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The map label for this gene is hisH

Identifier: 85059104

GI number: 85059104

Start: 1868623

End: 1869213

Strand: Reverse

Name: hisH

Synonym: SG1126

Alternate gene names: 85059104

Gene position: 1869213-1868623 (Counterclockwise)

Preceding gene: 85059105

Following gene: 85059103

Centisome position: 44.81

GC content: 61.59

Gene sequence:

>591_bases
ATGAACGTGGTTGTGCTGGATACCGGCTGCGCCAATCTGTTCTCGGTCACCGCTGCGATACGTCGCCTGGGCTATGAACC
GGTCGTCAGCAGCAACGCCGACATCGTGTTGCAGGCGGACAAATTATTTCTGCCCGGCGTCGGCACCGCCCAGGCGGCCA
TGGATCAGTTGCGCGAGCGGGAACTCATTGCGCTGATTAAAACCTGCGCCCAGCAGGTGTTGGGCATTTGCCTCGGCATG
CAGTTGCTGGGCATCGCCAGCGATGAAAATGGCGGCGTCAGCACCCTCGGCATTATCGACGCGCCGGTCACGCGCATGGC
GGACGCCGGTCTGCCGCTGCCCCATATGGGCTGGAACCAAGTGACCGCCCAGGCCAGCAATCGCCTGTTCCGCGGCATTG
ACGACGGCAGCTATTTTTATTTCGTCCACGGTTATGCAATGCCGCTGTGCGCCGCCACCATCGCCCATGCCAGCTATGGC
GCGCCGTTTACCGCCGCGGTGGAGCAGGATAATTTCTTCGGCGTGCAGTTTCACCCGGAGCGCTCCGGCGCCGCCGGCCG
CCAGCTGCTGAAAAATTTTTTGGAGATGTAA

Upstream 100 bases:

>100_bases
ATCATCGGGTGGAGAGCCTGTTTAAAGCCTTCGGCCGCACGCTGCGCCAGGCCATCCGCGTGGAGGGCGACACGCTGCCC
AGCTCAAAAGGAGTGCTGTG

Downstream 100 bases:

>100_bases
CCGGTGATTATACCCGCTTTGGATTTGATTGATGGAGCCGTCGTGCGGCTCCATCAGGGCGATTATCAGACACAATGCCA
CTACGGCGCCGATCCGCTGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNVVVLDTGCANLFSVTAAIRRLGYEPVVSSNADIVLQADKLFLPGVGTAQAAMDQLRERELIALIKTCAQQVLGICLGM
QLLGIASDENGGVSTLGIIDAPVTRMADAGLPLPHMGWNQVTAQASNRLFRGIDDGSYFYFVHGYAMPLCAATIAHASYG
APFTAAVEQDNFFGVQFHPERSGAAGRQLLKNFLEM

Sequences:

>Translated_196_residues
MNVVVLDTGCANLFSVTAAIRRLGYEPVVSSNADIVLQADKLFLPGVGTAQAAMDQLRERELIALIKTCAQQVLGICLGM
QLLGIASDENGGVSTLGIIDAPVTRMADAGLPLPHMGWNQVTAQASNRLFRGIDDGSYFYFVHGYAMPLCAATIAHASYG
APFTAAVEQDNFFGVQFHPERSGAAGRQLLKNFLEM
>Mature_196_residues
MNVVVLDTGCANLFSVTAAIRRLGYEPVVSSNADIVLQADKLFLPGVGTAQAAMDQLRERELIALIKTCAQQVLGICLGM
QLLGIASDENGGVSTLGIIDAPVTRMADAGLPLPHMGWNQVTAQASNRLFRGIDDGSYFYFVHGYAMPLCAATIAHASYG
APFTAAVEQDNFFGVQFHPERSGAAGRQLLKNFLEM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=73.469387755102, Blast_Score=300, Evalue=5e-83,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=37.3205741626794, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 20936; Mature: 20936

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVVLDTGCANLFSVTAAIRRLGYEPVVSSNADIVLQADKLFLPGVGTAQAAMDQLRER
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHH
ELIALIKTCAQQVLGICLGMQLLGIASDENGGVSTLGIIDAPVTRMADAGLPLPHMGWNQ
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHH
VTAQASNRLFRGIDDGSYFYFVHGYAMPLCAATIAHASYGAPFTAAVEQDNFFGVQFHPE
HHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCC
RSGAAGRQLLKNFLEM
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNVVVLDTGCANLFSVTAAIRRLGYEPVVSSNADIVLQADKLFLPGVGTAQAAMDQLRER
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHH
ELIALIKTCAQQVLGICLGMQLLGIASDENGGVSTLGIIDAPVTRMADAGLPLPHMGWNQ
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHH
VTAQASNRLFRGIDDGSYFYFVHGYAMPLCAATIAHASYGAPFTAAVEQDNFFGVQFHPE
HHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCC
RSGAAGRQLLKNFLEM
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]