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The map label for this gene is nuoB

Identifier: 85059578

GI number: 85059578

Start: 2747490

End: 2748164

Strand: Reverse

Name: nuoB

Synonym: SG1600

Alternate gene names: 85059578

Gene position: 2748164-2747490 (Counterclockwise)

Preceding gene: 85059579

Following gene: 85059577

Centisome position: 65.89

GC content: 58.96

Gene sequence:

>675_bases
ATGGATTACACCCTCACCCGCGCGGAGCCGGACGGAGATAACGAGCGGTATCCCCTGCAAAAACAGGAAATCGTGGCGGA
TCCGCTGGAGCAGCACGTCCATCGCAGCGTATACCTCGGCAAGCTTGAGAGAGCGCTGCACAATGCGGTGAACTGGGGAC
GCGGCAATTCGCTTTGGCCTTACAACTTCGGCCTCTCCTGCTGCTATGTCGAAATGACCACCTCCTTTACCGCTGTGCAC
GACGTGGCGCGCTTCGGTTCGGAAGTTATCCGCGCCTCACCGCGCCAGGCGGATTTCATGGTGGTGGCGGGGACGCCTTT
CACCAAGATGGCGCCCGTCATCCAGCGTCTTTACGACCAAATGCTGGAGCCGAAATGGGTTATCTCCATGGGCGCGTGTG
CCAATTCCGGCGGAATGTACGATATTTATTCTGTTGTGCAGGGCGTTGATAAATTCCTGCCGGTGGACGTCTACATTCCC
GGCTGTCCGCCGCGCCCTGAAGCGTACATCCAGGCGCTGCTGTTGCTGAAGGAGTCTATCGGCAAAGAACGCCGCCCGCT
CTCCTGGGTGGTGGGCGATCAGGGCGTCTATCGCGCCAACATGGAGTCGGAACGTCAACGTAAGCACGGTGAGCGTATCG
CGGTCACCCATCTGCGCACCTCCGACGAAATTTAA

Upstream 100 bases:

>100_bases
CGGTGCCCTTGACTGGACGCCGGAGCGCTCCCGACGTTTGCGCCGGGCAGGCCCCATTGGCGAAACCCCCCGTCATCAAG
AGTAACAGCGAGGCAGCAAA

Downstream 100 bases:

>100_bases
CGCGCGGATACGCATGCTGGTCGCGGCTGAATAAGTGGGGCGGCATGTGCCGTCACAGCCAAATGTGGTGAAGATAACTA
TGACAGAGTTGATGACGCAG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MDYTLTRAEPDGDNERYPLQKQEIVADPLEQHVHRSVYLGKLERALHNAVNWGRGNSLWPYNFGLSCCYVEMTTSFTAVH
DVARFGSEVIRASPRQADFMVVAGTPFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIP
GCPPRPEAYIQALLLLKESIGKERRPLSWVVGDQGVYRANMESERQRKHGERIAVTHLRTSDEI

Sequences:

>Translated_224_residues
MDYTLTRAEPDGDNERYPLQKQEIVADPLEQHVHRSVYLGKLERALHNAVNWGRGNSLWPYNFGLSCCYVEMTTSFTAVH
DVARFGSEVIRASPRQADFMVVAGTPFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIP
GCPPRPEAYIQALLLLKESIGKERRPLSWVVGDQGVYRANMESERQRKHGERIAVTHLRTSDEI
>Mature_224_residues
MDYTLTRAEPDGDNERYPLQKQEIVADPLEQHVHRSVYLGKLERALHNAVNWGRGNSLWPYNFGLSCCYVEMTTSFTAVH
DVARFGSEVIRASPRQADFMVVAGTPFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIP
GCPPRPEAYIQALLLLKESIGKERRPLSWVVGDQGVYRANMESERQRKHGERIAVTHLRTSDEI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=145, Percent_Identity=54.4827586206897, Blast_Score=165, Evalue=4e-41,
Organism=Escherichia coli, GI1788624, Length=224, Percent_Identity=84.375, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=41.8032786885246, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788834, Length=160, Percent_Identity=35, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=145, Percent_Identity=50.3448275862069, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=51.0344827586207, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=51.0344827586207, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24651058, Length=145, Percent_Identity=52.4137931034483, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_SODGM (Q2NSK0)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455280.1
- ProteinModelPortal:   Q2NSK0
- SMR:   Q2NSK0
- STRING:   Q2NSK0
- GeneID:   3867895
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1600
- NMPDR:   fig|343509.6.peg.3408
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   PRPEAYI
- PhylomeDB:   Q2NSK0
- ProtClustDB:   PRK06411
- BioCyc:   SGLO343509:SG1600-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 25393; Mature: 25393

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTLTRAEPDGDNERYPLQKQEIVADPLEQHVHRSVYLGKLERALHNAVNWGRGNSLWP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YNFGLSCCYVEMTTSFTAVHDVARFGSEVIRASPRQADFMVVAGTPFTKMAPVIQRLYDQ
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHH
MLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIPGCPPRPEAYIQALLLLKESI
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
GKERRPLSWVVGDQGVYRANMESERQRKHGERIAVTHLRTSDEI
CCCCCCCEEEECCCCEEECCCHHHHHHHCCCEEEEEEECCCCCC
>Mature Secondary Structure
MDYTLTRAEPDGDNERYPLQKQEIVADPLEQHVHRSVYLGKLERALHNAVNWGRGNSLWP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YNFGLSCCYVEMTTSFTAVHDVARFGSEVIRASPRQADFMVVAGTPFTKMAPVIQRLYDQ
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHH
MLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIPGCPPRPEAYIQALLLLKESI
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
GKERRPLSWVVGDQGVYRANMESERQRKHGERIAVTHLRTSDEI
CCCCCCCEEEECCCCEEECCCHHHHHHHCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA