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The map label for this gene is hemK [H]

Identifier: 85059853

GI number: 85059853

Start: 3210871

End: 3211719

Strand: Reverse

Name: hemK [H]

Synonym: SG1875

Alternate gene names: 85059853

Gene position: 3211719-3210871 (Counterclockwise)

Preceding gene: 85059854

Following gene: 85059852

Centisome position: 77.0

GC content: 65.72

Gene sequence:

>849_bases
ATGACCTGGCAGCAATGGCTGTCCCAGGCCGTGTCCCGGCTGCGCGCGTCCCAAAGTCCCAGCGCCAGGAGCGATGCCGA
AATTCTGCTGGCGCAGCAGACCGGCGTGGCGCGCACCCGTCTGCTGGCTTTCGGCGAGACCCCGCTGACGGATGCCCAGC
ATGCTACGCTTGAGGCGTTATTAGCGCGGCGGGAACGGGGCGAACCCGTAGCCTATTTGACCGGCGAGCGTGAGTTCTGG
TCTTTGCCGCTACGCGTGTCGGCCGACACGCTGATTCCGCGCCCCGACACCGAATGTCTGGTTCAGCGCGCTCTGGACTT
GCTGTCGCCCGGCCGCGCCGAGGTGCTGGATTTGGGTACCGGCAGCGGCGCCATCGCGCTGGCGCTGGCTTCGGAGCGGC
CCGAATGGCGGATAACCGGCATCGATCGCCTGCCCGGCGCCGTGGCGCTCGCCCGCGCTAACGCCGCGCGCTTGGGGCTG
AGAAATGTGCAGTTCCACGAAGGTGACTGGTTTAAACCATTGCAGGCGCAACGCTATCGTCTTATTGTGAGTAATCCGCC
TTATATTAAGGCGGACGATCCCCATCTCATGCAGGGCGACGTACGCTTCGAGCCACGTAGCGCACTGGTGGCGGGGGAGG
ATGGGCTCCAGGATCTGGCGGCAATCTGCCGCGGGGCGGGGGCCCATTTGGAACCTGGCGGCTGGCTGGTGTTAGAGCAC
GGCTGGTGCCAGGGGGCAGCAGTGCGCGGCCTGCTCGCGGATACCGGTTTTGGTCAGATAGCCACGCTGCGCGATTATGG
CGACAATGAGCGCGTCAGCCAGGGACAATGGTCGCCCGCGCAAGCGTGA

Upstream 100 bases:

>100_bases
GGCTGGATGAAGTTATCGAAGGCAAGCTCGACATGCTGATCCAACCTATCATGCAGGAGTATCAGGCTGATCAGCTCGCC
GCGTTGTCCGAGACGCCCTG

Downstream 100 bases:

>100_bases
CCGACGTCAAGGGGGAGGAAATGCGTGCGCGACGAGCGACAACGGGATGATCGAGGACCAGGAATGGTCGCACCTGCCAG
CAGTTTCACCGCGGTATGCC

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: M.StyLTHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTWQQWLSQAVSRLRASQSPSARSDAEILLAQQTGVARTRLLAFGETPLTDAQHATLEALLARRERGEPVAYLTGEREFW
SLPLRVSADTLIPRPDTECLVQRALDLLSPGRAEVLDLGTGSGAIALALASERPEWRITGIDRLPGAVALARANAARLGL
RNVQFHEGDWFKPLQAQRYRLIVSNPPYIKADDPHLMQGDVRFEPRSALVAGEDGLQDLAAICRGAGAHLEPGGWLVLEH
GWCQGAAVRGLLADTGFGQIATLRDYGDNERVSQGQWSPAQA

Sequences:

>Translated_282_residues
MTWQQWLSQAVSRLRASQSPSARSDAEILLAQQTGVARTRLLAFGETPLTDAQHATLEALLARRERGEPVAYLTGEREFW
SLPLRVSADTLIPRPDTECLVQRALDLLSPGRAEVLDLGTGSGAIALALASERPEWRITGIDRLPGAVALARANAARLGL
RNVQFHEGDWFKPLQAQRYRLIVSNPPYIKADDPHLMQGDVRFEPRSALVAGEDGLQDLAAICRGAGAHLEPGGWLVLEH
GWCQGAAVRGLLADTGFGQIATLRDYGDNERVSQGQWSPAQA
>Mature_281_residues
TWQQWLSQAVSRLRASQSPSARSDAEILLAQQTGVARTRLLAFGETPLTDAQHATLEALLARRERGEPVAYLTGEREFWS
LPLRVSADTLIPRPDTECLVQRALDLLSPGRAEVLDLGTGSGAIALALASERPEWRITGIDRLPGAVALARANAARLGLR
NVQFHEGDWFKPLQAQRYRLIVSNPPYIKADDPHLMQGDVRFEPRSALVAGEDGLQDLAAICRGAGAHLEPGGWLVLEHG
WCQGAAVRGLLADTGFGQIATLRDYGDNERVSQGQWSPAQA

Specific function: Methylates the translation termination release factor RF1 on 'Gln-235' and RF2 on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=238, Percent_Identity=34.0336134453782, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1787463, Length=277, Percent_Identity=57.0397111913357, Blast_Score=311, Evalue=3e-86,
Organism=Escherichia coli, GI87082085, Length=191, Percent_Identity=35.0785340314136, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24582226, Length=235, Percent_Identity=32.7659574468085, Blast_Score=111, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30710; Mature: 30579

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWQQWLSQAVSRLRASQSPSARSDAEILLAQQTGVARTRLLAFGETPLTDAQHATLEAL
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHH
LARRERGEPVAYLTGEREFWSLPLRVSADTLIPRPDTECLVQRALDLLSPGRAEVLDLGT
HHHHHCCCCEEEEECCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECC
GSGAIALALASERPEWRITGIDRLPGAVALARANAARLGLRNVQFHEGDWFKPLQAQRYR
CCCEEEEEEECCCCCEEEECCCCCCCCEEEHHCCHHHHCCCCCEECCCCCCCCCCCCEEE
LIVSNPPYIKADDPHLMQGDVRFEPRSALVAGEDGLQDLAAICRGAGAHLEPGGWLVLEH
EEECCCCEEECCCCCEEECCEEECCCCEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEEC
GWCQGAAVRGLLADTGFGQIATLRDYGDNERVSQGQWSPAQA
CCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TWQQWLSQAVSRLRASQSPSARSDAEILLAQQTGVARTRLLAFGETPLTDAQHATLEAL
CHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHH
LARRERGEPVAYLTGEREFWSLPLRVSADTLIPRPDTECLVQRALDLLSPGRAEVLDLGT
HHHHHCCCCEEEEECCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECC
GSGAIALALASERPEWRITGIDRLPGAVALARANAARLGLRNVQFHEGDWFKPLQAQRYR
CCCEEEEEEECCCCCEEEECCCCCCCCEEEHHCCHHHHCCCCCEECCCCCCCCCCCCEEE
LIVSNPPYIKADDPHLMQGDVRFEPRSALVAGEDGLQDLAAICRGAGAHLEPGGWLVLEH
EEECCCCEEECCCCCEEECCEEECCCCEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEEC
GWCQGAAVRGLLADTGFGQIATLRDYGDNERVSQGQWSPAQA
CCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 2544564 [H]