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The map label for this gene is pdxB

Identifier: 85059599

GI number: 85059599

Start: 2776922

End: 2778055

Strand: Reverse

Name: pdxB

Synonym: SG1621

Alternate gene names: 85059599

Gene position: 2778055-2776922 (Counterclockwise)

Preceding gene: 85059601

Following gene: 85059598

Centisome position: 66.6

GC content: 65.78

Gene sequence:

>1134_bases
GTGAAAATCCTGGTGGATGAGAATATGCCTTATGCCGAGGCGCTGTTTGGGCGTCTTGGCGATGTGCAGACTGTGGCGGG
CAGGGCGATTCCCGCGCCGGCGCTGGCGCTGGCCGACGCGCTGATGGTGCGTTCGGTGACCCGCGTCGACGGAGCTCTGC
TCGACGGCAGCCGGGTGAAATTCGTCGGTACCGCTACCGCCGGCACCGATCATGTGGATGAAGACTGGTTGGCGCAGGCG
GGGATCGGTTTTTCCGTGGCACCGGGCTGCAACGCGATTGCGGTAGTGGAGTATGTCTTTTCCGCGTTGCTGTGGCTGGC
GCAGCGGGACGGTTTCGCGCTGCGCGACAAGACCGTCGGCATTGTTGGCGTCGGGAATGTCGGCGGCCGTCTGCAACGGC
GGCTCAACGCCTTTGGCGTGCGTACCCTGCTGTGCGATCCGCCGCTGGCGGAGGCCGGCGCTCCCGGCGACTGGCAGCCG
TTGGAAACGCTGGTGGCTGAAGCGGATGTGCTGACGTTCCATACGCCCCTTACGCTCGCCGGCCGGCATGCCACCTGGCA
TCAGGTGGACGAGGCGTTGTTGGCGGCGCTGCCCGCCGGCCGCATTATCATCAATGCCTGCCGCGGAGCGGTGGTGGATA
ACGCCGCGCTGCTGCAGGCGCTGGAGGGCGGCAAGCCGCTGAGCGTTGTGCTGGACGTCTGGGAGCCGGAGCCCGCGCTG
TCGTTGCCGCTGCTGGCCCGCGTCGATATCGGCACCGCCCATATCGCCGGTTATACCCTGGAAGGCAAGGCGCGCGGCAC
CACGCAGGTTTTCGATGCCTACAGCGCTTATGTCGGCAGTGATGAGCGCGCGAGCCTGGCGGCGCTGTTGCCGCCGCCGG
CGGTGGAGCGTATCCGTCTGCGGGGCGCGATTGATGAAGAGGCGCTGCGTCTGTTGTCGCATCTGGTCTATGATGTGCGG
CGAGATGATATGCAGTTACGTCGCGTGGCCGGACTGCCGGGGGAATTCGATCGCTTGCGCAAAAATTACTATCAGCGCCG
GGAGTGGTCGTCGCTGTGCGTGGAAACCGACAATAGCATTGGCGCGGATGCGCTGAGGCAGCTGGGGTTCCAAGCTCGGC
CGTTCGCCGGCTGA

Upstream 100 bases:

>100_bases
CAAAATGAAGCGTGATAGGGTTGGGATTCTTACGCATTCGGTGGCAGAATATTCCCCCCCCCCTTTAACGTTTGATGGCA
CTGTGCTGCCGGAGCCTGAT

Downstream 100 bases:

>100_bases
GGCTGCACGAGGCCTCAGCCTGTCGTGCCTGACTGTAGTTGCTCTCTTGGCCGCAGGATGCGCCCGGGACCTTTTTCGCT
ATTTTGATTGACGGATGCCG

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKILVDENMPYAEALFGRLGDVQTVAGRAIPAPALALADALMVRSVTRVDGALLDGSRVKFVGTATAGTDHVDEDWLAQA
GIGFSVAPGCNAIAVVEYVFSALLWLAQRDGFALRDKTVGIVGVGNVGGRLQRRLNAFGVRTLLCDPPLAEAGAPGDWQP
LETLVAEADVLTFHTPLTLAGRHATWHQVDEALLAALPAGRIIINACRGAVVDNAALLQALEGGKPLSVVLDVWEPEPAL
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFDAYSAYVGSDERASLAALLPPPAVERIRLRGAIDEEALRLLSHLVYDVR
RDDMQLRRVAGLPGEFDRLRKNYYQRREWSSLCVETDNSIGADALRQLGFQARPFAG

Sequences:

>Translated_377_residues
MKILVDENMPYAEALFGRLGDVQTVAGRAIPAPALALADALMVRSVTRVDGALLDGSRVKFVGTATAGTDHVDEDWLAQA
GIGFSVAPGCNAIAVVEYVFSALLWLAQRDGFALRDKTVGIVGVGNVGGRLQRRLNAFGVRTLLCDPPLAEAGAPGDWQP
LETLVAEADVLTFHTPLTLAGRHATWHQVDEALLAALPAGRIIINACRGAVVDNAALLQALEGGKPLSVVLDVWEPEPAL
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFDAYSAYVGSDERASLAALLPPPAVERIRLRGAIDEEALRLLSHLVYDVR
RDDMQLRRVAGLPGEFDRLRKNYYQRREWSSLCVETDNSIGADALRQLGFQARPFAG
>Mature_377_residues
MKILVDENMPYAEALFGRLGDVQTVAGRAIPAPALALADALMVRSVTRVDGALLDGSRVKFVGTATAGTDHVDEDWLAQA
GIGFSVAPGCNAIAVVEYVFSALLWLAQRDGFALRDKTVGIVGVGNVGGRLQRRLNAFGVRTLLCDPPLAEAGAPGDWQP
LETLVAEADVLTFHTPLTLAGRHATWHQVDEALLAALPAGRIIINACRGAVVDNAALLQALEGGKPLSVVLDVWEPEPAL
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFDAYSAYVGSDERASLAALLPPPAVERIRLRGAIDEEALRLLSHLVYDVR
RDDMQLRRVAGLPGEFDRLRKNYYQRREWSSLCVETDNSIGADALRQLGFQARPFAG

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=251, Percent_Identity=28.6852589641434, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI6912396, Length=316, Percent_Identity=27.5316455696203, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4557497, Length=228, Percent_Identity=25.8771929824561, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI61743967, Length=228, Percent_Identity=25.8771929824561, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788660, Length=372, Percent_Identity=66.3978494623656, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1787645, Length=213, Percent_Identity=31.4553990610329, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082289, Length=248, Percent_Identity=28.2258064516129, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789279, Length=259, Percent_Identity=28.1853281853282, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=263, Percent_Identity=26.9961977186312, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320925, Length=322, Percent_Identity=28.2608695652174, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322116, Length=278, Percent_Identity=28.7769784172662, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=161, Percent_Identity=30.4347826086957, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921140, Length=299, Percent_Identity=27.7591973244147, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574286, Length=240, Percent_Identity=26.25, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24646446, Length=228, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24646448, Length=228, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24646452, Length=228, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24646450, Length=228, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_SODGM (Q2NSH9)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455301.1
- ProteinModelPortal:   Q2NSH9
- SMR:   Q2NSH9
- STRING:   Q2NSH9
- GeneID:   3867799
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1621
- NMPDR:   fig|343509.6.peg.3449
- eggNOG:   COG0111
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- PhylomeDB:   Q2NSH9
- BioCyc:   SGLO343509:SG1621-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 40429; Mature: 40429

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 208-208 ACT_SITE 237-237 ACT_SITE 254-254 BINDING 45-45 BINDING 66-66 BINDING 146-146 BINDING 175-175 BINDING 232-232 BINDING 257-257 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVDENMPYAEALFGRLGDVQTVAGRAIPAPALALADALMVRSVTRVDGALLDGSRVK
CEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCEEE
FVGTATAGTDHVDEDWLAQAGIGFSVAPGCNAIAVVEYVFSALLWLAQRDGFALRDKTVG
EEEECCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCEE
IVGVGNVGGRLQRRLNAFGVRTLLCDPPLAEAGAPGDWQPLETLVAEADVLTFHTPLTLA
EEEECCCCHHHHHHHHHHCCEEEECCCCHHHCCCCCCCHHHHHHHHHCCEEEEECCEEEC
GRHATWHQVDEALLAALPAGRIIINACRGAVVDNAALLQALEGGKPLSVVLDVWEPEPAL
CCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHCHHHHHHHHCCCCCEEEEEEECCCCCCC
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFDAYSAYVGSDERASLAALLPPPAVERIRL
CCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHH
RGAIDEEALRLLSHLVYDVRRDDMQLRRVAGLPGEFDRLRKNYYQRREWSSLCVETDNSI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCC
GADALRQLGFQARPFAG
CHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKILVDENMPYAEALFGRLGDVQTVAGRAIPAPALALADALMVRSVTRVDGALLDGSRVK
CEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCEEE
FVGTATAGTDHVDEDWLAQAGIGFSVAPGCNAIAVVEYVFSALLWLAQRDGFALRDKTVG
EEEECCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCEE
IVGVGNVGGRLQRRLNAFGVRTLLCDPPLAEAGAPGDWQPLETLVAEADVLTFHTPLTLA
EEEECCCCHHHHHHHHHHCCEEEECCCCHHHCCCCCCCHHHHHHHHHCCEEEEECCEEEC
GRHATWHQVDEALLAALPAGRIIINACRGAVVDNAALLQALEGGKPLSVVLDVWEPEPAL
CCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHCHHHHHHHHCCCCCEEEEEEECCCCCCC
SLPLLARVDIGTAHIAGYTLEGKARGTTQVFDAYSAYVGSDERASLAALLPPPAVERIRL
CCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHH
RGAIDEEALRLLSHLVYDVRRDDMQLRRVAGLPGEFDRLRKNYYQRREWSSLCVETDNSI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCC
GADALRQLGFQARPFAG
CHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA