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The map label for this gene is ompF [H]

Identifier: 85058985

GI number: 85058985

Start: 1659845

End: 1660951

Strand: Reverse

Name: ompF [H]

Synonym: SG1007

Alternate gene names: 85058985

Gene position: 1660951-1659845 (Counterclockwise)

Preceding gene: 85058987

Following gene: 85058984

Centisome position: 39.82

GC content: 53.48

Gene sequence:

>1107_bases
ATGATAAAGCGCAATCTTTTTGCAGTGGTAATTCCGGCCTTATTGGCTGCCGGTGCCGCTAACGCGGCCGAAGTGTATAA
CAAAGATGGCAATAAATTAGACATCTACGGTAAGGCTGTCGGCCTGCACTACTTCTCCCACAGCGATAACAGCTACGGCG
GTGACGGCGACCAGACTTATGCCCGTCTCGGCTTCAAAGGTGAAACTCAGATTACCGACCAGCTGACCGGTTTCGGCCAG
TGGGAATACAACTTCCAGGGCAACAACTCCGAAAGCAACACCGACGCTCAGGACGGCAACAAAACCCGTCTGGCGTTCGC
CGGTCTGAAATTCGGCGATGCCGGTTCATTCGACTACGGCCGTAACTACGGCGTAGTGTATGACGCTATCGGCTGGACCG
ACATGCTGCCGGAATTCGGCGGTGACTCTGGCTACAGCGACAACTTCATGATGGGTCGTACCACCGGCGTTGCTACCTAC
CGTAACACCAACTTCTTCGGCTTGGTAGACGGCTGGGATTTCGCAGTACAGTACCAGGGCAAGAACGATCGTGACGAAAT
CCGTCGTGCCAACGGCGACGGCTGGGGCGCGTCTACCAGCTACACCTCCCCCATCGGGGTTGGCATCGTTGGTGCTTATG
CTTCATCCGACCGTACCGATGCACAGCAGAGCGCTTACTACGCTAACGGCAGCGAAAAAGCTCAGCAGTGGGCTGCCGGT
CTGAAATATGACGCCAACAACGTTTACCTGGCTGCCATGTACGGTGAAACCCGTAACGCAACCTATATCACCAACCCCAA
CCTGCAGATCAATGGCGCTGATGCCACAGGTTTTGCTAACAAAACCCAGCTGATTGAATTGGTTGCTCAGTACCAATTCG
ACTTCGGTCTGCGTCCGTCTGTTGCCTACGTTCAGTCGAAAGGTAAAGACATAGAAGGCGTGGGTGATGTTAACCTGATC
AAATACGTTGAAGTGGGCGCTACCTACTACTTCAACAAAAACATGTCCACCTATGTTGACTATATCATCAACCAGATTGA
TAGCGACAACGAGCTGGGCATCGGCAGCGGCGACACCGTTGCTCTGGGTCTGGTCTACCAGTTCTAA

Upstream 100 bases:

>100_bases
CGTGCAGACACAGGAAGACACCAAACTCTCAATAATGTTTCGTAAGAAATTATTAGCGGCAGTAGTAGGTGTCTGAATAA
CACTAATGAGGGTAGTAATA

Downstream 100 bases:

>100_bases
TCCTTTTCCAGCCGCTTACAGCTGAAAGTCTGGATACTGAAGGCAGGGTTTATACCCTGCCTTCTTTTTTTATGGCCGCC
GAAAAATGTGCGGCAAACGG

Product: outer membrane protein F

Products: NA

Alternate protein names: Outer membrane protein 1A; Outer membrane protein B; Outer membrane protein IA; Porin OmpF [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MIKRNLFAVVIPALLAAGAANAAEVYNKDGNKLDIYGKAVGLHYFSHSDNSYGGDGDQTYARLGFKGETQITDQLTGFGQ
WEYNFQGNNSESNTDAQDGNKTRLAFAGLKFGDAGSFDYGRNYGVVYDAIGWTDMLPEFGGDSGYSDNFMMGRTTGVATY
RNTNFFGLVDGWDFAVQYQGKNDRDEIRRANGDGWGASTSYTSPIGVGIVGAYASSDRTDAQQSAYYANGSEKAQQWAAG
LKYDANNVYLAAMYGETRNATYITNPNLQINGADATGFANKTQLIELVAQYQFDFGLRPSVAYVQSKGKDIEGVGDVNLI
KYVEVGATYYFNKNMSTYVDYIINQIDSDNELGIGSGDTVALGLVYQF

Sequences:

>Translated_368_residues
MIKRNLFAVVIPALLAAGAANAAEVYNKDGNKLDIYGKAVGLHYFSHSDNSYGGDGDQTYARLGFKGETQITDQLTGFGQ
WEYNFQGNNSESNTDAQDGNKTRLAFAGLKFGDAGSFDYGRNYGVVYDAIGWTDMLPEFGGDSGYSDNFMMGRTTGVATY
RNTNFFGLVDGWDFAVQYQGKNDRDEIRRANGDGWGASTSYTSPIGVGIVGAYASSDRTDAQQSAYYANGSEKAQQWAAG
LKYDANNVYLAAMYGETRNATYITNPNLQINGADATGFANKTQLIELVAQYQFDFGLRPSVAYVQSKGKDIEGVGDVNLI
KYVEVGATYYFNKNMSTYVDYIINQIDSDNELGIGSGDTVALGLVYQF
>Mature_368_residues
MIKRNLFAVVIPALLAAGAANAAEVYNKDGNKLDIYGKAVGLHYFSHSDNSYGGDGDQTYARLGFKGETQITDQLTGFGQ
WEYNFQGNNSESNTDAQDGNKTRLAFAGLKFGDAGSFDYGRNYGVVYDAIGWTDMLPEFGGDSGYSDNFMMGRTTGVATY
RNTNFFGLVDGWDFAVQYQGKNDRDEIRRANGDGWGASTSYTSPIGVGIVGAYASSDRTDAQQSAYYANGSEKAQQWAAG
LKYDANNVYLAAMYGETRNATYITNPNLQINGADATGFANKTQLIELVAQYQFDFGLRPSVAYVQSKGKDIEGVGDVNLI
KYVEVGATYYFNKNMSTYVDYIINQIDSDNELGIGSGDTVALGLVYQF

Specific function: OmpF is a porin that forms passive diffusion pores which allow small molecular weight hydrophilic materials across the outer membrane. It is also a receptor for the bacteriophage T2 [H]

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1787160, Length=370, Percent_Identity=72.972972972973, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1786436, Length=369, Percent_Identity=57.7235772357724, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1788544, Length=387, Percent_Identity=59.6899224806201, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1787641, Length=371, Percent_Identity=60.6469002695418, Blast_Score=403, Evalue=1e-114,

Paralogues:

None

Copy number: 1980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 17600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12744 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 40086; Mature: 40086

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00576 GRAM_NEG_PORIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRNLFAVVIPALLAAGAANAAEVYNKDGNKLDIYGKAVGLHYFSHSDNSYGGDGDQTY
CCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCEE
ARLGFKGETQITDQLTGFGQWEYNFQGNNSESNTDAQDGNKTRLAFAGLKFGDAGSFDYG
EEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECEECCCCCCCCC
RNYGVVYDAIGWTDMLPEFGGDSGYSDNFMMGRTTGVATYRNTNFFGLVDGWDFAVQYQG
CCCCEEEECCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCEEEEECCCEEEEEECC
KNDRDEIRRANGDGWGASTSYTSPIGVGIVGAYASSDRTDAQQSAYYANGSEKAQQWAAG
CCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHEEECCCHHHHHHHHCC
LKYDANNVYLAAMYGETRNATYITNPNLQINGADATGFANKTQLIELVAQYQFDFGLRPS
CEECCCCEEEEEEECCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
VAYVQSKGKDIEGVGDVNLIKYVEVGATYYFNKNMSTYVDYIINQIDSDNELGIGSGDTV
CHHHHCCCCCCCCCCCCEEEEEEEECEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEE
ALGLVYQF
EEEEEEEC
>Mature Secondary Structure
MIKRNLFAVVIPALLAAGAANAAEVYNKDGNKLDIYGKAVGLHYFSHSDNSYGGDGDQTY
CCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCEE
ARLGFKGETQITDQLTGFGQWEYNFQGNNSESNTDAQDGNKTRLAFAGLKFGDAGSFDYG
EEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECEECCCCCCCCC
RNYGVVYDAIGWTDMLPEFGGDSGYSDNFMMGRTTGVATYRNTNFFGLVDGWDFAVQYQG
CCCCEEEECCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCEEEEECCCEEEEEECC
KNDRDEIRRANGDGWGASTSYTSPIGVGIVGAYASSDRTDAQQSAYYANGSEKAQQWAAG
CCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHEEECCCHHHHHHHHCC
LKYDANNVYLAAMYGETRNATYITNPNLQINGADATGFANKTQLIELVAQYQFDFGLRPS
CEECCCCEEEEEEECCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
VAYVQSKGKDIEGVGDVNLIKYVEVGATYYFNKNMSTYVDYIINQIDSDNELGIGSGDTV
CHHHHCCCCCCCCCCCCEEEEEEEECEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEE
ALGLVYQF
EEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6294623; 8905232; 9278503; 7037455; 7049161; 2997131; 9298646; 9629924; 1380671; 7524100; 9843370 [H]