|343509.6.peg.4712
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- PhylomeDB:   Q2NR09
- ProtClustDB:   PRK00215
- BioCyc:   SGLO343509:SG2141-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22230; Mature: 22230

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: ACT_SITE 119-119 ACT_SITE 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALTTRQKEVFDLIRDHITQTGMPPTRAEIASRLGFRSPNAAEEHLKALARKGAIEIVS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEC
GASRGIRLMIEEESGLPLIGRVAAGEPLLATQHIESHYQVDPALFKPHADFLLRVSGMSM
CCCCCEEEEEECCCCCCEEEEECCCCCEEHHHHHHHHHCCCHHHCCCCCCEEEEECCCCC
KDIGIMDGDLLAVHKTQDACNGQVVVARIDDDVTVKRLKRQGNIVELLPENSEFDPIVVD
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCEEEECCCCCCCCCEEEE
LRQQELTIEGLAVGVIRNGNWL
ECCCEEEEEEEEEEEEECCCCC
>Mature Secondary Structure
MKALTTRQKEVFDLIRDHITQTGMPPTRAEIASRLGFRSPNAAEEHLKALARKGAIEIVS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEC
GASRGIRLMIEEESGLPLIGRVAAGEPLLATQHIESHYQVDPALFKPHADFLLRVSGMSM
CCCCCEEEEEECCCCCCEEEEECCCCCEEHHHHHHHHHCCCHHHCCCCCCEEEEECCCCC
KDIGIMDGDLLAVHKTQDACNGQVVVARIDDDVTVKRLKRQGNIVELLPENSEFDPIVVD
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCEEEECCCCCCCCCEEEE
LRQQELTIEGLAVGVIRNGNWL
ECCCEEEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA