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The map label for this gene is nuoM [H]

Identifier: 85059568

GI number: 85059568

Start: 2735007

End: 2736539

Strand: Reverse

Name: nuoM [H]

Synonym: SG1590

Alternate gene names: 85059568

Gene position: 2736539-2735007 (Counterclockwise)

Preceding gene: 85059569

Following gene: 85059567

Centisome position: 65.61

GC content: 58.19

Gene sequence:

>1533_bases
ATGCTACTACCTTGGCTAATTCTTATCCCCTTTATCGGCGGTCTGCTGTGTTGGCAATGTGAGCGTTTCGGTACCCGTGT
ACCGCGCTGGATAGCCTTGATTGCCATGGGGCTGACACTGGCGCTTTCATTGCTGCTGTGGCAGCAGGGAGGATACACCC
TGGCGATGGCGCAGGGGCTGCCGCAATGGCAGGTGGAGTATCTGCTCCCCTGGATCCCGCGCTTCGGTATTAGCATCTAT
CTGGCGCTGGACGGCCTGTCGCTGCTGATGGTAGTGCTGGCCGGGCTGCTCGGGGTGCTGGCTATCCTCTGTTCCTGGCG
TGAAATCCAGCGCTACCAGGGGTTCTTCCACCTCAATCTGCTGTGGATCCTCGGCGGCGTTATCGGTGTGTTCCTGGCTA
TCGACATGTTTCTCTTCTTTTTCTTCTGGGAAATGATGCTGGTGCCGATGTATTTCCTCATCGCGCTGTGGGGGCATAAG
GCGTCCGACGGTAAGACGCGTATCACCGCCGCAACTAAATTCTTCATCTATACCCAGGCGAGCGGTCTGGTAATGCTGGT
GGCCATCCTAGGTTTGGTGCTGGTGCACTATAACGCCACCGGCGTCTGGACCTTCAATTATGAAGACTTGCTGCGCACGC
CGATGTCGGAACGGATGGAATACCTGCTGATGCTGGGCTTCTTCTTGGCCTTCGCGGTTAAAATGCCGGTGGTGCCGCTG
CACGGCTGGCTGCCGGATGCCCATAGCCAGGCGCCTACCGCCGGTTCGGTGGACCTTGCGGGGATTTTGCTGAAAACCGC
GGCTTACGGGATGTTGCGTTTTAGCCTGCCGCTGTTCCCCCATGCGTCGCATCAGTTCGCGCACATCGCCATGTGGCTGG
GCGTGGTAGGCATTTTCTACGGCGCCTGGATGGCGTTTTCGCAAACCGATATCAAGCGCCTTATCGCCTATACCAGCGTG
TCCCATATGGGCTTTGTGCTGATCGCCATCTACAGCGGCAGCCAGCTGGCCTATCAGGGCGCGGTGGTACAGATGATCGC
CCACGGTTTGTCGGCCGCCGGCATGTTCATCATTTGCGGCCAGCTGTATGAGCGTTTGCACGCCCGCGATATGCGGCAGA
TGGGCGGTCTGTGGGGCCGGCTGAGCTTTATTCCGGCGTTGTCGCTGTTCTTCGCCGTTGCAACGCTCGGGATGCCGGGC
ACCGGCAACTTTGTTGGCGAGATTACCATTTTGTTCGGCAGTTTCCCGGTGGTACCTGTGATTACGGTGATTTCCACGTT
TGGTCTGGTGTTCGCGTCGGTCTACTCGCTTATCATGATGCAGCGCGCGTATTACGGCCCGGCCAAATCTACTGAGCCGC
TGCCGGGCATGACGCTGCGCGAACGGTTGATTATTTTGTTGCTGGTGGTGCTGGTGCTGCTGCTCGGGTTCTTCCCGCAG
CCGATTATGGATACTTCAGCCGCCGCCATGAGCAACATTCAGCACTGGTTCGCCGCCGACGCCGGTTCCACTATTTCAAC
GACAAGGCCGTAA

Upstream 100 bases:

>100_bases
ACCCCGAGCTTCGTTTCCGGCTTAGGGCGGAGCGGCGGGTAACGTACTGCTCCGCTGACACGCCAGTTTATTGATTTTAC
AATTAGGGACGCAAAACGCC

Downstream 100 bases:

>100_bases
TTCGCCATGACAATAACTCCTCAACAACTGATCGCATTATTACCGCTGTTGATCGTCGGATTGACGGTGGTGGTTGTGAT
GCTGTGCATTGCGTGGCGAC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13 [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MLLPWLILIPFIGGLLCWQCERFGTRVPRWIALIAMGLTLALSLLLWQQGGYTLAMAQGLPQWQVEYLLPWIPRFGISIY
LALDGLSLLMVVLAGLLGVLAILCSWREIQRYQGFFHLNLLWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLVAILGLVLVHYNATGVWTFNYEDLLRTPMSERMEYLLMLGFFLAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPHASHQFAHIAMWLGVVGIFYGAWMAFSQTDIKRLIAYTSV
SHMGFVLIAIYSGSQLAYQGAVVQMIAHGLSAAGMFIICGQLYERLHARDMRQMGGLWGRLSFIPALSLFFAVATLGMPG
TGNFVGEITILFGSFPVVPVITVISTFGLVFASVYSLIMMQRAYYGPAKSTEPLPGMTLRERLIILLLVVLVLLLGFFPQ
PIMDTSAAAMSNIQHWFAADAGSTISTTRP

Sequences:

>Translated_510_residues
MLLPWLILIPFIGGLLCWQCERFGTRVPRWIALIAMGLTLALSLLLWQQGGYTLAMAQGLPQWQVEYLLPWIPRFGISIY
LALDGLSLLMVVLAGLLGVLAILCSWREIQRYQGFFHLNLLWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLVAILGLVLVHYNATGVWTFNYEDLLRTPMSERMEYLLMLGFFLAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPHASHQFAHIAMWLGVVGIFYGAWMAFSQTDIKRLIAYTSV
SHMGFVLIAIYSGSQLAYQGAVVQMIAHGLSAAGMFIICGQLYERLHARDMRQMGGLWGRLSFIPALSLFFAVATLGMPG
TGNFVGEITILFGSFPVVPVITVISTFGLVFASVYSLIMMQRAYYGPAKSTEPLPGMTLRERLIILLLVVLVLLLGFFPQ
PIMDTSAAAMSNIQHWFAADAGSTISTTRP
>Mature_510_residues
MLLPWLILIPFIGGLLCWQCERFGTRVPRWIALIAMGLTLALSLLLWQQGGYTLAMAQGLPQWQVEYLLPWIPRFGISIY
LALDGLSLLMVVLAGLLGVLAILCSWREIQRYQGFFHLNLLWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLVAILGLVLVHYNATGVWTFNYEDLLRTPMSERMEYLLMLGFFLAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPHASHQFAHIAMWLGVVGIFYGAWMAFSQTDIKRLIAYTSV
SHMGFVLIAIYSGSQLAYQGAVVQMIAHGLSAAGMFIICGQLYERLHARDMRQMGGLWGRLSFIPALSLFFAVATLGMPG
TGNFVGEITILFGSFPVVPVITVISTFGLVFASVYSLIMMQRAYYGPAKSTEPLPGMTLRERLIILLLVVLVLLLGFFPQ
PIMDTSAAAMSNIQHWFAADAGSTISTTRP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=363, Percent_Identity=33.3333333333333, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1788613, Length=513, Percent_Identity=82.4561403508772, Blast_Score=845, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=349, Percent_Identity=25.7879656160458, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788831, Length=414, Percent_Identity=26.5700483091787, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788614, Length=413, Percent_Identity=27.3607748184019, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI145693160, Length=402, Percent_Identity=25.6218905472637, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788829, Length=318, Percent_Identity=26.1006289308176, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56588; Mature: 56588

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPWLILIPFIGGLLCWQCERFGTRVPRWIALIAMGLTLALSLLLWQQGGYTLAMAQGL
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
PQWQVEYLLPWIPRFGISIYLALDGLSLLMVVLAGLLGVLAILCSWREIQRYQGFFHLNL
CHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLVAILGLVLVHYNATGVWTFNYEDLLRTPMSERMEYLLMLGFFLAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPHASHQFAHIAMWLGVVGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GAWMAFSQTDIKRLIAYTSVSHMGFVLIAIYSGSQLAYQGAVVQMIAHGLSAAGMFIICG
HHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHARDMRQMGGLWGRLSFIPALSLFFAVATLGMPGTGNFVGEITILFGSFPVVPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEECCCCHHHH
ITVISTFGLVFASVYSLIMMQRAYYGPAKSTEPLPGMTLRERLIILLLVVLVLLLGFFPQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PIMDTSAAAMSNIQHWFAADAGSTISTTRP
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLLPWLILIPFIGGLLCWQCERFGTRVPRWIALIAMGLTLALSLLLWQQGGYTLAMAQGL
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
PQWQVEYLLPWIPRFGISIYLALDGLSLLMVVLAGLLGVLAILCSWREIQRYQGFFHLNL
CHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLVAILGLVLVHYNATGVWTFNYEDLLRTPMSERMEYLLMLGFFLAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPHASHQFAHIAMWLGVVGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GAWMAFSQTDIKRLIAYTSVSHMGFVLIAIYSGSQLAYQGAVVQMIAHGLSAAGMFIICG
HHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHARDMRQMGGLWGRLSFIPALSLFFAVATLGMPGTGNFVGEITILFGSFPVVPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEECCCCHHHH
ITVISTFGLVFASVYSLIMMQRAYYGPAKSTEPLPGMTLRERLIILLLVVLVLLLGFFPQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PIMDTSAAAMSNIQHWFAADAGSTISTTRP
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]