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The map label for this gene is leuC

Identifier: 85058413

GI number: 85058413

Start: 764535

End: 765935

Strand: Reverse

Name: leuC

Synonym: SG0435

Alternate gene names: 85058413

Gene position: 765935-764535 (Counterclockwise)

Preceding gene: 162382797

Following gene: 85058412

Centisome position: 18.36

GC content: 59.6

Gene sequence:

>1401_bases
ATGGGCAAGTCGTTATACCAAAAATTATATGATGCACACGTGGTTTATGAAGCGGAGAACGAAACGCCGCTTTTGTATAT
CGACCGCCATTTGGTGCATGAAGTCACCTCGCCGCAAGCGTTTGACGGCTTGCGCGCCCATGGCCGCACCGTACGTCAAC
CGGGCAAAACCTTCGCCACCATGGACCACAACGTTTCCACGCAAACCAAAGACATCAACGCCTGTGGTGATATGGCCCGC
ATCCAGATGCAGGAGCTGATGAAAAACTGCGCGGAATTCGGCGTGCAGCTGTATGACCTGAACCACCCTTATCAAGGGAT
CGTGCACGTCATCGGTCCGGAACAAGGGATGATCTTGCCGGGCATGACTATCGTCTGCGGCGATTCCCATACTGCCACCC
ACGGCGCCTTTGGCGCGCTGGCGTTCGGTATCGGCACCTCAGAAATAGAACACGTACTGGCGACCCAGACCCTGAAACAG
GGCCGTGCCAAAACCATGAAAGTGGACATGACCGGCCACGCCTCCCCCGGTATTACCGCCAAGGATATTGTCCTGGCGAT
CATCAGTAAAATCGGTTCAGCTGGCGGTACCGGCCACGTGGTGGAATTCAGCGGCTCCGCCATTCGCGCGCTTAGCATGG
AAGGCCGCATGACGCTTTGCAATATGGCGATCGAAATGGGCGCCAAGGCGGGCCTGGTCGCCCCGGACGACACCACCTAC
GCCTACCTGCAAAACCGGCAGTTTGCGCCCAAGGGCGATGACTGGCATCAGGCCGTGATGTATTGGGATACGCTGAAATC
CGACGATGATGCCCGCTTCGACAAAGTCGTGACGCTGGATGCGCAGGACATCGCGCCGCAGGTGACCTGGGGCACCAACC
CCGGCCAGGTCATTGCCGTCAATCAGGTCATCCCGTCGCCGGAGTCATTCAGCGATCCGGTAGAGCGTAATTCTGCCGCC
AAGGCACTGGCCTATATGGATTTGCAGCCCGGCATTCGTCTGACCGACGTGACGATTGACAAGGTCTTCATTGGTTCTTG
CACCAACTCACGCATCGAAGATCTGCGCGCCGCCGCCGCTGTTGCCCGCGGTCGTCGCGTTGCCGCCGGCGTACAGGCAA
TCGTGGTCCCCGGCTCCGGTCCGGTTAAGGCGCAGGCCGAAGCGGAAGGGCTGGATAGAATTTTCCTCGATGCCGGGTTT
GAATGGCGCCTGCCGGGCTGCTCCATGTGCCTGGCGATGAACAACGACAGCCTGAACCCCGGTGAGCGCTGCGCGTCAAC
CAGCAATCGTAACTTTGAAGGCCGTCAGGGCCGCGGCGGACGCACCCATCTGGTCAGCCCCGCGATGGCGGCGGCGGCGG
CGGTAGCCGGCCGTTTCGCCGATATCCGCGAATTAAATTAA

Upstream 100 bases:

>100_bases
GACACCGCACCGCCGACCTGGCCGGCGGCGGCAGCGCGGTAGGTACCGGTGAAATGGGCGATATTATCGCCGCGTTAATC
AGTCAAGGGGCATAATCAAC

Downstream 100 bases:

>100_bases
GGAAAAAGAGATGGCTAAATTTACCCAACATACCGGCATCGTGGTGCCCCTTGACGCCGCCAACGTCGACACCGATGCCA
TCATCCCCAAGCAGTTTCTG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGKSLYQKLYDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRTVRQPGKTFATMDHNVSTQTKDINACGDMAR
IQMQELMKNCAEFGVQLYDLNHPYQGIVHVIGPEQGMILPGMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLKQ
GRAKTMKVDMTGHASPGITAKDIVLAIISKIGSAGGTGHVVEFSGSAIRALSMEGRMTLCNMAIEMGAKAGLVAPDDTTY
AYLQNRQFAPKGDDWHQAVMYWDTLKSDDDARFDKVVTLDAQDIAPQVTWGTNPGQVIAVNQVIPSPESFSDPVERNSAA
KALAYMDLQPGIRLTDVTIDKVFIGSCTNSRIEDLRAAAAVARGRRVAAGVQAIVVPGSGPVKAQAEAEGLDRIFLDAGF
EWRLPGCSMCLAMNNDSLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVAGRFADIRELN

Sequences:

>Translated_466_residues
MGKSLYQKLYDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRTVRQPGKTFATMDHNVSTQTKDINACGDMAR
IQMQELMKNCAEFGVQLYDLNHPYQGIVHVIGPEQGMILPGMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLKQ
GRAKTMKVDMTGHASPGITAKDIVLAIISKIGSAGGTGHVVEFSGSAIRALSMEGRMTLCNMAIEMGAKAGLVAPDDTTY
AYLQNRQFAPKGDDWHQAVMYWDTLKSDDDARFDKVVTLDAQDIAPQVTWGTNPGQVIAVNQVIPSPESFSDPVERNSAA
KALAYMDLQPGIRLTDVTIDKVFIGSCTNSRIEDLRAAAAVARGRRVAAGVQAIVVPGSGPVKAQAEAEGLDRIFLDAGF
EWRLPGCSMCLAMNNDSLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVAGRFADIRELN
>Mature_465_residues
GKSLYQKLYDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRTVRQPGKTFATMDHNVSTQTKDINACGDMARI
QMQELMKNCAEFGVQLYDLNHPYQGIVHVIGPEQGMILPGMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLKQG
RAKTMKVDMTGHASPGITAKDIVLAIISKIGSAGGTGHVVEFSGSAIRALSMEGRMTLCNMAIEMGAKAGLVAPDDTTYA
YLQNRQFAPKGDDWHQAVMYWDTLKSDDDARFDKVVTLDAQDIAPQVTWGTNPGQVIAVNQVIPSPESFSDPVERNSAAK
ALAYMDLQPGIRLTDVTIDKVFIGSCTNSRIEDLRAAAAVARGRRVAAGVQAIVVPGSGPVKAQAEAEGLDRIFLDAGFE
WRLPGCSMCLAMNNDSLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVAGRFADIRELN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=408, Percent_Identity=27.2058823529412, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI8659555, Length=396, Percent_Identity=27.5252525252525, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=26.3157894736842, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=83.6909871244635, Blast_Score=813, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=27.5204359673025, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87081781, Length=378, Percent_Identity=24.6031746031746, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=413, Percent_Identity=27.1186440677966, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=413, Percent_Identity=27.1186440677966, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=308, Percent_Identity=27.5974025974026, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=452, Percent_Identity=25.8849557522124, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=57.6595744680851, Blast_Score=565, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6320440, Length=424, Percent_Identity=26.1792452830189, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=412, Percent_Identity=27.9126213592233, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=387, Percent_Identity=26.8733850129199, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281365315, Length=413, Percent_Identity=25.90799031477, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17864292, Length=413, Percent_Identity=25.90799031477, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076999, Length=414, Percent_Identity=25.3623188405797, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28571643, Length=409, Percent_Identity=26.4058679706601, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24645686, Length=454, Percent_Identity=27.5330396475771, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17137564, Length=452, Percent_Identity=25.4424778761062, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SODGM (Q2NVW5)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454115.1
- ProteinModelPortal:   Q2NVW5
- SMR:   Q2NVW5
- STRING:   Q2NVW5
- GeneID:   3867410
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0435
- NMPDR:   fig|343509.6.peg.1005
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q2NVW5
- ProtClustDB:   PRK05478
- BioCyc:   SGLO343509:SG0435-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50071; Mature: 49940

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLYQKLYDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRTVRQPGKTFAT
CCHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEE
MDHNVSTQTKDINACGDMARIQMQELMKNCAEFGVQLYDLNHPYQGIVHVIGPEQGMILP
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCCCEEEC
GMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLKQGRAKTMKVDMTGHASPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCH
KDIVLAIISKIGSAGGTGHVVEFSGSAIRALSMEGRMTLCNMAIEMGAKAGLVAPDDTTY
HHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEE
AYLQNRQFAPKGDDWHQAVMYWDTLKSDDDARFDKVVTLDAQDIAPQVTWGTNPGQVIAV
EEEECCCCCCCCCCHHHHEEEEHHCCCCCCCCCCEEEEECHHHCCCEEEECCCCCCEEEE
NQVIPSPESFSDPVERNSAAKALAYMDLQPGIRLTDVTIDKVFIGSCTNSRIEDLRAAAA
EECCCCCCCCCCCHHHCCHHHEEEEEECCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHH
VARGRRVAAGVQAIVVPGSGPVKAQAEAEGLDRIFLDAGFEWRLPGCSMCLAMNNDSLNP
HHCCCEEECCEEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVAGRFADIRELN
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKSLYQKLYDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRTVRQPGKTFAT
CHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEE
MDHNVSTQTKDINACGDMARIQMQELMKNCAEFGVQLYDLNHPYQGIVHVIGPEQGMILP
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCCCEEEC
GMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLKQGRAKTMKVDMTGHASPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCH
KDIVLAIISKIGSAGGTGHVVEFSGSAIRALSMEGRMTLCNMAIEMGAKAGLVAPDDTTY
HHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEE
AYLQNRQFAPKGDDWHQAVMYWDTLKSDDDARFDKVVTLDAQDIAPQVTWGTNPGQVIAV
EEEECCCCCCCCCCHHHHEEEEHHCCCCCCCCCCEEEEECHHHCCCEEEECCCCCCEEEE
NQVIPSPESFSDPVERNSAAKALAYMDLQPGIRLTDVTIDKVFIGSCTNSRIEDLRAAAA
EECCCCCCCCCCCHHHCCHHHEEEEEECCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHH
VARGRRVAAGVQAIVVPGSGPVKAQAEAEGLDRIFLDAGFEWRLPGCSMCLAMNNDSLNP
HHCCCEEECCEEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVAGRFADIRELN
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA