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The map label for this gene is trpA

Identifier: 85059375

GI number: 85059375

Start: 2321032

End: 2321838

Strand: Reverse

Name: trpA

Synonym: SG1397

Alternate gene names: 85059375

Gene position: 2321838-2321032 (Counterclockwise)

Preceding gene: 85059376

Following gene: 85059374

Centisome position: 55.66

GC content: 59.11

Gene sequence:

>807_bases
ATGGAACGCTATCAGCAATTATTCAGCCAGCTGGCGGCGCGCCATGAAGGCGCATTTGTGCCTTTTGTGACGCTTGGCGA
TCCCAACCCCACACTCTCGCTACGCATCATCGATACACTCATTGACGCCGGCGCCGATGCACTGGAGCTGGGCATCCCGT
TCTCCGACCCGCTGGCCGACGGCCCCACCATCCAGAACGCCAATCTGCGCGCGTTTTCCTCCGGCGTTACGCCGGCGCAC
TGTTTTGAAATGCTGGCAACAATTCGCCAAAAGTATCCCGCCATACCGATTGGTCTGTTGATGTATGCCAATCTGGTCTA
CAACGAGGGGATAGATGCCTTTTACGCCCACTGCGCCGAGGTCGGCATCGACTCGGTGCTGGTGGCGGATGTGCCGCTGC
AAGAAAGTCTGCCATTTCGCCAGGCCGCCCTGCGCCATAACGTGGCCCCTATTGTCATCTGCCCGCCCAACTCTGATGAT
GCTTTGCTGCGCGAAATCGCCTCGCACGGACGGGGATATACCTATCTGCTCGCGCGCGCCGGCGTTACCGGCAGCGAGAA
ACGCGCAAACACCCCGCTCAAGCATCTAATCGATACCCTGAAGGAATACCATGCCGCACCGACATTACAGGGATTCGGCA
TCTCCGAACCCGGTCAGGTCCGTACCGCGCTACATGCCGGCACGGCCGGCGCTATTTCCGGCTCGGCGATTGTGCGTATT
ATCGAAAAGCATGAGGATAATCCGGCCGTGATGTTGGAACAGCTGGCCCAATTTGTGCGTGAACTCAAGGCAGCGACCCG
CGGCTGA

Upstream 100 bases:

>100_bases
AACCGGAGAAGGAGCAGATCCTGGTGGTCAACCTGTCCGGCCGTGGCGATAAAGATATTTTCACCGTTCATGATATTCTG
AAGGCGCGAGGAGACATTTA

Downstream 100 bases:

>100_bases
CTGGCGCAAACGCAATTTATCATTAACACTTTCAATGCCGGGCATACACAGGGCCCCGGCAAAGAAACCAAGCATAAAAC
GACAACGCATTCCATCAAGG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MERYQQLFSQLAARHEGAFVPFVTLGDPNPTLSLRIIDTLIDAGADALELGIPFSDPLADGPTIQNANLRAFSSGVTPAH
CFEMLATIRQKYPAIPIGLLMYANLVYNEGIDAFYAHCAEVGIDSVLVADVPLQESLPFRQAALRHNVAPIVICPPNSDD
ALLREIASHGRGYTYLLARAGVTGSEKRANTPLKHLIDTLKEYHAAPTLQGFGISEPGQVRTALHAGTAGAISGSAIVRI
IEKHEDNPAVMLEQLAQFVRELKAATRG

Sequences:

>Translated_268_residues
MERYQQLFSQLAARHEGAFVPFVTLGDPNPTLSLRIIDTLIDAGADALELGIPFSDPLADGPTIQNANLRAFSSGVTPAH
CFEMLATIRQKYPAIPIGLLMYANLVYNEGIDAFYAHCAEVGIDSVLVADVPLQESLPFRQAALRHNVAPIVICPPNSDD
ALLREIASHGRGYTYLLARAGVTGSEKRANTPLKHLIDTLKEYHAAPTLQGFGISEPGQVRTALHAGTAGAISGSAIVRI
IEKHEDNPAVMLEQLAQFVRELKAATRG
>Mature_268_residues
MERYQQLFSQLAARHEGAFVPFVTLGDPNPTLSLRIIDTLIDAGADALELGIPFSDPLADGPTIQNANLRAFSSGVTPAH
CFEMLATIRQKYPAIPIGLLMYANLVYNEGIDAFYAHCAEVGIDSVLVADVPLQESLPFRQAALRHNVAPIVICPPNSDD
ALLREIASHGRGYTYLLARAGVTGSEKRANTPLKHLIDTLKEYHAAPTLQGFGISEPGQVRTALHAGTAGAISGSAIVRI
IEKHEDNPAVMLEQLAQFVRELKAATRG

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=75.2808988764045, Blast_Score=400, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321412, Length=240, Percent_Identity=30.8333333333333, Blast_Score=126, Evalue=4e-30,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_SODGM (Q2NT53)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455077.1
- HSSP:   P00929
- ProteinModelPortal:   Q2NT53
- SMR:   Q2NT53
- STRING:   Q2NT53
- GeneID:   3867037
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1397
- NMPDR:   fig|343509.6.peg.2851
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q2NT53
- ProtClustDB:   PRK13111
- BioCyc:   SGLO343509:SG1397-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28872; Mature: 28872

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERYQQLFSQLAARHEGAFVPFVTLGDPNPTLSLRIIDTLIDAGADALELGIPFSDPLAD
CCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTIQNANLRAFSSGVTPAHCFEMLATIRQKYPAIPIGLLMYANLVYNEGIDAFYAHCAE
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
VGIDSVLVADVPLQESLPFRQAALRHNVAPIVICPPNSDDALLREIASHGRGYTYLLARA
HCCCCEEEECCCHHHCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEC
GVTGSEKRANTPLKHLIDTLKEYHAAPTLQGFGISEPGQVRTALHAGTAGAISGSAIVRI
CCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
IEKHEDNPAVMLEQLAQFVRELKAATRG
HHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERYQQLFSQLAARHEGAFVPFVTLGDPNPTLSLRIIDTLIDAGADALELGIPFSDPLAD
CCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTIQNANLRAFSSGVTPAHCFEMLATIRQKYPAIPIGLLMYANLVYNEGIDAFYAHCAE
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
VGIDSVLVADVPLQESLPFRQAALRHNVAPIVICPPNSDDALLREIASHGRGYTYLLARA
HCCCCEEEECCCHHHCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEC
GVTGSEKRANTPLKHLIDTLKEYHAAPTLQGFGISEPGQVRTALHAGTAGAISGSAIVRI
CCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
IEKHEDNPAVMLEQLAQFVRELKAATRG
HHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA