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The map label for this gene is trpB

Identifier: 85059376

GI number: 85059376

Start: 2321838

End: 2323028

Strand: Reverse

Name: trpB

Synonym: SG1398

Alternate gene names: 85059376

Gene position: 2323028-2321838 (Counterclockwise)

Preceding gene: 85059377

Following gene: 85059375

Centisome position: 55.69

GC content: 60.12

Gene sequence:

>1191_bases
ATGACACGACTTAATCCCTATTTTGGCGAGTTTGGCGGCATGTATGTGCCGCAAATCCTGATGCCTGCGCTGATTCAGCT
GGAAGACGCGTTCGTCAGCGCCCAGAGCGACCCGGCGTTTCAGGCCGAATTCAGCGATTTGCTGACAAACTACGCCGGCC
GACCGACCCCGCTCACGCTGTGCCGCAACCTGACCGCCGGCACCCGGACCAAACTTTATCTCAAACGCGAAGACCTGCTG
CACGGTGGCGCCCATAAAACCAACCAGGTGCTGGGGCAGGCGCTGCTGGCCAAACGGATGGGCAAAACTGAAATCATCGC
CGAAACCGGCGCCGGTCAGCACGGCGTGGCCTCCGCCCTGTCCTCCGCACTGCTGGGGCTGAAATGCCGTATTTATATGG
GCGCCAAAGACATCGAACGCCAGTCTCCCAATGTTTTTCGCATGCGGCTGATGGGCGCCGAGGTCATTCCGGTGCACAGC
GGCTCCTCCACCTTAAAAGACGCCTGCAACGAAGCGTTGCGCGACTGGTCCGATAGCTATGAGCGCGCCCACTACATGCT
TGGCACCGCCGCCGGCCCGCATCCGTTCCCGACCATCGTGCGCGAATTCCAGCGTATGATCGGCGAGGAAACGCGCGCGC
AGCTGCAAGAGCACGAGCAGCGCCTGCCGGATGCAGTCATCGCCTGCGTCGGCGGCGGCTCTAACGCCATCGGAATGTTC
GCCGATTTCATCGATGAACCGGCGGTTCGCCTGATAGGCGTAGAGCCGGGCGGCCTGGGTATCGAGACCAGGCAGCATGG
GGCGTCGTTGAAACACGGCCGCACCGGCATCTATTTCGGTATGAAGTCGCCGATGATGCAATCCGCCGAAGGGCAGATCG
AGGAATCCTACTCCATATCGGCCGGCCTGGATTTTCCCTCGGTGGGTCCCCAGCATGCCTACCTTGATAGCATAGGCAGG
GCGGAGTACATATCTGCTACCGACGAAGAAGCCCTGGATGCCTTCCGTCGCCTTTCCCGCCATGAGGGCATTATTCCGGC
GCTGGAATCGTCCCATGCGCTGGCCCACGCGCTGAGAATGATACAAGCGGAACCGGAGAAGGAGCAGATCCTGGTGGTCA
ACCTGTCCGGCCGTGGCGATAAAGATATTTTCACCGTTCATGATATTCTGAAGGCGCGAGGAGACATTTAA

Upstream 100 bases:

>100_bases
AAGCGCGCCGGGTATTAAAGATCATCATAAACTGGCTACGGTATTTCAGACGCTGAGCGCCTATTAACCTTCAGCCTGAC
ACCGTGAGAGATAATCAAGC

Downstream 100 bases:

>100_bases
TGGAACGCTATCAGCAATTATTCAGCCAGCTGGCGGCGCGCCATGAAGGCGCATTTGTGCCTTTTGTGACGCTTGGCGAT
CCCAACCCCACACTCTCGCT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTRLNPYFGEFGGMYVPQILMPALIQLEDAFVSAQSDPAFQAEFSDLLTNYAGRPTPLTLCRNLTAGTRTKLYLKREDLL
HGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALSSALLGLKCRIYMGAKDIERQSPNVFRMRLMGAEVIPVHS
GSSTLKDACNEALRDWSDSYERAHYMLGTAAGPHPFPTIVREFQRMIGEETRAQLQEHEQRLPDAVIACVGGGSNAIGMF
ADFIDEPAVRLIGVEPGGLGIETRQHGASLKHGRTGIYFGMKSPMMQSAEGQIEESYSISAGLDFPSVGPQHAYLDSIGR
AEYISATDEEALDAFRRLSRHEGIIPALESSHALAHALRMIQAEPEKEQILVVNLSGRGDKDIFTVHDILKARGDI

Sequences:

>Translated_396_residues
MTRLNPYFGEFGGMYVPQILMPALIQLEDAFVSAQSDPAFQAEFSDLLTNYAGRPTPLTLCRNLTAGTRTKLYLKREDLL
HGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALSSALLGLKCRIYMGAKDIERQSPNVFRMRLMGAEVIPVHS
GSSTLKDACNEALRDWSDSYERAHYMLGTAAGPHPFPTIVREFQRMIGEETRAQLQEHEQRLPDAVIACVGGGSNAIGMF
ADFIDEPAVRLIGVEPGGLGIETRQHGASLKHGRTGIYFGMKSPMMQSAEGQIEESYSISAGLDFPSVGPQHAYLDSIGR
AEYISATDEEALDAFRRLSRHEGIIPALESSHALAHALRMIQAEPEKEQILVVNLSGRGDKDIFTVHDILKARGDI
>Mature_395_residues
TRLNPYFGEFGGMYVPQILMPALIQLEDAFVSAQSDPAFQAEFSDLLTNYAGRPTPLTLCRNLTAGTRTKLYLKREDLLH
GGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALSSALLGLKCRIYMGAKDIERQSPNVFRMRLMGAEVIPVHSG
SSTLKDACNEALRDWSDSYERAHYMLGTAAGPHPFPTIVREFQRMIGEETRAQLQEHEQRLPDAVIACVGGGSNAIGMFA
DFIDEPAVRLIGVEPGGLGIETRQHGASLKHGRTGIYFGMKSPMMQSAEGQIEESYSISAGLDFPSVGPQHAYLDSIGRA
EYISATDEEALDAFRRLSRHEGIIPALESSHALAHALRMIQAEPEKEQILVVNLSGRGDKDIFTVHDILKARGDI

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=395, Percent_Identity=85.5696202531646, Blast_Score=706, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=389, Percent_Identity=50.3856041131105, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_SODGM (Q2NT52)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455078.1
- HSSP:   P0A2K1
- ProteinModelPortal:   Q2NT52
- SMR:   Q2NT52
- STRING:   Q2NT52
- GeneID:   3867038
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1398
- NMPDR:   fig|343509.6.peg.2852
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- PhylomeDB:   Q2NT52
- ProtClustDB:   PRK04346
- BioCyc:   SGLO343509:SG1398-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43188; Mature: 43057

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLNPYFGEFGGMYVPQILMPALIQLEDAFVSAQSDPAFQAEFSDLLTNYAGRPTPLTL
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHH
CRNLTAGTRTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASAL
HHCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
SSALLGLKCRIYMGAKDIERQSPNVFRMRLMGAEVIPVHSGSSTLKDACNEALRDWSDSY
HHHHHHHEEEEEECCHHHHCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHH
ERAHYMLGTAAGPHPFPTIVREFQRMIGEETRAQLQEHEQRLPDAVIACVGGGSNAIGMF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHH
ADFIDEPAVRLIGVEPGGLGIETRQHGASLKHGRTGIYFGMKSPMMQSAEGQIEESYSIS
HHHHCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCEEEECCCCCHHHHHCCCCHHCCEEE
AGLDFPSVGPQHAYLDSIGRAEYISATDEEALDAFRRLSRHEGIIPALESSHALAHALRM
CCCCCCCCCCCHHHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IQAEPEKEQILVVNLSGRGDKDIFTVHDILKARGDI
HHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRLNPYFGEFGGMYVPQILMPALIQLEDAFVSAQSDPAFQAEFSDLLTNYAGRPTPLTL
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHH
CRNLTAGTRTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASAL
HHCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
SSALLGLKCRIYMGAKDIERQSPNVFRMRLMGAEVIPVHSGSSTLKDACNEALRDWSDSY
HHHHHHHEEEEEECCHHHHCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHH
ERAHYMLGTAAGPHPFPTIVREFQRMIGEETRAQLQEHEQRLPDAVIACVGGGSNAIGMF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHH
ADFIDEPAVRLIGVEPGGLGIETRQHGASLKHGRTGIYFGMKSPMMQSAEGQIEESYSIS
HHHHCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCEEEECCCCCHHHHHCCCCHHCCEEE
AGLDFPSVGPQHAYLDSIGRAEYISATDEEALDAFRRLSRHEGIIPALESSHALAHALRM
CCCCCCCCCCCHHHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IQAEPEKEQILVVNLSGRGDKDIFTVHDILKARGDI
HHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA