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The map label for this gene is prfA

Identifier: 85059854

GI number: 85059854

Start: 3211719

End: 3212801

Strand: Reverse

Name: prfA

Synonym: SG1876

Alternate gene names: 85059854

Gene position: 3212801-3211719 (Counterclockwise)

Preceding gene: 85059855

Following gene: 85059853

Centisome position: 77.02

GC content: 61.5

Gene sequence:

>1083_bases
ATGAAGTCCTCGATAGTCGCCAAATTGGAGGCGTTGCAAGAGCGCCGCGAAGAAGTGGAAGTTCTGCTCGGCGATCCCGG
CGTCATATCCGATCAGATCCGTTTTCGCGCCCTGTCGAAGGAGTACGCGCAGCTGTCTGATGTTACCCGCTGTTTTCAGC
ATTGGTGGCAGGTACAAGAAGATATCCACACCGCGGAGCACCTGCTGCAGGATCCGGAAATGCGCGACATGGCGCAGGAG
GAGCTGCTGGCGTCCGGCGCCAGTCTGGAGCAGCTTGAGCAGCAATTGCAGGTGCTGCTGTTGCCGCGGGATCCCGATGA
CGAACGCGGCTGTTTTCTGGAAGTGCGTGCCGGCACCGGCGGAGATGAGGCGGCGCTGTTCGCAGGCGACCTGTTTCGCA
TGTACAGCCGTTACGCCGAGACCCGGCGCTGGAAGGTGGAAATCGTCAGCGCCAGCTACGGCGAGCACGGCGGCTTCAAA
GAGGTCATCGCCAAGGTGTCCCATGAGGGCGCCTACGGACTACTGAAATTTGAATCCGGCGGTCATCGCGTGCAGCGCGT
ACCGGAAACCGAATCCCAGGGCCGTATCCATACCTCTGCCTGCACCGTGGCGGTGATGCCGGAAATCCCGGAGGCGGAGC
TGCCGGAAATCAACGCCGGCGATCTACGCATCGACACCTTCCGCTCCTCCGGCGCGGGCGGTCAGCATGTCAATACCACT
GACTCGGCTATCCGCATTACCCATCTGCCCACCGGGCTGGTGGTGGAATGCCAGGATGAACGCTCCCAGCACAAGAATAA
AGCCAAGGCCCTGGCGGTATTGGGCGCGCGTCTGCGCGCCGCCGAAGTCCAGCGCCGGCAGCAGGAAGAGTCCTCCACGC
GCCGTAATCTCCTGGGCAGCGGCGATCGGTCCGATCGCATCCGCACCTACAATTTTCCCCAGGGGCGGGTGACCGATCAC
CGTATCGGCCTGACGCTTTACAGGCTGGATGAAGTTATCGAAGGCAAGCTCGACATGCTGATCCAACCTATCATGCAGGA
GTATCAGGCTGATCAGCTCGCCGCGTTGTCCGAGACGCCCTGA

Upstream 100 bases:

>100_bases
GCAGCAGGCCGCCCGCGAAGGCGACGCCGAGCGTCTACAAATTTTGCGCGACGGTCTCGGACTGGACACGCACTGATATA
CATAAGGTAAACCACCACGT

Downstream 100 bases:

>100_bases
TGACCTGGCAGCAATGGCTGTCCCAGGCCGTGTCCCGGCTGCGCGCGTCCCAAAGTCCCAGCGCCAGGAGCGATGCCGAA
ATTCTGCTGGCGCAGCAGAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKSSIVAKLEALQERREEVEVLLGDPGVISDQIRFRALSKEYAQLSDVTRCFQHWWQVQEDIHTAEHLLQDPEMRDMAQE
ELLASGASLEQLEQQLQVLLLPRDPDDERGCFLEVRAGTGGDEAALFAGDLFRMYSRYAETRRWKVEIVSASYGEHGGFK
EVIAKVSHEGAYGLLKFESGGHRVQRVPETESQGRIHTSACTVAVMPEIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGLVVECQDERSQHKNKAKALAVLGARLRAAEVQRRQQEESSTRRNLLGSGDRSDRIRTYNFPQGRVTDH
RIGLTLYRLDEVIEGKLDMLIQPIMQEYQADQLAALSETP

Sequences:

>Translated_360_residues
MKSSIVAKLEALQERREEVEVLLGDPGVISDQIRFRALSKEYAQLSDVTRCFQHWWQVQEDIHTAEHLLQDPEMRDMAQE
ELLASGASLEQLEQQLQVLLLPRDPDDERGCFLEVRAGTGGDEAALFAGDLFRMYSRYAETRRWKVEIVSASYGEHGGFK
EVIAKVSHEGAYGLLKFESGGHRVQRVPETESQGRIHTSACTVAVMPEIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGLVVECQDERSQHKNKAKALAVLGARLRAAEVQRRQQEESSTRRNLLGSGDRSDRIRTYNFPQGRVTDH
RIGLTLYRLDEVIEGKLDMLIQPIMQEYQADQLAALSETP
>Mature_360_residues
MKSSIVAKLEALQERREEVEVLLGDPGVISDQIRFRALSKEYAQLSDVTRCFQHWWQVQEDIHTAEHLLQDPEMRDMAQE
ELLASGASLEQLEQQLQVLLLPRDPDDERGCFLEVRAGTGGDEAALFAGDLFRMYSRYAETRRWKVEIVSASYGEHGGFK
EVIAKVSHEGAYGLLKFESGGHRVQRVPETESQGRIHTSACTVAVMPEIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGLVVECQDERSQHKNKAKALAVLGARLRAAEVQRRQQEESSTRRNLLGSGDRSDRIRTYNFPQGRVTDH
RIGLTLYRLDEVIEGKLDMLIQPIMQEYQADQLAALSETP

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=294, Percent_Identity=47.9591836734694, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI34577120, Length=334, Percent_Identity=38.3233532934132, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=48.7179487179487, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=82.122905027933, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=39.4117647058824, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=344, Percent_Identity=33.4302325581395, Blast_Score=181, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=325, Percent_Identity=43.0769230769231, Blast_Score=254, Evalue=1e-68,
Organism=Drosophila melanogaster, GI19921226, Length=327, Percent_Identity=40.0611620795107, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SODGM (Q2NRS4)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455556.1
- ProteinModelPortal:   Q2NRS4
- SMR:   Q2NRS4
- STRING:   Q2NRS4
- GeneID:   3866350
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1876
- NMPDR:   fig|343509.6.peg.4014
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q2NRS4
- ProtClustDB:   PRK00591
- BioCyc:   SGLO343509:SG1876-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40444; Mature: 40444

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSIVAKLEALQERREEVEVLLGDPGVISDQIRFRALSKEYAQLSDVTRCFQHWWQVQE
CCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIHTAEHLLQDPEMRDMAQEELLASGASLEQLEQQLQVLLLPRDPDDERGCFLEVRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYSRYAETRRWKVEIVSASYGEHGGFKEVIAKVSHEGAYGLLKFESG
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPEIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
CCHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
DSAIRITHLPTGLVVECQDERSQHKNKAKALAVLGARLRAAEVQRRQQEESSTRRNLLGS
CCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRIRTYNFPQGRVTDHRIGLTLYRLDEVIEGKLDMLIQPIMQEYQADQLAALSETP
CCCCCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCC
>Mature Secondary Structure
MKSSIVAKLEALQERREEVEVLLGDPGVISDQIRFRALSKEYAQLSDVTRCFQHWWQVQE
CCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIHTAEHLLQDPEMRDMAQEELLASGASLEQLEQQLQVLLLPRDPDDERGCFLEVRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYSRYAETRRWKVEIVSASYGEHGGFKEVIAKVSHEGAYGLLKFESG
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPEIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
CCHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
DSAIRITHLPTGLVVECQDERSQHKNKAKALAVLGARLRAAEVQRRQQEESSTRRNLLGS
CCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRIRTYNFPQGRVTDHRIGLTLYRLDEVIEGKLDMLIQPIMQEYQADQLAALSETP
CCCCCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA