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The map label for this gene is manY [H]

Identifier: 85059304

GI number: 85059304

Start: 2204570

End: 2205370

Strand: Reverse

Name: manY [H]

Synonym: SG1326

Alternate gene names: 85059304

Gene position: 2205370-2204570 (Counterclockwise)

Preceding gene: 85059305

Following gene: 85059303

Centisome position: 52.87

GC content: 57.55

Gene sequence:

>801_bases
ATGGAGATCACCACGCTTCAAATTGTACTGATATTTGTCGTCGCCTGTATCGCAGGCATGGGATCGATTCTCGACGAATT
TCAGTTCCACCGCCCGCTGGTAGCCTGTACCTTAATTGGTCTGGTCCTGGACGACATGAAAACCGGTATCATCATCGGCG
GGACGTTGGAAATGATCGCGCTGGGCTGGATGAATATCGGCGCCGCGGTGGCACCCGATGCCGCTCTGGCGTCCATCATC
TCCACCATTCTGGTCATAGCCGGTAATCAAAGCGTCGGCGCCGGTATCGCTCTGGCGATTCCGCTGGCCGCCACCGGACA
GGTGCTGACCATCATCGTACGTACCATCACCGTCGCCTTCCAGCATGCCGCCGACAGCGCCGCCGAACGCGGCAACATGA
CGGCGCTGAGCTGGATCCATGTCTCGGCGCTGGTGCTGCAAGCTATGCGTATCGCCATTCCGGCGGTCATCGTGGGCGTG
TCGGTCGGTACCGACGCGGTGCACAATATGCTCAGCTCGATTCCAGAAATCGTCACCAACGGTCTGAATATCGCCGGTGG
CATGATTATGGTCGTCGGTTATGCGATGGTCATCAATATGATGCGCACAGGCTATTTGATGCCGTTCTTTTACCTCGGTT
TCGTCACCGCGGCTTTCACCAGCTTCAACCTGGTGGCGCTGGGGGTCATCGGCGTGGTCATGGCTATACTCTACATCCAG
CTCAGCCCACGCTATAACCGGGCCGCAGGACAGGCCGCACCGGCGCAGGGTGCTAACGATCTCGATAATGAACTAGATTA
A

Upstream 100 bases:

>100_bases
TCGGCAGTTAAGCCCTACGCCGCATTATCGCCCTTAAGAGCGTAAATTTGCCTGGAAATGGATTCATCGCTCTATTACGC
AAGGTAAGAGGAGAAGTACA

Downstream 100 bases:

>100_bases
TAGGTGAGAGACATGGTCGATACTACAGCTACAGGTCAAAAGAAACTGACGCCCGGTGATGTACGCGGCGTATTTATCCG
CTCCAACCTTTTCCAGGGAT

Product: PTS system mannose-specific IIC component ManY

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-P-Man; EIIC-Man; PTS system mannose-specific EIIC component [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MEITTLQIVLIFVVACIAGMGSILDEFQFHRPLVACTLIGLVLDDMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASII
STILVIAGNQSVGAGIALAIPLAATGQVLTIIVRTITVAFQHAADSAAERGNMTALSWIHVSALVLQAMRIAIPAVIVGV
SVGTDAVHNMLSSIPEIVTNGLNIAGGMIMVVGYAMVINMMRTGYLMPFFYLGFVTAAFTSFNLVALGVIGVVMAILYIQ
LSPRYNRAAGQAAPAQGANDLDNELD

Sequences:

>Translated_266_residues
MEITTLQIVLIFVVACIAGMGSILDEFQFHRPLVACTLIGLVLDDMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASII
STILVIAGNQSVGAGIALAIPLAATGQVLTIIVRTITVAFQHAADSAAERGNMTALSWIHVSALVLQAMRIAIPAVIVGV
SVGTDAVHNMLSSIPEIVTNGLNIAGGMIMVVGYAMVINMMRTGYLMPFFYLGFVTAAFTSFNLVALGVIGVVMAILYIQ
LSPRYNRAAGQAAPAQGANDLDNELD
>Mature_266_residues
MEITTLQIVLIFVVACIAGMGSILDEFQFHRPLVACTLIGLVLDDMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASII
STILVIAGNQSVGAGIALAIPLAATGQVLTIIVRTITVAFQHAADSAAERGNMTALSWIHVSALVLQAMRIAIPAVIVGV
SVGTDAVHNMLSSIPEIVTNGLNIAGGMIMVVGYAMVINMMRTGYLMPFFYLGFVTAAFTSFNLVALGVIGVVMAILYIQ
LSPRYNRAAGQAAPAQGANDLDNELD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3715

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788121, Length=266, Percent_Identity=86.0902255639098, Blast_Score=423, Evalue=1e-120,
Organism=Escherichia coli, GI1789528, Length=263, Percent_Identity=23.1939163498099, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004700
- InterPro:   IPR018404 [H]

Pfam domain/function: PF03609 EII-Sor [H]

EC number: NA

Molecular weight: Translated: 27685; Mature: 27685

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS51106 PTS_EIIC_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITTLQIVLIFVVACIAGMGSILDEFQFHRPLVACTLIGLVLDDMKTGIIIGGTLEMIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHH
LGWMNIGAAVAPDAALASIISTILVIAGNQSVGAGIALAIPLAATGQVLTIIVRTITVAF
HHHHHCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHCCCHHHHHHHHHHHHHH
QHAADSAAERGNMTALSWIHVSALVLQAMRIAIPAVIVGVSVGTDAVHNMLSSIPEIVTN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GLNIAGGMIMVVGYAMVINMMRTGYLMPFFYLGFVTAAFTSFNLVALGVIGVVMAILYIQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSPRYNRAAGQAAPAQGANDLDNELD
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEITTLQIVLIFVVACIAGMGSILDEFQFHRPLVACTLIGLVLDDMKTGIIIGGTLEMIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHH
LGWMNIGAAVAPDAALASIISTILVIAGNQSVGAGIALAIPLAATGQVLTIIVRTITVAF
HHHHHCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHCCCHHHHHHHHHHHHHH
QHAADSAAERGNMTALSWIHVSALVLQAMRIAIPAVIVGVSVGTDAVHNMLSSIPEIVTN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GLNIAGGMIMVVGYAMVINMMRTGYLMPFFYLGFVTAAFTSFNLVALGVIGVVMAILYIQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSPRYNRAAGQAAPAQGANDLDNELD
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]