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The map label for this gene is gltA

Identifier: 85058849

GI number: 85058849

Start: 1426648

End: 1427928

Strand: Reverse

Name: gltA

Synonym: SG0871

Alternate gene names: 85058849

Gene position: 1427928-1426648 (Counterclockwise)

Preceding gene: 85058869

Following gene: 85058842

Centisome position: 34.23

GC content: 56.6

Gene sequence:

>1281_bases
ATGGCTAAAAGTAAAGCGACTTTTACCCCTGAGGGGCAGGACCCTATCGCCATGGATATGCTAACGGGCACCATGGGCGA
AAAAGAAATCGATATCCGCAGCCTGGGCAGCCAGGGGTATTTCACCTTCGATCCCGGCTTCACCTCCACCGCCTCCTGTG
AATCTAAGATCACCTATATCGATGGCGACAAAGGCGTCTTGCTGCATCGCGGCTTTCCCATCGACAAGCTGGCGCTGGAG
TCGAACTATCTGGAGGTCTGCTATATCCTCCTGAACGGCGAAGCGCCGACCCGCGAGCAATATGATGAGTTTCGCACTAT
CGTCACGCGCCATACCATGATTCATGAACAGATCACCCGGCTATTCCACGGTTTTCGGCGCGACTCCCATCCGATGGCGG
TCATGTGCGGAGTGACCGGCGCACTGGCGGCGTTCTATCATGACTCGCTGGATGTCAACGTTGAACGCCATCGCGAAATC
GCCGCGTTTCGCCTGCTGTCGAAAATGCCCACCGTAGCCGCCATGTGCTACAAGTATTCGCTCGGCCAGCCATTTATCTA
CCCGCGCAACGATCTGTCCTATGCCGGCAACTTCCTGCATATGATGTTCTCCACGCCGTGCGAAACCTATGAGGTTAATC
CGGTGCTGGAACGCGCCATGGATCGTATCCTCATTCTGCACGCCGACCACGAACAGAACGCGTCTACCTCAACGGTGCGC
ACCGCCGGCTCTTCGGGCGCCAATCCGTTCGCCTGTATCGCGGCAGGCATTGCCTCGTTATGGGGACCTGCCCACGGCGG
CGCCAACGAAGCCTGCCTACGGATGCTTGAGGAGATCAACAAGGTGGAGCATATCCCCGAGTTCATCAAGCGCGCCAAGG
ATAAAAACGATTCGTTCCGCCTGATGGGCTTTGGCCACCGCGTGTACAAAAATTACGATCCGCGCGCGACCGTGATGCGC
GAAACCTGCCATGAAGTATTGAAAGAGCTTAAGCTTTCCGATGACCTGCTGGCGGTGGCCATGGAGCTCGAACACATCGC
GCTGCACGACCCCTACTTCAAAGAGCGCAAACTCTATCCCAACGTTGACTTCTACTCCGGCATCATTCTGAAAGCCATGG
GGATTCCTTCAACCATGTTCACGGTCATTTTCGCCATGGCGCGCACTGTCGGCTGGATAGCCCACTGGAAAGAAATGCAC
GACGACGGGCTGAAAATCGCCCGTCCGCGCCAGCTCTACACCGGCTATGCCCAGCGGGATTTCCACAGCCAGCGCAAATA
A

Upstream 100 bases:

>100_bases
AGGCTTATCGGCATGTTCGGCACGGACGGTAGAAAAAACGCCGGGGTCAGTAAGCTGCCCGTGGTGAAAGATTGAGTTTA
TACGCGCTAAGGAGAATATA

Downstream 100 bases:

>100_bases
ACCTGTCGTGCCGGGGGGGGGGACCTGCACCACACCGGGCAACACGCCGTGGGACAGAAGCTCCCGGTCGGCGGTGCGCT
GTAGGACGCTGCCCAGCAGA

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MAKSKATFTPEGQDPIAMDMLTGTMGEKEIDIRSLGSQGYFTFDPGFTSTASCESKITYIDGDKGVLLHRGFPIDKLALE
SNYLEVCYILLNGEAPTREQYDEFRTIVTRHTMIHEQITRLFHGFRRDSHPMAVMCGVTGALAAFYHDSLDVNVERHREI
AAFRLLSKMPTVAAMCYKYSLGQPFIYPRNDLSYAGNFLHMMFSTPCETYEVNPVLERAMDRILILHADHEQNASTSTVR
TAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEINKVEHIPEFIKRAKDKNDSFRLMGFGHRVYKNYDPRATVMR
ETCHEVLKELKLSDDLLAVAMELEHIALHDPYFKERKLYPNVDFYSGIILKAMGIPSTMFTVIFAMARTVGWIAHWKEMH
DDGLKIARPRQLYTGYAQRDFHSQRK

Sequences:

>Translated_426_residues
MAKSKATFTPEGQDPIAMDMLTGTMGEKEIDIRSLGSQGYFTFDPGFTSTASCESKITYIDGDKGVLLHRGFPIDKLALE
SNYLEVCYILLNGEAPTREQYDEFRTIVTRHTMIHEQITRLFHGFRRDSHPMAVMCGVTGALAAFYHDSLDVNVERHREI
AAFRLLSKMPTVAAMCYKYSLGQPFIYPRNDLSYAGNFLHMMFSTPCETYEVNPVLERAMDRILILHADHEQNASTSTVR
TAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEINKVEHIPEFIKRAKDKNDSFRLMGFGHRVYKNYDPRATVMR
ETCHEVLKELKLSDDLLAVAMELEHIALHDPYFKERKLYPNVDFYSGIILKAMGIPSTMFTVIFAMARTVGWIAHWKEMH
DDGLKIARPRQLYTGYAQRDFHSQRK
>Mature_425_residues
AKSKATFTPEGQDPIAMDMLTGTMGEKEIDIRSLGSQGYFTFDPGFTSTASCESKITYIDGDKGVLLHRGFPIDKLALES
NYLEVCYILLNGEAPTREQYDEFRTIVTRHTMIHEQITRLFHGFRRDSHPMAVMCGVTGALAAFYHDSLDVNVERHREIA
AFRLLSKMPTVAAMCYKYSLGQPFIYPRNDLSYAGNFLHMMFSTPCETYEVNPVLERAMDRILILHADHEQNASTSTVRT
AGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEINKVEHIPEFIKRAKDKNDSFRLMGFGHRVYKNYDPRATVMRE
TCHEVLKELKLSDDLLAVAMELEHIALHDPYFKERKLYPNVDFYSGIILKAMGIPSTMFTVIFAMARTVGWIAHWKEMHD
DGLKIARPRQLYTGYAQRDFHSQRK

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=404, Percent_Identity=25.7425742574257, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786939, Length=426, Percent_Identity=84.7417840375587, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1786527, Length=364, Percent_Identity=32.1428571428571, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17555174, Length=348, Percent_Identity=27.0114942528736, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319850, Length=348, Percent_Identity=26.7241379310345, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324328, Length=343, Percent_Identity=27.4052478134111, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325257, Length=453, Percent_Identity=24.9448123620309, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24640124, Length=424, Percent_Identity=26.1792452830189, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24640126, Length=424, Percent_Identity=26.1792452830189, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356863, Length=395, Percent_Identity=24.3037974683544, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48291; Mature: 48160

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKATFTPEGQDPIAMDMLTGTMGEKEIDIRSLGSQGYFTFDPGFTSTASCESKITYI
CCCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCEEEEE
DGDKGVLLHRGFPIDKLALESNYLEVCYILLNGEAPTREQYDEFRTIVTRHTMIHEQITR
ECCCCEEEECCCCCHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFHGFRRDSHPMAVMCGVTGALAAFYHDSLDVNVERHREIAAFRLLSKMPTVAAMCYKYS
HHHHHCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
LGQPFIYPRNDLSYAGNFLHMMFSTPCETYEVNPVLERAMDRILILHADHEQNASTSTVR
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHHHCCEEEEECCCCCCCCCHHHE
TAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEINKVEHIPEFIKRAKDKNDSFR
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LMGFGHRVYKNYDPRATVMRETCHEVLKELKLSDDLLAVAMELEHIALHDPYFKERKLYP
EEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHCCCCCC
NVDFYSGIILKAMGIPSTMFTVIFAMARTVGWIAHWKEMHDDGLKIARPRQLYTGYAQRD
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHH
FHSQRK
HHHCCC
>Mature Secondary Structure 
AKSKATFTPEGQDPIAMDMLTGTMGEKEIDIRSLGSQGYFTFDPGFTSTASCESKITYI
CCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCEEEEE
DGDKGVLLHRGFPIDKLALESNYLEVCYILLNGEAPTREQYDEFRTIVTRHTMIHEQITR
ECCCCEEEECCCCCHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFHGFRRDSHPMAVMCGVTGALAAFYHDSLDVNVERHREIAAFRLLSKMPTVAAMCYKYS
HHHHHCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
LGQPFIYPRNDLSYAGNFLHMMFSTPCETYEVNPVLERAMDRILILHADHEQNASTSTVR
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHHHCCEEEEECCCCCCCCCHHHE
TAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEINKVEHIPEFIKRAKDKNDSFR
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LMGFGHRVYKNYDPRATVMRETCHEVLKELKLSDDLLAVAMELEHIALHDPYFKERKLYP
EEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHCCCCCC
NVDFYSGIILKAMGIPSTMFTVIFAMARTVGWIAHWKEMHDDGLKIARPRQLYTGYAQRD
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHH
FHSQRK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]