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The map label for this gene is pykA [H]

Identifier: 85059246

GI number: 85059246

Start: 2132552

End: 2133994

Strand: Reverse

Name: pykA [H]

Synonym: SG1268

Alternate gene names: 85059246

Gene position: 2133994-2132552 (Counterclockwise)

Preceding gene: 85059247

Following gene: 85059244

Centisome position: 51.16

GC content: 58.56

Gene sequence:

>1443_bases
ATGTCCAGACGGCTAAGAAGAACCAAAATTGTCACAACCCTCGGCCCCGCCACCGATCGCAATAATAATCTCGAAAAAGT
GATCGCCGCCGGCGCCAACGTCGTACGCCTGAACTTTTCACACGGCAGCGCAAAAGATCATCTTTTGCGAGCGCAGAAAG
TGCGCGAAATCTCGGCCCGACTCGGCCGGCACGTGGCGATACTCGGCGATTTGCAAGGGCCTAAAATCCGCGTTTCGACG
TTCCGTGAAGGGAAAATTTTTTTGAACATCGGCGACAAATTCCTGCTGGATGCCTCACTTGGCCGCGGCGAGGGCGACCG
CGAGCGGGTGGGCATTGATTATAAGGGCTTGCCCGACGATGTGGTGCCCGGCGATATTCTGCTGCTCGATGATGGCCGCG
TGCAGCTCAAAATACTCGAAGTCCAGGGTATTCGGGTTTACACCGAGGTGACGGTAGGCGGTCCGCTGTCCAATAACAAG
GGCATCAACAAGCTTGGCGGCGGTTTGTCCGCCGAAACGCTGACCGAAAAAGACAAAGCCGACATCATTACCGCCGCGCG
AATTGGCGTTGATTACCTGGCGGTGTCCTTTCCCCGCACCGGCGAGGATTTGAACTATGCCCGCCGTCTGGCTCGTGACG
CGGGCAGCTATGCCAAAATTGTCTCGAAGGTGGAGCGCGCCGAAGCCGTGGCGACCGAGGAAGCGATGGACGATATCATC
CTCGCCTCGGATGTGGTGATGGTCGCCCGCGGCGATTTAGGGGTTGAAATTGGCGATCCGGAACTGGTGGGTATTCAAAA
AAAATTGATCCGCCGCGCCCGTACGCTTAATCGCGCCGTCATCACCGCCACGCAGATGATGGAGTCGATGATAACCAATC
CGATGCCGACGCGCGCGGAAGTCATGGACGTCGCCAACGCGGTGCTCGACGGCACCGATGCGGTCATGCTCTCGGCCGAG
ACCGCCGCAGGGCAGTATCCCGCCGAAACGGTGGCCGCAATGGCCAGCGTCTGCCTCGGCGCGGAAAAGATCCCCAGCAT
CAATGTGTCCAAACATCGGCTGGATGTGCAATTCGATAACATCGAAGAGGCCATCGCCATGTCGGCGATGTACGCCGCCA
ACCACCTGAAAGGCATCACCGCCATTATCTCAATGACAGAATCGGGCCGCACCGCGCTTATGATGTCGCGTATCAGCTCG
GGTCTGCCGATTTTTGCCCTCTCGCGCCACGAACATACCCTGAATCTGACCGCGCTTTATCGCGGCGTGACGCCGGTCTA
CTTTGACGGCGACGGTGAAGGCGTGGCGGCGACGACCCACGCCACCAACTTGCTGCGCGATAAAGGCTTTCTGGTGTCGG
GAGACACGGTCATTATCACCCAGGGCGATGTCATGGACATGGTCGGGACCACCAACACCAGCCGCATCCTGCGCGTGGAA
TAA

Upstream 100 bases:

>100_bases
CAGGGATTACCCGGCGCGGGGACCGGTCAAACGTCTGGCACCCCTCCGGAGCAGTCTTTTTGTTGTAACAACACCATTGC
CCAAGTCAACGGAGTTATCC

Downstream 100 bases:

>100_bases
TCGCCCGACAAACGCATCACGGGCCGCATCGGGCGGCCGTGATGTCCATCGCGGTGGGTTGTACGCGCATCGCGCGCAGC
GCTGATAGACGCCGCAAATA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSRRLRRTKIVTTLGPATDRNNNLEKVIAAGANVVRLNFSHGSAKDHLLRAQKVREISARLGRHVAILGDLQGPKIRVST
FREGKIFLNIGDKFLLDASLGRGEGDRERVGIDYKGLPDDVVPGDILLLDDGRVQLKILEVQGIRVYTEVTVGGPLSNNK
GINKLGGGLSAETLTEKDKADIITAARIGVDYLAVSFPRTGEDLNYARRLARDAGSYAKIVSKVERAEAVATEEAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMASVCLGAEKIPSINVSKHRLDVQFDNIEEAIAMSAMYAANHLKGITAIISMTESGRTALMMSRISS
GLPIFALSRHEHTLNLTALYRGVTPVYFDGDGEGVAATTHATNLLRDKGFLVSGDTVIITQGDVMDMVGTTNTSRILRVE

Sequences:

>Translated_480_residues
MSRRLRRTKIVTTLGPATDRNNNLEKVIAAGANVVRLNFSHGSAKDHLLRAQKVREISARLGRHVAILGDLQGPKIRVST
FREGKIFLNIGDKFLLDASLGRGEGDRERVGIDYKGLPDDVVPGDILLLDDGRVQLKILEVQGIRVYTEVTVGGPLSNNK
GINKLGGGLSAETLTEKDKADIITAARIGVDYLAVSFPRTGEDLNYARRLARDAGSYAKIVSKVERAEAVATEEAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMASVCLGAEKIPSINVSKHRLDVQFDNIEEAIAMSAMYAANHLKGITAIISMTESGRTALMMSRISS
GLPIFALSRHEHTLNLTALYRGVTPVYFDGDGEGVAATTHATNLLRDKGFLVSGDTVIITQGDVMDMVGTTNTSRILRVE
>Mature_479_residues
SRRLRRTKIVTTLGPATDRNNNLEKVIAAGANVVRLNFSHGSAKDHLLRAQKVREISARLGRHVAILGDLQGPKIRVSTF
REGKIFLNIGDKFLLDASLGRGEGDRERVGIDYKGLPDDVVPGDILLLDDGRVQLKILEVQGIRVYTEVTVGGPLSNNKG
INKLGGGLSAETLTEKDKADIITAARIGVDYLAVSFPRTGEDLNYARRLARDAGSYAKIVSKVERAEAVATEEAMDDIIL
ASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPAETVAAMASVCLGAEKIPSINVSKHRLDVQFDNIEEAIAMSAMYAANHLKGITAIISMTESGRTALMMSRISSG
LPIFALSRHEHTLNLTALYRGVTPVYFDGDGEGVAATTHATNLLRDKGFLVSGDTVIITQGDVMDMVGTTNTSRILRVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=499, Percent_Identity=35.6713426853707, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI33286420, Length=499, Percent_Identity=35.6713426853707, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=35.959595959596, Blast_Score=242, Evalue=7e-64,
Organism=Homo sapiens, GI10835121, Length=501, Percent_Identity=34.5309381237525, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI32967597, Length=501, Percent_Identity=34.5309381237525, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=33.7931034482759, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=33.7931034482759, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=31.7796610169492, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=31.7796610169492, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=31.1627906976744, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=87.7083333333333, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=487, Percent_Identity=37.5770020533881, Blast_Score=280, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=490, Percent_Identity=35.1020408163265, Blast_Score=261, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=36.2348178137652, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=36.2348178137652, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=36.2348178137652, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=36.2348178137652, Blast_Score=232, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6324923, Length=496, Percent_Identity=35.2822580645161, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=475, Percent_Identity=34.9473684210526, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI28571814, Length=501, Percent_Identity=36.127744510978, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24648964, Length=501, Percent_Identity=36.127744510978, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24648966, Length=421, Percent_Identity=35.1543942992874, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24581235, Length=497, Percent_Identity=31.7907444668008, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24646914, Length=289, Percent_Identity=33.5640138408304, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51690; Mature: 51559

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVTTLGPATDRNNNLEKVIAAGANVVRLNFSHGSAKDHLLRAQKVREISAR
CCCCCCHHEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFREGKIFLNIGDKFLLDASLGRGEGDRERVGIDYKGLPDD
HCCEEEEEECCCCCEEEEEEEECCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCC
VVPGDILLLDDGRVQLKILEVQGIRVYTEVTVGGPLSNNKGINKLGGGLSAETLTEKDKA
CCCCCEEEEECCCEEEEEEEEECEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCCHH
DIITAARIGVDYLAVSFPRTGEDLNYARRLARDAGSYAKIVSKVERAEAVATEEAMDDII
CEEEHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMASVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
IEEAIAMSAMYAANHLKGITAIISMTESGRTALMMSRISSGLPIFALSRHEHTLNLTALY
HHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEEE
RGVTPVYFDGDGEGVAATTHATNLLRDKGFLVSGDTVIITQGDVMDMVGTTNTSRILRVE
CCCCEEEECCCCCCEEEEHHHHHHHHCCCEEEECCEEEEECCCEEEHHCCCCCCEEEEEC
>Mature Secondary Structure 
SRRLRRTKIVTTLGPATDRNNNLEKVIAAGANVVRLNFSHGSAKDHLLRAQKVREISAR
CCCCCHHEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFREGKIFLNIGDKFLLDASLGRGEGDRERVGIDYKGLPDD
HCCEEEEEECCCCCEEEEEEEECCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCC
VVPGDILLLDDGRVQLKILEVQGIRVYTEVTVGGPLSNNKGINKLGGGLSAETLTEKDKA
CCCCCEEEEECCCEEEEEEEEECEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCCHH
DIITAARIGVDYLAVSFPRTGEDLNYARRLARDAGSYAKIVSKVERAEAVATEEAMDDII
CEEEHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMASVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
IEEAIAMSAMYAANHLKGITAIISMTESGRTALMMSRISSGLPIFALSRHEHTLNLTALY
HHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEEE
RGVTPVYFDGDGEGVAATTHATNLLRDKGFLVSGDTVIITQGDVMDMVGTTNTSRILRVE
CCCCEEEECCCCCCEEEEHHHHHHHHCCCEEEECCEEEEECCCEEEHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]