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The map label for this gene is manX [H]

Identifier: 85059305

GI number: 85059305

Start: 2205460

End: 2206431

Strand: Reverse

Name: manX [H]

Synonym: SG1327

Alternate gene names: 85059305

Gene position: 2206431-2205460 (Counterclockwise)

Preceding gene: 85059306

Following gene: 85059304

Centisome position: 52.9

GC content: 53.81

Gene sequence:

>972_bases
GTGAGTATAGCTATTGTTATTGGCACTCACGGGTCAGCGGCCGAGCAATTACTGAAGACGGCTGAAATGATTTTAGGCGC
ACAAGATAACGTCGCCTGGATCGATTTCGTCCCAGGCGAAAACGCTGAAACATTGATTAAAAAATATACCGCCCGTCTAG
CGGAACTTGATACCACAACAGGCGTGTTATTTCTGGTCGATACCTGGGGAGGCAGCCCGTTTAATGCCGGCAGCCGGATT
GTCATCGATAAAGAAAATTATGAAGTCGTCACCGGCGTCAATATCCCCATGCTGGCCGAAACCTTCATGGCGCGCGACGA
TAATCCCGCTTTCCAGGAGCTGGTCGGGATAGCGGTGGAAACCGGACGTATCGGCGTGAAGGCGCTGAAAACCCCGGAAC
CGGAACAGGCCGCAGCTGCCGCAGCGCCGGTGGCTGCCCCGGTCGCGAAAGCCGCACCGATGGCTAACGGTCAGGGCGGC
CATATGACTATCGGTTTGGCCAGGATCGACGACCGTCTGATCCATGGCCAGGTGGCTACTCGCTGGACGAAAGAAACCAA
CGTTAAACGCATCATTGTGGTCAGCGACGAAGTGGCCACGGACACGGTGCGTAAAACCCTGCTCACCCAGGTAGCACCGC
CGGGCATCACGGCTCATGTGGTTGACGTGGCGAAAGCAATCCGTGTTTACGACAATCCGAAATATGCCAACGATCGCGTC
ATGTTGTTATTCACCAACCCCACCGATGTGGTGCGTCTGGTCGAAGGCGGTGTCGCAATTACTTCAGTCAATATCGGCGG
TATGGCCTTCCGCCAGGGCAAAACCCAGGTCAACAATGCCGTATCGGTAGACGAAAAAGATATCGAGGCATTCAACAAAC
TCAACGATCGCGGGATTGAACTGGAAGTGCGTAAAGTGTCCAGCGATTCCCGGTTGAAGATGATGGAGTTAATTAAAAAA
CTCGGCAGTTAA

Upstream 100 bases:

>100_bases
TCGTTCCTGACATTGCAGTTATACCACCAAAGAGTTAGGGCCCTCGACACACCCCTTGTGCGGAGCACATAACCCATTTT
GTCAATACAGGAGGTATCCA

Downstream 100 bases:

>100_bases
GCCCTACGCCGCATTATCGCCCTTAAGAGCGTAAATTTGCCTGGAAATGGATTCATCGCTCTATTACGCAAGGTAAGAGG
AGAAGTACAATGGAGATCAC

Product: PTS system mannose-specific IIAB component ManX

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSIAIVIGTHGSAAEQLLKTAEMILGAQDNVAWIDFVPGENAETLIKKYTARLAELDTTTGVLFLVDTWGGSPFNAGSRI
VIDKENYEVVTGVNIPMLAETFMARDDNPAFQELVGIAVETGRIGVKALKTPEPEQAAAAAAPVAAPVAKAAPMANGQGG
HMTIGLARIDDRLIHGQVATRWTKETNVKRIIVVSDEVATDTVRKTLLTQVAPPGITAHVVDVAKAIRVYDNPKYANDRV
MLLFTNPTDVVRLVEGGVAITSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFNKLNDRGIELEVRKVSSDSRLKMMELIKK
LGS

Sequences:

>Translated_323_residues
MSIAIVIGTHGSAAEQLLKTAEMILGAQDNVAWIDFVPGENAETLIKKYTARLAELDTTTGVLFLVDTWGGSPFNAGSRI
VIDKENYEVVTGVNIPMLAETFMARDDNPAFQELVGIAVETGRIGVKALKTPEPEQAAAAAAPVAAPVAKAAPMANGQGG
HMTIGLARIDDRLIHGQVATRWTKETNVKRIIVVSDEVATDTVRKTLLTQVAPPGITAHVVDVAKAIRVYDNPKYANDRV
MLLFTNPTDVVRLVEGGVAITSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFNKLNDRGIELEVRKVSSDSRLKMMELIKK
LGS
>Mature_322_residues
SIAIVIGTHGSAAEQLLKTAEMILGAQDNVAWIDFVPGENAETLIKKYTARLAELDTTTGVLFLVDTWGGSPFNAGSRIV
IDKENYEVVTGVNIPMLAETFMARDDNPAFQELVGIAVETGRIGVKALKTPEPEQAAAAAAPVAAPVAKAAPMANGQGGH
MTIGLARIDDRLIHGQVATRWTKETNVKRIIVVSDEVATDTVRKTLLTQVAPPGITAHVVDVAKAIRVYDNPKYANDRVM
LLFTNPTDVVRLVEGGVAITSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFNKLNDRGIELEVRKVSSDSRLKMMELIKKL
GS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=321, Percent_Identity=79.4392523364486, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI87082217, Length=158, Percent_Identity=37.9746835443038, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789527, Length=156, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 34670; Mature: 34539

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAIVIGTHGSAAEQLLKTAEMILGAQDNVAWIDFVPGENAETLIKKYTARLAELDTTT
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
GVLFLVDTWGGSPFNAGSRIVIDKENYEVVTGVNIPMLAETFMARDDNPAFQELVGIAVE
CEEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHEEE
TGRIGVKALKTPEPEQAAAAAAPVAAPVAKAAPMANGQGGHMTIGLARIDDRLIHGQVAT
CCCCCEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHCCHHHH
RWTKETNVKRIIVVSDEVATDTVRKTLLTQVAPPGITAHVVDVAKAIRVYDNPKYANDRV
EEECCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEE
MLLFTNPTDVVRLVEGGVAITSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFNKLNDRGIE
EEEEECCHHHHHHHCCCEEEEEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCCE
LEVRKVSSDSRLKMMELIKKLGS
EEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SIAIVIGTHGSAAEQLLKTAEMILGAQDNVAWIDFVPGENAETLIKKYTARLAELDTTT
EEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
GVLFLVDTWGGSPFNAGSRIVIDKENYEVVTGVNIPMLAETFMARDDNPAFQELVGIAVE
CEEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHEEE
TGRIGVKALKTPEPEQAAAAAAPVAAPVAKAAPMANGQGGHMTIGLARIDDRLIHGQVAT
CCCCCEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHCCHHHH
RWTKETNVKRIIVVSDEVATDTVRKTLLTQVAPPGITAHVVDVAKAIRVYDNPKYANDRV
EEECCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEE
MLLFTNPTDVVRLVEGGVAITSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFNKLNDRGIE
EEEEECCHHHHHHHCCCEEEEEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCCE
LEVRKVSSDSRLKMMELIKKLGS
EEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]